Journal: Molecular Medicine Reports

Article Title: Ciliary neurotrophic factor activation of astrocytes mediates neuronal damage via the IL‑6/IL‑6R pathway

doi: 10.3892/mmr.2024.13396

Figure Lengend Snippet: CNTF-ACM promotes the increase of [Ca 2+ ]i and ROS production in neurons. The neurons were labeled with (A) Fluo-3 AM fluorescent probe or (B) DCFH-DA detected by flow cytometry. Analysis of the mean fluorescence intensity of (C) [Ca 2+ ]i and (D) ROS. *P<0.05; **P<0.01. CNTF, ciliary neurotrophic factor; ACM, astrocyte culture medium.

Article Snippet: After washing with PBS, the FITC-labeled goat anti-rabbit IgG fluorescent secondary antibody (cat. no. A0562; 1:200; Beyotime Institute of Biotechnology) was added, incubated at room temperature for 2 h. Nuclei were stained with DAPI (cat. no. C1006; Beyotime Institute of Biotechnology) at room temperature for 5 min and washed with PBS three times for 5 min each.

Techniques: Labeling, Flow Cytometry, Fluorescence