protein a g magnetic beads  (Thermo Fisher)


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    Name:
    Pierce Protein A G Magnetic Beads
    Description:
    Thermo Scientific Pierce Protein A G Magnetic Beads are high performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators Features of Protein A G Magnetic Beads • High capacity nearly four times higher binding capacity than typical magnetic beads from other suppliers allowing the use of smaller amounts per experiment • Low non specific binding stable pre blocked beads provide clean purification products e g antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix • Flexibility convenience of IgG binding domains of both Protein A and Protein G on one bead • Compatibility beads are compatible with manual and automated applications e g Thermo Scientific KingFisher Instruments • Assay consistency magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes These magnetic beads are coated with genetically engineered Pierce Protein A G a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G This enables capture of antibodies from a wider range of species and isotypes than either protein alone Using our crosslinker chemistry you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments Applications • IP and Co IP experiments see complete kit • Immunoprecipitation for analysis in non reducing conditions • Antibody purification The recombinant Protein A G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G Protein A G contains four Fc binding domains from Protein A and two from Protein G making it a convenient tool for investigating and purifying immunoglobulins Thus Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads
    Catalog Number:
    88802
    Price:
    None
    Applications:
    Magnetic Bead Protein Purification|Protein Biology|Protein Purification|Protein Purification & Isolation
    Category:
    Beads Microspheres
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    Structured Review

    Thermo Fisher protein a g magnetic beads
    Schematic of the miCLIP protocol. Capped (grey circle) and polyadenylated cellular RNA containing both m 6 Am (red triangle) and m 6 A (red circle) is fragmented and incubated with an anti-m 6 A antibody. Following UV-crosslinking, the antibody-RNA complexes are recovered using protein A/G-affinity beads and a 3’-adapter is ligated to the RNA. Antibody-RNA complexes are then purified by transferring to a nitrocellulose membrane and eluted using proteinase K, leaving only a small peptide fragment crosslinked at the m 6 A/m 6 Am site. RNA fragments are reverse transcribed, which results in mutations or truncations at the crosslink site in the resulting cDNA. Finally, the cDNA is circularized, re-linearized and amplified by PCR to generate final libraries for sequencing.
    Thermo Scientific Pierce Protein A G Magnetic Beads are high performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators Features of Protein A G Magnetic Beads • High capacity nearly four times higher binding capacity than typical magnetic beads from other suppliers allowing the use of smaller amounts per experiment • Low non specific binding stable pre blocked beads provide clean purification products e g antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix • Flexibility convenience of IgG binding domains of both Protein A and Protein G on one bead • Compatibility beads are compatible with manual and automated applications e g Thermo Scientific KingFisher Instruments • Assay consistency magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes These magnetic beads are coated with genetically engineered Pierce Protein A G a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G This enables capture of antibodies from a wider range of species and isotypes than either protein alone Using our crosslinker chemistry you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments Applications • IP and Co IP experiments see complete kit • Immunoprecipitation for analysis in non reducing conditions • Antibody purification The recombinant Protein A G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G Protein A G contains four Fc binding domains from Protein A and two from Protein G making it a convenient tool for investigating and purifying immunoglobulins Thus Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads
    https://www.bioz.com/result/protein a g magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 42375 article reviews
    Price from $9.99 to $1999.99
    protein a g magnetic beads - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Transcriptome-wide mapping of m6A and m6Am at single-nucleotide resolution using miCLIP"

    Article Title: Transcriptome-wide mapping of m6A and m6Am at single-nucleotide resolution using miCLIP

    Journal: Current protocols in molecular biology

    doi: 10.1002/cpmb.88

    Schematic of the miCLIP protocol. Capped (grey circle) and polyadenylated cellular RNA containing both m 6 Am (red triangle) and m 6 A (red circle) is fragmented and incubated with an anti-m 6 A antibody. Following UV-crosslinking, the antibody-RNA complexes are recovered using protein A/G-affinity beads and a 3’-adapter is ligated to the RNA. Antibody-RNA complexes are then purified by transferring to a nitrocellulose membrane and eluted using proteinase K, leaving only a small peptide fragment crosslinked at the m 6 A/m 6 Am site. RNA fragments are reverse transcribed, which results in mutations or truncations at the crosslink site in the resulting cDNA. Finally, the cDNA is circularized, re-linearized and amplified by PCR to generate final libraries for sequencing.
    Figure Legend Snippet: Schematic of the miCLIP protocol. Capped (grey circle) and polyadenylated cellular RNA containing both m 6 Am (red triangle) and m 6 A (red circle) is fragmented and incubated with an anti-m 6 A antibody. Following UV-crosslinking, the antibody-RNA complexes are recovered using protein A/G-affinity beads and a 3’-adapter is ligated to the RNA. Antibody-RNA complexes are then purified by transferring to a nitrocellulose membrane and eluted using proteinase K, leaving only a small peptide fragment crosslinked at the m 6 A/m 6 Am site. RNA fragments are reverse transcribed, which results in mutations or truncations at the crosslink site in the resulting cDNA. Finally, the cDNA is circularized, re-linearized and amplified by PCR to generate final libraries for sequencing.

    Techniques Used: Incubation, Purification, Transferring, Amplification, Polymerase Chain Reaction, Sequencing

    2) Product Images from "CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries"

    Article Title: CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries

    Journal: bioRxiv

    doi: 10.1101/2020.04.02.021279

    Schematic representation of the CLUE cloning pipeline. The basic structure of a CLUE oligo is the staggered combination of three DNA adapter pairs flanking a sgRNA. The outermost adapters (large white boxes, PCR 1) enable amplification of the entire oligo pool, the second adapter pair (dark colors, PCR 2) allows for specific amplification of a given sgRNA library and the third adapter pair (small white boxes, PCR 3) is comprised of sequences homologous to the sgRNA expression vector, amenable to Gibson assembly. An oligo pool comprising several sgRNA libraries is initially amplified and cloned into a TOPO vector (PCR 1). From this TOPO pool, specific libraries can be PCR amplified (PCR 2) and in a second step be prepared for Gibson cloning into a sgRNA expression vector (PCR 3) The final product is a specific sgRNA expression library.
    Figure Legend Snippet: Schematic representation of the CLUE cloning pipeline. The basic structure of a CLUE oligo is the staggered combination of three DNA adapter pairs flanking a sgRNA. The outermost adapters (large white boxes, PCR 1) enable amplification of the entire oligo pool, the second adapter pair (dark colors, PCR 2) allows for specific amplification of a given sgRNA library and the third adapter pair (small white boxes, PCR 3) is comprised of sequences homologous to the sgRNA expression vector, amenable to Gibson assembly. An oligo pool comprising several sgRNA libraries is initially amplified and cloned into a TOPO vector (PCR 1). From this TOPO pool, specific libraries can be PCR amplified (PCR 2) and in a second step be prepared for Gibson cloning into a sgRNA expression vector (PCR 3) The final product is a specific sgRNA expression library.

    Techniques Used: Clone Assay, Polymerase Chain Reaction, Amplification, Expressing, Plasmid Preparation

    3) Product Images from "CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries"

    Article Title: CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries

    Journal: bioRxiv

    doi: 10.1101/2020.04.02.021279

    Schematic representation of the CLUE cloning pipeline. The basic structure of a CLUE oligo is the staggered combination of three DNA adapter pairs flanking a sgRNA. The outermost adapters (large white boxes, PCR 1) enable amplification of the entire oligo pool, the second adapter pair (dark colors, PCR 2) allows for specific amplification of a given sgRNA library and the third adapter pair (small white boxes, PCR 3) is comprised of sequences homologous to the sgRNA expression vector, amenable to Gibson assembly. An oligo pool comprising several sgRNA libraries is initially amplified and cloned into a TOPO vector (PCR 1). From this TOPO pool, specific libraries can be PCR amplified (PCR 2) and in a second step be prepared for Gibson cloning into a sgRNA expression vector (PCR 3) The final product is a specific sgRNA expression library.
    Figure Legend Snippet: Schematic representation of the CLUE cloning pipeline. The basic structure of a CLUE oligo is the staggered combination of three DNA adapter pairs flanking a sgRNA. The outermost adapters (large white boxes, PCR 1) enable amplification of the entire oligo pool, the second adapter pair (dark colors, PCR 2) allows for specific amplification of a given sgRNA library and the third adapter pair (small white boxes, PCR 3) is comprised of sequences homologous to the sgRNA expression vector, amenable to Gibson assembly. An oligo pool comprising several sgRNA libraries is initially amplified and cloned into a TOPO vector (PCR 1). From this TOPO pool, specific libraries can be PCR amplified (PCR 2) and in a second step be prepared for Gibson cloning into a sgRNA expression vector (PCR 3) The final product is a specific sgRNA expression library.

    Techniques Used: Clone Assay, Polymerase Chain Reaction, Amplification, Expressing, Plasmid Preparation

    4) Product Images from "KSHV lytic mRNA is efficiently translated in the absence of eIF4F"

    Article Title: KSHV lytic mRNA is efficiently translated in the absence of eIF4F

    Journal: bioRxiv

    doi: 10.1101/356162

    eIF4F disassembly does not deplete viral mRNAs from polysomes. (A) Polysome profile of TRex-BCBLl-RTA cells induced with 1 µg/mL dox for 24 h. Torin or DMSO control were added 2 h prior to harvest and polysome analysis. (B) qRT-PCR analysis of cellular and viral transcripts in polysome fractions. Total RNA was isolated from polysome fractions. RNA was co-precipitated with GlycoBlue and T7-transcribed luciferase RNA to improve and normalize for recovery. RNA was analysed by qRT-PCR for cellular, and viral transcripts. Vertical lines depict the boundaries between the monosomes, light polysome, and heavy polysome fractions (n= 3; means±SEM; statistical significance was determined by two-way ANOVA).
    Figure Legend Snippet: eIF4F disassembly does not deplete viral mRNAs from polysomes. (A) Polysome profile of TRex-BCBLl-RTA cells induced with 1 µg/mL dox for 24 h. Torin or DMSO control were added 2 h prior to harvest and polysome analysis. (B) qRT-PCR analysis of cellular and viral transcripts in polysome fractions. Total RNA was isolated from polysome fractions. RNA was co-precipitated with GlycoBlue and T7-transcribed luciferase RNA to improve and normalize for recovery. RNA was analysed by qRT-PCR for cellular, and viral transcripts. Vertical lines depict the boundaries between the monosomes, light polysome, and heavy polysome fractions (n= 3; means±SEM; statistical significance was determined by two-way ANOVA).

    Techniques Used: Quantitative RT-PCR, Isolation, Luciferase

    Related Articles

    Transfection:

    Article Title: Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells
    Article Snippet: .. 48 h after transfection, cells were lysed in Western blot lysis buffer and PYHIN proteins were immunoprecipitated using anti-HA antibodies (Abcam, #ab18181) and Pierce Protein A/G Magnetic Beads (ThermoFisher). .. To examine the effect of nucleic acids on the interaction between PYHIN proteins and Sp1, cell lysates were treated with benzonase (750 U/ml) in the presence of MgCl2 (1 mM) for 2 hours at room temperature before adding anti-HA antibodies.

    Magnetic Beads:

    Article Title: Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking
    Article Snippet: .. Protein A/G magnetic beads (Pierce) were incubated with the lysate under rotation for 15 min at 22°C; 25 μl of beads were used to precipitate antibodies bound to rhodopsin, while 5 μl of beads were used to precipitate GC-1. .. Flow through was collected and beads were washed in 100 μl of the corresponding lysate buffer before being eluted with 20 μl of 2% sodium dodecyl sulfate (SDS) in PBS.

    Article Title: Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells
    Article Snippet: .. 48 h after transfection, cells were lysed in Western blot lysis buffer and PYHIN proteins were immunoprecipitated using anti-HA antibodies (Abcam, #ab18181) and Pierce Protein A/G Magnetic Beads (ThermoFisher). .. To examine the effect of nucleic acids on the interaction between PYHIN proteins and Sp1, cell lysates were treated with benzonase (750 U/ml) in the presence of MgCl2 (1 mM) for 2 hours at room temperature before adding anti-HA antibodies.

    Article Title: Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase‐2 in hepatocellular carcinoma cells and inhibits cell invasion, et al. Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase‐2 in hepatocellular carcinoma cells and inhibits cell invasion
    Article Snippet: .. Immunoprecipitation was performed using Pierce™ Protein A/G Magnetic Beads (Thermo Fisher) according to the manufacturer's instructions. .. 2.12 Isolation of vesicles and exosomes For vesicle isolation, cells cultured in 15 cm dishes were digested and centrifuged at 1000× g for 5 minutes.

    Article Title: Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins
    Article Snippet: .. The protein-antibody complex was isolated using Pierce Protein A/G Magnetic Beads (Thermo Scientific). .. Samples were analyzed via immunoblot as above.

    Isolation:

    Article Title: Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins
    Article Snippet: .. The protein-antibody complex was isolated using Pierce Protein A/G Magnetic Beads (Thermo Scientific). .. Samples were analyzed via immunoblot as above.

    Centrifugation:

    Article Title: Nuclear Herpesvirus Capsid Motility Is Not Dependent on F-Actin
    Article Snippet: .. Soluble protein was separated from cellular debris by centrifugation, and the resulting lysate was precleared with protein A/G beads (Pierce) for 1 h at 4°C. .. Lysates were then incubated with new beads and polyclonal anti-glutathione S -transferase (GST) (Sigma; G1417) or anti-GFP (Invitrogen; A11122) antibody for 1.5 h at 4°C.

    Immunoprecipitation:

    Article Title: Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells
    Article Snippet: .. 48 h after transfection, cells were lysed in Western blot lysis buffer and PYHIN proteins were immunoprecipitated using anti-HA antibodies (Abcam, #ab18181) and Pierce Protein A/G Magnetic Beads (ThermoFisher). .. To examine the effect of nucleic acids on the interaction between PYHIN proteins and Sp1, cell lysates were treated with benzonase (750 U/ml) in the presence of MgCl2 (1 mM) for 2 hours at room temperature before adding anti-HA antibodies.

    Article Title: Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase‐2 in hepatocellular carcinoma cells and inhibits cell invasion, et al. Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase‐2 in hepatocellular carcinoma cells and inhibits cell invasion
    Article Snippet: .. Immunoprecipitation was performed using Pierce™ Protein A/G Magnetic Beads (Thermo Fisher) according to the manufacturer's instructions. .. 2.12 Isolation of vesicles and exosomes For vesicle isolation, cells cultured in 15 cm dishes were digested and centrifuged at 1000× g for 5 minutes.

    Incubation:

    Article Title: Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking
    Article Snippet: .. Protein A/G magnetic beads (Pierce) were incubated with the lysate under rotation for 15 min at 22°C; 25 μl of beads were used to precipitate antibodies bound to rhodopsin, while 5 μl of beads were used to precipitate GC-1. .. Flow through was collected and beads were washed in 100 μl of the corresponding lysate buffer before being eluted with 20 μl of 2% sodium dodecyl sulfate (SDS) in PBS.

    Article Title: Porcine Reproductive and Respiratory Syndrome Virus Utilizes Viral Apoptotic Mimicry as an Alternative Pathway To Infect Host Cells
    Article Snippet: .. Pulldown assay.The recombinant Fc-fused TIM-1/4 was first bound to protein A/G-beads (Pierce) at 4°C for 4 h. PRRSV virions were subsequently incubated with the beads at 4°C overnight. ..

    other:

    Article Title: Modification of ubiquitin-C-terminal hydrolase-L1 by cyclopentenone prostaglandins exacerbates hypoxic injury
    Article Snippet: Protein A/G beads, NeutrAvidin beads and HRP-conjugated streptavidin (streptavidin-HRP) were from Pierce (Rockford, IL).

    BIA-KA:

    Article Title: A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins
    Article Snippet: .. The amount of IgG in 2 μl of each sera that actually bound to protein A/G beads was estimated by measuring the amount of bead-bound sera released by a low pH glycine elution buffer and measured using the BCA Protein Assay kit (Pierce Biotechnology Inc.). ..

    Western Blot:

    Article Title: Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells
    Article Snippet: .. 48 h after transfection, cells were lysed in Western blot lysis buffer and PYHIN proteins were immunoprecipitated using anti-HA antibodies (Abcam, #ab18181) and Pierce Protein A/G Magnetic Beads (ThermoFisher). .. To examine the effect of nucleic acids on the interaction between PYHIN proteins and Sp1, cell lysates were treated with benzonase (750 U/ml) in the presence of MgCl2 (1 mM) for 2 hours at room temperature before adding anti-HA antibodies.

    Lysis:

    Article Title: Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells
    Article Snippet: .. 48 h after transfection, cells were lysed in Western blot lysis buffer and PYHIN proteins were immunoprecipitated using anti-HA antibodies (Abcam, #ab18181) and Pierce Protein A/G Magnetic Beads (ThermoFisher). .. To examine the effect of nucleic acids on the interaction between PYHIN proteins and Sp1, cell lysates were treated with benzonase (750 U/ml) in the presence of MgCl2 (1 mM) for 2 hours at room temperature before adding anti-HA antibodies.

    Recombinant:

    Article Title: Porcine Reproductive and Respiratory Syndrome Virus Utilizes Viral Apoptotic Mimicry as an Alternative Pathway To Infect Host Cells
    Article Snippet: .. Pulldown assay.The recombinant Fc-fused TIM-1/4 was first bound to protein A/G-beads (Pierce) at 4°C for 4 h. PRRSV virions were subsequently incubated with the beads at 4°C overnight. ..

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    Thermo Fisher glycoblue nucleic acid co precipitant
    Glycoblue Nucleic Acid Co Precipitant, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glycoblue nucleic acid co precipitant/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    glycoblue nucleic acid co precipitant - by Bioz Stars, 2020-09
    99/100 stars
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