genechip wt terminal labeling kit  (Thermo Fisher)


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    Name:
    GeneChip WT Terminal Labeling Kit
    Description:
    GeneChip WT Sense Target Labeling and Control Reagents 900652 and GeneChip WT cDNA Synthesis and Amplification Kits 900672 and 900673 are being discontinued in June 2010 The Whole Transcript WT Sense Target Labeling Assay has an updated protocol The GeneChip WT Whole Transcript Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip WT Sense Target ST Labeling Assay The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase UDG and apurinic apyrimidinic endonuclease 1 APE 1 The typical input material is random primed single stranded DNA Following fragmentation the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase TdT in the presence of a proprietary biotinylated compound GeneChip DNA Labeling Reagent Both components are also provided in the WT Terminal Labeling Kit The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip Human Exon 1 0 ST Array
    Catalog Number:
    900670
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    DNA & RNA Microarray Analysis|Microarray Analysis
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    Structured Review

    Thermo Fisher genechip wt terminal labeling kit
    Results of C/EBPβ and C/EBPδ ChIP-SEQ and microarray. a) MEF WT cells were incubated in the presence or absence of a combination of the cyclic AMP-elevating agents, forskolin and rolipram (F/R). Following stimulation cells were fixed, chromatin was isolated and then immunoprecipitated (ChIP'd) with anti-C/EBPβ or C/EBPδ antibodies, as described in Materials and methods. ChIP'd DNA samples were then sequenced (ChIP-SEQ) on an Illumina GA IIx DNA sequencer. ChIP analysis of the resulting DNA sequences was then performed using the Homer (version 3.9) suite of tools ( biowhat.ucsd.edu/homer/ngs/index.html ). The figure shows part of the Homer analysis indicating that aligned sequences from each ChIP experiments contained bone fide C/EBP consensus binding motifs, hence validating the experimental technique. b) MEF WT, MEF C/EBPβ −/− and MEF C/EBPδ −/− cells were stimulated for 5 h in the presence or absence of F/R. Cells were then harvested, RNA isolated, biotinylated sense-strand DNA synthesised and hybridised to mouse whole genome <t>GeneChip</t> ® ST Arrays (Affymatrix). A Venn diagram was then generated to illustrate the degree of overlap between genes identified by ChIP-SEQ as containing consensus, F/R-dependent C/EBP-binding sites and F/R-induced changes in MEF gene expression.
    GeneChip WT Sense Target Labeling and Control Reagents 900652 and GeneChip WT cDNA Synthesis and Amplification Kits 900672 and 900673 are being discontinued in June 2010 The Whole Transcript WT Sense Target Labeling Assay has an updated protocol The GeneChip WT Whole Transcript Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip WT Sense Target ST Labeling Assay The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase UDG and apurinic apyrimidinic endonuclease 1 APE 1 The typical input material is random primed single stranded DNA Following fragmentation the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase TdT in the presence of a proprietary biotinylated compound GeneChip DNA Labeling Reagent Both components are also provided in the WT Terminal Labeling Kit The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip Human Exon 1 0 ST Array
    https://www.bioz.com/result/genechip wt terminal labeling kit/product/Thermo Fisher
    Average 99 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    genechip wt terminal labeling kit - by Bioz Stars, 2021-01
    99/100 stars

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    Images

    1) Product Images from "Genomic analysis of the role of transcription factor C/EBP? in the regulation of cell behaviour on nanometric grooves"

    Article Title: Genomic analysis of the role of transcription factor C/EBP? in the regulation of cell behaviour on nanometric grooves

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2012.11.036

    Results of C/EBPβ and C/EBPδ ChIP-SEQ and microarray. a) MEF WT cells were incubated in the presence or absence of a combination of the cyclic AMP-elevating agents, forskolin and rolipram (F/R). Following stimulation cells were fixed, chromatin was isolated and then immunoprecipitated (ChIP'd) with anti-C/EBPβ or C/EBPδ antibodies, as described in Materials and methods. ChIP'd DNA samples were then sequenced (ChIP-SEQ) on an Illumina GA IIx DNA sequencer. ChIP analysis of the resulting DNA sequences was then performed using the Homer (version 3.9) suite of tools ( biowhat.ucsd.edu/homer/ngs/index.html ). The figure shows part of the Homer analysis indicating that aligned sequences from each ChIP experiments contained bone fide C/EBP consensus binding motifs, hence validating the experimental technique. b) MEF WT, MEF C/EBPβ −/− and MEF C/EBPδ −/− cells were stimulated for 5 h in the presence or absence of F/R. Cells were then harvested, RNA isolated, biotinylated sense-strand DNA synthesised and hybridised to mouse whole genome GeneChip ® ST Arrays (Affymatrix). A Venn diagram was then generated to illustrate the degree of overlap between genes identified by ChIP-SEQ as containing consensus, F/R-dependent C/EBP-binding sites and F/R-induced changes in MEF gene expression.
    Figure Legend Snippet: Results of C/EBPβ and C/EBPδ ChIP-SEQ and microarray. a) MEF WT cells were incubated in the presence or absence of a combination of the cyclic AMP-elevating agents, forskolin and rolipram (F/R). Following stimulation cells were fixed, chromatin was isolated and then immunoprecipitated (ChIP'd) with anti-C/EBPβ or C/EBPδ antibodies, as described in Materials and methods. ChIP'd DNA samples were then sequenced (ChIP-SEQ) on an Illumina GA IIx DNA sequencer. ChIP analysis of the resulting DNA sequences was then performed using the Homer (version 3.9) suite of tools ( biowhat.ucsd.edu/homer/ngs/index.html ). The figure shows part of the Homer analysis indicating that aligned sequences from each ChIP experiments contained bone fide C/EBP consensus binding motifs, hence validating the experimental technique. b) MEF WT, MEF C/EBPβ −/− and MEF C/EBPδ −/− cells were stimulated for 5 h in the presence or absence of F/R. Cells were then harvested, RNA isolated, biotinylated sense-strand DNA synthesised and hybridised to mouse whole genome GeneChip ® ST Arrays (Affymatrix). A Venn diagram was then generated to illustrate the degree of overlap between genes identified by ChIP-SEQ as containing consensus, F/R-dependent C/EBP-binding sites and F/R-induced changes in MEF gene expression.

    Techniques Used: Chromatin Immunoprecipitation, Microarray, Incubation, Isolation, Immunoprecipitation, Next-Generation Sequencing, Binding Assay, Generated, Expressing

    2) Product Images from "5Z-7-Oxozeanol Inhibits the Effects of TGFβ1 on Human Gingival Fibroblasts"

    Article Title: 5Z-7-Oxozeanol Inhibits the Effects of TGFβ1 on Human Gingival Fibroblasts

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0123689

    (5Z)-7-oxozeaenol inhibits TGFβ1-induced mRNA expression in human gingival fibroblasts. (A) Human gingival fibroblasts were serum starved overnight and pre-treated with (5Z)-7-oxozeaenol (400 nM) or DMSO for 45 min followed by treatment with TGFβ1 (4ngml -1 (90 pM)) ligand or left untreated. Total RNA was harvested six hours later and subjected to gene expression profiling using GeneChip Human Gene 1.0 ST arrays (N = 2) as described in Methods. 147 genes were up-regulated in response to TGFβ1 (1.7 fold induction compared to DMSO control group) and 139 genes of the latter group were found to be (5 Z )-7-Oxozeaenol sensitive. (B) Human gingival fibroblasts were treated as in (A) and subject to TaqMan RT-qPCR analysis using the indicated probe/primer set. 18S RNA was used as the internal control. (N = 3; averages+/-SEM are shown. * = p
    Figure Legend Snippet: (5Z)-7-oxozeaenol inhibits TGFβ1-induced mRNA expression in human gingival fibroblasts. (A) Human gingival fibroblasts were serum starved overnight and pre-treated with (5Z)-7-oxozeaenol (400 nM) or DMSO for 45 min followed by treatment with TGFβ1 (4ngml -1 (90 pM)) ligand or left untreated. Total RNA was harvested six hours later and subjected to gene expression profiling using GeneChip Human Gene 1.0 ST arrays (N = 2) as described in Methods. 147 genes were up-regulated in response to TGFβ1 (1.7 fold induction compared to DMSO control group) and 139 genes of the latter group were found to be (5 Z )-7-Oxozeaenol sensitive. (B) Human gingival fibroblasts were treated as in (A) and subject to TaqMan RT-qPCR analysis using the indicated probe/primer set. 18S RNA was used as the internal control. (N = 3; averages+/-SEM are shown. * = p

    Techniques Used: Expressing, Quantitative RT-PCR

    3) Product Images from "The Impact of Gut Microbiota on Gender-Specific Differences in Immunity"

    Article Title: The Impact of Gut Microbiota on Gender-Specific Differences in Immunity

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.00754

    Microbiota-dependent differences in gender-specific immunity. Whole-genome gene expression in the ileum of male and female germ-free (GF) mice ( n = 5) that received the gut microbiota from male or female conventional mice was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Genes that were significantly differentially expressed ( p
    Figure Legend Snippet: Microbiota-dependent differences in gender-specific immunity. Whole-genome gene expression in the ileum of male and female germ-free (GF) mice ( n = 5) that received the gut microbiota from male or female conventional mice was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Genes that were significantly differentially expressed ( p

    Techniques Used: Expressing, Mouse Assay

    Microbiota-independent differences in immunity between males and females. Whole-genome gene expression in the ileum of male and female conventional (conv) mice ( n = 5 per group) or male and female germ-free (GF) mice ( n = 4) was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Genes that were significantly differentially expressed ( p
    Figure Legend Snippet: Microbiota-independent differences in immunity between males and females. Whole-genome gene expression in the ileum of male and female conventional (conv) mice ( n = 5 per group) or male and female germ-free (GF) mice ( n = 4) was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Genes that were significantly differentially expressed ( p

    Techniques Used: Expressing, Mouse Assay

    Male microbiota influences DNA replication and the cell cycle. Whole-genome gene expression in the ileum of male and female germ-free (GF) mice ( n = 4), or male and female GF recipient mice ( n = 5) that received the gut microbiota from male or female conventional mice was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Only genes that were significantly differentially expressed ( p
    Figure Legend Snippet: Male microbiota influences DNA replication and the cell cycle. Whole-genome gene expression in the ileum of male and female germ-free (GF) mice ( n = 4), or male and female GF recipient mice ( n = 5) that received the gut microbiota from male or female conventional mice was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. Only genes that were significantly differentially expressed ( p

    Techniques Used: Expressing, Mouse Assay

    4) Product Images from "β2→1-Fructans Modulate the Immune System In Vivo in a Microbiota-Dependent and -Independent Fashion"

    Article Title: β2→1-Fructans Modulate the Immune System In Vivo in a Microbiota-Dependent and -Independent Fashion

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.00154

    β2→1-fructans induce differential gene expression in the ileum . Whole-genome gene expression in the ileum of germ-free mice ( n = 4 per group) treated with short-chain β2→1-fructans (short), long-chain β2→1-fructans (long), or water was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. (A) Number of genes that were considerably differentially expressed ( p
    Figure Legend Snippet: β2→1-fructans induce differential gene expression in the ileum . Whole-genome gene expression in the ileum of germ-free mice ( n = 4 per group) treated with short-chain β2→1-fructans (short), long-chain β2→1-fructans (long), or water was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. (A) Number of genes that were considerably differentially expressed ( p

    Techniques Used: Expressing, Mouse Assay

    Differential gene expression in the ileum after β2→1-fructan treatment . Whole-genome gene expression in the ileum of C57BL/6 mice ( n = 4 per group) treated with short-chain β2→1-fructans (short), long-chain β2→1-fructans (long), or water was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. (A) Number of genes that were considerably differentially expressed ( p
    Figure Legend Snippet: Differential gene expression in the ileum after β2→1-fructan treatment . Whole-genome gene expression in the ileum of C57BL/6 mice ( n = 4 per group) treated with short-chain β2→1-fructans (short), long-chain β2→1-fructans (long), or water was assessed with Affymetrix GeneChip Mouse Gene 1.1 ST arrays. (A) Number of genes that were considerably differentially expressed ( p

    Techniques Used: Expressing, Mouse Assay

    5) Product Images from "Long-Term Transcriptomic Effects of Prebiotics and Synbiotics Delivered In Ovo in Broiler Chickens"

    Article Title: Long-Term Transcriptomic Effects of Prebiotics and Synbiotics Delivered In Ovo in Broiler Chickens

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0168899

    Differentially expressed genes (DEG) in cecal tonsils, spleen, and large intestine of the animals that were treated in ovo with prebiotics and synbiotics. Gene expression was determined by whole-genome microarray GeneChip 1.1 ST (Affymetrix, Santa Clara, CA, US) for four substances injected in ovo . Experimental groups were injected, in ovo , with prebiotics: inulin (P1) and GOS (P2), and synbiotics: inulin with L . lactis subsp. lactis IBB2955 (S1) and GOS with L . lactis subsp. cremoris IBB 477 (S2). The cut-off value was set at 1.66
    Figure Legend Snippet: Differentially expressed genes (DEG) in cecal tonsils, spleen, and large intestine of the animals that were treated in ovo with prebiotics and synbiotics. Gene expression was determined by whole-genome microarray GeneChip 1.1 ST (Affymetrix, Santa Clara, CA, US) for four substances injected in ovo . Experimental groups were injected, in ovo , with prebiotics: inulin (P1) and GOS (P2), and synbiotics: inulin with L . lactis subsp. lactis IBB2955 (S1) and GOS with L . lactis subsp. cremoris IBB 477 (S2). The cut-off value was set at 1.66

    Techniques Used: In Ovo, Expressing, Microarray, Injection

    6) Product Images from "AGRONOMICS1: A New Resource for Arabidopsis Transcriptome Profiling"

    Article Title: AGRONOMICS1: A New Resource for Arabidopsis Transcriptome Profiling

    Journal: Plant Physiology

    doi: 10.1104/pp.109.150185

    Comparison of RNA amplification and labeling methods. A, Two batches of RNA (flower and leaf) were used for amplification and labeling as technical replicates with the WT Ovation Pico System, the GeneChip One-Cycle cDNA Synthesis kit, and the GeneChip
    Figure Legend Snippet: Comparison of RNA amplification and labeling methods. A, Two batches of RNA (flower and leaf) were used for amplification and labeling as technical replicates with the WT Ovation Pico System, the GeneChip One-Cycle cDNA Synthesis kit, and the GeneChip

    Techniques Used: Amplification, Labeling

    7) Product Images from "Effect of Chronic Pioglitazone Treatment on Hepatic Gene Expression Profile in Obese C57BL/6J Mice"

    Article Title: Effect of Chronic Pioglitazone Treatment on Hepatic Gene Expression Profile in Obese C57BL/6J Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms160612213

    Validation of GeneChip data with RT-PCR. The expression of eight genes was analyzed using GeneChip and RT-PCR. The changes in expression of these genes were similar in the direction and magnitude between the two techniques.
    Figure Legend Snippet: Validation of GeneChip data with RT-PCR. The expression of eight genes was analyzed using GeneChip and RT-PCR. The changes in expression of these genes were similar in the direction and magnitude between the two techniques.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing

    8) Product Images from "MicroRNA-124 Targets Tip110 Expression and Regulates Hematopoiesis"

    Article Title: MicroRNA-124 Targets Tip110 Expression and Regulates Hematopoiesis

    Journal: Stem Cells and Development

    doi: 10.1089/scd.2015.0030

    Regulatory pathways of hematopoiesis associated with Tip110 expression. Mouse embryonic fibroblasts (MEFs) isolated from Tip110 transgenic mice and congenic wild-type mice were used to isolate total RNA for the Affymetrix GeneChip ® Mouse Exon
    Figure Legend Snippet: Regulatory pathways of hematopoiesis associated with Tip110 expression. Mouse embryonic fibroblasts (MEFs) isolated from Tip110 transgenic mice and congenic wild-type mice were used to isolate total RNA for the Affymetrix GeneChip ® Mouse Exon

    Techniques Used: Expressing, Isolation, Transgenic Assay, Mouse Assay

    Related Articles

    Microarray:

    Article Title: Chicken interferome: avian interferon-stimulated genes identified by microarray and RNA-seq of primary chick embryo fibroblasts treated with a chicken type I interferon (IFN-α)
    Article Snippet: .. RNA samples were processed for microarray with the GeneChip® Chicken Genome Array (Affymetrix) using the GeneChip® 3′ IVT Express Kit (Affymetrix) or for microarray with the Chicken Gene 1.0 ST Array (Affymetrix) using the Ambion (Paisley, UK) WT Expression Kit for Affymetrix GeneChip® Whole Transcript (WT) Expression Arrays (Ambion) and the GeneChip WT Terminal Labelling and Controls Kit (Ambion), following the manufacturers’ instructions, as described previously [ ]. .. Total RNA (100 ng) was used as input and quality checks were performed using the 2100 Bioanalyzer at all stages suggested by the manufacturer.

    Amplification:

    Article Title: Characterization of a P-Rex1 gene signature in breast cancer cells
    Article Snippet: .. Total RNA for each sample was obtained using the miRNeasy kit (Qiagen; Valencia, CA), reversed transcribed to cDNA (GeneChip® Whole Transcript cDNA Synthesis and Amplification Kit, Affymetrix; Santa Clara, CA), fragmented, and end labeled (GeneChip® WT Terminal Labelling Kit, Affymetrix) for further hybridization to an Affymetrix GeneChip Human Gene 1.0 ST Array (GPL6244) according to the standard protocols at the University of Pennsylvania Molecular Profiling Facility. .. RNA quality was analyzed using the Agilent Bioanalyzer Nano assay.

    Labeling:

    Article Title: High-resolution characterization of CPD hotspot formation in human fibroblasts
    Article Snippet: .. Samples were fragmented to ∼60 bp by treatment with uracil DNA glycosylase and labeled with biotin using the GeneChip WT Double-Stranded DNA Terminal Labeling Kit (Affymetrix) before probing Affymetrix GeneChip Human Tiling 2.0 R Array A microarrays. ..

    Article Title: 5Z-7-Oxozeanol Inhibits the Effects of TGFβ1 on Human Gingival Fibroblasts
    Article Snippet: .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). .. Total RNA was first converted to cDNA, followed by in vitro transcription to make cRNA.

    Article Title: Characterization of a P-Rex1 gene signature in breast cancer cells
    Article Snippet: .. Total RNA for each sample was obtained using the miRNeasy kit (Qiagen; Valencia, CA), reversed transcribed to cDNA (GeneChip® Whole Transcript cDNA Synthesis and Amplification Kit, Affymetrix; Santa Clara, CA), fragmented, and end labeled (GeneChip® WT Terminal Labelling Kit, Affymetrix) for further hybridization to an Affymetrix GeneChip Human Gene 1.0 ST Array (GPL6244) according to the standard protocols at the University of Pennsylvania Molecular Profiling Facility. .. RNA quality was analyzed using the Agilent Bioanalyzer Nano assay.

    Article Title: The Impact of Gut Microbiota on Gender-Specific Differences in Immunity
    Article Snippet: .. Total RNA (100 ng) was labeled utilizing the Ambion WT Expression kit (Life Technologies Ltd., Bleiswijk, the Netherlands) and the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA, USA). .. After labeling, samples were hybridized to Affymetrix GeneChip Mouse Gene 1.1 ST arrays.

    Article Title: β2→1-Fructans Modulate the Immune System In Vivo in a Microbiota-Dependent and -Independent Fashion
    Article Snippet: .. Total RNA (100 ng) was labeled utilizing the Ambion WT Expression kit (Life Technologies Ltd., Bleiswijk, the Netherlands) and the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA, USA). .. After labeling, samples were hybridized to Affymetrix GeneChip Mouse Gene 1.1 ST arrays.

    Expressing:

    Article Title: 5Z-7-Oxozeanol Inhibits the Effects of TGFβ1 on Human Gingival Fibroblasts
    Article Snippet: .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). .. Total RNA was first converted to cDNA, followed by in vitro transcription to make cRNA.

    Article Title: The Impact of Gut Microbiota on Gender-Specific Differences in Immunity
    Article Snippet: .. Total RNA (100 ng) was labeled utilizing the Ambion WT Expression kit (Life Technologies Ltd., Bleiswijk, the Netherlands) and the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA, USA). .. After labeling, samples were hybridized to Affymetrix GeneChip Mouse Gene 1.1 ST arrays.

    Article Title: β2→1-Fructans Modulate the Immune System In Vivo in a Microbiota-Dependent and -Independent Fashion
    Article Snippet: .. Total RNA (100 ng) was labeled utilizing the Ambion WT Expression kit (Life Technologies Ltd., Bleiswijk, the Netherlands) and the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA, USA). .. After labeling, samples were hybridized to Affymetrix GeneChip Mouse Gene 1.1 ST arrays.

    Article Title: Chicken interferome: avian interferon-stimulated genes identified by microarray and RNA-seq of primary chick embryo fibroblasts treated with a chicken type I interferon (IFN-α)
    Article Snippet: .. RNA samples were processed for microarray with the GeneChip® Chicken Genome Array (Affymetrix) using the GeneChip® 3′ IVT Express Kit (Affymetrix) or for microarray with the Chicken Gene 1.0 ST Array (Affymetrix) using the Ambion (Paisley, UK) WT Expression Kit for Affymetrix GeneChip® Whole Transcript (WT) Expression Arrays (Ambion) and the GeneChip WT Terminal Labelling and Controls Kit (Ambion), following the manufacturers’ instructions, as described previously [ ]. .. Total RNA (100 ng) was used as input and quality checks were performed using the 2100 Bioanalyzer at all stages suggested by the manufacturer.

    Hybridization:

    Article Title: A Brassica Exon Array for Whole-Transcript Gene Expression Profiling
    Article Snippet: .. The end labelling, hybridisation, washing and scanning were performed according to the GeneChip® WT terminal labelling and hybridisation user manual ( www.affymetrix.com ), and scanned using an Affymetrix 3000 7G scanner. .. Following scanning, non-scaled RNA signal intensity files (.cel) were generated using the Command Console software (Affymetrix).

    Article Title: 5Z-7-Oxozeanol Inhibits the Effects of TGFβ1 on Human Gingival Fibroblasts
    Article Snippet: .. Single stranded complimentary DNA (sscDNA) was prepared from 200 ng of total RNA as per the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays ( http://www.ambion.com/techlib/prot/fm_4411973.pdf , Applied Biosystems, Carlsbad, CA) and the Affymetrix GeneChip WT Terminal Labeling kit and Hybridization User Manual ( http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf , Affymetrix, Santa Clara, CA). .. Total RNA was first converted to cDNA, followed by in vitro transcription to make cRNA.

    Article Title: Characterization of a P-Rex1 gene signature in breast cancer cells
    Article Snippet: .. Total RNA for each sample was obtained using the miRNeasy kit (Qiagen; Valencia, CA), reversed transcribed to cDNA (GeneChip® Whole Transcript cDNA Synthesis and Amplification Kit, Affymetrix; Santa Clara, CA), fragmented, and end labeled (GeneChip® WT Terminal Labelling Kit, Affymetrix) for further hybridization to an Affymetrix GeneChip Human Gene 1.0 ST Array (GPL6244) according to the standard protocols at the University of Pennsylvania Molecular Profiling Facility. .. RNA quality was analyzed using the Agilent Bioanalyzer Nano assay.

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    • genechip wt terminal labeling kit  (Thermo Fisher)
    99
    Thermo Fisher genechip wt terminal labeling kit
    Genechip Wt Terminal Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip wt terminal labeling kit/product/Thermo Fisher
    Average 99 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    genechip wt terminal labeling kit - by Bioz Stars, 2021-01
    99/100 stars
    		  Buy from Supplier

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