genelute polymerase chain reaction pcr clean up kit  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    GenElute PCR Clean Up Kit
    Description:
    The GenElute PCR Clean Up Kit is designed for rapid purification of single stranded or double stranded PCR amplification products 100 bp to 10 kb from other components in the reaction such as excess primers nucleotides DNA polymerase oil and salts This kit combines the advantages of silica binding with a convenient spin column format eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform
    Catalog Number:
    na1020
    Price:
    None
    Applications:
    GenElute(TM) PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
    Buy from Supplier


    Structured Review

    Millipore genelute polymerase chain reaction pcr clean up kit
    GenElute PCR Clean Up Kit
    The GenElute PCR Clean Up Kit is designed for rapid purification of single stranded or double stranded PCR amplification products 100 bp to 10 kb from other components in the reaction such as excess primers nucleotides DNA polymerase oil and salts This kit combines the advantages of silica binding with a convenient spin column format eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform
    https://www.bioz.com/result/genelute polymerase chain reaction pcr clean up kit/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    genelute polymerase chain reaction pcr clean up kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation
    Article Snippet: .. Pooled PCR products were purified using the GenElute™ PCR Clean-Up Kit (Sigma Life Science, St. Louis, MO, USA) and cloned using the TOPO TA Cloning® kit (Invitrogen, Carlsbad, CA, USA) in combination with chemically competent Escherichia coli (TOP10 cells, Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. The clone PCR mixture contained the same reagents and concentrations as described above for the EM.

    Amplification:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: POTENTIAL EPIGENETIC DYSREGULATION OF GENES ASSOCIATED WITH MODY AND TYPE 2 DIABETES IN HUMANS EXPOSED TO A DIABETIC INTRAUTERINE ENVIRONMENT: AN ANALYSIS OF GENOME-WIDE DNA METHYLATION
    Article Snippet: .. DNA was amplified and cleaned using the GenomePlex Complete Whole Genome Amplification Kit and GenElute PCR Clean-Up Kit (Sigma). .. For microarray experiments we interrogated IP and Input DNA against the Genechip Tiling 2.0R Array, on a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Whole Genome Amplification:

    Article Title: POTENTIAL EPIGENETIC DYSREGULATION OF GENES ASSOCIATED WITH MODY AND TYPE 2 DIABETES IN HUMANS EXPOSED TO A DIABETIC INTRAUTERINE ENVIRONMENT: AN ANALYSIS OF GENOME-WIDE DNA METHYLATION
    Article Snippet: .. DNA was amplified and cleaned using the GenomePlex Complete Whole Genome Amplification Kit and GenElute PCR Clean-Up Kit (Sigma). .. For microarray experiments we interrogated IP and Input DNA against the Genechip Tiling 2.0R Array, on a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA).

    TA Cloning:

    Article Title: Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation
    Article Snippet: .. Pooled PCR products were purified using the GenElute™ PCR Clean-Up Kit (Sigma Life Science, St. Louis, MO, USA) and cloned using the TOPO TA Cloning® kit (Invitrogen, Carlsbad, CA, USA) in combination with chemically competent Escherichia coli (TOP10 cells, Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. The clone PCR mixture contained the same reagents and concentrations as described above for the EM.

    Purification:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation
    Article Snippet: .. Pooled PCR products were purified using the GenElute™ PCR Clean-Up Kit (Sigma Life Science, St. Louis, MO, USA) and cloned using the TOPO TA Cloning® kit (Invitrogen, Carlsbad, CA, USA) in combination with chemically competent Escherichia coli (TOP10 cells, Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. The clone PCR mixture contained the same reagents and concentrations as described above for the EM.

    Article Title: Replicon typing of plasmids carrying blaCTX-M-1 in Enterobacteriaceae of animal, environmental and human origin
    Article Snippet: .. Amplicons were purified using the GenElute™ PCR Clean-Up (Sigma-Aldrich, Buchs, Switzerland) and custom sequenced by Microsynth (Balgach, Switzerland). .. Sequence types (STs) were assigned using the PubMLST database ( http://pubmlst.org/plasmid ).

    Article Title: Ubiquitous Presence and Novel Diversity of Anaerobic Alkane Degraders in Cold Marine Sediments
    Article Snippet: .. Products were purified with the GenElute PCR Clean-up kit (Sigma, Copenhagen, Denmark), and separated on a 1.5% agrose gel (120V, > 60 min). .. Bands of the correct size (∼830 nucleotides) were excised and cleaned with the GenElute Gel Extraction Kit (Sigma).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Article Title: A horizontally gene transferred copper resistance locus confers hyper‐resistance to antibacterial copper toxicity and enables survival of community acquired methicillin resistant Staphylococcus aureus USA300 in macrophages
    Article Snippet: .. These (and empty pGEM‐T for the control DNA) were used in PCRs using M13 primers to produce DNA samples for each operator/promoter region, which were purified (PCR clean‐up kit; Sigma). .. DTT was removed from recombinant CsoR in an anaerobic ([O2 ] < 5 ppm) chamber (Belle Technology) by binding to a 1 ml AEX column and washing with 10 CV anaerobic 20 mM Hepes, pH 7.5, then eluting in this buffer with 1 M NaCl.

    Polymerase Chain Reaction:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation
    Article Snippet: .. Pooled PCR products were purified using the GenElute™ PCR Clean-Up Kit (Sigma Life Science, St. Louis, MO, USA) and cloned using the TOPO TA Cloning® kit (Invitrogen, Carlsbad, CA, USA) in combination with chemically competent Escherichia coli (TOP10 cells, Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. The clone PCR mixture contained the same reagents and concentrations as described above for the EM.

    Article Title: Replicon typing of plasmids carrying blaCTX-M-1 in Enterobacteriaceae of animal, environmental and human origin
    Article Snippet: .. Amplicons were purified using the GenElute™ PCR Clean-Up (Sigma-Aldrich, Buchs, Switzerland) and custom sequenced by Microsynth (Balgach, Switzerland). .. Sequence types (STs) were assigned using the PubMLST database ( http://pubmlst.org/plasmid ).

    Article Title: POTENTIAL EPIGENETIC DYSREGULATION OF GENES ASSOCIATED WITH MODY AND TYPE 2 DIABETES IN HUMANS EXPOSED TO A DIABETIC INTRAUTERINE ENVIRONMENT: AN ANALYSIS OF GENOME-WIDE DNA METHYLATION
    Article Snippet: .. DNA was amplified and cleaned using the GenomePlex Complete Whole Genome Amplification Kit and GenElute PCR Clean-Up Kit (Sigma). .. For microarray experiments we interrogated IP and Input DNA against the Genechip Tiling 2.0R Array, on a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA).

    Article Title: Ubiquitous Presence and Novel Diversity of Anaerobic Alkane Degraders in Cold Marine Sediments
    Article Snippet: .. Products were purified with the GenElute PCR Clean-up kit (Sigma, Copenhagen, Denmark), and separated on a 1.5% agrose gel (120V, > 60 min). .. Bands of the correct size (∼830 nucleotides) were excised and cleaned with the GenElute Gel Extraction Kit (Sigma).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Article Title: A horizontally gene transferred copper resistance locus confers hyper‐resistance to antibacterial copper toxicity and enables survival of community acquired methicillin resistant Staphylococcus aureus USA300 in macrophages
    Article Snippet: .. These (and empty pGEM‐T for the control DNA) were used in PCRs using M13 primers to produce DNA samples for each operator/promoter region, which were purified (PCR clean‐up kit; Sigma). .. DTT was removed from recombinant CsoR in an anaerobic ([O2 ] < 5 ppm) chamber (Belle Technology) by binding to a 1 ml AEX column and washing with 10 CV anaerobic 20 mM Hepes, pH 7.5, then eluting in this buffer with 1 M NaCl.

    Article Title: Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP
    Article Snippet: .. The pBAD was opened by Nco I and Bgl II in restriction buffer NEB3.1 (NEB) and was subsequently column cleaned (Genelute PCR Clean-Up Kit, Sigma-Aldrich). .. RyhP-DNA fragments and pBAD were ligated (T4 ligase, NEB) and transformed in E. coli TOP10.

    Plasmid Preparation:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore multiscreen pcr clean up filter plates
    Multiscreen Pcr Clean Up Filter Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiscreen pcr clean up filter plates/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    multiscreen pcr clean up filter plates - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore genelute pcr clean up kit
    Genelute Pcr Clean Up Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genelute pcr clean up kit/product/Millipore
    Average 99 stars, based on 422 article reviews
    Price from $9.99 to $1999.99
    genelute pcr clean up kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results