goatanti mouse igg1  (SouthernBiotech)

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 1. T cell-specific deficiency in UTX blunts allergic sensitization and anaphylaxis in a peanut airway sensitization allergy model. (A) Experimental Timeline for peanutþLPS airway sensitization. (B) Peanut specific IgE and (C) IgG1 at experimental day 14. (D) Core body temperatures recorded after intraperitoneal (i.p) peanut challenge in all mice, (E) Serum mouse mast cell protease (MMCP1) levels following i.p challenge. Data shown are pooled from 5 experiments, with n ¼ 46-49 mice per group. Statistical analysis: Unpaired t-test in (B), (C), and (E) comparing UTXfl/fl and UTX-TCD in each category. Two-way ANOVA In D. P < 0.05, P < 0.01, P < 0.001, P < 0.0001.

Article Snippet: Total IgE and IgG1 were measured by ELISA as previously described.23 96-well plates were coated with 1 μg/ml goatanti mouse IgG1 (Southern Biotech cat. no. 1070-01) for total IgG1 or 2 μg/ml rat anti-mouse IgE (Southern Biotech, cat. no, 1130-01) for total IgE and incubated overnight at 4 �C.

Techniques:

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 3. Frequencies of Th2, but not Tfh cells, in UTX-TCD mice correlate with blunted anaphylaxis and reduced peanut-specific IgE and IgG1. Linear regression analysis comparing Tfh cell frequencies with (A) drop in core body temperature 45 min post challenge, (B) peanut-specific IgE (PNsIgE) at day 14, and (C) peanut-specific IgG1 (PNsIgG1) at day 14. (D) Linear regression analysis comparing CD4þ IL-4þ (Th2) cell frequencies with drop in core body temperature 45 min post challenge, (E) peanut-specific IgE (PNsIgE) at day 14, and (F) peanut-specific IgG1 (PNsIgG1) at day 14. UTXfl/fl control mice are shown in red, and UTX-TCD mice in blue. F-test degree of reduction values (F), R2 and P values for each linear regression analysis are displayed above each plot. Data are from one experiment and are representative of two independent experiments.

Article Snippet: Total IgE and IgG1 were measured by ELISA as previously described.23 96-well plates were coated with 1 μg/ml goatanti mouse IgG1 (Southern Biotech cat. no. 1070-01) for total IgG1 or 2 μg/ml rat anti-mouse IgE (Southern Biotech, cat. no, 1130-01) for total IgE and incubated overnight at 4 �C.

Techniques: Control

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 4. Mice with UTX-deficient T cells skew toward systemic type 2 immune responses, with elevations in both effector T-helper 2 CD4þ T cells and type 2-dependent antibody isotypes, independent of allergic sensitization. (A) Representative flow cytometry plots showing splenic CD44þIL-4þ of CD4þTCRβþ Th2 cells. (B) Summary plots showing frequency, absolute number, and mean fluorescence intensity (MFI) of CD4þCD44þ IL-4þ Th2 cells. (C) Representative flow cytometry plots showing splenic CD44þIL-13þ of CD4þTCRβþ Th2 cells. (D) Summary plots showing frequency, absolute number, and mean fluorescence intensity (MFI) of CD4þCD44þ IL-13þ Th2 cells. (E) Representative flow cytometry showing CD4 and GATA3 staining of gated CD3þ T cells in peripheral blood. (F) Summary plots showing frequency, absolute number, and MFI of CD3þCD4þGATA3þ Th2 cells. (G) Total IgE (n ¼ 62 mice), IgG1 (n ¼ 37-42 mice), and IgG2c (n ¼ 29 mice) at baseline in UTXfl/fl and UTX-TCD mice. Data shown in B and D, except MFI, are pooled from 2 independent experiments, n ¼ 8-9 mice per group. MFI values in B and D and data in E and F are representative of 2 independent experiments with n ¼ 5 mice per group. Statistical analysis: Unpaired t-test in B, D, F, and G, comparing UTXfl/fl and UTX-TCD. P < 0.05, P < 0.01, ns, not significant; Th2, T helper 2.

Article Snippet: Total IgE and IgG1 were measured by ELISA as previously described.23 96-well plates were coated with 1 μg/ml goatanti mouse IgG1 (Southern Biotech cat. no. 1070-01) for total IgG1 or 2 μg/ml rat anti-mouse IgE (Southern Biotech, cat. no, 1130-01) for total IgE and incubated overnight at 4 �C.

Techniques: Flow Cytometry, Fluorescence, Staining