Structured Review

Addgene inc gitler
Gitler, supplied by Addgene inc, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gitler/product/Addgene inc
Average 78 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gitler - by Bioz Stars, 2020-02
78/100 stars

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Related Articles

Clone Assay:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: The genes encoding modifiers of aSyn toxicity YPT1 , YKT6 , BRE5 , UBP3 , GYP8 , and PMR1 were kindly provided by Dr. Aaron Gitler, Stanford University, cloned in Gateway entry clones and used to generate expression clones on the pRS based gateway vectors pAG305GAL (YPT1 , YKT6 , UBP3 , GYP8 , and PMR1 ) or pAG303GAL (BRE5 ) . .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847).

Fluorescence:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847). .. These clones were used to generate entry clones by recombination cloning into a Gateway pDONR221 vector (Invitrogen).

Microscopy:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847). .. These clones were used to generate entry clones by recombination cloning into a Gateway pDONR221 vector (Invitrogen).

Construct:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847). .. These clones were used to generate entry clones by recombination cloning into a Gateway pDONR221 vector (Invitrogen).

Expressing:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: The genes encoding modifiers of aSyn toxicity YPT1 , YKT6 , BRE5 , UBP3 , GYP8 , and PMR1 were kindly provided by Dr. Aaron Gitler, Stanford University, cloned in Gateway entry clones and used to generate expression clones on the pRS based gateway vectors pAG305GAL (YPT1 , YKT6 , UBP3 , GYP8 , and PMR1 ) or pAG303GAL (BRE5 ) . .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847).

Plasmid Preparation:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: The Pup1p-RFP tag was excised from the original plasmid (kindly provided by Dr. Isabelle Sagot, Institut de Biochimie et Génétique Cellulaires), by SacI/HindIII digestion and inserted in the empty pRS305 plasmid, to generate the pRS305 Pup1p-RFP plasmid. .. The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847).

DNA Sequencing:

Article Title: Phosphorylation Modulates Clearance of Alpha-Synuclein Inclusions in a Yeast Model of Parkinson's Disease
Article Snippet: The vesicles markers Ypt1, Ypt31, Sec4, Ypt6, Vps21, Ypt52 and Ypt7 used on fluorescence microscopy experiments were constructed by Gitler and co-workers and were obtained from Addgene (pAG416GPD-Cerulean-YPT1 , 18848; pAG416GPD-Cerulean-YPT31 , 18849; pAG416GPD-Cerulean-SEC4 , 18844; pAG416GPD-Cerulean-YPT6 , 18845; pAG416GPD-Cerulean-VPS21 , 18842; pAG416GPD-Cerulean-YPT52 , 18843; pAG416GPD-Cerulean-YPT7 , 18847). .. These clones were then used to generate new integrative vectors in the pAG305 GPD-Cerulean-ccdB vector which were verified by DNA sequencing.

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    Addgene inc a gitler
    A Gitler, supplied by Addgene inc, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a gitler/product/Addgene inc
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a gitler - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    78
    Addgene inc gitler
    Gitler, supplied by Addgene inc, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gitler/product/Addgene inc
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gitler - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

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