af488 conjugated goat anti rabbit igg h l  (Danaher Inc)

Journal: Cell Communication and Signaling : CCS

Article Title: Human IL-22 receptor-targeted small protein antagonist suppress murine DSS-induced colitis

doi: 10.1186/s12964-024-01846-w

Figure Lengend Snippet: Detection of IL-22R1 expression on transfected HEK293T cells and binding of ABR variants to IL-22R1. Representative images of HEK293T cells expressing human IL-22R1 on the surface. Cells were incubated with biotinylated ABR variants (ABR006, ABR023, ABR027, ABR089, ABR099, ABR102, and ABR167) and stained with Streptavidin-AF568 (STV-AF568 conjugate) and anti-IL-22R1 antibody (anti-IL-22R1-AF488)

Article Snippet: After 1 h incubation, cells were washed, and goat anti-rabbit IgG conjugated with AF488 (Abcam, Cambridge, United Kingdom) was added for next 1 h staining in the dark.

Techniques: Expressing, Transfection, Binding Assay, Incubation, Staining

Journal: Cell Communication and Signaling : CCS

Article Title: Human IL-22 receptor-targeted small protein antagonist suppress murine DSS-induced colitis

doi: 10.1186/s12964-024-01846-w

Figure Lengend Snippet: Fluorescence microscopy images demonstrating ABR variant’s binding to the IL-22R1 on stimulated HaCaT cells. HaCaT cells were stimulated with TNFα and IFNγ for 24 h. Double-staining of ABR proteins detected by Streptavidine-AF568 conjugate (red) and rabbit anti-IL-22R1 pAb-AF488 (green) was carried out

Article Snippet: After 1 h incubation, cells were washed, and goat anti-rabbit IgG conjugated with AF488 (Abcam, Cambridge, United Kingdom) was added for next 1 h staining in the dark.

Techniques: Fluorescence, Microscopy, Binding Assay, Double Staining