genjet lipid transfection reagents (SignaGen)

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SignaGen genjet lipid transfection reagents
Genjet Lipid Transfection Reagents, supplied by SignaGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genjet lipid transfection reagents/product/SignaGen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

genjet lipid transfection reagents - by Bioz Stars, 2021-03

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Article Title: Tetherin/BST-2 Antagonism by Nef Depends on a Direct Physical Interaction between Nef and Tetherin, and on Clathrin-mediated Endocytosis
Article Snippet: All transfections were performed in duplicate in 24-well plates seeded the day before at 5×104 cells per well, using GenJet Lipid Transfection Reagents (SignaGen Laboratories, Gaithersburg, MD).

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Article Title: Polymorphisms in Rhesus Macaque Tetherin Are Associated with Differences in Acute Viremia in Simian Immunodeficiency Virus Δnef-Infected Animals
Article Snippet: Differences in the amount of plasmid DNA in each transfection were offset by the addition of empty pcDNA3 vector. .. All transfections were performed in 24-well plates using GenJet lipid transfection reagents (SignaGen Laboratories, Gaithersburg, MD) according to the manufacturer's instructions. .. Forty-eight hours posttransfection, the amount of virus released into the cell culture supernatant was measured by SIV p27 antigen-capture ELISA (Advanced Bioscience Laboratories, Inc., Kensington, MD), and virus release was expressed as the percentage of maximal particle release relative to control transfections in the absence of tetherin, as previously described ( , ).

Article Title: BCA2/Rabring7 Targets HIV-1 Gag for Lysosomal Degradation in a Tetherin-Independent Manner
Article Snippet: Differences in the amount of plasmid DNA in each transfection were offset by the addition of empty vector (100 ng), either pcDNA3 or pcDNA5. .. All transfections were performed in duplicate in 24-well plates using GenJet Lipid Transfection Reagents (SignaGen Laboratories, Gaithersburg, MD), and results were confirmed in at least three independent assays. .. Forty-eight hours post-transfection, the amount of virions released into the cell culture supernatant was measured by HIV-1 p24, SIV p27 antigen-capture ELISA (Advanced Bioscience Laboratories, Inc., Kensington, MD) or MuLV p30 core ELISA (Cell Biolabs, San Diego, CA), and virus release was expressed as the percentage of maximal virus release in the absence of BCA2 or tetherin, as previously described , , .

Article Title: Species-Specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2
Article Snippet: To assay for the ability of Vpu and Nef to rescue virus release in the presence of tetherin, 293T cells were co-transfected with; (1) proviral DNA for either SIVmac 239 Δnef or HIV-1 HXB2 (100 ng), (2) pcDNA3-based expression constructs for human tetherin (pcDNA3-hBST2), rhesus macaque tetherin (pcDNA3-rBST2), sooty mangabey tetherin (pcDNA3-sBST2) or an empty vector control (pcDNA3) (50 ng), and (3) expression constructs for Vpu (pcDNA-Vphu), Nef (pCGCG-239-Nef and derivatives) or vector alone (pCGCG) (100 ng). .. Transfections were performed in duplicate in 24-well plates seeded the day before at 5×104 cells per well using GenJet Lipid Transfection Reagents (SignaGen Laboratories, Gaithersburg, MD). .. Forty-eight hours after transfection, the amount of virus released into the cell culture supernatant was measured by SIV p27 and HIV-1 p24 antigen-capture ELISA (Advanced Bioscience Laboratories, Inc., Kensington, MD).

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