genomic dna  (Qiagen)


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    Structured Review

    Qiagen genomic dna
    <t>DNA</t> methylation and gene expression detection of ZIC3 and NR2F2 from clinical cases. ( a and c ) Statistical summaries about DNA methylation status of DMRs in ZIC3 and NR2F2 in 20 clinical samples, consisting of five normal providers and fifteen DORV patients. ( b and d ) Diagrams exhibiting average methylated levels of individual <t>CpG</t> sites in DMRs of ZIC3 and NR2F2 from the indicated groups, respectively. ( e and f ) Histograms showing relative gene expression levels of ZIC3 and NR2F2 in different groups of specimens. ( g and h ) Scatterplots showing the gene expression levels of ZIC3 ( g ) and NR2F2 ( h ) are negatively correlated with their promoter methylation status. Pearson’s correlation coefficient and p -values were listed above the plot
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    Images

    1) Product Images from "Genome and epigenome analysis of monozygotic twins discordant for congenital heart disease"

    Article Title: Genome and epigenome analysis of monozygotic twins discordant for congenital heart disease

    Journal: BMC Genomics

    doi: 10.1186/s12864-018-4814-7

    DNA methylation and gene expression detection of ZIC3 and NR2F2 from clinical cases. ( a and c ) Statistical summaries about DNA methylation status of DMRs in ZIC3 and NR2F2 in 20 clinical samples, consisting of five normal providers and fifteen DORV patients. ( b and d ) Diagrams exhibiting average methylated levels of individual CpG sites in DMRs of ZIC3 and NR2F2 from the indicated groups, respectively. ( e and f ) Histograms showing relative gene expression levels of ZIC3 and NR2F2 in different groups of specimens. ( g and h ) Scatterplots showing the gene expression levels of ZIC3 ( g ) and NR2F2 ( h ) are negatively correlated with their promoter methylation status. Pearson’s correlation coefficient and p -values were listed above the plot
    Figure Legend Snippet: DNA methylation and gene expression detection of ZIC3 and NR2F2 from clinical cases. ( a and c ) Statistical summaries about DNA methylation status of DMRs in ZIC3 and NR2F2 in 20 clinical samples, consisting of five normal providers and fifteen DORV patients. ( b and d ) Diagrams exhibiting average methylated levels of individual CpG sites in DMRs of ZIC3 and NR2F2 from the indicated groups, respectively. ( e and f ) Histograms showing relative gene expression levels of ZIC3 and NR2F2 in different groups of specimens. ( g and h ) Scatterplots showing the gene expression levels of ZIC3 ( g ) and NR2F2 ( h ) are negatively correlated with their promoter methylation status. Pearson’s correlation coefficient and p -values were listed above the plot

    Techniques Used: DNA Methylation Assay, Expressing, Methylation

    2) Product Images from "N6-methyladenine DNA Modification in Glioblastoma"

    Article Title: N6-methyladenine DNA Modification in Glioblastoma

    Journal: Cell

    doi: 10.1016/j.cell.2018.10.006

    Identification of N(6)-methyladenine ( N 6 . (A) Levels of the N 6 -mA DNA modification were assessed via DNA dot blot in (1) normal human astrocytes, (2) patient-derived GSC models (387, D456, GSC23, and 1919) and (3) primary human glioblastoma specimens (3028, CW2386) using an N 6 -mA-specific antibody. Methylene blue detected DNA loading. (B) Mass spectrometry analysis of N 6 -mA in two normal human astrocyte cell lines and two patient-derived GSC models (387 and D456). Data are presented as mean ± SD. Two replicates were used for each sample. Significance was determined by one-way ANOVA with Tukey multiple comparison test. P
    Figure Legend Snippet: Identification of N(6)-methyladenine ( N 6 . (A) Levels of the N 6 -mA DNA modification were assessed via DNA dot blot in (1) normal human astrocytes, (2) patient-derived GSC models (387, D456, GSC23, and 1919) and (3) primary human glioblastoma specimens (3028, CW2386) using an N 6 -mA-specific antibody. Methylene blue detected DNA loading. (B) Mass spectrometry analysis of N 6 -mA in two normal human astrocyte cell lines and two patient-derived GSC models (387 and D456). Data are presented as mean ± SD. Two replicates were used for each sample. Significance was determined by one-way ANOVA with Tukey multiple comparison test. P

    Techniques Used: Modification, Dot Blot, Derivative Assay, Mass Spectrometry

    ALKBH1 is a N 6 -mA DNA demethylase in human glioblastoma and contributes to N 6 . (A) N 6 -mA labelled DNA oligonucleotides were treated in a cell-free in vitro demethylase reaction with recombinant human ALKBH1 proteins. Results are depicted by dot blot after treatment of two quantities of substrate DNA oligonucleotides. (B) In vitro demethylation reaction was quantified by LC-MS/MS mass spectrometry following addition of ALKBH1 protein to N 6 -mA labelled DNA oligonucleotides. Data are presented as mean ± standard deviation. (Student’s t-test. ***, P
    Figure Legend Snippet: ALKBH1 is a N 6 -mA DNA demethylase in human glioblastoma and contributes to N 6 . (A) N 6 -mA labelled DNA oligonucleotides were treated in a cell-free in vitro demethylase reaction with recombinant human ALKBH1 proteins. Results are depicted by dot blot after treatment of two quantities of substrate DNA oligonucleotides. (B) In vitro demethylation reaction was quantified by LC-MS/MS mass spectrometry following addition of ALKBH1 protein to N 6 -mA labelled DNA oligonucleotides. Data are presented as mean ± standard deviation. (Student’s t-test. ***, P

    Techniques Used: In Vitro, Recombinant, Dot Blot, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Standard Deviation

    Related Articles

    Sequencing:

    Article Title: Targeted mutagenesis in chicken using CRISPR/Cas9 system
    Article Snippet: The remaining PGCs were then transferred to antibiotic-free medium and allowed to proliferate for transplantation and analysis. .. Genomic DNA sequence analysis Genomic DNA was extracted from PGCs, chicken semen, or shafts of chick feathers using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). .. Genomic fragments containing sgRNA target sites were PCR amplified using MightyAmp DNA polymerase Ver.2 (TaKaRa) with specific primer sets , TA cloned into pGEM-T Easy vector (Promega, Madison, WI), and sequenced with the ABI 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Sample Prep:

    Article Title: Microbiomes Associated With Foods From Plant and Animal Sources
    Article Snippet: .. Preparation of Genomic DNA Genomic DNA was prepared from the bacterial pellets of the microbiomes of masala spice mixes, cilantro, cucumber, mung bean sprouts, and smoked salmon using the DNAeasy Blood and Tissue (Qiagen, Germantown, MD, United States) protocol on the QIAcube (Qiagen, Germantown, MD, United States) automated sample preparation instrumentation. .. 16S rRNA Gene Amplicon Library Preparation and Sequencing 16S rRNA gene library preparation, sequencing, data processing and analyses were performed as described by .

    Isolation:

    Article Title: DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells
    Article Snippet: Expression levels of mRNA were compared to known standard samples and normalized to GAPDH. .. Isolation and Bisulfite Treatment of Genomic DNA Genomic DNA was isolated from ~5 × 106 cells using the QIAamp DNA Mini and Blood Mini kit (Qiagen). .. Genomic DNA (1 μ g) was subjected for bisulfite conversion using EpiTect Bisulfite (Qiagen).

    Article Title: Ultra-Rapid Virological Response, Young Age, Low ?-GT/ALT-Ratio, and Absence of Steatosis Identify a Subgroup of HCV Genotype 3 Patients Who Achieve SVR with IFN-?2a Monotherapy
    Article Snippet: Determination of HCV Genotypes In HCV RNA-positive sera, virus genotyping was performed using the Innolipa HCV II line probe assay (Innogenetics, Ghent, Belgium). .. Isolation of Genomic DNA Genomic DNA was purified from peripheral blood mononuclear cells (PBMCs) through the use of the QIAamp DNA Mini Kit following the manufacturer’s blood and body fluid spin protocol (Qiagen). .. The concentration and the purity of the DNA isolated from PBMCs were determined photometrically by reading the absorbance levels at 260 and 280 nm.

    Article Title: DNA Methylation of Alternative Promoters Directs Tissue Specific Expression of Epac2 Isoforms
    Article Snippet: .. Isolation of Genomic DNA Genomic DNA from mouse tissues, paraffin sections or primary cells was isolated employing the QIAGEN DNeasy kit (Gaithersburg, USA) following the manufacturer’s instructions. .. RNA Isolation and Reverse Transcriptase PCR (RT-PCR) RNA was isolated from materials pre-stored at RNA-later buffer, using the RNeasy Protect Mini Kit (QIAGEN, Gaithersburg, USA).

    DNA Purification:

    Article Title: Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
    Article Snippet: The reverse transcription reaction was performed for 1.5 hours at 37°C and subsequently heat inactivated at 70°C for 10 min. After adding 2 U Ribonuclease H (Invitrogen, Darmstadt, Germany) to each sample to degrade RNA and incubation for 20 min at 37°C, samples were unified, followed by column purification of cDNA by QIAquick Nucleotide Removal Kit (Qiagen, Hilden, Germany). cDNA was eluted with 31.5 μ L elution buffer. .. Preparation of Genomic DNA Genomic DNA was extracted from HaCaT cell pellets containing 107 cells, using AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the protocol “Genomic DNA Purification Protocol from Animal Cells .” DNA concentration and quality were determined photometrically. ..

    Concentration Assay:

    Article Title: Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
    Article Snippet: The reverse transcription reaction was performed for 1.5 hours at 37°C and subsequently heat inactivated at 70°C for 10 min. After adding 2 U Ribonuclease H (Invitrogen, Darmstadt, Germany) to each sample to degrade RNA and incubation for 20 min at 37°C, samples were unified, followed by column purification of cDNA by QIAquick Nucleotide Removal Kit (Qiagen, Hilden, Germany). cDNA was eluted with 31.5 μ L elution buffer. .. Preparation of Genomic DNA Genomic DNA was extracted from HaCaT cell pellets containing 107 cells, using AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the protocol “Genomic DNA Purification Protocol from Animal Cells .” DNA concentration and quality were determined photometrically. ..

    Purification:

    Article Title: Ultra-Rapid Virological Response, Young Age, Low ?-GT/ALT-Ratio, and Absence of Steatosis Identify a Subgroup of HCV Genotype 3 Patients Who Achieve SVR with IFN-?2a Monotherapy
    Article Snippet: Determination of HCV Genotypes In HCV RNA-positive sera, virus genotyping was performed using the Innolipa HCV II line probe assay (Innogenetics, Ghent, Belgium). .. Isolation of Genomic DNA Genomic DNA was purified from peripheral blood mononuclear cells (PBMCs) through the use of the QIAamp DNA Mini Kit following the manufacturer’s blood and body fluid spin protocol (Qiagen). .. The concentration and the purity of the DNA isolated from PBMCs were determined photometrically by reading the absorbance levels at 260 and 280 nm.

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    qiagen genomic dna cfdna 10 ng
    Overview of copy number variations via plasma cell-free <t>DNA</t> analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.
    Genomic Dna Cfdna 10 Ng, supplied by qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen genomic dna gdna
    Amplification curves of RPA reactions carried out with RPA primer set 1 and untreated (blue) or treated (purple) reagents. Untreated reactions spiked with 100 copies of E. coli <t>gDNA</t> (green) exhibited a threshold time of 7.25 min, whereas <t>anti-DNA</t> antibody treated reactions spiked with 100 copies of E. coli gDNA (maroon) exhibited a threshold time of 9.5 min.
    Genomic Dna Gdna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen genomic dna
    qAS-PCR of AITL and PTCL-NOS samples. A, Shown are [mut]/([wt]+[mut]) values for each sample. N, mutation negative determined by MiSeq; P, mutation positive determined by MiSeq; Amp, amplified; PLP, periodate/lysine/paraformaldehyde-fixed; <t>FFPE,</t> formalin-fixed/paraffin-embedded. B, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 original or whole genome-amplified <t>DNA</t> samples, including 43 AITL and 52 PTCL-NOS. Cut-off values were determined as 1.5×10 −2 for [mut]/([wt]+[mut]) by qAS-PCR and as 0.02 for mutant allele frequencies as determined by MiSeq. C, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 DNA samples in a log scales. D, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 13 FFPE PCR-amplicon samples.
    Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen dna genomic dna
    ( A ) Venn diagram shows the differentially methylated genes exposed under each aldehyde exposure. The intersection regions are the number of common differentially methylated genes following exposure to the seven aldehydes. ( B ) Hierarchical clustering of methylated genes that commonly altered <t>DNA</t> methylation in <t>A549</t> cells exposed to the seven aldehydes with a fold-change ≥3.0-fold and p -value
    Dna Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.

    Journal: Translational Oncology

    Article Title: Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study

    doi: 10.1016/j.tranon.2020.100908

    Figure Lengend Snippet: Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.

    Article Snippet: DNA was fragmented into an average size of 300 bp (cfDNA without fragmentation), and then 100 ng of fragmented genomic DNA (cfDNA 10 ng) was used for the preparation of sequencing libraries (NEBnext Ultra II).

    Techniques:

    Amplification curves of RPA reactions carried out with RPA primer set 1 and untreated (blue) or treated (purple) reagents. Untreated reactions spiked with 100 copies of E. coli gDNA (green) exhibited a threshold time of 7.25 min, whereas anti-DNA antibody treated reactions spiked with 100 copies of E. coli gDNA (maroon) exhibited a threshold time of 9.5 min.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay

    doi: 10.3389/fcimb.2018.00237

    Figure Lengend Snippet: Amplification curves of RPA reactions carried out with RPA primer set 1 and untreated (blue) or treated (purple) reagents. Untreated reactions spiked with 100 copies of E. coli gDNA (green) exhibited a threshold time of 7.25 min, whereas anti-DNA antibody treated reactions spiked with 100 copies of E. coli gDNA (maroon) exhibited a threshold time of 9.5 min.

    Article Snippet: Genomic DNA (gDNA) was isolated from E. coli cultures using an UltraClean Microbial DNA Isolation kit (Mo Bio Laboratories; Carlsbad, CA).

    Techniques: Amplification, Recombinase Polymerase Amplification

    (A) RPA amplification curves generated using the primer set 1, 0–10 6 copies of E. coli gDNA (2 μL per reaction; n = 2), and RPA reagents pre-treated with anti-dsDNA antibody-coupled magnetic particles. (B) RPA standard curve was generated using the average threshold times calculated from measurements in (A) ( n = 2). The fecal DNA exhibited an average threshold time of 7. 75 min (solid red line) and an estimated load of 6.3-log copies of bacterial gDNA per reaction (dotted red line) or a total bacterial load of 1.01 × 10 7 bacterial gDNA copies per 15 ng of isolated gDNA.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay

    doi: 10.3389/fcimb.2018.00237

    Figure Lengend Snippet: (A) RPA amplification curves generated using the primer set 1, 0–10 6 copies of E. coli gDNA (2 μL per reaction; n = 2), and RPA reagents pre-treated with anti-dsDNA antibody-coupled magnetic particles. (B) RPA standard curve was generated using the average threshold times calculated from measurements in (A) ( n = 2). The fecal DNA exhibited an average threshold time of 7. 75 min (solid red line) and an estimated load of 6.3-log copies of bacterial gDNA per reaction (dotted red line) or a total bacterial load of 1.01 × 10 7 bacterial gDNA copies per 15 ng of isolated gDNA.

    Article Snippet: Genomic DNA (gDNA) was isolated from E. coli cultures using an UltraClean Microbial DNA Isolation kit (Mo Bio Laboratories; Carlsbad, CA).

    Techniques: Recombinase Polymerase Amplification, Amplification, Generated, Isolation

    RPA standard curve of A. muciniphila gDNA (0-1,000,000 copies; ATCC BAA-835) amplified using real-time SYBR green-RPA and primer set 1 ( n = 3). The average threshold time of a 10-fold dilution of the fecal sample gDNA was 6.05 ± 0.1 min (solid red line, n = 3). The estimated load was 1.48 × 10 5 copies of A. muciniphila gDNA per reaction (dotted red line) or 2.99 × 10 5 copies of A. muciniphila gDNA per 15 ng of DNA isolated from the fecal sample.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay

    doi: 10.3389/fcimb.2018.00237

    Figure Lengend Snippet: RPA standard curve of A. muciniphila gDNA (0-1,000,000 copies; ATCC BAA-835) amplified using real-time SYBR green-RPA and primer set 1 ( n = 3). The average threshold time of a 10-fold dilution of the fecal sample gDNA was 6.05 ± 0.1 min (solid red line, n = 3). The estimated load was 1.48 × 10 5 copies of A. muciniphila gDNA per reaction (dotted red line) or 2.99 × 10 5 copies of A. muciniphila gDNA per 15 ng of DNA isolated from the fecal sample.

    Article Snippet: Genomic DNA (gDNA) was isolated from E. coli cultures using an UltraClean Microbial DNA Isolation kit (Mo Bio Laboratories; Carlsbad, CA).

    Techniques: Recombinase Polymerase Amplification, Amplification, SYBR Green Assay, Isolation

    qAS-PCR of AITL and PTCL-NOS samples. A, Shown are [mut]/([wt]+[mut]) values for each sample. N, mutation negative determined by MiSeq; P, mutation positive determined by MiSeq; Amp, amplified; PLP, periodate/lysine/paraformaldehyde-fixed; FFPE, formalin-fixed/paraffin-embedded. B, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 original or whole genome-amplified DNA samples, including 43 AITL and 52 PTCL-NOS. Cut-off values were determined as 1.5×10 −2 for [mut]/([wt]+[mut]) by qAS-PCR and as 0.02 for mutant allele frequencies as determined by MiSeq. C, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 DNA samples in a log scales. D, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 13 FFPE PCR-amplicon samples.

    Journal: PLoS ONE

    Article Title: Detection of the G17V RHOA Mutation in Angioimmunoblastic T-Cell Lymphoma and Related Lymphomas Using Quantitative Allele-Specific PCR

    doi: 10.1371/journal.pone.0109714

    Figure Lengend Snippet: qAS-PCR of AITL and PTCL-NOS samples. A, Shown are [mut]/([wt]+[mut]) values for each sample. N, mutation negative determined by MiSeq; P, mutation positive determined by MiSeq; Amp, amplified; PLP, periodate/lysine/paraformaldehyde-fixed; FFPE, formalin-fixed/paraffin-embedded. B, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 original or whole genome-amplified DNA samples, including 43 AITL and 52 PTCL-NOS. Cut-off values were determined as 1.5×10 −2 for [mut]/([wt]+[mut]) by qAS-PCR and as 0.02 for mutant allele frequencies as determined by MiSeq. C, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 DNA samples in a log scales. D, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 13 FFPE PCR-amplicon samples.

    Article Snippet: Genomic DNA was extracted from 13 formalin-fixed/paraffin-embedded (FFPE), 47 periodate/lysine/paraformaldehyde (PLP)-fixed, and 228 fresh frozen specimens, using an FFPE tissue kit (QIAGEN, Hilden, Germany) for FFPE and PLP samples and a Puregene DNA blood kit (QIAGEN) for fresh frozen specimens, according to manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Mutagenesis, Amplification, Plasmid Purification, Formalin-fixed Paraffin-Embedded

    ( A ) Venn diagram shows the differentially methylated genes exposed under each aldehyde exposure. The intersection regions are the number of common differentially methylated genes following exposure to the seven aldehydes. ( B ) Hierarchical clustering of methylated genes that commonly altered DNA methylation in A549 cells exposed to the seven aldehydes with a fold-change ≥3.0-fold and p -value

    Journal: Scientific Reports

    Article Title: DNA Methylome Analysis of Saturated Aliphatic Aldehydes in Pulmonary Toxicity

    doi: 10.1038/s41598-018-28813-z

    Figure Lengend Snippet: ( A ) Venn diagram shows the differentially methylated genes exposed under each aldehyde exposure. The intersection regions are the number of common differentially methylated genes following exposure to the seven aldehydes. ( B ) Hierarchical clustering of methylated genes that commonly altered DNA methylation in A549 cells exposed to the seven aldehydes with a fold-change ≥3.0-fold and p -value

    Article Snippet: Preparation of genomic DNA Genomic DNA was extracted from A549 cells exposed to the seven aldehydes using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions.

    Techniques: Methylation, DNA Methylation Assay

    Total genome-wide profiling of DNA methylation of A549 cells exposed to the seven aldehydes compared to vehicle control group (DMSO). The heatmap shows the DNA methylation profiles of aldehydes exposed A549 cells based on hierarchical clustering (Yellow: hypermethylation; Black: hypomethylation).

    Journal: Scientific Reports

    Article Title: DNA Methylome Analysis of Saturated Aliphatic Aldehydes in Pulmonary Toxicity

    doi: 10.1038/s41598-018-28813-z

    Figure Lengend Snippet: Total genome-wide profiling of DNA methylation of A549 cells exposed to the seven aldehydes compared to vehicle control group (DMSO). The heatmap shows the DNA methylation profiles of aldehydes exposed A549 cells based on hierarchical clustering (Yellow: hypermethylation; Black: hypomethylation).

    Article Snippet: Preparation of genomic DNA Genomic DNA was extracted from A549 cells exposed to the seven aldehydes using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions.

    Techniques: Genome Wide, DNA Methylation Assay