Structured Review

GE Healthcare glutathione sepharose fastflow
Glutathione Sepharose Fastflow, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glutathione sepharose fastflow/product/GE Healthcare
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
glutathione sepharose fastflow - by Bioz Stars, 2020-09
99/100 stars

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Related Articles

Flow Cytometry:

Article Title: Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids
Article Snippet: .. Glutathione Sepharose™ fast flow (Amersham Biosciences) was used for initial purification. ..

Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

Article Title: RUTBC2 Protein, a Rab9A Effector and GTPase-activating Protein for Rab36 *
Article Snippet: .. Cleared lysates (19,000 rpm for 30 min at 4 °C in a JA-20 rotor; Beckman Coulter) were incubated with glutathione-Sepharose Fast Flow (GE Healthcare) for 1.5 h at 4 °C. .. The resin was then washed with 25 column volumes lysis buffer and eluted with 50 m m Tris-HCl, pH 8.0, 250 m m NaCl, and 20 m m glutathione.

Recombinant:

Article Title: The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling [OPEN]
Article Snippet: .. Pull-down was performed as described ( ) with the following modifications: Equal volumes of GST or WOX3-His, and ERF3-GST or WOX11-His recombinant proteins were incubated for 6 h at 4°C with 400 μL of GST (GE Healthcare; 17-5132-01) or His (Promega; REF V8500) resin in a total volume of 1 mL of GST or His binding buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5% Lgepal CA-630, and protease inhibitor) for 2 to 3 h at 4°C, and the binding reaction was washed five times (10 min each time at 4°C) by the binding buffer. .. After extensive washing, the pulled down proteins were eluted by boiling, separated on 12% SDS-PAGE, and detected by immunoblots using an anti-GST antibody (abcam; ab19256) and anti-His antibody (abcam; ab9108), respectively.

In Vitro:

Article Title: Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex
Article Snippet: .. Approximately 1 μg of GST protein and GST fusion protein were immobilized with glutathione-Sepharose beads (GE Healthcare, no. 17-5132-01) and incubated together with the in vitro translated proteins in buffer A (40 mM HEPES at pH 7.5, 5 mM MgCl2 , 0.2 mM EDTA, 0.5% Nonidet P40 [NP-40], 100 mM KCl) under rotation for 1 h at 4°C. ..

Chromatography:

Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

Protease Inhibitor:

Article Title: The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling [OPEN]
Article Snippet: .. Pull-down was performed as described ( ) with the following modifications: Equal volumes of GST or WOX3-His, and ERF3-GST or WOX11-His recombinant proteins were incubated for 6 h at 4°C with 400 μL of GST (GE Healthcare; 17-5132-01) or His (Promega; REF V8500) resin in a total volume of 1 mL of GST or His binding buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5% Lgepal CA-630, and protease inhibitor) for 2 to 3 h at 4°C, and the binding reaction was washed five times (10 min each time at 4°C) by the binding buffer. .. After extensive washing, the pulled down proteins were eluted by boiling, separated on 12% SDS-PAGE, and detected by immunoblots using an anti-GST antibody (abcam; ab19256) and anti-His antibody (abcam; ab9108), respectively.

Purification:

Article Title: Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids
Article Snippet: .. Glutathione Sepharose™ fast flow (Amersham Biosciences) was used for initial purification. ..

Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

Incubation:

Article Title: Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex
Article Snippet: .. Approximately 1 μg of GST protein and GST fusion protein were immobilized with glutathione-Sepharose beads (GE Healthcare, no. 17-5132-01) and incubated together with the in vitro translated proteins in buffer A (40 mM HEPES at pH 7.5, 5 mM MgCl2 , 0.2 mM EDTA, 0.5% Nonidet P40 [NP-40], 100 mM KCl) under rotation for 1 h at 4°C. ..

Article Title: The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling [OPEN]
Article Snippet: .. Pull-down was performed as described ( ) with the following modifications: Equal volumes of GST or WOX3-His, and ERF3-GST or WOX11-His recombinant proteins were incubated for 6 h at 4°C with 400 μL of GST (GE Healthcare; 17-5132-01) or His (Promega; REF V8500) resin in a total volume of 1 mL of GST or His binding buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5% Lgepal CA-630, and protease inhibitor) for 2 to 3 h at 4°C, and the binding reaction was washed five times (10 min each time at 4°C) by the binding buffer. .. After extensive washing, the pulled down proteins were eluted by boiling, separated on 12% SDS-PAGE, and detected by immunoblots using an anti-GST antibody (abcam; ab19256) and anti-His antibody (abcam; ab9108), respectively.

Article Title: RUTBC2 Protein, a Rab9A Effector and GTPase-activating Protein for Rab36 *
Article Snippet: .. Cleared lysates (19,000 rpm for 30 min at 4 °C in a JA-20 rotor; Beckman Coulter) were incubated with glutathione-Sepharose Fast Flow (GE Healthcare) for 1.5 h at 4 °C. .. The resin was then washed with 25 column volumes lysis buffer and eluted with 50 m m Tris-HCl, pH 8.0, 250 m m NaCl, and 20 m m glutathione.

Article Title: Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation *
Article Snippet: .. Then proteins were extracted from bacteria using a Triton X-100 containing lysis buffer (50 mm Tris-HCl, pH 7.5, 1 mm EDTA, 100 mm NaCl, 5% glycerol, 0.5% Triton X-100), supplemented with 1 mm DTT and protease inhibitors, and incubated end over end at 4 °C, overnight, with glutathione-Sepharose beads (catalog no. 17-5132-01, lot no. 10172617; GE Healthcare). ..

Lysis:

Article Title: Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation *
Article Snippet: .. Then proteins were extracted from bacteria using a Triton X-100 containing lysis buffer (50 mm Tris-HCl, pH 7.5, 1 mm EDTA, 100 mm NaCl, 5% glycerol, 0.5% Triton X-100), supplemented with 1 mm DTT and protease inhibitors, and incubated end over end at 4 °C, overnight, with glutathione-Sepharose beads (catalog no. 17-5132-01, lot no. 10172617; GE Healthcare). ..

Binding Assay:

Article Title: The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling [OPEN]
Article Snippet: .. Pull-down was performed as described ( ) with the following modifications: Equal volumes of GST or WOX3-His, and ERF3-GST or WOX11-His recombinant proteins were incubated for 6 h at 4°C with 400 μL of GST (GE Healthcare; 17-5132-01) or His (Promega; REF V8500) resin in a total volume of 1 mL of GST or His binding buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5% Lgepal CA-630, and protease inhibitor) for 2 to 3 h at 4°C, and the binding reaction was washed five times (10 min each time at 4°C) by the binding buffer. .. After extensive washing, the pulled down proteins were eluted by boiling, separated on 12% SDS-PAGE, and detected by immunoblots using an anti-GST antibody (abcam; ab19256) and anti-His antibody (abcam; ab9108), respectively.

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    GE Healthcare glutathione sepharose fastflow
    Glutathione Sepharose Fastflow, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose fastflow/product/GE Healthcare
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose fastflow - by Bioz Stars, 2020-09
    99/100 stars
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