goat anti rabbit igg magnetic beads  (New England Biolabs)

Journal: PLoS ONE

Article Title: Potent Adjuvanticity of a Pure TLR7-Agonistic Imidazoquinoline Dendrimer

doi: 10.1371/journal.pone.0043612

Figure Lengend Snippet: Top: Foreground fluoresence intensities of secondary goat anti-rabbit IgG (H+L) conjugated with DyLight680 antibody to each overlapping 13-mer peptide in the microarray are shown (means and SD). Bottom: Raw fluorescence images of representative peptide microarrays are shown. Each array is framed by a fusion tag (Flag) peptide (DYKDDDDKGG, 72 red spots) and influenza virus hemagglutinin (HA) epitope tag peptide (YPYDVPDYAG, 72 green spots) which were used as internal controls.

Article Snippet: After pre-swelling the arrays for 10 min in standard buffer (phosphate-buffered saline [PBS], pH 7.4+0.05% Tween 20) and 60 min in Rockland blocking buffer (Rockland Immunochemicals, Inc., Gilbertsville, PA), the peptide microarrays were initially incubated with the secondary goat anti-rabbit IgG (H+L) conjugated with DyLight680 antibody at a dilution of 1∶5000 for 60 min at room temperature to verify that no significant background interactions occurred with the peptide arrays.

Techniques: Microarray, Fluorescence