Structured Review

Santa Cruz Biotechnology anti nqo1 mouse monoclonal
DPP3 overexpression promotes NRF2 nuclear accumulation and ROS resistance. ( A ) Levels of DPP3, NRF2 and <t>NQO1</t> proteins in MCF7 cells stably overexpressing wt or mutant DPP3 proteins. GAPDH was used as a loading control. ( B ) Localization of overexpressed DPP3 and endogenous NRF2 in the MCF7 stable cell lines. Immunofluorescence was carried out using anti-HA and anti-NRF2 antibodies for DPP3 and NRF2, respectively. ( C ) ROS levels in the MCF7 stable cell lines. ( D-E ) Sensitivities of the MCF7 stable cell lines to H 2 O 2 ( D ) and diquat ( E ). Cells were treated with indicated concentrations of H 2 O 2 and diquat for 42 hr. Values presented are means from 2 independent experiments. Error bars represent standard deviations (SDs). Statistical significance was calculated by Student's t test comparing the values for the 2 ETGE mutants (T481E and G482E) with those of 3 ETGE-wt proteins (wt, Y318F and E451Q). *p
Anti Nqo1 Mouse Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 87/100, based on 429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nqo1 mouse monoclonal/product/Santa Cruz Biotechnology
Average 87 stars, based on 429 article reviews
Price from $9.99 to $1999.99
anti nqo1 mouse monoclonal - by Bioz Stars, 2020-03
87/100 stars

Images

1) Product Images from "NRF2 induction supporting breast cancer cell survival is enabled by oxidative stress-induced DPP3-KEAP1 interaction"

Article Title: NRF2 induction supporting breast cancer cell survival is enabled by oxidative stress-induced DPP3-KEAP1 interaction

Journal: Cancer research

doi: 10.1158/0008-5472.CAN-16-2204

DPP3 overexpression promotes NRF2 nuclear accumulation and ROS resistance. ( A ) Levels of DPP3, NRF2 and NQO1 proteins in MCF7 cells stably overexpressing wt or mutant DPP3 proteins. GAPDH was used as a loading control. ( B ) Localization of overexpressed DPP3 and endogenous NRF2 in the MCF7 stable cell lines. Immunofluorescence was carried out using anti-HA and anti-NRF2 antibodies for DPP3 and NRF2, respectively. ( C ) ROS levels in the MCF7 stable cell lines. ( D-E ) Sensitivities of the MCF7 stable cell lines to H 2 O 2 ( D ) and diquat ( E ). Cells were treated with indicated concentrations of H 2 O 2 and diquat for 42 hr. Values presented are means from 2 independent experiments. Error bars represent standard deviations (SDs). Statistical significance was calculated by Student's t test comparing the values for the 2 ETGE mutants (T481E and G482E) with those of 3 ETGE-wt proteins (wt, Y318F and E451Q). *p
Figure Legend Snippet: DPP3 overexpression promotes NRF2 nuclear accumulation and ROS resistance. ( A ) Levels of DPP3, NRF2 and NQO1 proteins in MCF7 cells stably overexpressing wt or mutant DPP3 proteins. GAPDH was used as a loading control. ( B ) Localization of overexpressed DPP3 and endogenous NRF2 in the MCF7 stable cell lines. Immunofluorescence was carried out using anti-HA and anti-NRF2 antibodies for DPP3 and NRF2, respectively. ( C ) ROS levels in the MCF7 stable cell lines. ( D-E ) Sensitivities of the MCF7 stable cell lines to H 2 O 2 ( D ) and diquat ( E ). Cells were treated with indicated concentrations of H 2 O 2 and diquat for 42 hr. Values presented are means from 2 independent experiments. Error bars represent standard deviations (SDs). Statistical significance was calculated by Student's t test comparing the values for the 2 ETGE mutants (T481E and G482E) with those of 3 ETGE-wt proteins (wt, Y318F and E451Q). *p

Techniques Used: Over Expression, Stable Transfection, Mutagenesis, Immunofluorescence

2) Product Images from "NRF2 induction supporting breast cancer cell survival is enabled by oxidative stress-induced DPP3-KEAP1 interaction"

Article Title: NRF2 induction supporting breast cancer cell survival is enabled by oxidative stress-induced DPP3-KEAP1 interaction

Journal: Cancer research

doi: 10.1158/0008-5472.CAN-16-2204

Overexpression of DPP3 enhances the stability of KEAP1. ( A ) Levels of NRF2, DPP3, KEAP1 and p62 in MCF7 cell lines overexpressing wt and mutant DPP3 proteins. β-Actin was used a loading control. ( B-C ) Stabilities of KEAP in the MCF7 cell lines harboring the empty vector or overexpressing wt DPP3. Cells were either untreated or treated with 50 μg/ml of cycloheximide for 2, 4 and 6 hr, and the proteins were analyzed by western blotting. The intensities of KEAP1 bands were quantified by the ImageJ software, normalized against those of GAPDH and plotted. B shows a set of representative western blots, and C shows means of the quantified results from 3 independent experiments. Error bars represent SDs. *p
Figure Legend Snippet: Overexpression of DPP3 enhances the stability of KEAP1. ( A ) Levels of NRF2, DPP3, KEAP1 and p62 in MCF7 cell lines overexpressing wt and mutant DPP3 proteins. β-Actin was used a loading control. ( B-C ) Stabilities of KEAP in the MCF7 cell lines harboring the empty vector or overexpressing wt DPP3. Cells were either untreated or treated with 50 μg/ml of cycloheximide for 2, 4 and 6 hr, and the proteins were analyzed by western blotting. The intensities of KEAP1 bands were quantified by the ImageJ software, normalized against those of GAPDH and plotted. B shows a set of representative western blots, and C shows means of the quantified results from 3 independent experiments. Error bars represent SDs. *p

Techniques Used: Over Expression, Mutagenesis, Plasmid Preparation, Western Blot, Software

Related Articles

Synthesized:

Article Title: The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via d-Prostanoid Receptor Signaling *
Article Snippet: .. Anti-CRTH2 goat polyclonal antibody and anti-β-actin mouse monoclonal antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phospho-p44/42 MAP kinase (Thr-202/Tyr-204), anti-p44/42 MAPK (ERK1/2), anti-phospho-p38 MAPK (Thr-180/Tyr-182), anti-phospho-SAPK/JNK (Thr-183/Tyr-185), anti-SAPK/JNK, anti-phospho-RSK1 (Ser-380), anti-RSK1/2/3, anti-phospho-CREB (Ser-133), and anti-CREB antibodies were purchased from Cell Signaling Technology (Beverly, MA). siERK1 (catalogue number sc-29307), siERK2 (catalogue number sc-35335), and negative control small Interfering RNA (siRNA; catalogue number sc-37007) were purchased from Santa Cruz Biotechnology. siDP1 (GenBankTM accession number ) and siCRTH2 (GenBankTM accession number ) were synthesized by Bioneer (Daejeon, Korea). siRSK1 and siCREB were synthesized by Invitrogen. .. An additional siRNA negative control was also utilized (Stealth, catalogue number 12935–300; Invitrogen).

Blocking Assay:

Article Title: VPg-Primed RNA Synthesis of Norovirus RNA-Dependent RNA Polymerases by Using a Novel Cell-Based Assay ▿
Article Snippet: Following washes in TBS-T, membranes were incubated in blocking buffer supplemented with horseradish peroxidase (HRP)-conjugated secondary antibody and developed using the ECL Plus detection system (Amersham, United Kingdom). .. RNase L was detected with anti-RNase L mouse monoclonal antibody (Millipore, Bedford, MA), and β-actin was detected with anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Incubation:

Article Title: VPg-Primed RNA Synthesis of Norovirus RNA-Dependent RNA Polymerases by Using a Novel Cell-Based Assay ▿
Article Snippet: Following washes in TBS-T, membranes were incubated in blocking buffer supplemented with horseradish peroxidase (HRP)-conjugated secondary antibody and developed using the ECL Plus detection system (Amersham, United Kingdom). .. RNase L was detected with anti-RNase L mouse monoclonal antibody (Millipore, Bedford, MA), and β-actin was detected with anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Article Title: Augmentation of gonadotropin-releasing hormone receptor expression in the post-lactational mammary tissues of rats
Article Snippet: .. Membranes were blocked with 5% skim milk (Wako Pure Chemicals, Osaka, Japan) for 1 h at room temperature and then incubated with primary antibodies: anti-GnRH receptor, mouse monoclonal antibody (1:200; Acris Antibodies GmbH, Herford, Germany), or anti-β-actin mouse monoclonal antibody (1:1,000; C4, Santa Cruz Biotechnology, Santa Cruz, CA, USA), overnight at 4°C. ..

Expressing:

Article Title: VPg-Primed RNA Synthesis of Norovirus RNA-Dependent RNA Polymerases by Using a Novel Cell-Based Assay ▿
Article Snippet: Paragraph title: Protein expression analysis. ... RNase L was detected with anti-RNase L mouse monoclonal antibody (Millipore, Bedford, MA), and β-actin was detected with anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Western Blot:

Article Title: Cancer Susceptibility Polymorphism of p53 at Codon 72 Affects Phosphorylation and Degradation of p53 Protein *
Article Snippet: Paragraph title: Western Blotting Analysis and Immunoprecipitation ... The antibodies used in this study were as follows: anti-p53 goat polyclonal antibody (FL393); anti-p21 rabbit polyclonal antibody (C-19); anti-PIG3 (N-20) and PIG3 (C-20) goat polyclonal antibody; anti-Bax (N-20) mouse monoclonal antibody; anti-c-Myc mouse monoclonal antibody (9E10) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA); penta-His antibody (Qiagen, Valencia, CA); anti-p53 mouse monoclonal antibodies PAb1801 and PAb421 and anti-Mdm2 mouse monoclonal antibody (clone IF-2) (Calbiochem); anti-p53 mouse monoclonal antibody (PAb122) (Monosan, Uden, Netherlands); anti-phospho-p53 (Ser-6, -9, -15, -20, -37, and -46) rabbit polyclonal antibodies and anti-phospho-Smad2 (138D4) Ser-465/467 antibody (Cell Signaling, Beverly, MA); anti-CRP1 antibody (BD Transduction Laboratories); and anti-FLAG mouse monoclonal antibody (M2); and anti-tubulin antibody (clone B-5-1-2) (Sigma).

Article Title: Treadmill exercise facilitates recovery of locomotor function through axonal regeneration following spinal cord injury in rats
Article Snippet: Paragraph title: Western blot analysis ... Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology) and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats
Article Snippet: Paragraph title: Western blot analysis ... Proteins were then transferred to nitrocellulose and the blots were probed with anti-Bax rabbit polyclonal antibody (1:1,500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Bcl-2 mouse monoclonal antibody (1:1,500, Santa Cruz Biotechnology), anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-TrkB rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-p-ERK1/2 rabbit monoclonal antibody (1:3,000, Cell Signaling Technology, Beverly, MA, USA), anti-p-JNK rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), anti-p-c-Jun rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation potentiates recovery of locomotor function after spinal cord injury in rats
Article Snippet: Paragraph title: Western blot analysis ... Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-synapsin-I rabbit monoclonal antibody (1:1,000, Cell Signaling Technology, Beverly, MA, USA), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Electroacupuncture pretreatment attenuates cerebral ischemic injury through ?7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats
Article Snippet: Paragraph title: Western Blot Analysis of α7nAChR ... The following primary antibodies were used in this study: anti-α7nAChR rabbit polyclonal antibody (Santa Cruz Biotechnology, Cat# sc-5544, 1:200 dilution) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-130300, 1:1000 dilution).

Article Title: Augmentation of gonadotropin-releasing hormone receptor expression in the post-lactational mammary tissues of rats
Article Snippet: Inguinal mammary tissues were also collected from rats each day from D20 to 24 for western blot analysis. .. Membranes were blocked with 5% skim milk (Wako Pure Chemicals, Osaka, Japan) for 1 h at room temperature and then incubated with primary antibodies: anti-GnRH receptor, mouse monoclonal antibody (1:200; Acris Antibodies GmbH, Herford, Germany), or anti-β-actin mouse monoclonal antibody (1:1,000; C4, Santa Cruz Biotechnology, Santa Cruz, CA, USA), overnight at 4°C.

Article Title: Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Article Snippet: Antibodies, Fluorescence Microscopy, and Immunological Methods The antibodies used in this study were M75 and VII-20 anti-CA IX mouse monoclonals [ ]; anti-XPO1 goat polyclonal (CRM1 C-20, Santa Cruz Biotechnology); anti-UBF mouse monoclonal (F-9, Santa Cruz Biotechnology); anti-HIF1α mouse monoclonal (BD Transduction Laboratories); and anti-β -actin mouse monoclonal (AC-15, Santa Cruz Biotechnology). .. Western blot analysis was carried out on cellular lysates obtained as described [ ]; the latter were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore).

Article Title: The Nucleocapsid Protein and Nonstructural Protein 10 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Enhance CD83 Production via NF-κB and Sp1 Signaling Pathways
Article Snippet: Paragraph title: Western blotting. ... Bound proteins were visualized with a Tanon 5200 chemiluminescence imaging system (Tanon, China). β-Actin was detected with an anti-β-actin mouse monoclonal antibody (1:1,000; Santa Cruz Biotech, CA, USA) to demonstrate equal protein sample loading.

Transfection:

Article Title: VPg-Primed RNA Synthesis of Norovirus RNA-Dependent RNA Polymerases by Using a Novel Cell-Based Assay ▿
Article Snippet: After 24 h of transfection, cells were washed once with 1× phosphate-buffered saline (PBS; pH 7.4) and harvested by gentle scraping into 1× SDS-PAGE sample buffer. .. RNase L was detected with anti-RNase L mouse monoclonal antibody (Millipore, Bedford, MA), and β-actin was detected with anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Protease Inhibitor:

Article Title: Cancer Susceptibility Polymorphism of p53 at Codon 72 Affects Phosphorylation and Degradation of p53 Protein *
Article Snippet: Cells were lysed in lysis buffer containing 50 m m Tris-HCl (pH 8.0), 1% Nonidet P-40, 250 m m NaCl, 50 m m NaF, 1 m m Na3 VO4 , 1 m m protease inhibitor (PMSF, aprotinin, and leupeptin), and 1 m m DDT. .. The antibodies used in this study were as follows: anti-p53 goat polyclonal antibody (FL393); anti-p21 rabbit polyclonal antibody (C-19); anti-PIG3 (N-20) and PIG3 (C-20) goat polyclonal antibody; anti-Bax (N-20) mouse monoclonal antibody; anti-c-Myc mouse monoclonal antibody (9E10) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA); penta-His antibody (Qiagen, Valencia, CA); anti-p53 mouse monoclonal antibodies PAb1801 and PAb421 and anti-Mdm2 mouse monoclonal antibody (clone IF-2) (Calbiochem); anti-p53 mouse monoclonal antibody (PAb122) (Monosan, Uden, Netherlands); anti-phospho-p53 (Ser-6, -9, -15, -20, -37, and -46) rabbit polyclonal antibodies and anti-phospho-Smad2 (138D4) Ser-465/467 antibody (Cell Signaling, Beverly, MA); anti-CRP1 antibody (BD Transduction Laboratories); and anti-FLAG mouse monoclonal antibody (M2); and anti-tubulin antibody (clone B-5-1-2) (Sigma).

Article Title: Electroacupuncture pretreatment attenuates cerebral ischemic injury through ?7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats
Article Snippet: The tissue was homogenized on ice in RIPA lysis buffer (Beyotime, Nantong, China) with 1 × Roche complete protease inhibitor cocktail and 1 mM phenylmethylsulfonyl fluoride (PMSF), and western blot was performed as previously described [ ]. .. The following primary antibodies were used in this study: anti-α7nAChR rabbit polyclonal antibody (Santa Cruz Biotechnology, Cat# sc-5544, 1:200 dilution) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-130300, 1:1000 dilution).

Generated:

Article Title: p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion
Article Snippet: Antibodies and materials Anti-p130Cas rabbit polyclonal (αCas3), anti-phospho-FAK (Tyr 397) mouse monoclonal (EMD Millipore), anti-FAK mouse monoclonal (12G4; Abcam), anti–phospho-Src (Tyr 416) rabbit monoclonal (D49G4; Cell Signaling Technology), anti-Src mouse monoclonal (GD11; EMD Millipore), anti–phospho-p38 (Thr 180/Tyr 182) rabbit monoclonal (3D7; Cell Signaling Technology), anti-p38 rabbit polyclonal (Cell Signaling Technology), anti–phospho-HtrA2/Omi (Ser 142) rabbit polyclonal, anti-HtrA2/Omi goat polyclonal (V-17; Santa Cruz Biotechnology, Inc.), anti-p53 mouse monoclonal (1C12; Cell Signaling Technology), anti–β-actin mouse monoclonal (ACTBD11B7; Santa Cruz Biotechnology, Inc.), anti-COX IV rabbit polyclonal (ab16056; Abcam), anti–caspase-3 rabbit polyclonal (Cell Signaling Technology), anti-cleaved caspase-3 rabbit monoclonal (5A1E; Cell Signaling Technology), anti-Flag mouse monoclonal (M2; Sigma-Aldrich), anti–α-tubulin mouse monoclonal (DM1A; Sigma-Aldrich), and anti-GAPDH mouse monoclonal (6C5; Life Technologies) antibodies were used for immunoblot analysis. .. Anti-p130Cas (αCas3) antibody was generated against a bacterially expressed fragment of p130Cas as described elsewhere ( ).

other:

Article Title: Epstein-Barr virus Latent Membrane Protein LMP1 reduces p53 protein levels independent of the PI3K-Akt pathway
Article Snippet: Antibodies Primary antibodies used in this work were anti-p53 mouse monoclonal antibody (DO-1) (Santa Cruz, California, USA) at 1:1000 dilution, anti-LMP1 mouse monoclonal antibody (DAKO, Denmark) at 1:500 dilution, rat monoclonal EBV LMP2A, Clone 14B7 (E. Kremmer, Institute for Molecular Immunology, Munich) at 1:50 dilution, anti-Akt rabbit polyclonal antibody (Cell Signalling Technology, Danvers, USA) at 1:1000 dilution, anti-ubiquitin rabbit polyclonal antibody (Sigma, Saint Louis, USA) at 1:100 dilution, and anti-β-actin mouse monoclonal antibody (Santa Cruz, California, USA) at 1:1000 which served as a loading control protein.

Article Title: The anti-malarial chloroquine overcomes Primary resistance and restores sensitivity to Trastuzumab in HER2-positive breast cancer
Article Snippet: Anti-β-actin mouse monoclonal antibody (C4, #sc-47778) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Article Title: Foot-and-mouth disease virus infection suppresses autophagy and NF-кB antiviral responses via degradation of ATG5-ATG12 by 3Cpro
Article Snippet: Anti-IKKα rabbit polyclonal antibody (sc-7607), anti-Iκ Bα rabbit polyclonal antibody (sc-371), anti-PKR mouse monoclonal antibody (sc-6284), anti-Lambin B1 mouse monoclonal antibody (sc-377000), anti-α -Tubulin mouse monoclonal antibody (sc-398103), anti-β -actin mouse monoclonal antibody (sc-47778), and anti-p65 mouse monoclonal antibody (sc-8008) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Imaging:

Article Title: The Nucleocapsid Protein and Nonstructural Protein 10 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Enhance CD83 Production via NF-κB and Sp1 Signaling Pathways
Article Snippet: .. Bound proteins were visualized with a Tanon 5200 chemiluminescence imaging system (Tanon, China). β-Actin was detected with an anti-β-actin mouse monoclonal antibody (1:1,000; Santa Cruz Biotech, CA, USA) to demonstrate equal protein sample loading. .. Total RNA was prepared from MoDCs by use of a Qiagen RNeasy kit following the manufacturer's instructions.

Sonication:

Article Title: Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats
Article Snippet: The spinal cord tissues covering approximately 1 cm of the rostral and caudal spinal cord at the injury area were prepared and washed with ice-cold PBS and sonicated in 400–600 mL of Triton lysis buffer. .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-Bax rabbit polyclonal antibody (1:1,500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Bcl-2 mouse monoclonal antibody (1:1,500, Santa Cruz Biotechnology), anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-TrkB rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-p-ERK1/2 rabbit monoclonal antibody (1:3,000, Cell Signaling Technology, Beverly, MA, USA), anti-p-JNK rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), anti-p-c-Jun rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation potentiates recovery of locomotor function after spinal cord injury in rats
Article Snippet: The spinal cord tissues covering approximately 1 cm of the rostral and caudal spinal cord at the injury area were prepared and washed with ice-cold PBS and sonicated in 400–600 mL of Triton lysis buffer. .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-synapsin-I rabbit monoclonal antibody (1:1,000, Cell Signaling Technology, Beverly, MA, USA), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Immunofluorescence:

Article Title: p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion
Article Snippet: Antibodies and materials Anti-p130Cas rabbit polyclonal (αCas3), anti-phospho-FAK (Tyr 397) mouse monoclonal (EMD Millipore), anti-FAK mouse monoclonal (12G4; Abcam), anti–phospho-Src (Tyr 416) rabbit monoclonal (D49G4; Cell Signaling Technology), anti-Src mouse monoclonal (GD11; EMD Millipore), anti–phospho-p38 (Thr 180/Tyr 182) rabbit monoclonal (3D7; Cell Signaling Technology), anti-p38 rabbit polyclonal (Cell Signaling Technology), anti–phospho-HtrA2/Omi (Ser 142) rabbit polyclonal, anti-HtrA2/Omi goat polyclonal (V-17; Santa Cruz Biotechnology, Inc.), anti-p53 mouse monoclonal (1C12; Cell Signaling Technology), anti–β-actin mouse monoclonal (ACTBD11B7; Santa Cruz Biotechnology, Inc.), anti-COX IV rabbit polyclonal (ab16056; Abcam), anti–caspase-3 rabbit polyclonal (Cell Signaling Technology), anti-cleaved caspase-3 rabbit monoclonal (5A1E; Cell Signaling Technology), anti-Flag mouse monoclonal (M2; Sigma-Aldrich), anti–α-tubulin mouse monoclonal (DM1A; Sigma-Aldrich), and anti-GAPDH mouse monoclonal (6C5; Life Technologies) antibodies were used for immunoblot analysis. .. Anti–phospho-p130Cas (Tyr 165) rabbit polyclonal (Cell Signaling Technology) and anti-p53 mouse monoclonal (DO-1; Santa Cruz Biotechnology, Inc.) antibodies were used for immunoblot and immunofluorescence analyses.

Nucleic Acid Electrophoresis:

Article Title: Treadmill exercise facilitates recovery of locomotor function through axonal regeneration following spinal cord injury in rats
Article Snippet: Western blot analysis Protein extracts from spinal cord tissue were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology) and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Fluorescence:

Article Title: Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Article Snippet: .. Antibodies, Fluorescence Microscopy, and Immunological Methods The antibodies used in this study were M75 and VII-20 anti-CA IX mouse monoclonals [ ]; anti-XPO1 goat polyclonal (CRM1 C-20, Santa Cruz Biotechnology); anti-UBF mouse monoclonal (F-9, Santa Cruz Biotechnology); anti-HIF1α mouse monoclonal (BD Transduction Laboratories); and anti-β -actin mouse monoclonal (AC-15, Santa Cruz Biotechnology). .. Western blot analysis was carried out on cellular lysates obtained as described [ ]; the latter were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore).

Microscopy:

Article Title: Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Article Snippet: .. Antibodies, Fluorescence Microscopy, and Immunological Methods The antibodies used in this study were M75 and VII-20 anti-CA IX mouse monoclonals [ ]; anti-XPO1 goat polyclonal (CRM1 C-20, Santa Cruz Biotechnology); anti-UBF mouse monoclonal (F-9, Santa Cruz Biotechnology); anti-HIF1α mouse monoclonal (BD Transduction Laboratories); and anti-β -actin mouse monoclonal (AC-15, Santa Cruz Biotechnology). .. Western blot analysis was carried out on cellular lysates obtained as described [ ]; the latter were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore).

Small Interfering RNA:

Article Title: The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via d-Prostanoid Receptor Signaling *
Article Snippet: .. Anti-CRTH2 goat polyclonal antibody and anti-β-actin mouse monoclonal antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phospho-p44/42 MAP kinase (Thr-202/Tyr-204), anti-p44/42 MAPK (ERK1/2), anti-phospho-p38 MAPK (Thr-180/Tyr-182), anti-phospho-SAPK/JNK (Thr-183/Tyr-185), anti-SAPK/JNK, anti-phospho-RSK1 (Ser-380), anti-RSK1/2/3, anti-phospho-CREB (Ser-133), and anti-CREB antibodies were purchased from Cell Signaling Technology (Beverly, MA). siERK1 (catalogue number sc-29307), siERK2 (catalogue number sc-35335), and negative control small Interfering RNA (siRNA; catalogue number sc-37007) were purchased from Santa Cruz Biotechnology. siDP1 (GenBankTM accession number ) and siCRTH2 (GenBankTM accession number ) were synthesized by Bioneer (Daejeon, Korea). siRSK1 and siCREB were synthesized by Invitrogen. .. An additional siRNA negative control was also utilized (Stealth, catalogue number 12935–300; Invitrogen).

Construct:

Article Title: Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Article Snippet: Antibodies, Fluorescence Microscopy, and Immunological Methods The antibodies used in this study were M75 and VII-20 anti-CA IX mouse monoclonals [ ]; anti-XPO1 goat polyclonal (CRM1 C-20, Santa Cruz Biotechnology); anti-UBF mouse monoclonal (F-9, Santa Cruz Biotechnology); anti-HIF1α mouse monoclonal (BD Transduction Laboratories); and anti-β -actin mouse monoclonal (AC-15, Santa Cruz Biotechnology). .. For fluorescence analysis of the EGFP fusion constructs, cells were fixed with 3% (w/v) paraformaldehyde and 1% (w/v) sucrose in PBS for 20 minutes at room temperature (RT).

SDS Page:

Article Title: VPg-Primed RNA Synthesis of Norovirus RNA-Dependent RNA Polymerases by Using a Novel Cell-Based Assay ▿
Article Snippet: After 24 h of transfection, cells were washed once with 1× phosphate-buffered saline (PBS; pH 7.4) and harvested by gentle scraping into 1× SDS-PAGE sample buffer. .. RNase L was detected with anti-RNase L mouse monoclonal antibody (Millipore, Bedford, MA), and β-actin was detected with anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Article Title: Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Article Snippet: Antibodies, Fluorescence Microscopy, and Immunological Methods The antibodies used in this study were M75 and VII-20 anti-CA IX mouse monoclonals [ ]; anti-XPO1 goat polyclonal (CRM1 C-20, Santa Cruz Biotechnology); anti-UBF mouse monoclonal (F-9, Santa Cruz Biotechnology); anti-HIF1α mouse monoclonal (BD Transduction Laboratories); and anti-β -actin mouse monoclonal (AC-15, Santa Cruz Biotechnology). .. Western blot analysis was carried out on cellular lysates obtained as described [ ]; the latter were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore).

Article Title: The Nucleocapsid Protein and Nonstructural Protein 10 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Enhance CD83 Production via NF-κB and Sp1 Signaling Pathways
Article Snippet: Marc-145 or HEK293T cells were lysed, cellular proteins were separated by 12% SDS-PAGE, and the resolved proteins were electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA). .. Bound proteins were visualized with a Tanon 5200 chemiluminescence imaging system (Tanon, China). β-Actin was detected with an anti-β-actin mouse monoclonal antibody (1:1,000; Santa Cruz Biotech, CA, USA) to demonstrate equal protein sample loading.

Plasmid Preparation:

Article Title: Treadmill exercise facilitates recovery of locomotor function through axonal regeneration following spinal cord injury in rats
Article Snippet: Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology) and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology). .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology) and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats
Article Snippet: Proteins were then transferred to nitrocellulose and the blots were probed with anti-Bax rabbit polyclonal antibody (1:1,500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Bcl-2 mouse monoclonal antibody (1:1,500, Santa Cruz Biotechnology), anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-TrkB rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-p-ERK1/2 rabbit monoclonal antibody (1:3,000, Cell Signaling Technology, Beverly, MA, USA), anti-p-JNK rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), anti-p-c-Jun rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology). .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-Bax rabbit polyclonal antibody (1:1,500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Bcl-2 mouse monoclonal antibody (1:1,500, Santa Cruz Biotechnology), anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-TrkB rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-p-ERK1/2 rabbit monoclonal antibody (1:3,000, Cell Signaling Technology, Beverly, MA, USA), anti-p-JNK rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), anti-p-c-Jun rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation potentiates recovery of locomotor function after spinal cord injury in rats
Article Snippet: Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-synapsin-I rabbit monoclonal antibody (1:1,000, Cell Signaling Technology, Beverly, MA, USA), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology). .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-synapsin-I rabbit monoclonal antibody (1:1,000, Cell Signaling Technology, Beverly, MA, USA), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Negative Control:

Article Title: The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via d-Prostanoid Receptor Signaling *
Article Snippet: .. Anti-CRTH2 goat polyclonal antibody and anti-β-actin mouse monoclonal antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phospho-p44/42 MAP kinase (Thr-202/Tyr-204), anti-p44/42 MAPK (ERK1/2), anti-phospho-p38 MAPK (Thr-180/Tyr-182), anti-phospho-SAPK/JNK (Thr-183/Tyr-185), anti-SAPK/JNK, anti-phospho-RSK1 (Ser-380), anti-RSK1/2/3, anti-phospho-CREB (Ser-133), and anti-CREB antibodies were purchased from Cell Signaling Technology (Beverly, MA). siERK1 (catalogue number sc-29307), siERK2 (catalogue number sc-35335), and negative control small Interfering RNA (siRNA; catalogue number sc-37007) were purchased from Santa Cruz Biotechnology. siDP1 (GenBankTM accession number ) and siCRTH2 (GenBankTM accession number ) were synthesized by Bioneer (Daejeon, Korea). siRSK1 and siCREB were synthesized by Invitrogen. .. An additional siRNA negative control was also utilized (Stealth, catalogue number 12935–300; Invitrogen).

Slice Preparation:

Article Title: Electroacupuncture pretreatment attenuates cerebral ischemic injury through ?7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats
Article Snippet: Briefly, a 4-mm thick coronal brain slice was cut, beginning 6 mm from the anterior tip of the frontal lobe. .. The following primary antibodies were used in this study: anti-α7nAChR rabbit polyclonal antibody (Santa Cruz Biotechnology, Cat# sc-5544, 1:200 dilution) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-130300, 1:1000 dilution).

Immunoprecipitation:

Article Title: Cancer Susceptibility Polymorphism of p53 at Codon 72 Affects Phosphorylation and Degradation of p53 Protein *
Article Snippet: Paragraph title: Western Blotting Analysis and Immunoprecipitation ... The antibodies used in this study were as follows: anti-p53 goat polyclonal antibody (FL393); anti-p21 rabbit polyclonal antibody (C-19); anti-PIG3 (N-20) and PIG3 (C-20) goat polyclonal antibody; anti-Bax (N-20) mouse monoclonal antibody; anti-c-Myc mouse monoclonal antibody (9E10) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA); penta-His antibody (Qiagen, Valencia, CA); anti-p53 mouse monoclonal antibodies PAb1801 and PAb421 and anti-Mdm2 mouse monoclonal antibody (clone IF-2) (Calbiochem); anti-p53 mouse monoclonal antibody (PAb122) (Monosan, Uden, Netherlands); anti-phospho-p53 (Ser-6, -9, -15, -20, -37, and -46) rabbit polyclonal antibodies and anti-phospho-Smad2 (138D4) Ser-465/467 antibody (Cell Signaling, Beverly, MA); anti-CRP1 antibody (BD Transduction Laboratories); and anti-FLAG mouse monoclonal antibody (M2); and anti-tubulin antibody (clone B-5-1-2) (Sigma).

Lysis:

Article Title: Cancer Susceptibility Polymorphism of p53 at Codon 72 Affects Phosphorylation and Degradation of p53 Protein *
Article Snippet: Cells were lysed in lysis buffer containing 50 m m Tris-HCl (pH 8.0), 1% Nonidet P-40, 250 m m NaCl, 50 m m NaF, 1 m m Na3 VO4 , 1 m m protease inhibitor (PMSF, aprotinin, and leupeptin), and 1 m m DDT. .. The antibodies used in this study were as follows: anti-p53 goat polyclonal antibody (FL393); anti-p21 rabbit polyclonal antibody (C-19); anti-PIG3 (N-20) and PIG3 (C-20) goat polyclonal antibody; anti-Bax (N-20) mouse monoclonal antibody; anti-c-Myc mouse monoclonal antibody (9E10) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA); penta-His antibody (Qiagen, Valencia, CA); anti-p53 mouse monoclonal antibodies PAb1801 and PAb421 and anti-Mdm2 mouse monoclonal antibody (clone IF-2) (Calbiochem); anti-p53 mouse monoclonal antibody (PAb122) (Monosan, Uden, Netherlands); anti-phospho-p53 (Ser-6, -9, -15, -20, -37, and -46) rabbit polyclonal antibodies and anti-phospho-Smad2 (138D4) Ser-465/467 antibody (Cell Signaling, Beverly, MA); anti-CRP1 antibody (BD Transduction Laboratories); and anti-FLAG mouse monoclonal antibody (M2); and anti-tubulin antibody (clone B-5-1-2) (Sigma).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats
Article Snippet: The spinal cord tissues covering approximately 1 cm of the rostral and caudal spinal cord at the injury area were prepared and washed with ice-cold PBS and sonicated in 400–600 mL of Triton lysis buffer. .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-Bax rabbit polyclonal antibody (1:1,500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Bcl-2 mouse monoclonal antibody (1:1,500, Santa Cruz Biotechnology), anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-TrkB rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology), anti-p-ERK1/2 rabbit monoclonal antibody (1:3,000, Cell Signaling Technology, Beverly, MA, USA), anti-p-JNK rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), anti-p-c-Jun rabbit monoclonal antibody (1:2,000, Cell Signaling Technology), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Treadmill exercise with bone marrow stromal cells transplantation potentiates recovery of locomotor function after spinal cord injury in rats
Article Snippet: The spinal cord tissues covering approximately 1 cm of the rostral and caudal spinal cord at the injury area were prepared and washed with ice-cold PBS and sonicated in 400–600 mL of Triton lysis buffer. .. Proteins were then transferred to nitrocellulose and the blots were probed with anti-BDNF rabbit polyclonal antibody (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-synapsin-I rabbit monoclonal antibody (1:1,000, Cell Signaling Technology, Beverly, MA, USA), and anti-β-actin mouse monoclonal antibody (1:3,000, Santa Cruz Biotechnology).

Article Title: Electroacupuncture pretreatment attenuates cerebral ischemic injury through ?7 nicotinic acetylcholine receptor-mediated inhibition of high-mobility group box 1 release in rats
Article Snippet: The tissue was homogenized on ice in RIPA lysis buffer (Beyotime, Nantong, China) with 1 × Roche complete protease inhibitor cocktail and 1 mM phenylmethylsulfonyl fluoride (PMSF), and western blot was performed as previously described [ ]. .. The following primary antibodies were used in this study: anti-α7nAChR rabbit polyclonal antibody (Santa Cruz Biotechnology, Cat# sc-5544, 1:200 dilution) and anti-β-actin mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-130300, 1:1000 dilution).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    Santa Cruz Biotechnology goat anti keap1 polyclonal antibody e20
    Goat Anti Keap1 Polyclonal Antibody E20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti keap1 polyclonal antibody e20/product/Santa Cruz Biotechnology
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat anti keap1 polyclonal antibody e20 - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    Image Search Results