genejet gel extraction kit  (Thermo Fisher)


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    Name:
    GeneJET Gel Extraction Kit
    Description:
    Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low melting point agarose gels run in either TAE or TBE buffer The kit utilizes a proprietary silica based membrane technology in the form of a convenient spin column The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95 Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel The entire procedure takes just 15 minutes and the isolated DNA is ready to use in all common downstream applications including ligation restriction digestion PCR sequencing and labeling Highlights• Highly efficient up to 95 recoveries in the range of 100 bp to 10 kb• Fast procedure takes only 15 minutes• Convenient spin columns are capped and assembled with collection tubesApplications• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including • FastDigest or conventional restriction digestion• Automated fluorescent or radioactive sequencing• PCR• In situ hybridization• Labeling• Cloning
    Catalog Number:
    k0691
    Price:
    None
    Applications:
    Agarose Gel Extraction|DNA & RNA Purification & Analysis|DNA Extraction
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher genejet gel extraction kit
    Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low melting point agarose gels run in either TAE or TBE buffer The kit utilizes a proprietary silica based membrane technology in the form of a convenient spin column The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95 Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel The entire procedure takes just 15 minutes and the isolated DNA is ready to use in all common downstream applications including ligation restriction digestion PCR sequencing and labeling Highlights• Highly efficient up to 95 recoveries in the range of 100 bp to 10 kb• Fast procedure takes only 15 minutes• Convenient spin columns are capped and assembled with collection tubesApplications• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including • FastDigest or conventional restriction digestion• Automated fluorescent or radioactive sequencing• PCR• In situ hybridization• Labeling• Cloning
    https://www.bioz.com/result/genejet gel extraction kit/product/Thermo Fisher
    Average 99 stars, based on 761 article reviews
    Price from $9.99 to $1999.99
    genejet gel extraction kit - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses
    Article Snippet: .. The resulting L and M dsRNAs were visualized by gel electrophoresis and gel-purified using GeneJet Gel Extraction Kit (Thermo Fisher Scientific, Vilnius, Lithuania). .. 2.4. cDNA Synthesis, Amplification, and Cloning Viral dsRNA cDNA synthesis and amplification were performed as described [ ], with modifications.

    Amplification:

    Article Title: Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs
    Article Snippet: .. DNA bands of 400–450 bp, corresponding to the 16S rRNA gene amplicon, were excised and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s instructions. .. Purified amplicons were used for library preparation and pyrosequencing.

    Agarose Gel Electrophoresis:

    Article Title: Candidate Genes and Molecular Markers Correlated to Physiological Traits for Heat Tolerance in Fine Fescue Cultivars
    Article Snippet: .. PCR product was first visualized in 1% agarose gel under UV light, recovered using GeneJET gel extraction and DNA cleanup micro kit (Thermo Fisher Scientific), and used for Sanger sequencing (GenScript, Piscataway, NJ, USA). .. SNPs were identified using NovoSNP program [ ], which could detect heterozygous sequence data based on a cumulative scoring scheme.

    Article Title: Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD
    Article Snippet: .. The reaction system is as follows: 98∘ C 1 min; then 30 cycles (98∘ C, 10 s; 50∘ C, 30 s; 72∘ C, and 30 s); 72∘ C, 5 min. After the PCR, the amplicons were electrophoresis in 2% agarose gel and then extracted by using the gel extraction kit (GeneJET, Thermo Fisher Scientific, Waltham, MA, United States). .. The gene library was constructed by using the Ion Plus Fragment Library Kit 48 rxns (Thermo Fisher Scientific, Waltham, MA, United States).

    Article Title: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae
    Article Snippet: .. The ligation products were then treated with Hin dIII, analysed by agarose gel electrophoresis, and the bands corresponding to the fusion products (2,284 and 2,224 bp, respectively) were purified from the gel using GeneJET Gel Extraction kit (Thermo Fisher Scientific Biosciences). .. The purified fusion products were ligated into pBluescript-KS(II) using Hin dIII in such a way that the fusion products were under control of the vector-borne lac promoter (Plac ).

    Ligation:

    Article Title: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae
    Article Snippet: .. The ligation products were then treated with Hin dIII, analysed by agarose gel electrophoresis, and the bands corresponding to the fusion products (2,284 and 2,224 bp, respectively) were purified from the gel using GeneJET Gel Extraction kit (Thermo Fisher Scientific Biosciences). .. The purified fusion products were ligated into pBluescript-KS(II) using Hin dIII in such a way that the fusion products were under control of the vector-borne lac promoter (Plac ).

    Purification:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. The 3Cs synthesis product was then affinity purified and desalted using a Thermo Fisher Scientific GeneJET Gel Extraction Kit (Thermo Fisher, K0692) according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product, mixed and applied to two purification columns, which were centrifuged for 3 min at 460 g (2,500 rpm in a Sigma-Aldrich 1–14 table top centrifuge). .. The flow-through was applied a second time to the same purification column to maximize yield and centrifuged for 3 min at 460 g. DNA was eluted in 40 µl warm water.

    Article Title: Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs
    Article Snippet: .. DNA bands of 400–450 bp, corresponding to the 16S rRNA gene amplicon, were excised and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s instructions. .. Purified amplicons were used for library preparation and pyrosequencing.

    Article Title: The Comparative Genomics of Human Respiratory Syncytial Virus Subgroups A and B: Genetic Variability and Molecular Evolutionary Dynamics
    Article Snippet: .. PCR products were purified from 1% agarose gels by the use of a GeneJET gel extraction kit (Fermentas) and were sequenced according the conventional Sanger technique. .. Fragments ranging between 650 and 1,400 nucleotides in length were sequenced on an ABI 3730 48-capillary DNA analyzer using BigDye Terminator 3.1 (ABI) and sequence-specific primers (see Table S2 in the supplemental material).

    Article Title: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae
    Article Snippet: .. The ligation products were then treated with Hin dIII, analysed by agarose gel electrophoresis, and the bands corresponding to the fusion products (2,284 and 2,224 bp, respectively) were purified from the gel using GeneJET Gel Extraction kit (Thermo Fisher Scientific Biosciences). .. The purified fusion products were ligated into pBluescript-KS(II) using Hin dIII in such a way that the fusion products were under control of the vector-borne lac promoter (Plac ).

    Electrophoresis:

    Article Title: Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD
    Article Snippet: .. The reaction system is as follows: 98∘ C 1 min; then 30 cycles (98∘ C, 10 s; 50∘ C, 30 s; 72∘ C, and 30 s); 72∘ C, 5 min. After the PCR, the amplicons were electrophoresis in 2% agarose gel and then extracted by using the gel extraction kit (GeneJET, Thermo Fisher Scientific, Waltham, MA, United States). .. The gene library was constructed by using the Ion Plus Fragment Library Kit 48 rxns (Thermo Fisher Scientific, Waltham, MA, United States).

    Polymerase Chain Reaction:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Candidate Genes and Molecular Markers Correlated to Physiological Traits for Heat Tolerance in Fine Fescue Cultivars
    Article Snippet: .. PCR product was first visualized in 1% agarose gel under UV light, recovered using GeneJET gel extraction and DNA cleanup micro kit (Thermo Fisher Scientific), and used for Sanger sequencing (GenScript, Piscataway, NJ, USA). .. SNPs were identified using NovoSNP program [ ], which could detect heterozygous sequence data based on a cumulative scoring scheme.

    Article Title: The Comparative Genomics of Human Respiratory Syncytial Virus Subgroups A and B: Genetic Variability and Molecular Evolutionary Dynamics
    Article Snippet: .. PCR products were purified from 1% agarose gels by the use of a GeneJET gel extraction kit (Fermentas) and were sequenced according the conventional Sanger technique. .. Fragments ranging between 650 and 1,400 nucleotides in length were sequenced on an ABI 3730 48-capillary DNA analyzer using BigDye Terminator 3.1 (ABI) and sequence-specific primers (see Table S2 in the supplemental material).

    Article Title: Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD
    Article Snippet: .. The reaction system is as follows: 98∘ C 1 min; then 30 cycles (98∘ C, 10 s; 50∘ C, 30 s; 72∘ C, and 30 s); 72∘ C, 5 min. After the PCR, the amplicons were electrophoresis in 2% agarose gel and then extracted by using the gel extraction kit (GeneJET, Thermo Fisher Scientific, Waltham, MA, United States). .. The gene library was constructed by using the Ion Plus Fragment Library Kit 48 rxns (Thermo Fisher Scientific, Waltham, MA, United States).

    Sequencing:

    Article Title: Candidate Genes and Molecular Markers Correlated to Physiological Traits for Heat Tolerance in Fine Fescue Cultivars
    Article Snippet: .. PCR product was first visualized in 1% agarose gel under UV light, recovered using GeneJET gel extraction and DNA cleanup micro kit (Thermo Fisher Scientific), and used for Sanger sequencing (GenScript, Piscataway, NJ, USA). .. SNPs were identified using NovoSNP program [ ], which could detect heterozygous sequence data based on a cumulative scoring scheme.

    Gel Extraction:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Candidate Genes and Molecular Markers Correlated to Physiological Traits for Heat Tolerance in Fine Fescue Cultivars
    Article Snippet: .. PCR product was first visualized in 1% agarose gel under UV light, recovered using GeneJET gel extraction and DNA cleanup micro kit (Thermo Fisher Scientific), and used for Sanger sequencing (GenScript, Piscataway, NJ, USA). .. SNPs were identified using NovoSNP program [ ], which could detect heterozygous sequence data based on a cumulative scoring scheme.

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. The 3Cs synthesis product was then affinity purified and desalted using a Thermo Fisher Scientific GeneJET Gel Extraction Kit (Thermo Fisher, K0692) according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product, mixed and applied to two purification columns, which were centrifuged for 3 min at 460 g (2,500 rpm in a Sigma-Aldrich 1–14 table top centrifuge). .. The flow-through was applied a second time to the same purification column to maximize yield and centrifuged for 3 min at 460 g. DNA was eluted in 40 µl warm water.

    Article Title: Exploring the Fecal Microbial Composition and Metagenomic Functional Capacities Associated With Feed Efficiency in Commercial DLY Pigs
    Article Snippet: .. DNA bands of 400–450 bp, corresponding to the 16S rRNA gene amplicon, were excised and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s instructions. .. Purified amplicons were used for library preparation and pyrosequencing.

    Article Title: The Comparative Genomics of Human Respiratory Syncytial Virus Subgroups A and B: Genetic Variability and Molecular Evolutionary Dynamics
    Article Snippet: .. PCR products were purified from 1% agarose gels by the use of a GeneJET gel extraction kit (Fermentas) and were sequenced according the conventional Sanger technique. .. Fragments ranging between 650 and 1,400 nucleotides in length were sequenced on an ABI 3730 48-capillary DNA analyzer using BigDye Terminator 3.1 (ABI) and sequence-specific primers (see Table S2 in the supplemental material).

    Article Title: Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD
    Article Snippet: .. The reaction system is as follows: 98∘ C 1 min; then 30 cycles (98∘ C, 10 s; 50∘ C, 30 s; 72∘ C, and 30 s); 72∘ C, 5 min. After the PCR, the amplicons were electrophoresis in 2% agarose gel and then extracted by using the gel extraction kit (GeneJET, Thermo Fisher Scientific, Waltham, MA, United States). .. The gene library was constructed by using the Ion Plus Fragment Library Kit 48 rxns (Thermo Fisher Scientific, Waltham, MA, United States).

    Article Title: Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses
    Article Snippet: .. The resulting L and M dsRNAs were visualized by gel electrophoresis and gel-purified using GeneJet Gel Extraction Kit (Thermo Fisher Scientific, Vilnius, Lithuania). .. 2.4. cDNA Synthesis, Amplification, and Cloning Viral dsRNA cDNA synthesis and amplification were performed as described [ ], with modifications.

    Article Title: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae
    Article Snippet: .. The ligation products were then treated with Hin dIII, analysed by agarose gel electrophoresis, and the bands corresponding to the fusion products (2,284 and 2,224 bp, respectively) were purified from the gel using GeneJET Gel Extraction kit (Thermo Fisher Scientific Biosciences). .. The purified fusion products were ligated into pBluescript-KS(II) using Hin dIII in such a way that the fusion products were under control of the vector-borne lac promoter (Plac ).

    Affinity Purification:

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. The 3Cs synthesis product was then affinity purified and desalted using a Thermo Fisher Scientific GeneJET Gel Extraction Kit (Thermo Fisher, K0692) according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product, mixed and applied to two purification columns, which were centrifuged for 3 min at 460 g (2,500 rpm in a Sigma-Aldrich 1–14 table top centrifuge). .. The flow-through was applied a second time to the same purification column to maximize yield and centrifuged for 3 min at 460 g. DNA was eluted in 40 µl warm water.

    Binding Assay:

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. The 3Cs synthesis product was then affinity purified and desalted using a Thermo Fisher Scientific GeneJET Gel Extraction Kit (Thermo Fisher, K0692) according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product, mixed and applied to two purification columns, which were centrifuged for 3 min at 460 g (2,500 rpm in a Sigma-Aldrich 1–14 table top centrifuge). .. The flow-through was applied a second time to the same purification column to maximize yield and centrifuged for 3 min at 460 g. DNA was eluted in 40 µl warm water.

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  • 99
    Thermo Fisher genejet gel extraction kit
    Genejet Gel Extraction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genejet gel extraction kit/product/Thermo Fisher
    Average 99 stars, based on 761 article reviews
    Price from $9.99 to $1999.99
    genejet gel extraction kit - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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