genomic dna (Qiagen)
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Structured Review
Qiagen
genomic dna
Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna/product/Qiagen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna/product/Qiagen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
genomic dna - by Bioz Stars,
2021-04
86/100 stars
Images
1) Product Images from "Genome and epigenome analysis of monozygotic twins discordant for congenital heart disease"
Article Title: Genome and epigenome analysis of monozygotic twins discordant for congenital heart disease
Journal: BMC Genomics
doi: 10.1186/s12864-018-4814-7
Figure Legend Snippet: DNA methylation and gene expression detection of ZIC3 and NR2F2 from clinical cases. ( a and c ) Statistical summaries about DNA methylation status of DMRs in ZIC3 and NR2F2 in 20 clinical samples, consisting of five normal providers and fifteen DORV patients. ( b and d ) Diagrams exhibiting average methylated levels of individual CpG sites in DMRs of ZIC3 and NR2F2 from the indicated groups, respectively. ( e and f ) Histograms showing relative gene expression levels of ZIC3 and NR2F2 in different groups of specimens. ( g and h ) Scatterplots showing the gene expression levels of ZIC3 ( g ) and NR2F2 ( h ) are negatively correlated with their promoter methylation status. Pearson’s correlation coefficient and p -values were listed above the plot
Techniques Used: DNA Methylation Assay, Expressing, Methylation
2) Product Images from "N6-methyladenine DNA Modification in Glioblastoma"
Article Title: N6-methyladenine DNA Modification in Glioblastoma
Journal: Cell
doi: 10.1016/j.cell.2018.10.006
Figure Legend Snippet: Identification of N(6)-methyladenine ( N 6 . (A) Levels of the N 6 -mA DNA modification were assessed via DNA dot blot in (1) normal human astrocytes, (2) patient-derived GSC models (387, D456, GSC23, and 1919) and (3) primary human glioblastoma specimens (3028, CW2386) using an N 6 -mA-specific antibody. Methylene blue detected DNA loading. (B) Mass spectrometry analysis of N 6 -mA in two normal human astrocyte cell lines and two patient-derived GSC models (387 and D456). Data are presented as mean ñ SD. Two replicates were used for each sample. Significance was determined by one-way ANOVA with Tukey multiple comparison test. P
Techniques Used: Modification, Dot Blot, Derivative Assay, Mass Spectrometry
Figure Legend Snippet: ALKBH1 is a N 6 -mA DNA demethylase in human glioblastoma and contributes to N 6 . (A) N 6 -mA labelled DNA oligonucleotides were treated in a cell-free in vitro demethylase reaction with recombinant human ALKBH1 proteins. Results are depicted by dot blot after treatment of two quantities of substrate DNA oligonucleotides. (B) In vitro demethylation reaction was quantified by LC-MS/MS mass spectrometry following addition of ALKBH1 protein to N 6 -mA labelled DNA oligonucleotides. Data are presented as mean ñ standard deviation. (StudentâÂÂs t-test. ***, P
Techniques Used: In Vitro, Recombinant, Dot Blot, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Standard Deviation
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![qAS-PCR of AITL and PTCL-NOS samples. A, Shown are [mut]/([wt]+[mut]) values for each sample. N, mutation negative determined by MiSeq; P, mutation positive determined by MiSeq; Amp, amplified; PLP, periodate/lysine/paraformaldehyde-fixed; <t>FFPE,</t> formalin-fixed/paraffin-embedded. B, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 original or whole genome-amplified <t>DNA</t> samples, including 43 AITL and 52 PTCL-NOS. Cut-off values were determined as 1.5×10 −2 for [mut]/([wt]+[mut]) by qAS-PCR and as 0.02 for mutant allele frequencies as determined by MiSeq. C, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 95 DNA samples in a log scales. D, Comparison of [mut]/([wt]+[mut]) values by qAS-PCR and mutant allele frequencies as determined by MiSeq for 13 FFPE PCR-amplicon samples.](https://storage.googleapis.com/bioz_article_images/PMC4195681/pone.0109714.g004.jpg)
