genejet gel extrac tion kit  (Thermo Fisher)


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    Name:
    GeneJET Gel Extraction Kit
    Description:
    Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low melting point agarose gels run in either TAE or TBE buffer The kit utilizes a proprietary silica based membrane technology in the form of a convenient spin column The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95 Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel The entire procedure takes just 15 minutes and the isolated DNA is ready to use in all common downstream applications including ligation restriction digestion PCR sequencing and labeling Highlights• Highly efficient up to 95 recoveries in the range of 100 bp to 10 kb• Fast procedure takes only 15 minutes• Convenient spin columns are capped and assembled with collection tubesApplications• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including • FastDigest or conventional restriction digestion• Automated fluorescent or radioactive sequencing• PCR• In situ hybridization• Labeling• Cloning
    Catalog Number:
    k0691
    Price:
    None
    Applications:
    Agarose Gel Extraction|DNA & RNA Purification & Analysis|DNA Extraction
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher genejet gel extrac tion kit
    Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low melting point agarose gels run in either TAE or TBE buffer The kit utilizes a proprietary silica based membrane technology in the form of a convenient spin column The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95 Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel The entire procedure takes just 15 minutes and the isolated DNA is ready to use in all common downstream applications including ligation restriction digestion PCR sequencing and labeling Highlights• Highly efficient up to 95 recoveries in the range of 100 bp to 10 kb• Fast procedure takes only 15 minutes• Convenient spin columns are capped and assembled with collection tubesApplications• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including • FastDigest or conventional restriction digestion• Automated fluorescent or radioactive sequencing• PCR• In situ hybridization• Labeling• Cloning
    https://www.bioz.com/result/genejet gel extrac tion kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genejet gel extrac tion kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Agarose Gel Electrophoresis:

    Article Title: Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak. Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak
    Article Snippet: .. The PCR products were verified using agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific). .. The purified products were quantified using a NanoDrop 2000 UV‐Vis spectrophotometer and mixed in equimolar amounts.

    Article Title: CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases
    Article Snippet: .. Linearized plasmids were purified following agarose gel electrophoresis using GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Vilnius, Lithuania). .. The linear purified DNAs were sequenced using MZ-438 and MZ-437 oligonucleotides as primers ( ).

    Article Title: Cell Biology of Homologous Recombination in Yeast
    Article Snippet: .. All PCR fragments are agarose gel purified using GeneJET™ Gel Extraction Kit (Fermentas, Germany). .. The following chemicals were used: bleomycin (Sigma-Aldrich) , zeocin (Invitrogen), camptothecin (Sigma-Aldrich), methyl methanesulfonate (Sigma-Aldrich), raffinose (Sigma-Aldrich), galactose (Sigma-Aldrich), petroleum jelly (Vaseline; VWR Scientific Products), beeswax (Sigma-Aldrich), lanolin (Sigma-Aldrich), NuSieve® GTG® low-melt agarose (Lonza Rockland, Inc., Rockland, ME), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich).

    Purification:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator
    Article Snippet: .. The reverse cross-linked immuoprecipitated DNA fragments were purified using GeneJET gel extraction kit (Thermo Scientific) followed by PCR analyses for the p53-responsive DNA elements on the promoters of human NGFR and p21 and mouse p21 using the following primers, 5-GACTCCAACCTTGCTAATTCCT-3 and 5-TGACCTTCACCAGTTCTCACT-3 for human NGFR , 5-GCTCCCTCATGGGCAAACTCACT-3 and 5-TGGCTGGTCTACCTGGCTCCTCT-3 for human p21 , and 5-CCTTTCTATCAGCCCCAGAGGATACC-3 and 5-GACCCCAAAATGACAAAGTGACAA-3 for mouse p21 . .. Reverse transcription and quantitative PCR analyses Total RNA was isolated from cells using Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol.

    Article Title: Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak. Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak
    Article Snippet: .. The PCR products were verified using agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific). .. The purified products were quantified using a NanoDrop 2000 UV‐Vis spectrophotometer and mixed in equimolar amounts.

    Article Title: Alpha-Ketoglutarate in Low-Protein Diets for Growing Pigs: Effects on Cecal Microbial Communities and Parameters of Microbial Metabolism
    Article Snippet: .. All amplicons were in the size range of 400–450 bp, and were purified using a GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Carlsbad, CA, United States). .. Following quantitation, equal concentrations of the purified amplicons were combined into a single tube.

    Article Title: CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases
    Article Snippet: .. Linearized plasmids were purified following agarose gel electrophoresis using GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Vilnius, Lithuania). .. The linear purified DNAs were sequenced using MZ-438 and MZ-437 oligonucleotides as primers ( ).

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Cell Biology of Homologous Recombination in Yeast
    Article Snippet: .. All PCR fragments are agarose gel purified using GeneJET™ Gel Extraction Kit (Fermentas, Germany). .. The following chemicals were used: bleomycin (Sigma-Aldrich) , zeocin (Invitrogen), camptothecin (Sigma-Aldrich), methyl methanesulfonate (Sigma-Aldrich), raffinose (Sigma-Aldrich), galactose (Sigma-Aldrich), petroleum jelly (Vaseline; VWR Scientific Products), beeswax (Sigma-Aldrich), lanolin (Sigma-Aldrich), NuSieve® GTG® low-melt agarose (Lonza Rockland, Inc., Rockland, ME), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich).

    Polymerase Chain Reaction:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator
    Article Snippet: .. The reverse cross-linked immuoprecipitated DNA fragments were purified using GeneJET gel extraction kit (Thermo Scientific) followed by PCR analyses for the p53-responsive DNA elements on the promoters of human NGFR and p21 and mouse p21 using the following primers, 5-GACTCCAACCTTGCTAATTCCT-3 and 5-TGACCTTCACCAGTTCTCACT-3 for human NGFR , 5-GCTCCCTCATGGGCAAACTCACT-3 and 5-TGGCTGGTCTACCTGGCTCCTCT-3 for human p21 , and 5-CCTTTCTATCAGCCCCAGAGGATACC-3 and 5-GACCCCAAAATGACAAAGTGACAA-3 for mouse p21 . .. Reverse transcription and quantitative PCR analyses Total RNA was isolated from cells using Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol.

    Article Title: Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak. Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak
    Article Snippet: .. The PCR products were verified using agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific). .. The purified products were quantified using a NanoDrop 2000 UV‐Vis spectrophotometer and mixed in equimolar amounts.

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Cell Biology of Homologous Recombination in Yeast
    Article Snippet: .. All PCR fragments are agarose gel purified using GeneJET™ Gel Extraction Kit (Fermentas, Germany). .. The following chemicals were used: bleomycin (Sigma-Aldrich) , zeocin (Invitrogen), camptothecin (Sigma-Aldrich), methyl methanesulfonate (Sigma-Aldrich), raffinose (Sigma-Aldrich), galactose (Sigma-Aldrich), petroleum jelly (Vaseline; VWR Scientific Products), beeswax (Sigma-Aldrich), lanolin (Sigma-Aldrich), NuSieve® GTG® low-melt agarose (Lonza Rockland, Inc., Rockland, ME), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich).

    Gel Extraction:

    Article Title: CRISPR-mediated isolation of specific megabase segments of genomic DNA
    Article Snippet: .. Based on the weight, each gel slice is dissolved at 55°C in the appropriate volume of Binding Buffer as described and provided by the GeneJET Gel Extraction Kit (Thermo Scientific). .. To shear the DNA segments into 200–500 bp fragments, each liquified gel slice is subjected to 12 cycles of sonication on the Fisher Scientific™ Model 505 Sonic Dismembrator equipped with a 0.32 cm probe at 4°C set to 25% amplitude with 20 s of sonication followed by 40 s rest.

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Finally, the cDNA was purified using a PCR-purification kit (GeneJET Gel Extraction Kit, Thermo Scientific, Milian, Vernier, Switzerland), and eluted in 50 μl Elution Buffer. .. Second-strand PCR To generate double stranded DNA, with the appropriate adaptors for Illumina sequencing, as well as addition of a four nucleotide EMOTE barcode which serves to identify the RNA sample and pool, a PCR reaction was prepared for each RT-reaction: 10 μl PCR-purified RT-reaction, 27 μl water, 10 μl Q5 Polymerase buffer, 1.5 μl dNTP (2.5 mM each), 1.5 μl 100nM of one of primers D6A, D6B, D6C, etc. (each with a unique EMOTE barcode, Table ), 1.5 μl 10 uM Primer B-PE-PCR20, and 0.5 μl Q5® Hot Start High-Fidelity DNA Polymerase (NEB).

    Article Title: Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator
    Article Snippet: .. The reverse cross-linked immuoprecipitated DNA fragments were purified using GeneJET gel extraction kit (Thermo Scientific) followed by PCR analyses for the p53-responsive DNA elements on the promoters of human NGFR and p21 and mouse p21 using the following primers, 5-GACTCCAACCTTGCTAATTCCT-3 and 5-TGACCTTCACCAGTTCTCACT-3 for human NGFR , 5-GCTCCCTCATGGGCAAACTCACT-3 and 5-TGGCTGGTCTACCTGGCTCCTCT-3 for human p21 , and 5-CCTTTCTATCAGCCCCAGAGGATACC-3 and 5-GACCCCAAAATGACAAAGTGACAA-3 for mouse p21 . .. Reverse transcription and quantitative PCR analyses Total RNA was isolated from cells using Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol.

    Article Title: Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak. Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak
    Article Snippet: .. The PCR products were verified using agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific). .. The purified products were quantified using a NanoDrop 2000 UV‐Vis spectrophotometer and mixed in equimolar amounts.

    Article Title: Alpha-Ketoglutarate in Low-Protein Diets for Growing Pigs: Effects on Cecal Microbial Communities and Parameters of Microbial Metabolism
    Article Snippet: .. All amplicons were in the size range of 400–450 bp, and were purified using a GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Carlsbad, CA, United States). .. Following quantitation, equal concentrations of the purified amplicons were combined into a single tube.

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Cell Biology of Homologous Recombination in Yeast
    Article Snippet: .. All PCR fragments are agarose gel purified using GeneJET™ Gel Extraction Kit (Fermentas, Germany). .. The following chemicals were used: bleomycin (Sigma-Aldrich) , zeocin (Invitrogen), camptothecin (Sigma-Aldrich), methyl methanesulfonate (Sigma-Aldrich), raffinose (Sigma-Aldrich), galactose (Sigma-Aldrich), petroleum jelly (Vaseline; VWR Scientific Products), beeswax (Sigma-Aldrich), lanolin (Sigma-Aldrich), NuSieve® GTG® low-melt agarose (Lonza Rockland, Inc., Rockland, ME), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich).

    Binding Assay:

    Article Title: CRISPR-mediated isolation of specific megabase segments of genomic DNA
    Article Snippet: .. Based on the weight, each gel slice is dissolved at 55°C in the appropriate volume of Binding Buffer as described and provided by the GeneJET Gel Extraction Kit (Thermo Scientific). .. To shear the DNA segments into 200–500 bp fragments, each liquified gel slice is subjected to 12 cycles of sonication on the Fisher Scientific™ Model 505 Sonic Dismembrator equipped with a 0.32 cm probe at 4°C set to 25% amplitude with 20 s of sonication followed by 40 s rest.

    Plasmid Preparation:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

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  • 99
    Thermo Fisher genejet gel extrac tion kit
    Genejet Gel Extrac Tion Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genejet gel extrac tion kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genejet gel extrac tion kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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