Structured Review

Proteintech indirect immunoperoxidase technique
Indirect Immunoperoxidase Technique, supplied by Proteintech, used in various techniques. Bioz Stars score: 89/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/indirect immunoperoxidase technique/product/Proteintech
Average 89 stars, based on 104 article reviews
Price from $9.99 to $1999.99
indirect immunoperoxidase technique - by Bioz Stars, 2020-09
89/100 stars

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Western Blot:

Article Title: Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy
Article Snippet: .. The 5x loading buffer was added in a volume ratio of 1:5, and the samples boiled for 10 min. Later on, the processed protein samples were sequentially added to the wells and were electrophoresed at 80 V for 2 h. The transfer was kept at a 200 mA steady flow for 1.5 h. Antibodies against VEGF (Proteintech, USA, 19003–1-AP), TNFSF11 (Abcam, USA, ab100749), COL1A1 (Proteintech, USA, 14695–1-AP), BGLAP (Abcam, USA, IG272465), GAPDH (Santa Cruz, USA, ab4531), RANKL (Abcam, USA, ab9957), p62 (Proteintech, USA, 55274–1-AP), LC3I/II (Abcam, USA, XY-ABD101), atg5 (Proteintech, USA, 66744–1-Ig), atg7 (Proteintech, USA, 11262–2-AP), and atg12 (Proteintech, USA, 11122–1-AP) were used for the Western blotting. ..

Chloramphenicol Acetyltransferase Assay:

Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7
Article Snippet: .. Antibodies against collagen-I (Cat14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA). .. Anti-E-cadherin antibody (Cat#14472), Anti-phospho-Smad2/3 (p-Smad2/3) (Cat#8828) antibody, Anti-phospho-Smad1/5/9 (p-Smad1/5/9) (Cat#13820) antibody, Anti-total-Smad2/3 (t-Smad2/3) (Cat#8685) antibody, Flag Tag (Cat#14793) and HA Tag (Cat#3724), were purchased from Cell Signaling Technology (Danvers, MA, USA).

other:

Article Title: Costunolide reduces glycolysis-associated activation of hepatic stellate cells via inhibition of hexokinase-2
Article Snippet: Rabbit polyclonal antibodies against α-smooth muscle actin (α-SMA, cat. no. 55135–1-AP), collagen I (cat. no. 14695–1-AP), HK2 (cat. no. 22029–1-AP) and glyceraldehyde phosphate dehydrogenase (GAPDH, Cat. No. 10494–1-AP), and secondary antibody HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (cat. no. SA00001–2) were purchased from Proteintech Group.

SDS Page:

Article Title: Pharmacologic inhibition of RGD‐binding integrins ameliorates fibrosis and improves function following kidney injury. Pharmacologic inhibition of RGD‐binding integrins ameliorates fibrosis and improves function following kidney injury
Article Snippet: .. Protein lysates were run on SDS‐PAGE gels and proteins detected using Collagen 1 (Proteintech, cat# 14695‐1‐AP, 1:3,000) and HDAC2 (Abcam, cat# ab32117, 1:3,000) primary antibodies and peroxidase‐labeled goat anti‐rabbit secondary antibody (Sigma‐Aldrich, cat# A0545, 1:10,000). .. Signal was developed with SuperSignal West Dura (Thermo Fisher).

Blocking Assay:

Article Title: Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction
Article Snippet: .. After blocking with 5% skimmed milk for 1 h at room temperature, the membranes were probed with primary antibodies against collagen I (1:2,000; cat. no. 14695-1-AP; ProteinTech Group, Inc.), TSP-1 (1:500; cat. no. 18304-1-AP; ProteinTech Group, Inc.), VEGF-A (1:1,000; cat. no. 19003-1-AP; ProteinTech Group, Inc.), TGF-β1 (1:500; cat. no. 21898-1-AP; ProteinTech Group, Inc.), phosphorylated (p)-Smad2 (1:1,000; cat. no. 3108; Cell Signaling Technology, Inc., Danvers, MA, USA), Smad2 (1:3,000; cat. no. 12570-1-AP; ProteinTech Group, Inc.), fibronectin (1:1,000; cat. no. 15613-1-AP; ProteinTech Group, Inc.), α-smooth muscle actin (α-SMA) (1:500; cat. no. 55135-1-AP; ProteinTech Group, Inc.), Smad3 (1:1,000; cat. no. bs-3484R; BIOSS, Beijing, China), p-Smad3 (1:1,000; cat. no. bsm-52205R; BIOSS), Smad4 (1:1,000; cat. no. bs-0585R; BIOSS), Smad7 (1:1,000; cat. no. bs-23328R; BIOSS) and β-actin (1:500; cat. no. bsm-33036M; BIOSS) overnight at 4°C. .. Then, the membrane was incubated with HRP-labeled goat anti-rabbit IgG (cat. no. A0208) or goat anti-mouse IgG (cat. no. A0216; both 1:5,000; Beyotime Institute of Biotechnology) at 37°C for 45 min. Proteins were visualized using BeyoECL Plus (Beyotime Institute of Biotechnology).

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    Proteintech biotin conjugated affinipure goat anti rabbit igg
    Biotin Conjugated Affinipure Goat Anti Rabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech antibody biotin conjugated goat anti rabbit igg
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    Bio-Rad goat anti rabbit igg hrp conjugate
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    https://www.bioz.com/result/goat anti rabbit igg hrp conjugate/product/Bio-Rad
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