generead library quant kit  (Qiagen)


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    Structured Review

    Qiagen generead library quant kit
    Generead Library Quant Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead library quant kit/product/Qiagen
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    generead library quant kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: Briefly, the V3-V4 region of the bacterial 16S rRNA gene was amplified in triplicate by PCR using the TaKaRa Ex Taq HS Kit (TaKaRa Bio, Shiga, Japan) with the following program: preheating at 94°C for 3 min; 30 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 30 s and extension at 72°C for 30 s; and a terminal extension at 72°C for 5 min. A 1 μl sample of the combined PCR products was amplified with the barcoded primers adapted for Illumina MiSeq sequencing. .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol.

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: Briefly, the V3-V4 region of the bacterial 16S rRNA gene was amplified by PCR in triplicate using the TaKaRa Ex Taq HS Kit (TaKaRa Bio, Shiga, Japan) and the primer sets Tru357F (5′-CGCTCTTCCGATCTCTGTACGGRAGGCAGCA G-3′) and Tru806R (5′-CGCTCTTCCGATCTGACG- GACTACHVGGGTWTCTAAT-3′) with the following program: preheating at 94°C for 3 min; 30 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 30 s and extension at 72°C for 30 s; and terminal extension at 72°C for 5 min. A 1-μl sample of the combined PCR products was amplified with barcoded primers adapted for Illumina MiSeq sequencing: Fwd 5′-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCTCTG-3’ and Rev 5′-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGAC-3′, where X represents a barcode base. .. Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol.

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: The products were given d(A) tails and ligated with the second adapter using the same reagents as in on-bead reactions and then amplified by PCR for 20 to 22 cycles using Phusion high-fidelity DNA polymerase (New England Biolabs). .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). .. Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina) following the manufacturer's instructions.

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: Entire and potentially active archaeal communities were assessed by Illumina tag sequencing of 16S rRNA amplicons generated from 16S rRNA genes and transcripts amplified by PCR and RT-PCR, respectively. .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Adapter ligation, further Ampure purification, and library preparation were completed by 14 cycles of PCR amplification. .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C. .. The USER-treated DNA was amplified by PCR to generate sequencing library for Illumina sequencing.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio. .. The final sample underwent paired-end sequencing using an Illumina MiSeq instrument, according to the manufacturer’s protocol.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: After validating the 2nd amplified DNA product using the QIAxcel system, the PCR products were purified using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol. .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    Whole Genome Amplification:

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: Sequencing For DNA fragmentation, we used 2 μg genomic DNA or WGA DNA (without subsequent purification of WGA DNA) and the Covaris® S220 instrument. .. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook.

    Synthesized:

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The second strand was synthesized from this cDNA with 20 μl of fragmented cDNA:RNA, 4 μl of 5× first strand buffer, 30 μl of 5× second strand buffer, 4 μl of 10 mM dNTP with dUTP instead of dTTP, 2 μl of 100 mM DTT, 4 μl of E. coli DNA polymerase (Invitrogen), 1 μl of E. coli DNA ligase (Invitrogen), 1 μl of E. coli RNase H (Invitrogen) in 150 μl of total volume. .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Picogreen Assay:

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: The products were purified and quantified by a QIAquick PCR Purification Kit (Qiagen, Valencia, CA, United States) and Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s protocols. .. Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: The purified products were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA). .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    Incubation:

    Article Title: Draft Genome Sequences of Two Staphylococcus aureus Strains Isolated in Succession from a Case of Bacteremia
    Article Snippet: Cell suspensions were further incubated with 30 µL of 10% SDS and 10 µL of proteinase K (20 mg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) for 30 min. Five molar NaCl (100 µL) was added, vortexed before the addition of 80 µL of cetyltrimethylammonium bromide (10% wt/vol)–NaCl solution (4.1% wt/vol) (Sigma-Aldrich, St. Louis, MO, USA), and incubated at 65°C (10 min). .. Libraries were pooled and purified with a GeneRead size-selection kit (Qiagen, Valencia, CA, USA); the pooled DNA libraries were quantified with a Qubit dsDNA high-sensitivity assay kit (Thermo, Fisher Scientific) and analyzed with a high-sensitivity DNA chip (Agilent, Santa Clara, CA, USA).

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: To elute and reverse cross-link the bound complexes, TE buffer containing 1% SDS was added to the beads and samples were incubated overnight at 65°C. .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The DNA samples incubated for primer extension as described previously ( ) were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: A tailing, bead-bound sample was incubated with 50 μL buffer enzyme mix at 30 °C for 30 minutes, followed by PEG/NaCl purification. .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C. .. The USER-treated DNA was amplified by PCR to generate sequencing library for Illumina sequencing.

    In Silico:

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions. .. The primer sequences were deduced from highly conserved regions and tested in silico using TestPrime ( https://www.arb-silva.de/search/testprime/ ) and the SILVA 132 SSU Ref NR 99 [ ] as reference database.

    Expressing:

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: Paragraph title: RNA-seq expression profiling ... The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Modification:

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: Microbiota Analysis DNA was extracted from the fecal samples by the bead-beating method ( ) and 16S rRNA gene sequencing was performed as previously described with minor modification ( ). .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio. .. Statistical analysis and determination of modification frequency was performed using CRISPR-DAV and Cas-Analyzer .

    Transfection:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: High-throughput sequencing 100 ng gDNA isolated from cells from each transfection was amplified by PCR for 35 cycles with primers PCR1_fwd and PCR1_rev (listed in Supplementary Table ) and 2× Q5 Hot Start High Fidelity Master Mix according to the manufacturer’s instructions (NEB). .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio.

    Ligation:

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR. .. The final DNA samples were measured by using a Qubit double-stranded DNA (dsDNA) high-sensitivity (HS) assay kit (Thermo Scientific) and 2200 TapeStation (Agilent Technologies), pooled in equimolar amounts, and sequenced on the NextSeq 500 system (2 × 75 bp, mid-output mode; Illumina).

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The DNA samples incubated for primer extension as described previously ( ) were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Adapter ligation, further Ampure purification, and library preparation were completed by 14 cycles of PCR amplification. .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The fragmented DNA was end-repaired with End Repair Kit (New England Biolabs), and dA-tailed with dA-Tailing Kit (New England Biolabs), and then ligated with 7.5 μg of DNA adaptor mixture with Quick Ligation Kit (New England Biolabs). .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Generated:

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: Subsequently, cDNA was generated from DNA-free RNA using the SuperScript III reverse transcriptase (Thermo Fisher Scientific) according to Wemheuer et al. [ ] using the reverse primer without MiSeq adapter (5′-CCC GCC AAT TYC TTT AAG-3′; this study). .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit. .. Generated raw reads were filtered for quality (phred33 ≥ 30) and length (n ≥ 32), and adapter sequences were removed using Trimmomatic v.0.22 [ ].

    Polymerase Chain Reaction:

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: Briefly, the V3-V4 region of the bacterial 16S rRNA gene was amplified in triplicate by PCR using the TaKaRa Ex Taq HS Kit (TaKaRa Bio, Shiga, Japan) with the following program: preheating at 94°C for 3 min; 30 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 30 s and extension at 72°C for 30 s; and a terminal extension at 72°C for 5 min. A 1 μl sample of the combined PCR products was amplified with the barcoded primers adapted for Illumina MiSeq sequencing. .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol.

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: The products were purified and quantified by a QIAquick PCR Purification Kit (Qiagen, Valencia, CA, United States) and Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s protocols. .. Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol.

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR. .. The final DNA samples were measured by using a Qubit double-stranded DNA (dsDNA) high-sensitivity (HS) assay kit (Thermo Scientific) and 2200 TapeStation (Agilent Technologies), pooled in equimolar amounts, and sequenced on the NextSeq 500 system (2 × 75 bp, mid-output mode; Illumina).

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: For RNA analysis, residual DNA was removed from extracted RNA by Turbo DNAse treatment, and the absence of DNA was confirmed by PCR using universal primers as described by Schneider et al. [ ]. .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Adapter ligation, further Ampure purification, and library preparation were completed by 14 cycles of PCR amplification. .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: This reaction mixture was incubated at 16°C for 2 h, and fragmented DNA was recovered with PCR clean-up kit (QIAGEN) and eluted in 30 μl of nuclease-free water. .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Purified PCR products were then amplified by PCR with Nextera XT Illumina primers for 7 cycles with 2× Q5 Hot Start High Fidelity Master Mix in Q5 Reaction Buffer (NEB), to add unique barcodes. .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: After validating the 2nd amplified DNA product using the QIAxcel system, the PCR products were purified using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol. .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: After fragmentation, DNA was purified using the MinElute® PCR Purification Kit (QIAGEN) according to the protocol in the kit handbook. .. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: Entire and potentially active archaeal communities were assessed by Illumina tag sequencing of 16S rRNA amplicons generated from 16S rRNA genes and transcripts amplified by PCR and RT-PCR, respectively. .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Binding Assay:

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). .. The antibody (NT63, Biolegend) that specifically recognize RNA polymerase β was used to conduct the ChIP-exo experiment to detect the binding sites of RNA polymerase in E. coli K-12 MG1655.

    ChIP-sequencing:

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Paragraph title: ChIP-Seq ... Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    DNA Extraction:

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: After protease K (Thermo Scientific) digestion and DNA extraction with phenol-chloroform-isoamyl alcohol (Amresco, USA), the single-strand DNAs were subjected to primer extension using phi29 DNA polymerase (New England Biolabs). .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR.

    RNA Sequencing Assay:

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: Paragraph title: RNA-seq expression profiling ... The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Isolation:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: High-throughput sequencing 100 ng gDNA isolated from cells from each transfection was amplified by PCR for 35 cycles with primers PCR1_fwd and PCR1_rev (listed in Supplementary Table ) and 2× Q5 Hot Start High Fidelity Master Mix according to the manufacturer’s instructions (NEB). .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio.

    Purification:

    Article Title: Draft Genome Sequences of Two Staphylococcus aureus Strains Isolated in Succession from a Case of Bacteremia
    Article Snippet: .. Libraries were pooled and purified with a GeneRead size-selection kit (Qiagen, Valencia, CA, USA); the pooled DNA libraries were quantified with a Qubit dsDNA high-sensitivity assay kit (Thermo, Fisher Scientific) and analyzed with a high-sensitivity DNA chip (Agilent, Santa Clara, CA, USA). .. Libraries were paired-end (76 × 2) sequenced in a single dual-indexed (8 × 2) paired-end (76 × 2) NextSeq500 using a Mid Output (150 cycle) version 2 kit (Illumina) as recommended by the manufacturer.

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol. .. The pooled libraries were sequenced by an Illumina MiSeq instrument and the MiSeq v3 Reagent Kit (Illumina Inc, San Diego, CA, United States).

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: .. Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol. .. The pooled libraries were sequenced with an Illumina MiSeq instrument and the MiSeq v3 Reagent Kit (Illumina Inc., San Diego, CA, United States).).

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). .. Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina) following the manufacturer's instructions.

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions. .. Archaeal 16S rRNA genes were amplified using the forward primer Arch514Fa (5′-GGT GBC AGC CGC CGC GGT AA-3′; [ ]) and the reverse primer (5′-CCC GCC AAT TYC TTT AAG-3′; this study) with Illumina Nextera adapters attached for sequencing.

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit. .. ChIP libraries were sequenced at McGill using Illumina HiSeq 2000 with 100-bp single-ended reads.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio. .. The final sample underwent paired-end sequencing using an Illumina MiSeq instrument, according to the manufacturer’s protocol.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: The purified products were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA). .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: .. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook. .. We performed 150 bp paired-end sequencing on an Illumina® MiSeq® instrument.

    Sequencing:

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: Briefly, the V3-V4 region of the bacterial 16S rRNA gene was amplified in triplicate by PCR using the TaKaRa Ex Taq HS Kit (TaKaRa Bio, Shiga, Japan) with the following program: preheating at 94°C for 3 min; 30 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 30 s and extension at 72°C for 30 s; and a terminal extension at 72°C for 5 min. A 1 μl sample of the combined PCR products was amplified with the barcoded primers adapted for Illumina MiSeq sequencing. .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol.

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: Paragraph title: Sequencing and data processing of bacterial 16S rRNA sequences ... Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol.

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR. .. ChIP-exo sequencing reads were mapped to reference genome assembly R64-2-1 of S. cerevisiae S288C with Bowtie2 ( ) using the default settings to generate Sequence Alignment/Map (SAM) files.

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: Paragraph title: 2.3. Nucleic Acid Extraction and Sequencing ... Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C. .. The USER-treated DNA was amplified by PCR to generate sequencing library for Illumina sequencing.

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Paragraph title: High-throughput sequencing ... Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: Paragraph title: Sequencing and data processing ... Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: Paragraph title: Sequencing ... The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook.

    CRISPR:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio. .. Statistical analysis and determination of modification frequency was performed using CRISPR-DAV and Cas-Analyzer .

    Chromatin Immunoprecipitation:

    Article Title: Draft Genome Sequences of Two Staphylococcus aureus Strains Isolated in Succession from a Case of Bacteremia
    Article Snippet: .. Libraries were pooled and purified with a GeneRead size-selection kit (Qiagen, Valencia, CA, USA); the pooled DNA libraries were quantified with a Qubit dsDNA high-sensitivity assay kit (Thermo, Fisher Scientific) and analyzed with a high-sensitivity DNA chip (Agilent, Santa Clara, CA, USA). .. Libraries were paired-end (76 × 2) sequenced in a single dual-indexed (8 × 2) paired-end (76 × 2) NextSeq500 using a Mid Output (150 cycle) version 2 kit (Illumina) as recommended by the manufacturer.

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: Paragraph title: ChIP-exo preparation ... The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: Paragraph title: ChIP-exo. ... The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: Paragraph title: ChIP-exo experiment ... The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: Paragraph title: ChIP-exo experiment ... The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: Briefly, 25 μl of ChIP sample was incubated with 20 μl end repair mix at 20 °C for 30 minutes followed by Ampure XP bead purification. .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit.

    Software:

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol. .. Non-chimeric sequences were analysed via the QIIME software package version 1.8.0[ , ].

    Selection:

    Article Title: Decreased Taxon-Specific IgA Response in Relation to the Changes of Gut Microbiota Composition in the Elderly
    Article Snippet: .. Equal amounts of the amplicons were pooled and purified by the GeneRead Size Selection Kit (Qiagen, Valencia, CA, United States) according to the manufacturer’s protocol. .. The pooled libraries were sequenced by an Illumina MiSeq instrument and the MiSeq v3 Reagent Kit (Illumina Inc, San Diego, CA, United States).

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
    Article Snippet: .. Equal amounts of amplicons were pooled and purified with the GeneRead Size Selection Kit (Qiagen) according to the manufacturer’s protocol. .. The pooled libraries were sequenced with an Illumina MiSeq instrument and the MiSeq v3 Reagent Kit (Illumina Inc., San Diego, CA, United States).).

    Article Title: Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae
    Article Snippet: .. The GeneRead size selection kit (Qiagen) was used to purify DNA before and after the second incorporation of d(A) tails, second adapter ligation, and PCR. .. The final DNA samples were measured by using a Qubit double-stranded DNA (dsDNA) high-sensitivity (HS) assay kit (Thermo Scientific) and 2200 TapeStation (Agilent Technologies), pooled in equimolar amounts, and sequenced on the NextSeq 500 system (2 × 75 bp, mid-output mode; Illumina).

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). .. Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina) following the manufacturer's instructions.

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions. .. Archaeal 16S rRNA genes were amplified using the forward primer Arch514Fa (5′-GGT GBC AGC CGC CGC GGT AA-3′; [ ]) and the reverse primer (5′-CCC GCC AAT TYC TTT AAG-3′; this study) with Illumina Nextera adapters attached for sequencing.

    Article Title: Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
    Article Snippet: .. Size selection was performed using a Sage Pippin prep system and set to collect 200–400-bp fragments, targeting a 300-bp peak fragment size and final libraries were purified with Qiagen GeneRead Size Selection kit. .. ChIP libraries were sequenced at McGill using Illumina HiSeq 2000 with 100-bp single-ended reads.

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C. .. The USER-treated DNA was amplified by PCR to generate sequencing library for Illumina sequencing.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: .. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies).

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: .. Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio. .. The final sample underwent paired-end sequencing using an Illumina MiSeq instrument, according to the manufacturer’s protocol.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA). .. The pooled libraries were sequenced using an Illumina MiSeq instrument with a MiSeq v3 Reagent Kit (Illumina, Inc., San Diego, CA, USA).

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: .. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook. .. We performed 150 bp paired-end sequencing on an Illumina® MiSeq® instrument.

    Ethanol Precipitation:

    Article Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP
    Article Snippet: The cDNA was recovered by phenol–chloroform extraction followed by ethanol precipitation. .. The adaptor-ligated DNA was size-selected to removed un-ligated adaptors with GeneRead Size Selection Kit (QIAGEN), and treated with 1 U of USER enzyme (New England Biolabs) in 30 μl of total volume, and incubated at 37 °C for 15 min followed by 5 min at 95°C.

    Next-Generation Sequencing:

    Article Title: Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
    Article Snippet: .. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook. .. We performed 150 bp paired-end sequencing on an Illumina® MiSeq® instrument.

    Concentration Assay:

    Article Title: Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean
    Article Snippet: The concentration and purity of DNA and RNA extracts were determined spectrophotometrically (NanoDrop 2000c; Thermo Fisher Scientific, USA). .. Extracted DNA was treated with RNAse A as described by Schneider et al. [ ] and purified using the GeneRead Size Selection kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study
    Article Snippet: Each 1-μl sample of DNA, at a concentration of approximately 10–200 ng/μl as measured using a Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA), was amplified in triplicate using the following protocol: preheating at 94 °C for 3 min; 20 cycles of denaturation at 94 °C for 30 s, annealing at 50 °C for 30 s and extension at 72 °C for 30 s; and a final terminal extension at 72 °C for 10 min. After verifying the amplified DNA based on PCR product size using the QIAxcel system (Qiagen, Valencia, CA, USA), the triplicate samples were combined. .. Subsequently, equal amounts of the amplicons from multiple samples were pooled, and primer dimers were removed using a GeneRead Size Selection Kit (Qiagen, Valencia, CA, USA).

    High Throughput Screening Assay:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: Paragraph title: High-throughput sequencing ... Amplified control and treated DNA pools were subsequently purified with the GeneRead Size Selection Kit (Qiagen), quantified using the Qubit 2.0 Fluorometer (ThermoFisher), and pooled in a 1:1 ratio.

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    Qiagen generead library quant kit
    Generead Library Quant Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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