goat anti mouse dkk 3  (R&D Systems)

Journal: eLife

Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model

doi: 10.7554/eLife.89453

Figure Lengend Snippet:

Article Snippet: Primary antibodies and dilutions used for western blot were: Mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), goat anti- Human DKK-3 (1:1000, R&D Systems, Cat# AF1118, RRID: AB_354610 ), rabbit anti-GAPDH (1:5000, Abcam, Cat# ab181602, RRID: AB_2630358 ), rabbit anti-GluA1 (1:1000, Cell Signaling Technology, Cat# 13185, RRID: AB_2732897 ), rabbit anti-phospho GluA1 Serine 845 (1:1000, Cell Signaling Technology, Cat# 8084, RRID: AB_10860773 ), rabbit anti- SAPK/JNK (1:1,000, Cell Signaling Technology, Cat# 9252, RRID: AB_2250373 ), mouse anti-Phospho-SAPK/JNK (Thr183/Tyr185) (1:500, Cell Signaling Technology, Cat# 9255, RRID: AB_2307321 ), mouse anti-Tubulin (1:5000, Sigma-Aldrich, Cat# T9026, RRID: AB_477593 ), mouse Anti-Vinculin (1:2000, Sigma Aldrich, Cat# v4505, RRID: AB_477617 ), HRP mouse anti-beta Actin (1:10,000, Abcam, Cat# ab8224, RRID: AB_449644 ).

Techniques: Isolation, Knock-Out, Control, Sequencing, shRNA, Recombinant, TUNEL Assay, cDNA Synthesis, Fluorsave, Software, Microscopy, Immunofluorescence, Patch Clamp

Journal: Cancer Science

Article Title: Dkk‐3 expression in the tumor endothelium: A novel prognostic marker of pancreatic adenocarcinomas

doi: 10.1111/j.1349-7006.2009.01208.x

Figure Lengend Snippet: Immunohistochemical validation of Dkk‐3 and CD‐31 presence on pancreatic adenocarcinoma. Shown are representative tissue sections stained with antibodies against Dkk‐3 (a,c,e) and CD‐31 (b,d,f). Tumor vessels stained positive for Dkk3 and CD31 are shown in (a) and (b). However, not all CD31‐positive vessels were also Dkk‐3‐positive and vice versa (c,d). Tumor epithelial tissue was positive for Dkk‐3 (e, arrows) but not for CD31 staining (a,f, arrows).

Article Snippet: The primary antibodies goat‐antihuman Dkk‐3 (1 μg/mL; R&D Systems) and mouse antihuman CD31 (1:40; DakoCytomation, Glostrup, Denmark) were incubated for 1 h at 37°C in a humidified chamber.

Techniques: Immunohistochemical staining, Biomarker Discovery, Staining