Sutter Instrument Company
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Journal: Nature Communications
Article Title: Mechanisms of RCD-1 pore formation and membrane bending
doi: 10.1038/s41467-025-56398-5
Figure Lengend Snippet: AFM topographs of RCD-1 incubated with various lipid membranes of DOPC: DOPS = 8: 2 ( a ) ( a , experimental repeats, n = 10), DOPC: DOPE: DOPS = 6: 2: 2 ( b ), DOPC: DOPE: DOPS = 5: 3: 2 ( c ), PC(22:1): DOPS = 8: 2 ( d ), DOPC: DOPE: DOPA = 6: 2: 2 ( e ), DOPC: DOPE: PA(18:0) = 6: 2: 2 ( f ). b – f Experimental repeats, n = 3. g Illustration of how force spectroscopy was used to measure membrane rigidity. h Bending moduli of the six lipid compositions, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a - f represent the lipid components of ( a – f ). The AFM force spectroscopy experiments included n = 73, 51, 55, 62, 59, and 56 force curves for a - f , respectively. For micropipette aspiration experiments, n = 10, 10, 9, 9, 9, and 10 GUVs for a - f , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.
Article Snippet: Using a
Techniques: Incubation, Force Spectroscopy, Membrane
Journal: Nature Communications
Article Title: Mechanisms of RCD-1 pore formation and membrane bending
doi: 10.1038/s41467-025-56398-5
Figure Lengend Snippet: AFM topographies of RCD-1 incubated with various different fungal membranes: model fungal cell membrane-DOPC: DOPE: DOPA: DOPS: CL(18:1) = 30: 28: 20: 4: 18 ( a ) (weight ratio); model fungal mitochondrial membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 37: 43: 1: 8: 4: 6: 1( b ); model fungal mitochondrial outer membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 40: 36: 4: 9: 5: 5: 1( c ); model fungal mitochondrial inner membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 32: 30: 1: 5: 5: 25: 2( d ); model modified fungal cell membrane- DOPC: DOPE: PA(18:0): DOPS: CL(18:1) = 30: 28: 20: 4: 18( e ); model modified fungal mitochondrial inner membrane- DOPC: DOPE: DOPA: DOPI: DOPS: Heart CL(18:2): DOPG = 32: 30: 1: 5: 5: 25: 2( f ) ( a – f , experimental repeats, n = 3). Membrane bending occurred in the model fungal cell membrane ( a ) and model fungal mitochondrial inner membrane ( d ). Change the PA from 18:1 to 18:0 for artificial fungal cell membrane, bending disappeared in model modified fungal cell membrane ( e ). Change the CL from 18:1 to 18:2 (Heart CL), more bending occurred in the model modified fungal mitochondrial inner membrane ( f ). g Bending moduli of the fungal membranes, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a-f represent the lipid components of ( a – f ), while symbol g represents the yeast extract polar lipid. The AFM force spectroscopy experiments included n = 37, 38, 44, 41, 47, 31, and 37 force curves for a - g , respectively. For micropipette aspiration experiments, n = 9, 9, 10, 10, 9, 10, and 9 GUVs for a - g , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.
Article Snippet: Using a
Techniques: Incubation, Membrane, Modification, Force Spectroscopy