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Eppendorf AG glass micropipette
AFM topographs of RCD-1 incubated with various lipid membranes of DOPC: DOPS = 8: 2 ( a ) ( a , experimental repeats, n = 10), DOPC: DOPE: DOPS = 6: 2: 2 ( b ), DOPC: DOPE: DOPS = 5: 3: 2 ( c ), PC(22:1): DOPS = 8: 2 ( d ), DOPC: DOPE: DOPA = 6: 2: 2 ( e ), DOPC: DOPE: PA(18:0) = 6: 2: 2 ( f ). b – f Experimental repeats, n = 3. g Illustration of how force spectroscopy was used to measure membrane rigidity. h Bending moduli of the six lipid compositions, measured by AFM force spectroscopy (black) and <t>micropipette</t> aspiration (red). Symbols a - f represent the lipid components of ( a – f ). The AFM force spectroscopy experiments included n = 73, 51, 55, 62, 59, and 56 force curves for a - f , respectively. For micropipette aspiration experiments, n = 10, 10, 9, 9, 9, and 10 GUVs for a - f , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.
Glass Micropipette, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AFM topographs of RCD-1 incubated with various lipid membranes of DOPC: DOPS = 8: 2 ( a ) ( a , experimental repeats, n = 10), DOPC: DOPE: DOPS = 6: 2: 2 ( b ), DOPC: DOPE: DOPS = 5: 3: 2 ( c ), PC(22:1): DOPS = 8: 2 ( d ), DOPC: DOPE: DOPA = 6: 2: 2 ( e ), DOPC: DOPE: PA(18:0) = 6: 2: 2 ( f ). b – f Experimental repeats, n = 3. g Illustration of how force spectroscopy was used to measure membrane rigidity. h Bending moduli of the six lipid compositions, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a - f represent the lipid components of ( a – f ). The AFM force spectroscopy experiments included n = 73, 51, 55, 62, 59, and 56 force curves for a - f , respectively. For micropipette aspiration experiments, n = 10, 10, 9, 9, 9, and 10 GUVs for a - f , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.

Journal: Nature Communications

Article Title: Mechanisms of RCD-1 pore formation and membrane bending

doi: 10.1038/s41467-025-56398-5

Figure Lengend Snippet: AFM topographs of RCD-1 incubated with various lipid membranes of DOPC: DOPS = 8: 2 ( a ) ( a , experimental repeats, n = 10), DOPC: DOPE: DOPS = 6: 2: 2 ( b ), DOPC: DOPE: DOPS = 5: 3: 2 ( c ), PC(22:1): DOPS = 8: 2 ( d ), DOPC: DOPE: DOPA = 6: 2: 2 ( e ), DOPC: DOPE: PA(18:0) = 6: 2: 2 ( f ). b – f Experimental repeats, n = 3. g Illustration of how force spectroscopy was used to measure membrane rigidity. h Bending moduli of the six lipid compositions, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a - f represent the lipid components of ( a – f ). The AFM force spectroscopy experiments included n = 73, 51, 55, 62, 59, and 56 force curves for a - f , respectively. For micropipette aspiration experiments, n = 10, 10, 9, 9, 9, and 10 GUVs for a - f , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.

Article Snippet: Using a glass micropipette controlled by a micromanipulator (InjectMan 4, Eppendorf), we followed the previously reported methods for measuring the bending modulus of GUVs – .

Techniques: Incubation, Force Spectroscopy, Membrane

AFM topographies of RCD-1 incubated with various different fungal membranes: model fungal cell membrane-DOPC: DOPE: DOPA: DOPS: CL(18:1) = 30: 28: 20: 4: 18 ( a ) (weight ratio); model fungal mitochondrial membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 37: 43: 1: 8: 4: 6: 1( b ); model fungal mitochondrial outer membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 40: 36: 4: 9: 5: 5: 1( c ); model fungal mitochondrial inner membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 32: 30: 1: 5: 5: 25: 2( d ); model modified fungal cell membrane- DOPC: DOPE: PA(18:0): DOPS: CL(18:1) = 30: 28: 20: 4: 18( e ); model modified fungal mitochondrial inner membrane- DOPC: DOPE: DOPA: DOPI: DOPS: Heart CL(18:2): DOPG = 32: 30: 1: 5: 5: 25: 2( f ) ( a – f , experimental repeats, n = 3). Membrane bending occurred in the model fungal cell membrane ( a ) and model fungal mitochondrial inner membrane ( d ). Change the PA from 18:1 to 18:0 for artificial fungal cell membrane, bending disappeared in model modified fungal cell membrane ( e ). Change the CL from 18:1 to 18:2 (Heart CL), more bending occurred in the model modified fungal mitochondrial inner membrane ( f ). g Bending moduli of the fungal membranes, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a-f represent the lipid components of ( a – f ), while symbol g represents the yeast extract polar lipid. The AFM force spectroscopy experiments included n = 37, 38, 44, 41, 47, 31, and 37 force curves for a - g , respectively. For micropipette aspiration experiments, n = 9, 9, 10, 10, 9, 10, and 9 GUVs for a - g , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.

Journal: Nature Communications

Article Title: Mechanisms of RCD-1 pore formation and membrane bending

doi: 10.1038/s41467-025-56398-5

Figure Lengend Snippet: AFM topographies of RCD-1 incubated with various different fungal membranes: model fungal cell membrane-DOPC: DOPE: DOPA: DOPS: CL(18:1) = 30: 28: 20: 4: 18 ( a ) (weight ratio); model fungal mitochondrial membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 37: 43: 1: 8: 4: 6: 1( b ); model fungal mitochondrial outer membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 40: 36: 4: 9: 5: 5: 1( c ); model fungal mitochondrial inner membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 32: 30: 1: 5: 5: 25: 2( d ); model modified fungal cell membrane- DOPC: DOPE: PA(18:0): DOPS: CL(18:1) = 30: 28: 20: 4: 18( e ); model modified fungal mitochondrial inner membrane- DOPC: DOPE: DOPA: DOPI: DOPS: Heart CL(18:2): DOPG = 32: 30: 1: 5: 5: 25: 2( f ) ( a – f , experimental repeats, n = 3). Membrane bending occurred in the model fungal cell membrane ( a ) and model fungal mitochondrial inner membrane ( d ). Change the PA from 18:1 to 18:0 for artificial fungal cell membrane, bending disappeared in model modified fungal cell membrane ( e ). Change the CL from 18:1 to 18:2 (Heart CL), more bending occurred in the model modified fungal mitochondrial inner membrane ( f ). g Bending moduli of the fungal membranes, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a-f represent the lipid components of ( a – f ), while symbol g represents the yeast extract polar lipid. The AFM force spectroscopy experiments included n = 37, 38, 44, 41, 47, 31, and 37 force curves for a - g , respectively. For micropipette aspiration experiments, n = 9, 9, 10, 10, 9, 10, and 9 GUVs for a - g , respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.

Article Snippet: Using a glass micropipette controlled by a micromanipulator (InjectMan 4, Eppendorf), we followed the previously reported methods for measuring the bending modulus of GUVs – .

Techniques: Incubation, Membrane, Modification, Force Spectroscopy