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goat polyclonal anti contaction 2 tag 1  (R&D Systems)


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    R&D Systems goat polyclonal anti contaction 2 tag 1
    Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with <t>anti-TAG-1</t> antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).
    Goat Polyclonal Anti Contaction 2 Tag 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti contaction 2 tag 1/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat polyclonal anti contaction 2 tag 1 - by Bioz Stars, 2024-12
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    1) Product Images from "Genome Stability by DNA Polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development"

    Article Title: Genome Stability by DNA Polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0665-17.2017

    Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with anti-TAG-1 antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).
    Figure Legend Snippet: Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with anti-TAG-1 antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).

    Techniques Used: Labeling, In Utero, Electroporation, Immunohistochemical staining, Staining, MANN-WHITNEY, Immunohistochemistry, Expressing, Quantitative RT-PCR



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    goat polyclonal anti contaction 2 tag 1  (R&D Systems)
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    R&D Systems goat polyclonal anti contaction 2 tag 1
    Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with <t>anti-TAG-1</t> antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).
    Goat Polyclonal Anti Contaction 2 Tag 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti contaction 2 tag 1/product/R&D Systems
    Average 95 stars, based on 1 article reviews
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    goat polyclonal 173 anti contaction 2 tag 1  (R&D Systems)
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    Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with <t>anti-TAG-1</t> antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).
    Goat Polyclonal 173 Anti Contaction 2 Tag 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with anti-TAG-1 antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).

    Journal: The Journal of Neuroscience

    Article Title: Genome Stability by DNA Polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development

    doi: 10.1523/JNEUROSCI.0665-17.2017

    Figure Lengend Snippet: Polβ deficiency affects corticofugal axonal growth. Cortical layer IV neurons were labeled with EGFP by in utero electroporation at E13.5 and analyzed at E16.5. Immunohistochemical analyses were performed with anti-GFP antibody (A, C, D, F) in Polβfl/fl (A–C) and Emx1-Cre/Polβfl/fl mice (D–F). Nuclei were stained with DAPI (B, C, E, F). Scale bar, 500 μm. Frames indicate electroporated area. Asterisks indicate the center of electroporated area. Arrow and arrowhead indicate tips of corticofugal axons and commissural axons, respectively. G, Quantitative analysis of the length of axons was calculated from the center of each electroporated area to tips of axons in Polβfl/fl and Emx1-Cre/Polβfl/fl mice. **p < 0.01 (Mann–Whitney U test). Values indicate the mean ± SEM from 11 sections of 4 Polβfl/fl brains or 16 sections of 5 Emx1-Cre/Polβfl/fl brains. Immunohistochemistry was performed with anti-TAG-1 antibody (H–O) in E16.5 Polβfl/fl (H–K) and Emx1-Cre/Polβfl/fl (L–O) forebrain. Nuclei were stained with DAPI (I, K, M, O). Scale bar, 500 μm. J, K, N, O, Magnified images of the boxed area in I and M, respectively. Scale bar, 300 μm. IC, Internal capsule. Arrows indicate TAG-1-positive axons. P, Relative gene expression of Gap43, Apc, Tubb6, and Rplp1 was examined by qRT-PCR in E14.5 Polβfl/fl and Emx1-Cre/Polβfl/fl cortices. Gap43 and Apc are known to relate to axonal growth. Tubb6 and Rplp1 are ubiquitously expressed genes. Significant difference from Polβfl/fl cortices: **p < 0.01 (Student's t test).

    Article Snippet: The sections were incubated overnight at 4°C with the following antibodies in buffer G or buffer D: rabbit polyclonal anticleaved caspase-3 (Asp175) (9661S, Cell Signaling Technology) at 1:250, mouse monoclonal anti–neuron-specific β-III tubulin (Tuj1) (MAB1195, R&D Systems) at 1:1000, goat polyclonal anti-Nurr1 (AF2156, R&D Systems) at 1:50, rabbit polyclonal anti-Tbr1 (ab31940, Abcam) at 1:500, rat monoclonal anti-Ctip2 (ab18465, Abcam) at 1:800, rabbit polyclonal anti-CDP (Cux1) (sc-13024, Santa Cruz Biotechnology) at 1:200, rat monoclonal anti-GFP (04404-84, Nacalai Tesque) at 1:1000, goat polyclonal anti-Contaction-2 (TAG-1) (AF4439, R&D Systems) at 1:200, rabbit polyclonal anti-histone H2AX phosphor Ser139 (γH2AX) (39117, Active Motif) at 1:200, rabbit monoclonal anti-Ki67 (RM-9106-S0, Thermo Scientific) at 1:200, or rabbit polyclonal anti-53BP1 (GTX102595, GeneTex) at 1:100.

    Techniques: Labeling, In Utero, Electroporation, Immunohistochemical staining, Staining, MANN-WHITNEY, Immunohistochemistry, Expressing, Quantitative RT-PCR