goat anti mouse igg1 af647  (Thermo Fisher)


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    Structured Review

    Thermo Fisher goat anti mouse igg1 af647
    Goat Anti Mouse Igg1 Af647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg1 af647/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg1 af647 - by Bioz Stars, 2020-09
    90/100 stars

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    Staining:

    Article Title: Ultrahigh accuracy imaging modality for super-localization microscopy
    Article Snippet: .. For LAMP1 staining, the sample was fixed with 1.7% (w/v) paraformaldehyde (PFA) and permeabilized with 0.02% (w/v) saponin, each for 10 min at room temperature; then blocked with 4% BSA (w/v) in PBS, stained with mouse anti-LAMP1 antibody (1:100 dilution; Developmental Studies Hybridoma Bank; clone # H4A3) and then counter-stained with goat anti-mouse IgG Alexa647 (1:1000 dilution; Life Technologies), each for 25 min at room temperature. .. For cell membrane staining, Z310 cells were grown to confluence, then stained with 1 µg/ml cholera toxin B subunit labeled with Alexa647 (Life Technologies) for 15 min on ice and fixed with 1.7% (w/v) PFA for 15 min on ice.

    Incubation:

    Article Title: Epithelial-mesenchymal transition and nuclear β-catenin induced by conditional intestinal disruption of Cdh1 with Apc is E-cadherin EC1 domain dependent
    Article Snippet: .. Labeled and washed cells were then incubated with secondary antibodies, either goat anti-mouse IgG1 Alexa405 or goat anti-mouse IgG2a Alexa647 (Invitrogen, 1:500, 30 min). .. For image stream analysis, ApcΔ/Δ Cdh1+/+ Lgr5CreERT2 and ApcΔ/Δ Cdh1fl/fl Lgr5CreERT2 adenoma organoid cell suspensions were processed, fixed and labeled as above, except for dilutions of mouse anti-β-catenin (BD, 1:500), and secondary antibody, goat anti-mouse IgG1 Alexa647 (Invitrogen, 1:1000).

    Article Title: Gold Nanorod-siRNA Induces Efficient In Vivo Gene Silencing in the Rat Hippocampus
    Article Snippet: .. Sections were incubated with the secondary antibody, goat anti-mouse IgG1-AlexaFluor 647 (1:2,000, Invitrogen), in 1X PBS/1% BSA, 120 min at room temperature in a humidified chamber in the dark. ..

    Article Title: CD27 expression discriminates porcine T helper cells with functionally distinct properties
    Article Snippet: .. In a second incubation step, the following secondary antibodies were added: goat anti-mouse IgG2b-Alexa488, goat anti-mouse IgG2a-Alexa647 (both Life Technologies) and goat anti-mouse IgG1-PE (Southern Biotech). ..

    Article Title: PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner
    Article Snippet: .. Cells were incubated with the primary antibody at 4 °C overnight or at 37 °C for 1 h. Afterwards, cells were again washed thrice for 10 min in PBS and then incubated with the respective secondary antibody for 1 h at 37 °C, i.e., goat-anti-rabbit-IgG-Alexa488 (ThermoFisher, 1:400), goat-anti-mouse-IgG-Alexa488 (ThermoFisher, 1:400), goat-anti-rabbit-IgG-Alexa546 (ThermoFisher, 1:400–1:600), goat-anti-mouse-IgG-Alexa647 (ThermoFisher, 1:400). .. Subsequently, cells were washed thrice for 10 min in PBS and samples were incubated with 0.1 mg/µl Hoechst 33342 for 5 min at RT for staining of nuclei.

    Article Title: Fourteen days of bed rest induces a decline in satellite cell content and robust atrophy of skeletal muscle fibers in middle-aged adults
    Article Snippet: .. The next day, slides were incubated in goat anti-rabbit AF488 (no. , dystrophin), goat anti-mouse IgG2b AF647 (no. , type 1), goat anti-mouse IgG1 AF555 (no. , type 2a; all from Invitrogen). ..

    Binding Assay:

    Article Title: CD27 expression discriminates porcine T helper cells with functionally distinct properties
    Article Snippet: .. Thereafter, binding of primary antibodies was detected by isotype-matched secondary antibodies: goat anti-mouse IgG1-Alexa488, goat anti-mouse IgG2a-Alexa647 (both Life Technologies) and goat anti-mouse IgG2b-Biotin (Southern Biotech, Birmingham, AL, USA). .. Free binding sites of secondary antibodies were blocked with whole mouse IgG molecules (2 μg per sample; Jackson ImmunoResearch, West Grove, PA, USA) before cells were incubated with anti-CD45RC-PerCP-Cy5.5 mAb (see above), LIVE/DEAD® Fixable Aqua dead cell stain (Life Technologies) and one of the following streptavidin conjugates: Streptavidin-Qdot605 (Life Technologies, for PMA/Ionomycin stimulation), Streptavidin-Alexa405 (Life Technologies, for PRRSV restimulation) or Streptavidin-eFluor450 (eBiosciences, for FLUAVsw restimulation).

    Labeling:

    Article Title: Epithelial-mesenchymal transition and nuclear β-catenin induced by conditional intestinal disruption of Cdh1 with Apc is E-cadherin EC1 domain dependent
    Article Snippet: .. Labeled and washed cells were then incubated with secondary antibodies, either goat anti-mouse IgG1 Alexa405 or goat anti-mouse IgG2a Alexa647 (Invitrogen, 1:500, 30 min). .. For image stream analysis, ApcΔ/Δ Cdh1+/+ Lgr5CreERT2 and ApcΔ/Δ Cdh1fl/fl Lgr5CreERT2 adenoma organoid cell suspensions were processed, fixed and labeled as above, except for dilutions of mouse anti-β-catenin (BD, 1:500), and secondary antibody, goat anti-mouse IgG1 Alexa647 (Invitrogen, 1:1000).

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    Thermo Fisher goat anti mouse igg h l alexa647 secondary antibodies
    Goat Anti Mouse Igg H L Alexa647 Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l alexa647 secondary antibodies/product/Thermo Fisher
    Average 99 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l alexa647 secondary antibodies - by Bioz Stars, 2020-09
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    Thermo Fisher alexafluor 647 labeled goat anti rat igg
    Alexafluor 647 Labeled Goat Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexafluor 647 labeled goat anti rat igg/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
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    alexafluor 647 labeled goat anti rat igg - by Bioz Stars, 2020-09
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    Thermo Fisher goat anti mouse igg h l highly cross adsorbed secondary antibody
    Goat Anti Mouse Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l highly cross adsorbed secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 320 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l highly cross adsorbed secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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