generuler ultra low range dna ladder  (Thermo Fisher)


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    Name:
    GeneRuler Ultra Low Range DNA Ladder
    Description:
    Thermo Scientific GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a double stranded DNA in the range of 10 bp to 300 bp on agarose or polyacrylamide gels The DNA ladder consists of 11 DNA fragments and is provided with 6X TriTrack DNA Loading Dye Highlights of Ultra Low Range DNA Ladder • Easy visualization three color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye contains Xylene Cyanol FF Bromophenol Blue and Orange G • Simplicity one loading dye for a variety of samples • Sharp bands clear results with mixtures of individual chromatography purified DNA fragments Product use This double stranded DNA ladder is compatible with 4 5 standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining A uniform intensity of bands and bright reference bands allow easy sizing and orientation A defined amount of DNA in each band enables approximate quantification of sample DNA
    Catalog Number:
    sm1211
    Price:
    None
    Applications:
    Agarose Gel Electrophoresis|DNA & RNA Purification & Analysis|Nucleic Acid Gel Electrophoresis & Blotting
    Category:
    Standards Ladders Controls
    Buy from Supplier


    Structured Review

    Thermo Fisher generuler ultra low range dna ladder
    Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. <t>Generuler</t> ultra low range <t>DNA</t> ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.
    Thermo Scientific GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a double stranded DNA in the range of 10 bp to 300 bp on agarose or polyacrylamide gels The DNA ladder consists of 11 DNA fragments and is provided with 6X TriTrack DNA Loading Dye Highlights of Ultra Low Range DNA Ladder • Easy visualization three color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye contains Xylene Cyanol FF Bromophenol Blue and Orange G • Simplicity one loading dye for a variety of samples • Sharp bands clear results with mixtures of individual chromatography purified DNA fragments Product use This double stranded DNA ladder is compatible with 4 5 standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining A uniform intensity of bands and bright reference bands allow easy sizing and orientation A defined amount of DNA in each band enables approximate quantification of sample DNA
    https://www.bioz.com/result/generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    generuler ultra low range dna ladder - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells"

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

    Journal: Scientific Reports

    doi: 10.1038/srep13567

    Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. Generuler ultra low range DNA ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.
    Figure Legend Snippet: Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. Generuler ultra low range DNA ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Staining

    2) Product Images from "A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays"

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    Journal: BMC Medical Genetics

    doi: 10.1186/1471-2350-13-34

    Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.
    Figure Legend Snippet: Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Multiplex Assay

    Agarose gel with PCR products from multiplex PCR for MTHFR 677 C > T , eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lane 2 contains multiplex PCR products with band sizes 178 bp, 248 bp and 371 bp; Lane 3 contains a positive control for eNOS +894 G > T with band size 371 bp; Lane 4 contains a positive control for MTHFR 677 C > T with band size 248 bp; Lane 5 contains a positive control for eNOS −786 T > C with band size 178; and Lane 6 is a negative control.
    Figure Legend Snippet: Agarose gel with PCR products from multiplex PCR for MTHFR 677 C > T , eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lane 2 contains multiplex PCR products with band sizes 178 bp, 248 bp and 371 bp; Lane 3 contains a positive control for eNOS +894 G > T with band size 371 bp; Lane 4 contains a positive control for MTHFR 677 C > T with band size 248 bp; Lane 5 contains a positive control for eNOS −786 T > C with band size 178; and Lane 6 is a negative control.

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Multiplex Assay, Positive Control, Negative Control

    Related Articles

    Amplification:

    Article Title: Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus
    Article Snippet: For amplicon separation and visualization, a 12% polyacrylamide gel was prepared. .. Lanes were loaded with either GeneRuler Ultra Low Range DNA Ladder (Thermo Fisher Scientific), or the samples.

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: Amplification was done with the lowest possible number of cycles ( < 14 cycles for each step) to minimize biased amplification of barcodes. .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA).

    Article Title: Isolation and characterization of genomic microsatellite markers for small cardamom (Elettaria cardamomum Maton) for utility in genetic diversity analysis
    Article Snippet: .. Amplified products were resolved in a 15 % denaturing polyacrylamide gel along with GeneRuler™100 bp and GeneRuler™ ultra low range DNA ladder (Thermoscientific, USA). .. The gels were silver stained (Bassam and Gresshoff ) to view the bands and images were saved by scanning the gels.

    DNA Synthesis:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker. .. To approximate the average polymer length and rate of DNA synthesis, the individual RCA products were cut and released from the surface of a non-patterned substrate using a restriction enzyme that cuts only at the base of each polymer.

    Synthesized:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: To quantify the amount of DNA polymer synthesized in a given channel, the RCA product generated from Circle2 on a non-patterned surface was digested in situ using a restriction enzyme which cuts only once in each repeat unit of the RCA product. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Blocking Assay:

    Article Title: Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum
    Article Snippet: Hybridizations were performed in standard buffer (5x SSC, 0.1% (w/v) N-lauroylsarcosine, 0.02% (w/v) SDS, 1% Blocking Reagent (Merck)) at 60°C overnight. .. GeneRule Ultra Low Range DNA Ladder (ThermoFisher Scientific) was labeled the same way as labeling oligo 102 and used as size standard.

    Electrophoresis:

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays
    Article Snippet: Agarose gel electrophoresis High-resolution agarose gels (5%) (Bioline®, USA) were prepared and immersed into an electrophoresis gel chamber containing 1 X TAE buffer (Fluka, USA). .. Fermentas 6 x Orange DNA Loading Dye® (1 μL) (Fermentas, Lithuania) was mixed with 1 μL of 100 X GelStar® (Lonza, USA) and 1 μL of Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder [this consisted of 10 mM Tris–HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF and 10% glycerol (Fermentas, Lithuania)] or DNA sample on Parafilm® (Parafilm, USA) before adding into the well.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments. .. Electrophoresis was carried out at a constant voltage of 120 V for 90 min.

    Article Title: A DNA nanoscope via auto-cycling proximity recording
    Article Snippet: Paragraph title: Electrophoresis ... All ladders shown are 10–300 nt (Thermo Scientific, Cat. No. SM1213).

    Incubation:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Each aliquot was then incubated for three hours with rotation at room temperature in 1 mL of either EDTA, neutral phosphate or acidic phosphate.

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The channels were washed briefly with a reaction buffer (0.050 m potassium acetate, 0.020 m Tris acetate, 0.01 m magnesium acetate) and an enzyme solution containing 1 U · μL−1 BanI (Cat. # R0118L, New England Biolabs) in 0.050 m potassium acetate, 0.020 m Tris acetate, 0.010 m magnesium acetate, 100 μg · mL−1 BSA, and 10−3 m dithiothreitol, pH = 7.9 was introduced into the chamber and the chip was incubated at 37 °C for ≈12 h. The digestion solution was collected from the chamber and analyzed using polyacrylamide gel electrophoresis. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Expressing:

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: At 72 hours, cells were analyzed for RFP expression, and half were snap frozen for use as controls. .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA).

    Genome Wide:

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA). .. Refer to Kampmann et al for additional information regarding genome-wide screening techniques utilizing shRNA libraries.

    Acrylamide Gel Assay:

    Article Title: Primate piRNA Cluster Evolution Suggests Limited Relevance of Pseudogenes in piRNA-Mediated Gene Regulation
    Article Snippet: .. Isolated RNA was applied to a 12% urea-based denaturing acrylamide gel and run for 20 min at 1,200 V and 50 mA (60W) together with a 31-mer internal RNA marker piSPIKETM (IDT) and GeneRuler Ultra Low Range DNA ladder (ThermoScientific). .. The small RNA fraction ranging from 20 to 35 nt was excised from the gel and dissolved in H2 O with the Ultrafree-MC system (Millipore).

    Hybridization:

    Article Title: Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum
    Article Snippet: The membranes were reprobed with 32 P-end-labeled oligonucleotides 1121 and 885 for mir-1185-3p and mir-1183-5p as previously described whereafter hybridization was analyzed by PhospImager (Bio-Rad). .. GeneRule Ultra Low Range DNA Ladder (ThermoFisher Scientific) was labeled the same way as labeling oligo 102 and used as size standard.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: Paragraph title: Hybridization ... We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments.

    Chromatography:

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: .. We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments. .. Electrophoresis was carried out at a constant voltage of 120 V for 90 min.

    Ancient DNA Assay:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: Libraries were then aligned to appropriate reference genomes (cow, dog, dolphin, elephant, horse, human (hg19), polar bear and rhinoceros) using BWA with ancient DNA parameters ). .. In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns.

    Northern Blot:

    Article Title: Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum
    Article Snippet: Paragraph title: Northern blot ... GeneRule Ultra Low Range DNA Ladder (ThermoFisher Scientific) was labeled the same way as labeling oligo 102 and used as size standard.

    Infection:

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: K562R or K562S cells were then infected with virus at a multiplicity of infection of 1, followed by spinoculation; see supplemental Methods. .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA).

    Silica Based DNA Purification:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: .. In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Following separation of DNA extracts on a 4% agarose gel, we confirmed that DNA fragments ≥ 35 bp were efficiently retrieved from all buffers, while DNA fragments between 20–25 bp were also retrieved from water, TET, monosodium phosphate and sodium acetate, pointing to a better recovery of short fragments from low-salt or acidic solutions (Supplementary Fig. ).

    Generated:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: To quantify the amount of DNA polymer synthesized in a given channel, the RCA product generated from Circle2 on a non-patterned surface was digested in situ using a restriction enzyme which cuts only once in each repeat unit of the RCA product. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Imaging:

    Article Title: Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum
    Article Snippet: Subsequently, membranes were washed and hybridization signals were analyzed by ChemiDoc Imaging System (Bio-Rad) according to the DIG System and the DIG Application Manual (Merck). .. GeneRule Ultra Low Range DNA Ladder (ThermoFisher Scientific) was labeled the same way as labeling oligo 102 and used as size standard.

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The gel was stained with SYBR Gold (Cat. # S11494, Invitrogen) and imaged on a Gel Doc imaging system and analyzed using Discovery One software (Bio-Rad Laboratories). .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Sequencing:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: Paragraph title: Pretreatment, DNA extraction, library preparation and sequencing ... In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns.

    Article Title: Primate piRNA Cluster Evolution Suggests Limited Relevance of Pseudogenes in piRNA-Mediated Gene Regulation
    Article Snippet: Paragraph title: Small RNA Library Preparation and Sequencing ... Isolated RNA was applied to a 12% urea-based denaturing acrylamide gel and run for 20 min at 1,200 V and 50 mA (60W) together with a 31-mer internal RNA marker piSPIKETM (IDT) and GeneRuler Ultra Low Range DNA ladder (ThermoScientific).

    Binding Assay:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: .. In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Following separation of DNA extracts on a 4% agarose gel, we confirmed that DNA fragments ≥ 35 bp were efficiently retrieved from all buffers, while DNA fragments between 20–25 bp were also retrieved from water, TET, monosodium phosphate and sodium acetate, pointing to a better recovery of short fragments from low-salt or acidic solutions (Supplementary Fig. ).

    Molecular Weight:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker. .. To approximate the average polymer length and rate of DNA synthesis, the individual RCA products were cut and released from the surface of a non-patterned substrate using a restriction enzyme that cuts only at the base of each polymer.

    DNA Extraction:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: Paragraph title: Pretreatment, DNA extraction, library preparation and sequencing ... In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns.

    Nucleic Acid Electrophoresis:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: Paragraph title: Characterization by Gel Electrophoresis ... An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Isolation:

    Article Title: Primate piRNA Cluster Evolution Suggests Limited Relevance of Pseudogenes in piRNA-Mediated Gene Regulation
    Article Snippet: .. Isolated RNA was applied to a 12% urea-based denaturing acrylamide gel and run for 20 min at 1,200 V and 50 mA (60W) together with a 31-mer internal RNA marker piSPIKETM (IDT) and GeneRuler Ultra Low Range DNA ladder (ThermoScientific). .. The small RNA fraction ranging from 20 to 35 nt was excised from the gel and dissolved in H2 O with the Ultrafree-MC system (Millipore).

    Labeling:

    Article Title: Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum
    Article Snippet: .. GeneRule Ultra Low Range DNA Ladder (ThermoFisher Scientific) was labeled the same way as labeling oligo 102 and used as size standard. ..

    Purification:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: .. In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Following separation of DNA extracts on a 4% agarose gel, we confirmed that DNA fragments ≥ 35 bp were efficiently retrieved from all buffers, while DNA fragments between 20–25 bp were also retrieved from water, TET, monosodium phosphate and sodium acetate, pointing to a better recovery of short fragments from low-salt or acidic solutions (Supplementary Fig. ).

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA). .. Polymerase chain reaction (PCR) products were sequenced to high depth (Illumina HiSequation 2000).

    Polymerase Chain Reaction:

    Article Title: Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus
    Article Snippet: Paragraph title: PCR analysis ... Lanes were loaded with either GeneRuler Ultra Low Range DNA Ladder (Thermo Fisher Scientific), or the samples.

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA). .. Polymerase chain reaction (PCR) products were sequenced to high depth (Illumina HiSequation 2000).

    Article Title: Isolation and characterization of genomic microsatellite markers for small cardamom (Elettaria cardamomum Maton) for utility in genetic diversity analysis
    Article Snippet: Amplification of microsatellite loci was carried out in a 25 µl PCR reaction mixture containing, 1 × PCR buffer, 2.0 mM Mgcl2 (GeNeiTM, Bangalore), 0.1 mM dNTPs (Thermoscientific, USA), 5.0 pmol each of primers, 40–50 ng of genomic DNA and 0.5 U Taq DNA polymerase (GeNeiTM, Bangalore) in an Eppendorf thermal cycler (Master cycler EP Gradient S, Eppendorf, Germany) with the following amplification profile: initial denaturation at 94 °C for 30 s, annealing at 60–61 °C for 45 s, (depending up on primers used, see Table 2, Online Resource 1) extension at 72 °C for 1 min, final extension at 72 °C for 10 min and a hold at 4 °C. .. Amplified products were resolved in a 15 % denaturing polyacrylamide gel along with GeneRuler™100 bp and GeneRuler™ ultra low range DNA ladder (Thermoscientific, USA).

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays
    Article Snippet: Fermentas 6 x Orange DNA Loading Dye® (1 μL) (Fermentas, Lithuania) was mixed with 1 μL of 100 X GelStar® (Lonza, USA) and 1 μL of Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder [this consisted of 10 mM Tris–HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF and 10% glycerol (Fermentas, Lithuania)] or DNA sample on Parafilm® (Parafilm, USA) before adding into the well. .. For PCR and PCR-RFLP products, 4 μL of each product was mixed with 1 μL of Fermentas 6 x Orange DNA Loading Dye® [10 mM Tris–HCl (pH 7.6), 0.15% of Orange G, 0.03% of xylene cyanol FF, 60% glycerol and 60 mM EDTA (Fermentas, Lithuania)] and 1 μL of 100 X GelStar® (Lonza, USA).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The channels were washed briefly with a reaction buffer (0.050 m potassium acetate, 0.020 m Tris acetate, 0.01 m magnesium acetate) and an enzyme solution containing 1 U · μL−1 BanI (Cat. # R0118L, New England Biolabs) in 0.050 m potassium acetate, 0.020 m Tris acetate, 0.010 m magnesium acetate, 100 μg · mL−1 BSA, and 10−3 m dithiothreitol, pH = 7.9 was introduced into the chamber and the chip was incubated at 37 °C for ≈12 h. The digestion solution was collected from the chamber and analyzed using polyacrylamide gel electrophoresis. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: The formation of double stranded miRNA-145 was analysed by 15% polyacrylamide gel electrophoresis with 1 × TAE buffer. .. We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments.

    Article Title: A DNA nanoscope via auto-cycling proximity recording
    Article Snippet: Electrophoresis Gels (8 × 8 cm) for denaturing polyacrylamide gel electrophoresis (PAGE) were cast in house at 8% acrylamide (J.T.Baker), with 7 M urea (Sigma) and 1× TAE (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) buffer (GrowCells.com), in plastic cassettes (Life Technologies), and run for 30–35 min at 200 V and 65 °C in 1× TAE buffer. .. All ladders shown are 10–300 nt (Thermo Scientific, Cat. No. SM1213).

    Lysis:

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: .. In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Following separation of DNA extracts on a 4% agarose gel, we confirmed that DNA fragments ≥ 35 bp were efficiently retrieved from all buffers, while DNA fragments between 20–25 bp were also retrieved from water, TET, monosodium phosphate and sodium acetate, pointing to a better recovery of short fragments from low-salt or acidic solutions (Supplementary Fig. ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: First, a complimentary oligonucleotide, 5′-CGA CGT ATA TGA TGG TAC CGC AGC CAG CAT CAC CAG A-3′, was introduced at a concentration of 5.0 × 10−6 m in a 2X SSC solution and hybridized to the RCA product using the protocol described above. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Chromatin Immunoprecipitation:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The channels were washed briefly with a reaction buffer (0.050 m potassium acetate, 0.020 m Tris acetate, 0.01 m magnesium acetate) and an enzyme solution containing 1 U · μL−1 BanI (Cat. # R0118L, New England Biolabs) in 0.050 m potassium acetate, 0.020 m Tris acetate, 0.010 m magnesium acetate, 100 μg · mL−1 BSA, and 10−3 m dithiothreitol, pH = 7.9 was introduced into the chamber and the chip was incubated at 37 °C for ≈12 h. The digestion solution was collected from the chamber and analyzed using polyacrylamide gel electrophoresis. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Software:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The gel was stained with SYBR Gold (Cat. # S11494, Invitrogen) and imaged on a Gel Doc imaging system and analyzed using Discovery One software (Bio-Rad Laboratories). .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    shRNA:

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: Paragraph title: shRNA library screen ... Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA).

    Agarose Gel Electrophoresis:

    Article Title: Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
    Article Snippet: Paragraph title: Agarose gel analysis ... 5 µL of dsDNA mixed with 1 µL 6x DNA Loading Dye (Thermo Scientific) and 4 µL of GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific) were loaded on the gel and kept running for 15 min at 150 V. Visualization of the DNA was performed with a Genoplex system (VWR).

    Article Title: A combined method for DNA analysis and radiocarbon dating from a single sample
    Article Snippet: In order to evaluate the suitability of our silica-based DNA purification protocol for DNA retrieval from all buffers used in this study, a mixture containing 1 μg (2 μL) of Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, 500 µL of guanidine hydrochloride binding buffer and 20 µL of 3 M sodium acetate was added to 100 µL of either water, TET buffer (10 mM Tris-HCl, 1 mM EDTA, 0.05% Tween-20), 0.5 M EDTA pH 8.0, 0.5 M sodium phosphate pH 7.0, 0.5 M monosodium phosphate pH 3.0–4.5, lysis buffer, or 0.5 M sodium acetate pH 5.2, and purified using MinElute silica columns. .. Following separation of DNA extracts on a 4% agarose gel, we confirmed that DNA fragments ≥ 35 bp were efficiently retrieved from all buffers, while DNA fragments between 20–25 bp were also retrieved from water, TET, monosodium phosphate and sodium acetate, pointing to a better recovery of short fragments from low-salt or acidic solutions (Supplementary Fig. ).

    Article Title: shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance
    Article Snippet: .. Amplicons sizes were checked on a 3% agarose gel using GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific, Waltham, MA), then purified (PCR Clean-Up Kit, Qiagen, Valencia, CA). .. Polymerase chain reaction (PCR) products were sequenced to high depth (Illumina HiSequation 2000).

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays
    Article Snippet: Paragraph title: Agarose gel electrophoresis ... Fermentas 6 x Orange DNA Loading Dye® (1 μL) (Fermentas, Lithuania) was mixed with 1 μL of 100 X GelStar® (Lonza, USA) and 1 μL of Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder [this consisted of 10 mM Tris–HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF and 10% glycerol (Fermentas, Lithuania)] or DNA sample on Parafilm® (Parafilm, USA) before adding into the well.

    In Situ:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: To quantify the amount of DNA polymer synthesized in a given channel, the RCA product generated from Circle2 on a non-patterned surface was digested in situ using a restriction enzyme which cuts only once in each repeat unit of the RCA product. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Concentration Assay:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: First, a complimentary oligonucleotide, 5′-CGA CGT ATA TGA TGG TAC CGC AGC CAG CAT CAC CAG A-3′, was introduced at a concentration of 5.0 × 10−6 m in a 2X SSC solution and hybridized to the RCA product using the protocol described above. .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: They were dissolved in RNase-free water to produce solutions of equal concentration (50 μM), mixed and heated to 90 °C for 4 min to remove secondary structures. .. We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments.

    Marker:

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker. .. To approximate the average polymer length and rate of DNA synthesis, the individual RCA products were cut and released from the surface of a non-patterned substrate using a restriction enzyme that cuts only at the base of each polymer.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: .. We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments. .. Electrophoresis was carried out at a constant voltage of 120 V for 90 min.

    Staining:

    Article Title: Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus
    Article Snippet: Lanes were loaded with either GeneRuler Ultra Low Range DNA Ladder (Thermo Fisher Scientific), or the samples. .. Staining was performed with GelRed (Biotium).

    Article Title: Isolation and characterization of genomic microsatellite markers for small cardamom (Elettaria cardamomum Maton) for utility in genetic diversity analysis
    Article Snippet: Amplified products were resolved in a 15 % denaturing polyacrylamide gel along with GeneRuler™100 bp and GeneRuler™ ultra low range DNA ladder (Thermoscientific, USA). .. The gels were silver stained (Bassam and Gresshoff ) to view the bands and images were saved by scanning the gels.

    Article Title: Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
    Article Snippet: The gel was stained with SYBR Gold (Cat. # S11494, Invitrogen) and imaged on a Gel Doc imaging system and analyzed using Discovery One software (Bio-Rad Laboratories). .. An ultralow-range gene ruler (Cat. # SM1211, Fermentas Life Sciences) was used as a molecular-weight marker.

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
    Article Snippet: We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments. .. The gel was stained with SYBRGold (Life Technologies, Carlsbad, CA, USA).

    Article Title: A DNA nanoscope via auto-cycling proximity recording
    Article Snippet: Gels were then removed from cassettes, stained in 1× SybrGold (Life Technologies) for 15 min, and imaged with a Typhoon scanner (General Electric). .. All ladders shown are 10–300 nt (Thermo Scientific, Cat. No. SM1213).

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    Thermo Fisher generuler ultra low range dna ladder
    Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. <t>Generuler</t> ultra low range <t>DNA</t> ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.
    Generuler Ultra Low Range Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    generuler ultra low range dna ladder - by Bioz Stars, 2020-04
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    Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. Generuler ultra low range DNA ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.

    Journal: Scientific Reports

    Article Title: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

    doi: 10.1038/srep13567

    Figure Lengend Snippet: Polyacrylamide gel electrophoresis (15%) using 1x TAE buffer (constant voltage of 120 V, 90 min) and stained with SYBR gold (1x). Lane codes: M. Generuler ultra low range DNA ladder; 1. hsa-miR-145-3p; 2. hsa-miR-145-5p; 3. miRNA-145.

    Article Snippet: We used as a marker a Generuler ultra low range DNA ladder (Thermo Fischer Scientific Inc., Waltham, USA) that contains 11 individual chromatography-purified DNA fragments.

    Techniques: Polyacrylamide Gel Electrophoresis, Staining