go taq buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs go taq buffer
    Go Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/go taq buffer/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    go taq buffer - by Bioz Stars, 2020-04
    86/100 stars

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    Inverse PCR:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: Paragraph title: Inverse PCR ... PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O.

    DNA Ligation:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: .. PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O. .. The PCR program used was 95°C for 5 min. and 94°C 30 sec, followed by 35 cycles (94°C 30 sec, 60°C 30 sec, 72°C 1 min. and 45 sec), and the program ended with 72°C 10 min.

    Ligation:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: .. PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O. .. The PCR program used was 95°C for 5 min. and 94°C 30 sec, followed by 35 cycles (94°C 30 sec, 60°C 30 sec, 72°C 1 min. and 45 sec), and the program ended with 72°C 10 min.

    Size-exclusion Chromatography:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O. .. The PCR program used was 95°C for 5 min. and 94°C 30 sec, followed by 35 cycles (94°C 30 sec, 60°C 30 sec, 72°C 1 min. and 45 sec), and the program ended with 72°C 10 min.

    Construct:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: The enzymes used to digest individual T-DNA constructs at the right or left borders are listed in . .. PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O.

    Purification:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: Digestion products were purified by precipitation with 95% ethanol and 3M sodium acetate and resuspended in 10 µl of TE buffer (pH 8.0). .. PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O.

    Polymerase Chain Reaction:

    Article Title: Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
    Article Snippet: .. PCR reactions (10 µl) were prepared for the ligation products as follows: 2 µl 5× Go Taq buffer (NEB), 1 µl dNTPs (2.5 mM), 0.2 µl Primer1 (10 µM), 0.2 µl Primer2 (10 µM), 0.5 µl DMSO (100%), 0.05 µl Go Taq, 2 µl DNA (ligation), and 4.05 µl H2 O. .. The PCR program used was 95°C for 5 min. and 94°C 30 sec, followed by 35 cycles (94°C 30 sec, 60°C 30 sec, 72°C 1 min. and 45 sec), and the program ended with 72°C 10 min.

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  • 86
    New England Biolabs go taq buffer
    Go Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/go taq buffer/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    go taq buffer - by Bioz Stars, 2020-04
    86/100 stars
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