goat polyclonal anti cox2  (Danaher Inc)

Journal: iScience

Article Title: Amphiregulin orchestrates the paracrine immune-suppressive function of amniotic-derived cells through its interplay with COX-2/PGE 2 /EP4 axis

doi: 10.1016/j.isci.2024.110508

Figure Lengend Snippet:

Article Snippet: Goat polyclonal anti-COX2 , Abcam Limited , Cat# AB23672; RRID: AB_731725.

Techniques: Recombinant, Electrophoresis, Protease Inhibitor, Blocking Assay, Luciferase, Proliferation Assay, Apoptosis Assay, Enzyme-linked Immunosorbent Assay, Extraction, Transgenic Assay, Software

Journal: eLife

Article Title: Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2

doi: 10.7554/eLife.81438

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Cox2 (goat polyclonal) , Cayman, 100034-lea , RRID: AB_10078977 , IF (1:200).

Techniques: Software, Staining

Journal: eneuro

Article Title: Microglial TNFα Induces COX2 and PGI2 Synthase Expression in Spinal Endothelial Cells during Neuropathic Pain

doi: 10.1523/eneuro.0064-17.2017

Figure Lengend Snippet: Figure 1. SNI up-regulated Cox2 and Pgis mRNA in the ipsilateral spinal cord. A, Semiquantitative RT-PCR analysis showed Cox2, Pgis, and IP receptor mRNA expression in the L4-L5 ipsilateral spinal cord taken from rats at 0 (naive), 12, 18, 24, 48, and 72 h after nerve injury. GAPDH was used as the loading control. B, Quantification of the relative mRNA levels of Cox2, Pgis, and IP receptor mRNA. The data were first normalized to GAPDH and then expressed as fold change compared with the naive rat spinal cord (n 5, mean SE; , p 0.05; , p 0.001, Fisher’s PLSD). C–K, ISHH images of the spinal cord. Dark-field images of ISHH revealed mRNA distribution of Cox2 (C), Pgis (F), and IP receptor (I) after peripheral nerve injury in the L4–5 spinal cord. Scale bar: (C, F) 500 m, (I) 100 m. Higher-magnification photographs from hematoxylin and eosin–counterstained sections under bright-field illumina- tion in the ipsilateral dorsal horn for Cox2 (D), Pgis (G), and IP receptor (J) of naive rats or 2 d after nerve injury. Arrowheads indicate positive cells. Scale bar: 20 m. E, Bright-field photomicrographs showed combined ISHH for Cox2 mRNA with immunostaining of

Article Snippet: For single immunohistochemistry (IHC) staining of COX2, the sections were preincubated in TBS containing 10% normal horse serum (NHS) for 30 min, followed by incubation in goat anti-COX2 (M-19) polyclonal antibody (1;1000 and 1:2500, Santa Cruz, sc1747, 0.1 mg/mL, RRID: AB_2084976) containing 5% NGS overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Immunostaining

Journal: eneuro

Article Title: Microglial TNFα Induces COX2 and PGI2 Synthase Expression in Spinal Endothelial Cells during Neuropathic Pain

doi: 10.1523/eneuro.0064-17.2017

Figure Lengend Snippet: Figure 2. Up-regulation of COX2 in spinal endothelial cells 48 h after surgery. A–L, Spinal cord sections from SNI at 48 h after surgery in the 4% FA–fixed condition with anti-COX2 antibody at 1:1000 dilution (A–D) and 1:2500 (E–G) and in the 0.4% FA–fixed condition with anti-COX2 antibody at 1:2500 dilution (H–L). Lower-magnification photographs show the ipsilateral (A, E, H) and contralateral (B, F, I) dorsal horn. Higher-magnification photographs from the ipsilateral dorsal horn (C, G, J). J shows COX2-ir cells in blood vessel-like cells; in contrast, the high concentration of the antibody in the 4% FA–fixed condition shows COX2-ir neurons (A–C). COX2

Article Snippet: For single immunohistochemistry (IHC) staining of COX2, the sections were preincubated in TBS containing 10% normal horse serum (NHS) for 30 min, followed by incubation in goat anti-COX2 (M-19) polyclonal antibody (1;1000 and 1:2500, Santa Cruz, sc1747, 0.1 mg/mL, RRID: AB_2084976) containing 5% NGS overnight at 4°C.

Techniques: Concentration Assay

Journal: eneuro

Article Title: Microglial TNFα Induces COX2 and PGI2 Synthase Expression in Spinal Endothelial Cells during Neuropathic Pain

doi: 10.1523/eneuro.0064-17.2017

Figure Lengend Snippet: Figure 3. Intrathecal injection of COX2 inhibitor NS398 attenuated mechanical allodynia induced by SNI. The line graphs show effects of NS398 1 (A), 2 (B), and 7 (C) d after surgery. The single-shot injection of NS398 (5 g/10 l or 50 g/10 l) attenuated mechanical

Article Snippet: For single immunohistochemistry (IHC) staining of COX2, the sections were preincubated in TBS containing 10% normal horse serum (NHS) for 30 min, followed by incubation in goat anti-COX2 (M-19) polyclonal antibody (1;1000 and 1:2500, Santa Cruz, sc1747, 0.1 mg/mL, RRID: AB_2084976) containing 5% NGS overnight at 4°C.

Techniques: Injection

Journal: eneuro

Article Title: Microglial TNFα Induces COX2 and PGI2 Synthase Expression in Spinal Endothelial Cells during Neuropathic Pain

doi: 10.1523/eneuro.0064-17.2017

Figure Lengend Snippet: Figure 8. TNF upregulates expression of COX2 and PGIS in endothelial cells. A, Bright-field photomicrographs show combined ISHH for Tnfr1 and Tnfr2 mRNA with immunostaining of COX2 in the 0.4% FA–fixed condition 24 h after peripheral nerve injury. Scale bar: 20 m. B, The intrathecal TNF injection induced Cox2 and Pgis mRNA expression. Gel panels show RT-PCR products from the L4–5 spinal cord taken from naive rats, 3 h after intrathecal injection of PBS and recombinant TNF. The graphs showed quantification of relative mRNA levels of Cox2 and Pgis. The data were first normalized to GAPDH and then expressed as fold change compared with the naive rat spinal cord (n 5, mean SE, , p 0.05; , p 0.001, Fisher’s PLSD). C, Dark-field photographs of ISHH show Cox2 and Pgis mRNA in the spinal cord 3 h after injection of PBS or recombinant TNF. Right higher-magnification photographs are from hematoxylin and eosin–counterstained sections under bright-field illumination in the ipsilateral dorsal horn. Scale bars: dark-field photographs, 500 m; bright-field photographs, 20 m. D, Effects of the neutralizing TNF antibody on neuropathic pain behaviors. Intrathecal administration of the neutralizing TNF antibody suppressed the mechanical allodynia induced by SNI. In these graphs, values are represented as mean SD (n 6–7 in each group, , p 0.05 compared with vehicle at each time point). Pre, before

Article Snippet: For single immunohistochemistry (IHC) staining of COX2, the sections were preincubated in TBS containing 10% normal horse serum (NHS) for 30 min, followed by incubation in goat anti-COX2 (M-19) polyclonal antibody (1;1000 and 1:2500, Santa Cruz, sc1747, 0.1 mg/mL, RRID: AB_2084976) containing 5% NGS overnight at 4°C.

Techniques: Expressing, Immunostaining, Injection, Reverse Transcription Polymerase Chain Reaction, Recombinant

Journal: eneuro

Article Title: Microglial TNFα Induces COX2 and PGI2 Synthase Expression in Spinal Endothelial Cells during Neuropathic Pain

doi: 10.1523/eneuro.0064-17.2017

Figure Lengend Snippet: Figure 9. Schematic of microglia/endothelial cell/neuron crosstalk supporting developmental neuropathic pain in the spinal dorsal horn. 1, Nerve injury induces TNF in microglia via p-38 MAPK from 18 to 24 h after injury. 2, TNF is released from microglia. 3, TNF induces COX2 and PGIS expression in endothelial cells through TNFR1/TNFR2 signaling from 24 to 48 h after nerve injury. 4, PGI2 is released from endothelial cells. 5, PGI2 binds to IP receptor in spinal neurons and is involved in neuropathic pain.

Article Snippet: For single immunohistochemistry (IHC) staining of COX2, the sections were preincubated in TBS containing 10% normal horse serum (NHS) for 30 min, followed by incubation in goat anti-COX2 (M-19) polyclonal antibody (1;1000 and 1:2500, Santa Cruz, sc1747, 0.1 mg/mL, RRID: AB_2084976) containing 5% NGS overnight at 4°C.

Techniques: Expressing