genelute pcr clean up kit  (Millipore)


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    Name:
    GenElute PCR Clean Up Kit
    Description:
    The GenElute PCR Clean Up Kit is designed for rapid purification of single stranded or double stranded PCR amplification products 100 bp to 10 kb from other components in the reaction such as excess primers nucleotides DNA polymerase oil and salts This kit combines the advantages of silica binding with a convenient spin column format eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform
    Catalog Number:
    na1020
    Price:
    None
    Applications:
    GenElute(TM) PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
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    Structured Review

    Millipore genelute pcr clean up kit
    GenElute PCR Clean Up Kit
    The GenElute PCR Clean Up Kit is designed for rapid purification of single stranded or double stranded PCR amplification products 100 bp to 10 kb from other components in the reaction such as excess primers nucleotides DNA polymerase oil and salts This kit combines the advantages of silica binding with a convenient spin column format eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform
    https://www.bioz.com/result/genelute pcr clean up kit/product/Millipore
    Average 99 stars, based on 396 article reviews
    Price from $9.99 to $1999.99
    genelute pcr clean up kit - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Amplification:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Nucleic Acid Electrophoresis:

    Article Title: Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks Candida albicans Morphogenesis, and Is Effluxed via Yor1 and Circuitry Controlled by Zcf29
    Article Snippet: .. The cycling conditions were 94°C for 5 min, 94°C for 30 s, 55°C for 45 s, and 68°C for 1 min for 30 cycles, and 68°C for 10 min. PCR products were visually confirmed by gel electrophoresis and purified using a PCR cleanup kit (Sigma). .. Amounts of ∼100 ng of purified products with 7.14 μM appropriate oligonucleotides were sent for sequencing at the TCAG sequencing facility.

    Purification:

    Article Title: Small Deletion Variants Have Stable Breakpoints Commonly Associated with Alu Elements
    Article Snippet: .. Products were purified using the GenEluteTM PCR Clean-Up Kit (Sigma-Aldrich), which uses a series of washes and spins to purify the DNA. .. The concentration of each purified PCR product was then quantitated using a Nanodrop ND-1000 spectrophotometer, to calculate the amount of PCR product required for sequencing.

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Microbiological characteristics of smoked and smoked–dried fish processed in Benin, et al. Microbiological characteristics of smoked and smoked–dried fish processed in Benin
    Article Snippet: .. PCR products were purified using purification kit (GenElute™ PCR Clean‐Up; Sigma‐Aldrich). .. Identification of the bacteria was done by comparing their 16S rRNA sequence with those in databases using www.ncbi.nlm.nih.gov/BLAST .

    Article Title: An Evaluation of the Stemness, Paracrine, and Tumorigenic Characteristics of Highly Expanded, Minimally Passaged Adipose-Derived Stem Cells
    Article Snippet: .. The rest of the PCR products were purified using the GenEluteTM PCR Clean-Up Kit (Sigma-Aldrich, St. Louis, MO, USA). .. Sequencing reactions were run on an ABI3500 Genetic Analyzer sequencing system (Applied Biosystems, FosterCity, CA, USA).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Article Title: A horizontally gene transferred copper resistance locus confers hyper‐resistance to antibacterial copper toxicity and enables survival of community acquired methicillin resistant Staphylococcus aureus USA300 in macrophages
    Article Snippet: .. These (and empty pGEM‐T for the control DNA) were used in PCRs using M13 primers to produce DNA samples for each operator/promoter region, which were purified (PCR clean‐up kit; Sigma). .. DTT was removed from recombinant CsoR in an anaerobic ([O2 ] < 5 ppm) chamber (Belle Technology) by binding to a 1 ml AEX column and washing with 10 CV anaerobic 20 mM Hepes, pH 7.5, then eluting in this buffer with 1 M NaCl.

    Article Title: Identification and sequence analyses of novel lipase encoding novel thermophillic bacilli isolated from Armenian geothermal springs
    Article Snippet: .. PCR products were purified with GenElute™ PCR Clean-up Kit (Sigma). .. PCR amplification of lipase encoding genes Isolated genomic DNA was used as a template amplification of lipase encoding genes.

    Article Title: Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks Candida albicans Morphogenesis, and Is Effluxed via Yor1 and Circuitry Controlled by Zcf29
    Article Snippet: .. The cycling conditions were 94°C for 5 min, 94°C for 30 s, 55°C for 45 s, and 68°C for 1 min for 30 cycles, and 68°C for 10 min. PCR products were visually confirmed by gel electrophoresis and purified using a PCR cleanup kit (Sigma). .. Amounts of ∼100 ng of purified products with 7.14 μM appropriate oligonucleotides were sent for sequencing at the TCAG sequencing facility.

    Polymerase Chain Reaction:

    Article Title: Small Deletion Variants Have Stable Breakpoints Commonly Associated with Alu Elements
    Article Snippet: .. Products were purified using the GenEluteTM PCR Clean-Up Kit (Sigma-Aldrich), which uses a series of washes and spins to purify the DNA. .. The concentration of each purified PCR product was then quantitated using a Nanodrop ND-1000 spectrophotometer, to calculate the amount of PCR product required for sequencing.

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

    Article Title: Microbiological characteristics of smoked and smoked–dried fish processed in Benin, et al. Microbiological characteristics of smoked and smoked–dried fish processed in Benin
    Article Snippet: .. PCR products were purified using purification kit (GenElute™ PCR Clean‐Up; Sigma‐Aldrich). .. Identification of the bacteria was done by comparing their 16S rRNA sequence with those in databases using www.ncbi.nlm.nih.gov/BLAST .

    Article Title: An Evaluation of the Stemness, Paracrine, and Tumorigenic Characteristics of Highly Expanded, Minimally Passaged Adipose-Derived Stem Cells
    Article Snippet: .. The rest of the PCR products were purified using the GenEluteTM PCR Clean-Up Kit (Sigma-Aldrich, St. Louis, MO, USA). .. Sequencing reactions were run on an ABI3500 Genetic Analyzer sequencing system (Applied Biosystems, FosterCity, CA, USA).

    Article Title: Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome
    Article Snippet: .. The amplified cDNA was purified with a PCR clean-up kit (GenElute; Sigma-Aldrich). .. Quantitative real-time PCR was performed with the 7300 Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

    Article Title: A horizontally gene transferred copper resistance locus confers hyper‐resistance to antibacterial copper toxicity and enables survival of community acquired methicillin resistant Staphylococcus aureus USA300 in macrophages
    Article Snippet: .. These (and empty pGEM‐T for the control DNA) were used in PCRs using M13 primers to produce DNA samples for each operator/promoter region, which were purified (PCR clean‐up kit; Sigma). .. DTT was removed from recombinant CsoR in an anaerobic ([O2 ] < 5 ppm) chamber (Belle Technology) by binding to a 1 ml AEX column and washing with 10 CV anaerobic 20 mM Hepes, pH 7.5, then eluting in this buffer with 1 M NaCl.

    Article Title: Identification and sequence analyses of novel lipase encoding novel thermophillic bacilli isolated from Armenian geothermal springs
    Article Snippet: .. PCR products were purified with GenElute™ PCR Clean-up Kit (Sigma). .. PCR amplification of lipase encoding genes Isolated genomic DNA was used as a template amplification of lipase encoding genes.

    Article Title: Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks Candida albicans Morphogenesis, and Is Effluxed via Yor1 and Circuitry Controlled by Zcf29
    Article Snippet: .. The cycling conditions were 94°C for 5 min, 94°C for 30 s, 55°C for 45 s, and 68°C for 1 min for 30 cycles, and 68°C for 10 min. PCR products were visually confirmed by gel electrophoresis and purified using a PCR cleanup kit (Sigma). .. Amounts of ∼100 ng of purified products with 7.14 μM appropriate oligonucleotides were sent for sequencing at the TCAG sequencing facility.

    Plasmid Preparation:

    Article Title: Morpho-Typing and Molecular Diversity of Arbuscular Mycorrhizal Fungi in Sub-Tropical Soils of Coimbatore Region, Tamil Nadu, India
    Article Snippet: .. PCR amplified ITS region was purified using PCR clean-up kit (GenElute PCR Clean-up kit, Sigma, USA) according to the manufacturer’s instruction and cloned using pTZ57R/T vector supplied with T/A cloning kit (Fermentas, USA). .. All the white clones were subjected for re-amplification of insert by M13 forward and reverse primers (Fermentas, USA) and restricted with Hae III enzyme (Fermentas, USA).

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  • 99
    Millipore multiscreen pcr clean up filter plates
    Multiscreen Pcr Clean Up Filter Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiscreen pcr clean up filter plates/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    multiscreen pcr clean up filter plates - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Millipore genelute pcr clean up kit
    Genelute Pcr Clean Up Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genelute pcr clean up kit/product/Millipore
    Average 99 stars, based on 422 article reviews
    Price from $9.99 to $1999.99
    genelute pcr clean up kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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