goat f ab 2 antihuman igg  (Thermo Fisher)


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    Name:
    Goat anti Human IgG F ab 2 Secondary Antibody
    Description:
    Goat anti Human IgG F ab 2 Secondary Antibody for Western Blot IF ICC IHC Flow IP
    Catalog Number:
    31122
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher goat f ab 2 antihuman igg
    Goat anti Human IgG F ab 2 Secondary Antibody for Western Blot IF ICC IHC Flow IP
    https://www.bioz.com/result/goat f ab 2 antihuman igg/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat f ab 2 antihuman igg - by Bioz Stars, 2020-09
    95/100 stars

    Related Products / Commonly Used Together

    2g12 anti-gp120 monoclonal antibody

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    Related Articles

    Staining:

    Article Title: Thymic pathogenicity of an HIV-1 envelope is associated with increased CXCR4 binding efficiency and V5-gp41-dependent activity, but not V1/V2-associated CD4 binding efficiency and viral entry
    Article Snippet: .. To detect surface levels of envelope, 5 × 104 cells were stained with 10 μg/ml 2G12 anti-gp120 monoclonal antibody or an isotype control followed by secondary staining with goat F(ab’)2 antihuman IgG (Fc Sp.)-PE (Caltag). ..

    Article Title: Different Pattern of Immunoglobulin Gene Usage by HIV-1 Compared to Non-HIV-1 Antibodies Derived from the Same Infected Subject
    Article Snippet: .. The cells were harvested 36 hrs post-transfection and incubated with 10 µg/ml of human mAb for 30 minutes at 4°C followed by staining with goat F(ab)2 anti-human IgG (γ) conjugated with PE (Caltag Laboratories, Burlingame, CA). .. The cells were then fixed with 2% paraformaldehyde and analyzed by flow cytometry using a FACSCalibur (Becton Dickinson, San Jose, CA) as described .

    Incubation:

    Article Title: CD11c/CD18 signals very late antigen-4 activation to initiate foamy monocyte recruitment during the onset of hypercholesterolemia
    Article Snippet: .. Goat F(ab')2 anti-human IgG Fc (Pierce) at 100μg/mL was incubated on the glass for 1 h at room temperature. .. Coverslips were then incubated with 5μg/mL of mouse recombinant vascular cell adhesion molecule-1 (mVCAM-1)–Fc chimera (R & D Systems) followed by 0.1% human serum albumin to block nonspecific binding of leukocytes.

    Article Title: Different Pattern of Immunoglobulin Gene Usage by HIV-1 Compared to Non-HIV-1 Antibodies Derived from the Same Infected Subject
    Article Snippet: .. The cells were harvested 36 hrs post-transfection and incubated with 10 µg/ml of human mAb for 30 minutes at 4°C followed by staining with goat F(ab)2 anti-human IgG (γ) conjugated with PE (Caltag Laboratories, Burlingame, CA). .. The cells were then fixed with 2% paraformaldehyde and analyzed by flow cytometry using a FACSCalibur (Becton Dickinson, San Jose, CA) as described .

    Article Title: Heterogeneity and Breadth of Host Antibody Response to KSHV Infection Demonstrated by Systematic Analysis of the KSHV Proteome
    Article Snippet: .. The secondary antibody, a goat F(Ab′)2 Anti-Human IgG (γ), R-PE conjugate, (Life Technologies, Grand Island, NY) was then incubated for 30 minutes; samples were washed, resuspended in 100 µL of assay buffer and analyzed on the Bio-Plex 200 system (Biorad, Hercules, CA). .. The median fluorescence intensity (MFI) across all counted beads was computed for each sample, and recorded after subtracting the background fluorescence.

    Blocking Assay:

    Article Title: Targeting Stereotyped B Cell Receptors from Chronic Lymphocytic Leukemia Patients with Synthetic Antigen Surrogates *
    Article Snippet: .. The TentaGel beads displaying a 4-azidoproline-branched oligomeric library were pre-blocked with 1× TBST Starting Block containing 3% BSA before incubating with 100 n m pooled human IgG (Sigma) and goat F(ab′)2 anti-human IgG Qdot 655 (Life Technologies) for 45 min at room temperature. ..

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  • 90
    Thermo Fisher alexa fluor 488 affinipure goat anti human igg f ab 2 fragment specific
    Alexa Fluor 488 Affinipure Goat Anti Human Igg F Ab 2 Fragment Specific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 affinipure goat anti human igg f ab 2 fragment specific/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 affinipure goat anti human igg f ab 2 fragment specific - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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