genechip hybridization oven 640  (Thermo Fisher)


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    Structured Review

    Thermo Fisher genechip hybridization oven 640
    Genechip Hybridization Oven 640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip hybridization oven 640/product/Thermo Fisher
    Average 99 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    genechip hybridization oven 640 - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: Microarray-based transcriptome analyses Aliquots of three cultures (3.0 × 108 cells/mL) of the WT and ΔhmgA strains grown in LM at 30°C for 48 h with constant shaking at 200 rpm were harvested for total RNA extraction. .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix).

    Amplification:

    Article Title: Dysregulation of AKT3 along with a small panel of mRNAs stratifies high-grade serous ovarian cancer from both normal epithelia and benign tumor tissues
    Article Snippet: Exon Microarray and Sample Hybridization 3 μg of SPIA amplified cDNA was used to do the sense–strand cDNA (ST-cDNA) conversion using the WT- Ovation Exon Module (NuGEN Technologies Inc., San Carlos, CA). .. Microarray hybridization and scanning was performed using GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450, and GeneChip Scanner 3000 7G with Autoloader respectively (Affymetrix, Inc., Santa Clara, CA) [ ].

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: The amplified product of the Ovation Pico WTA system is optimized for gene expression analysis applications on microarrays. .. Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA).

    Fluorescence:

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix). .. Raw fluorescence intensity values were normalized by quantile normalization and differential gene expression was calculated by ANOVA using the software package SAS JMP7 Genomics (SAS Institute, Cary, NC).

    Isolation:

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: RNA was isolated using the RiboPure™-Bacteria Kit (Life Technologies, Grand Island, NY, USA), treated with DNase and 10 μg of total RNA from each culture was further purified using the MICROBExpress ™ Bacterial mRNA Enrichment Kit (Life Technologies) according to the manufacturer's specifications. .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix).

    Generated:

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA). .. The microarray data were analyzed with the Affymetrix GeneChip Command Console and processed with the use of Command Console (Affymetrix, Inc. Santa Clara, CA) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings (Affymetrix, Inc. Santa Clara, CA).

    Labeling:

    Article Title: Dysregulation of AKT3 along with a small panel of mRNAs stratifies high-grade serous ovarian cancer from both normal epithelia and benign tumor tissues
    Article Snippet: 5 μg ST-cDNA was fragmented and labeled with FL-Ovation cDNA Biotin Module V2 kit (NuGEN Technologies Inc., San Carlos, CA) and hybridized using Affymetrix Human Exon 1.0 ST arrays (Affymetrix, Inc., Santa Clara, CA). .. Microarray hybridization and scanning was performed using GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450, and GeneChip Scanner 3000 7G with Autoloader respectively (Affymetrix, Inc., Santa Clara, CA) [ ].

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: The normalized RNA was labeled, purified, fragmented and hybridized to a custom Affymetrix microarray (520694F) according to standard protocols (Affymetrix, Santa Clara, CA, USA). .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix).

    Article Title: Genome-Wide Transcription Profiling of the Early Phase of Biofilm Formation by Candida albicans †
    Article Snippet: .. RNA labeling, target hybridization, washing, staining, and scanning were performed by using a GeneChip Hybridization Oven 640, a GeneChip Fluidics Station 400, and a GeneArray scanner (Affymetrix) as described previously ( ). .. GeneChips were scanned, and the resulting image files were used to calculate, normalize, and compare the hybridization intensity data by using the Microarray Suite 5.0 software (Affymetrix).

    Purification:

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: .. Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix). ..

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: The normalized RNA was labeled, purified, fragmented and hybridized to a custom Affymetrix microarray (520694F) according to standard protocols (Affymetrix, Santa Clara, CA, USA). .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix).

    Microarray:

    Article Title: Dysregulation of AKT3 along with a small panel of mRNAs stratifies high-grade serous ovarian cancer from both normal epithelia and benign tumor tissues
    Article Snippet: .. Microarray hybridization and scanning was performed using GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450, and GeneChip Scanner 3000 7G with Autoloader respectively (Affymetrix, Inc., Santa Clara, CA) [ ]. ..

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA). .. The microarray data were analyzed with the Affymetrix GeneChip Command Console and processed with the use of Command Console (Affymetrix, Inc. Santa Clara, CA) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings (Affymetrix, Inc. Santa Clara, CA).

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: Paragraph title: Microarray-based transcriptome analyses ... All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix).

    Article Title: Genome-Wide Transcription Profiling of the Early Phase of Biofilm Formation by Candida albicans †
    Article Snippet: Paragraph title: DNA microarray design and analysis. ... RNA labeling, target hybridization, washing, staining, and scanning were performed by using a GeneChip Hybridization Oven 640, a GeneChip Fluidics Station 400, and a GeneArray scanner (Affymetrix) as described previously ( ).

    Incubation:

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix). .. Hybridization signal intensities were analyzed with the GeneChip® Operating Software (GCOS) to generate raw image files (.DAT) and simplified image files (.CEL) with intensities assigned to each of the corresponding probe positions.

    Expressing:

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: The amplified product of the Ovation Pico WTA system is optimized for gene expression analysis applications on microarrays. .. Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA).

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: Paragraph title: Gene expression microarrays and data analysis ... Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix).

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix). .. The data collected was used to profile the expression levels of 4831 open reading frames.

    Staining:

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA). .. The microarrays were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneChip 7G 3000 Scanner.

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: .. Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix). ..

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix). .. Hybridization signal intensities were analyzed with the GeneChip® Operating Software (GCOS) to generate raw image files (.DAT) and simplified image files (.CEL) with intensities assigned to each of the corresponding probe positions.

    Article Title: Genome-Wide Transcription Profiling of the Early Phase of Biofilm Formation by Candida albicans †
    Article Snippet: .. RNA labeling, target hybridization, washing, staining, and scanning were performed by using a GeneChip Hybridization Oven 640, a GeneChip Fluidics Station 400, and a GeneArray scanner (Affymetrix) as described previously ( ). .. GeneChips were scanned, and the resulting image files were used to calculate, normalize, and compare the hybridization intensity data by using the Microarray Suite 5.0 software (Affymetrix).

    Hybridization:

    Article Title: Dysregulation of AKT3 along with a small panel of mRNAs stratifies high-grade serous ovarian cancer from both normal epithelia and benign tumor tissues
    Article Snippet: .. Microarray hybridization and scanning was performed using GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450, and GeneChip Scanner 3000 7G with Autoloader respectively (Affymetrix, Inc., Santa Clara, CA) [ ]. ..

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: .. Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA). .. The microarrays were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneChip 7G 3000 Scanner.

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: .. Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix). ..

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: .. All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix). .. Hybridization signal intensities were analyzed with the GeneChip® Operating Software (GCOS) to generate raw image files (.DAT) and simplified image files (.CEL) with intensities assigned to each of the corresponding probe positions.

    Article Title: Genome-Wide Transcription Profiling of the Early Phase of Biofilm Formation by Candida albicans †
    Article Snippet: .. RNA labeling, target hybridization, washing, staining, and scanning were performed by using a GeneChip Hybridization Oven 640, a GeneChip Fluidics Station 400, and a GeneArray scanner (Affymetrix) as described previously ( ). .. GeneChips were scanned, and the resulting image files were used to calculate, normalize, and compare the hybridization intensity data by using the Microarray Suite 5.0 software (Affymetrix).

    Software:

    Article Title: RNA Microarray Analysis of Macroscopically Normal Articular Cartilage from Knees Undergoing Partial Medial Meniscectomy: Potential Prediction of the Risk for Developing Osteoarthritis
    Article Snippet: Following fragmentation with NuGEN Encore Biotin Module, cDNA was hybridized into the GeneChip Hybridization Oven 640 for 18 hours at 45°C on Affymetrix Gene 2.0 ST Array (Affymetrix Inc. Santa Clara, CA). .. The microarray data were analyzed with the Affymetrix GeneChip Command Console and processed with the use of Command Console (Affymetrix, Inc. Santa Clara, CA) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings (Affymetrix, Inc. Santa Clara, CA).

    Article Title: Profiling of gallbladder carcinoma reveals distinct miRNA profiles and activation of STAT1 by the tumor suppressive miRNA-145-5p
    Article Snippet: Complementary RNA was purified, fragmented and hybridized using a GeneChip Hybridization oven 640, stained using a GeneChip Fluidics Station 450 and scanned with a GeneChip Scanner 3000 (Affymetrix). .. Raw fluorescence intensity values were normalized by quantile normalization and differential gene expression was calculated by ANOVA using the software package SAS JMP7 Genomics (SAS Institute, Cary, NC).

    Article Title: Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness
    Article Snippet: All hybridizations incubated for 16 h at 49°C in the GeneChip® Hybridization Oven 640 at 60 rpm and the microarrays were then washed and stained with the GeneChip® Fluidics Station 450 and scanned using the GeneChip® Scanner 7G (Affymetrix). .. Hybridization signal intensities were analyzed with the GeneChip® Operating Software (GCOS) to generate raw image files (.DAT) and simplified image files (.CEL) with intensities assigned to each of the corresponding probe positions.