geneamp pcr system 2700 thermocycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher geneamp pcr system 2700 thermocycler
    Geneamp Pcr System 2700 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneamp pcr system 2700 thermocycler/product/Thermo Fisher
    Average 96 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    geneamp pcr system 2700 thermocycler - by Bioz Stars, 2020-02
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. The pdh1 gene coding for pyranose dehydrogenase from the basidiomycete A. meleagris (GenBank accession number AY753307) has been previously isolated and cloned into the pCR Blunt II TOPO vector (Kittl et al. ).

    Amplification:

    Article Title: Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease
    Article Snippet: ABV RNAs were reverse-transcribed and amplified with a continuous RT-PCR method by using a One Step RT-PCR kit (QIAGEN) according to the manufacturer’s instructions. .. Amplifications were performed in a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA).

    Article Title: Genetic chimerism of Vitis vinifera cv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis
    Article Snippet: Paragraph title: Amplification of microsatellites and polymorphism detection ... The PCR was carried out using a GeneAmp® PCR System 2700 thermocycler (Applied Biosystems).

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems). .. 2.7 Massive DNA semiconductor sequencing Amplicons were high-throughput sequenced as previously described ( ).

    Article Title: 16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “ Clostridium neonatale”
    Article Snippet: PCR was carried out using randomly amplified polymorphic DNA (RAPD) analysis beads (GE Healthcare, Vélizy-Villacoublay, France) as previously described ( ). .. PCR (1 cycle at 95°C for 5 min followed by 45 cycles at 95°C for 1 min, 36°C for 1 min, and 72°C for 2 min) was performed on a GeneAmp PCR System 2700 thermocycler (Life Technologies, Saint Aubin, France).

    Article Title: Hybridization-Induced Aggregation Technology for Practical Clinical Testing
    Article Snippet: A GeneAmp PCR System 2700 thermocycler (Applied Biosystems) was used with the following thermocycling conditions: 3 minutes at 95°C; followed by 50 cycles of 15 seconds at 95°C, 15 seconds at 58°C, and 5 seconds at 72°C; then finally 2 minutes at 72°C. .. The PCR products were analyzed using Agilent 2100 DNA 1000 Series II kits and instrumentation (Agilent Technologies) to confirm amplification.

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA). .. PCR products were electrophoresed on 1.5% agarose gels to check the size of the amplified fragments by comparison to molecular weight marker GeneRuler 1kb DNA ladder (Thermo Scientific, Waltham, MA, USA).

    Article Title: Male Genital Morphology and Its Influence on Female Mating Preferences and Paternity Success in Guppies
    Article Snippet: PCR amplifications were performed following the methods outlined in Gasparini et al. on a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems, CA, USA). .. Amplified fragments were separated by electrophoresis on an ABI 3100 sequencer (ABI PRISM, Applied Biosystems), using 400 HD ROX (Perkin-Elmer, Applied Biosystems) as a size standard ( http://www.bmr-genomics.com ).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification. .. Quantitative RT-PCR reactions were performed using SYBRGreen Master Mix according to the manufacturer's instructions (Qiagen).

    Article Title: Reconciling Deep Calibration and Demographic History: Bayesian Inference of Post Glacial Colonization Patterns in Carcinus aestuarii (Nardo, 1847) and C. maenas (Linnaeus, 1758)
    Article Snippet: We amplified a 569 base-pair (bp) fragment of the mitochondrial COI (cytochrome oxidase subunit I) gene using primers developed by Roman and Palumbi for C. maenas . .. PCR products were obtained in a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: The DNA sample (100 ng) was amplified by PCR using a commercially available kit (HotStar Taq DNA polymerase, Qiagen). .. All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA).

    Quantitative RT-PCR:

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR (qRT-PCR) ... One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Real-time Polymerase Chain Reaction:

    Article Title: The Murine Caecal MicroRNA Signature Depends on the Presence of the Endogenous Microbiota
    Article Snippet: .. Real-Time PCR validation of individual microRNAs expression Ten ng of total RNA (n=5 per group) was reverse transcribed with the Taqman® MicroRNA Reverse Transcription Kit and primers specific for miR-455 (Assay ID: 002455) and the endogenous control snoRNA135 genes (Assay ID: 001230) (Applied Biosystems) in Applied Biosystems' GeneAmp® PCR System 2700 Thermocycler according to the manufacturer's protocol. .. Real time PCR was then conducted using undiluted cDNA, TaqMan MicroRNA Assays (miR-455 assay ID: 002455 and snoRNA135 assay ID: 001230) and the TaqMan 2X Universal PCR Master Mix, No AmpEraseUNGa (Applied Biosystems) in a 10 µl PCR reaction.

    TLDA Assay:

    Article Title: The Murine Caecal MicroRNA Signature Depends on the Presence of the Endogenous Microbiota
    Article Snippet: Real-Time PCR validation of individual microRNAs expression Ten ng of total RNA (n=5 per group) was reverse transcribed with the Taqman® MicroRNA Reverse Transcription Kit and primers specific for miR-455 (Assay ID: 002455) and the endogenous control snoRNA135 genes (Assay ID: 001230) (Applied Biosystems) in Applied Biosystems' GeneAmp® PCR System 2700 Thermocycler according to the manufacturer's protocol. .. Results were expressed as fold change between germ-free and conventional mice as calculated by ΔΔCt method after normalization to sno-135 gene, which was shown to be equally expressed in the caeca of the two groups of mice by the TLDA experiments.

    Expressing:

    Article Title: The Murine Caecal MicroRNA Signature Depends on the Presence of the Endogenous Microbiota
    Article Snippet: .. Real-Time PCR validation of individual microRNAs expression Ten ng of total RNA (n=5 per group) was reverse transcribed with the Taqman® MicroRNA Reverse Transcription Kit and primers specific for miR-455 (Assay ID: 002455) and the endogenous control snoRNA135 genes (Assay ID: 001230) (Applied Biosystems) in Applied Biosystems' GeneAmp® PCR System 2700 Thermocycler according to the manufacturer's protocol. .. Real time PCR was then conducted using undiluted cDNA, TaqMan MicroRNA Assays (miR-455 assay ID: 002455 and snoRNA135 assay ID: 001230) and the TaqMan 2X Universal PCR Master Mix, No AmpEraseUNGa (Applied Biosystems) in a 10 µl PCR reaction.

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. Escherichia coli strain DH5α-T1 was used for subcloning and BL21 DE3 for expression (Invitrogen).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: Quantitative real-time RT-PCR (qRT-PCR) Total RNA was isolated from ileal samples using TRIZOL reagent (Invitrogen, Carlsbad, CA) for qRT-PCR to validate microbial colonization-induced changes in the expression of genes involved in selected pathways. .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Electroporation:

    Article Title: Directed evolution of the substrate specificity of dialkylglycine decarboxylase ☆
    Article Snippet: All PCR was carried out using or an Applied Biosystems GeneAmp PCR System 2700 thermocycler. .. Bacterial transformations were carried out using a Bio-Rad Micropulser electroporation apparatus.

    Ligation:

    Article Title: Viral genome integration of canine papillomavirus 16
    Article Snippet: The genome walker adaptors were then ligated to the digested libraries in a reaction mixture containing 4.8 µl digested purified DNA, 1.9 µl genome walker adaptor, 0.8 µl 10X ligation buffer, and 0.5 µl T4 DNA ligase. .. PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease
    Article Snippet: Paragraph title: Nucleic Acid Extraction and RT-PCR ... Amplifications were performed in a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification. .. Quantitative RT-PCR reactions were performed using SYBRGreen Master Mix according to the manufacturer's instructions (Qiagen).

    Imaging:

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: The quantity and quality of purified DNA were assessed by 260/280 absorbance using a NanoDrop Lite Spectrophotometer (Thermo Scientific); electrophoretic fractionation in 0.5% agarose gels, stained with Midori Green (Cat. MG02, Nippon Genetics); and recorded using BIO-RAD Molecular Imager Gel DOC XR + Imaging System. .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Sequencing:

    Article Title: Cross-Reaction of Stenotrophomonas and Xanthomonas Species in a 23S rRNA Gene-Directed PCR for Detection of S. maltophilia ▿
    Article Snippet: Amplifications were performed on a GeneAmp PCR system 2700 thermocycler (Applied Biosystems) as follows: 95°C for 10 min and then 45 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 60 s, with final extension at 72°C for 7 min. A product of approximately 531 bp was expected. .. Sequencing of products was performed (ABI PRISM 3100 Avant genetic analyzer; Applied Biosystems) and the S. acidaminiphila ATCC 700916T , Stenotrophomonas nitritireducens ATCC BAA-12T , Stenotrophomonas rhizophila ATCC BAA-473T , Xanthomonas axonopodis pv. citri DAR 65864, and Xanthomonas campestris pv. campestris DAR 65808 sequences have the GenBank accession no. .

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: Paragraph title: PCR and sequence analysis ... The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA).

    Cellular Antioxidant Activity Assay:

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: The primers were felGAPDH-For (5’-GAT TGT CAG CAA TGC CTC CT-3’) and felGAPDH-Rev (5’-AAG CAG GGA TGA TGT TCT GG-3’). .. All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA).

    Molecular Weight:

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA). .. PCR products were electrophoresed on 1.5% agarose gels to check the size of the amplified fragments by comparison to molecular weight marker GeneRuler 1kb DNA ladder (Thermo Scientific, Waltham, MA, USA).

    DNA Extraction:

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: 2.6 16S rDNA libraries preparation for high throughput sequencing Bacterial Genomic DNA was extracted from 250 μL slurry from C-MFCs using Power soil DNA Isolation Kit (MoBio Laboratories). .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Isolation:

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. The pdh1 gene coding for pyranose dehydrogenase from the basidiomycete A. meleagris (GenBank accession number AY753307) has been previously isolated and cloned into the pCR Blunt II TOPO vector (Kittl et al. ).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: Quantitative real-time RT-PCR (qRT-PCR) Total RNA was isolated from ileal samples using TRIZOL reagent (Invitrogen, Carlsbad, CA) for qRT-PCR to validate microbial colonization-induced changes in the expression of genes involved in selected pathways. .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Subcloning:

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. Escherichia coli strain DH5α-T1 was used for subcloning and BL21 DE3 for expression (Invitrogen).

    Purification:

    Article Title: Viral genome integration of canine papillomavirus 16
    Article Snippet: The genome walker adaptors were then ligated to the digested libraries in a reaction mixture containing 4.8 µl digested purified DNA, 1.9 µl genome walker adaptor, 0.8 µl 10X ligation buffer, and 0.5 µl T4 DNA ligase. .. PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler.

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: The quantity and quality of purified DNA were assessed by 260/280 absorbance using a NanoDrop Lite Spectrophotometer (Thermo Scientific); electrophoretic fractionation in 0.5% agarose gels, stained with Midori Green (Cat. MG02, Nippon Genetics); and recorded using BIO-RAD Molecular Imager Gel DOC XR + Imaging System. .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Article Title: 16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “ Clostridium neonatale”
    Article Snippet: Briefly, 10 ng of purified DNA and 25 pm of primer 1 (5′-GGTGCGGGAA-3′) or primer 6 (5′-CCCGTCAGCA-3′) was used in a total volume of 25 μl. .. PCR (1 cycle at 95°C for 5 min followed by 45 cycles at 95°C for 1 min, 36°C for 1 min, and 72°C for 2 min) was performed on a GeneAmp PCR System 2700 thermocycler (Life Technologies, Saint Aubin, France).

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA). .. Amplified DNA fragments were purified with a PureLink Quick PCR Purification Kit (Thermo Scientific, Waltham, MA, USA) and sequenced using the reverse primer on each locus.

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: RNA was purified with the RNeasy Mini kit and residual DNA removed using the RNase-Free DNase set (Qiagen). .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Polymerase Chain Reaction:

    Article Title: Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease
    Article Snippet: .. Amplifications were performed in a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Viral genome integration of canine papillomavirus 16
    Article Snippet: .. PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler. .. Secondary PCR was performed using 1 µl of the PCR reaction diluted in 49 µl water with the adaptor primer 2 and a gene specific primer in the combinations listed in .

    Article Title: Genetic chimerism of Vitis vinifera cv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis
    Article Snippet: .. The PCR was carried out using a GeneAmp® PCR System 2700 thermocycler (Applied Biosystems). ..

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems). .. 2.7 Massive DNA semiconductor sequencing Amplicons were high-throughput sequenced as previously described ( ).

    Article Title: Cross-Reaction of Stenotrophomonas and Xanthomonas Species in a 23S rRNA Gene-Directed PCR for Detection of S. maltophilia ▿
    Article Snippet: .. Amplifications were performed on a GeneAmp PCR system 2700 thermocycler (Applied Biosystems) as follows: 95°C for 10 min and then 45 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 60 s, with final extension at 72°C for 7 min. A product of approximately 531 bp was expected. ..

    Article Title: 16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “ Clostridium neonatale”
    Article Snippet: .. PCR (1 cycle at 95°C for 5 min followed by 45 cycles at 95°C for 1 min, 36°C for 1 min, and 72°C for 2 min) was performed on a GeneAmp PCR System 2700 thermocycler (Life Technologies, Saint Aubin, France). .. DNA purification from 24-h TGYH broth bacterial culture was performed with InstaGene matrix (Bio-Rad).

    Article Title: The Murine Caecal MicroRNA Signature Depends on the Presence of the Endogenous Microbiota
    Article Snippet: .. Real-Time PCR validation of individual microRNAs expression Ten ng of total RNA (n=5 per group) was reverse transcribed with the Taqman® MicroRNA Reverse Transcription Kit and primers specific for miR-455 (Assay ID: 002455) and the endogenous control snoRNA135 genes (Assay ID: 001230) (Applied Biosystems) in Applied Biosystems' GeneAmp® PCR System 2700 Thermocycler according to the manufacturer's protocol. .. Real time PCR was then conducted using undiluted cDNA, TaqMan MicroRNA Assays (miR-455 assay ID: 002455 and snoRNA135 assay ID: 001230) and the TaqMan 2X Universal PCR Master Mix, No AmpEraseUNGa (Applied Biosystems) in a 10 µl PCR reaction.

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: .. Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. The pdh1 gene coding for pyranose dehydrogenase from the basidiomycete A. meleagris (GenBank accession number AY753307) has been previously isolated and cloned into the pCR Blunt II TOPO vector (Kittl et al. ).

    Article Title: Hybridization-Induced Aggregation Technology for Practical Clinical Testing
    Article Snippet: .. A GeneAmp PCR System 2700 thermocycler (Applied Biosystems) was used with the following thermocycling conditions: 3 minutes at 95°C; followed by 50 cycles of 15 seconds at 95°C, 15 seconds at 58°C, and 5 seconds at 72°C; then finally 2 minutes at 72°C. .. The PCR products were analyzed using Agilent 2100 DNA 1000 Series II kits and instrumentation (Agilent Technologies) to confirm amplification.

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: .. The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA). .. PCR products were electrophoresed on 1.5% agarose gels to check the size of the amplified fragments by comparison to molecular weight marker GeneRuler 1kb DNA ladder (Thermo Scientific, Waltham, MA, USA).

    Article Title: Male Genital Morphology and Its Influence on Female Mating Preferences and Paternity Success in Guppies
    Article Snippet: .. PCR amplifications were performed following the methods outlined in Gasparini et al. on a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems, CA, USA). .. Amplified fragments were separated by electrophoresis on an ABI 3100 sequencer (ABI PRISM, Applied Biosystems), using 400 HD ROX (Perkin-Elmer, Applied Biosystems) as a size standard ( http://www.bmr-genomics.com ).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification. .. Quantitative RT-PCR reactions were performed using SYBRGreen Master Mix according to the manufacturer's instructions (Qiagen).

    Article Title: Directed evolution of the substrate specificity of dialkylglycine decarboxylase ☆
    Article Snippet: .. All PCR was carried out using or an Applied Biosystems GeneAmp PCR System 2700 thermocycler. .. Bacterial transformations were carried out using a Bio-Rad Micropulser electroporation apparatus.

    Article Title: Reconciling Deep Calibration and Demographic History: Bayesian Inference of Post Glacial Colonization Patterns in Carcinus aestuarii (Nardo, 1847) and C. maenas (Linnaeus, 1758)
    Article Snippet: .. PCR products were obtained in a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems). .. PCR reactions and conditions followed Roman and Palumbi (2004) and PCR products were sequenced using an ABI 3130 AVANT automatic capillary sequencer (Applied Biosystems) on both strands.

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: .. All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA). .. An initial activation step of 95° C for 10 minutes was followed by 40 cycles of 1) 30s denaturation at 95° C 2) 1 minute annealing at 58° C, and 3) 1 minute elongation at 72° C. There was a hold for 7 minutes at 72° C and a final hold at 4° C. Omission of the template DNA served as the negative control.

    Salting Out:

    Article Title: Male Genital Morphology and Its Influence on Female Mating Preferences and Paternity Success in Guppies
    Article Snippet: Paternity analysis Genomic DNA was extracted from newborn offspring using the Chelex protocol and from adults using a standard salting out protocol . .. PCR amplifications were performed following the methods outlined in Gasparini et al. on a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems, CA, USA).

    Article Title: Reconciling Deep Calibration and Demographic History: Bayesian Inference of Post Glacial Colonization Patterns in Carcinus aestuarii (Nardo, 1847) and C. maenas (Linnaeus, 1758)
    Article Snippet: Total genomic DNA was extracted from chelipeds using a salting out protocol . .. PCR products were obtained in a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Concentration Assay:

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: Samples of genomic DNA were diluted with distilled water to yield a concentration of 10 ng/µl. .. All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA).

    Mouse Assay:

    Article Title: The Murine Caecal MicroRNA Signature Depends on the Presence of the Endogenous Microbiota
    Article Snippet: Real-Time PCR validation of individual microRNAs expression Ten ng of total RNA (n=5 per group) was reverse transcribed with the Taqman® MicroRNA Reverse Transcription Kit and primers specific for miR-455 (Assay ID: 002455) and the endogenous control snoRNA135 genes (Assay ID: 001230) (Applied Biosystems) in Applied Biosystems' GeneAmp® PCR System 2700 Thermocycler according to the manufacturer's protocol. .. Results were expressed as fold change between germ-free and conventional mice as calculated by ΔΔCt method after normalization to sno-135 gene, which was shown to be equally expressed in the caeca of the two groups of mice by the TLDA experiments.

    Plasmid Preparation:

    Article Title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
    Article Snippet: Nucleic acid amplifications were done employing Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), dNTP mix, oligonucleotide primers (Invitrogen, Carlsbad, CA, USA), and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. The pdh1 gene coding for pyranose dehydrogenase from the basidiomycete A. meleagris (GenBank accession number AY753307) has been previously isolated and cloned into the pCR Blunt II TOPO vector (Kittl et al. ).

    Software:

    Article Title: Genetic chimerism of Vitis vinifera cv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis
    Article Snippet: The PCR was carried out using a GeneAmp® PCR System 2700 thermocycler (Applied Biosystems). .. The PCR fragments were detected with the GeneScan™ analysis software version 3.1 and the alleles were scored using the Genotyper™ DNA fragment analysis software version 2.5.2 (Applied Biosystems).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: Porcine-gene-specific primers were designed by Primer Express Software v2.0 (Applied Biosystems, Foster City, CA) and PRIMER3 algorithm in Biology WorkBench [ ] [see Additional file ]. .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Electrophoresis:

    Article Title: Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease
    Article Snippet: Amplifications were performed in a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. PCR products were subjected to electrophoresis in 1.5% Tris acetate–EDTA agarose gels and stained with ethidium bromide.

    Article Title: Genetic chimerism of Vitis vinifera cv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis
    Article Snippet: The PCR was carried out using a GeneAmp® PCR System 2700 thermocycler (Applied Biosystems). .. The amplification products were separated by capillary electrophoresis and detected with an ABI PRISM 310 Genetic Analyser (Applied Biosystem), using HD400-ROX as an internal size standard.

    Article Title: Male Genital Morphology and Its Influence on Female Mating Preferences and Paternity Success in Guppies
    Article Snippet: PCR amplifications were performed following the methods outlined in Gasparini et al. on a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems, CA, USA). .. Amplified fragments were separated by electrophoresis on an ABI 3100 sequencer (ABI PRISM, Applied Biosystems), using 400 HD ROX (Perkin-Elmer, Applied Biosystems) as a size standard ( http://www.bmr-genomics.com ).

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA). .. All PCR products were separated by electrophoresis through 1% agarose at 80 volts for one hour and then stained with the DNA stain GelRed (Fisher Scientific, Pittsburg, PA).

    Negative Control:

    Article Title: Viral genome integration of canine papillomavirus 16
    Article Snippet: Omission of the DNA template served as a negative control. .. PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler.

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA). .. An initial activation step of 95° C for 10 minutes was followed by 40 cycles of 1) 30s denaturation at 95° C 2) 1 minute annealing at 58° C, and 3) 1 minute elongation at 72° C. There was a hold for 7 minutes at 72° C and a final hold at 4° C. Omission of the template DNA served as the negative control.

    Next-Generation Sequencing:

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: Paragraph title: 16S rDNA libraries preparation for high throughput sequencing ... An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Spectrophotometry:

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: The quantity and quality of purified DNA were assessed by 260/280 absorbance using a NanoDrop Lite Spectrophotometer (Thermo Scientific); electrophoretic fractionation in 0.5% agarose gels, stained with Midori Green (Cat. MG02, Nippon Genetics); and recorded using BIO-RAD Molecular Imager Gel DOC XR + Imaging System. .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Article Title: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets
    Article Snippet: RNA quality was determined to check the integrity of 28S and 18S rRNA using Agilent 2100 Bioanalyzer (Agilent) and total RNA yield was quantified by spectrophotometry. .. One step RT-PCR was performed using Qiagen reagent (Qiagen) according to the manufacturer's instructions and a GeneAmp PCR System 2700 thermocycler (Applied Biosystems) to confirm single product amplification.

    Activation Assay:

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA). .. An initial activation step of 95° C for 10 minutes was followed by 40 cycles of 1) 30s denaturation at 95° C 2) 1 minute annealing at 58° C, and 3) 1 minute elongation at 72° C. There was a hold for 7 minutes at 72° C and a final hold at 4° C. Omission of the template DNA served as the negative control.

    Fractionation:

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: The quantity and quality of purified DNA were assessed by 260/280 absorbance using a NanoDrop Lite Spectrophotometer (Thermo Scientific); electrophoretic fractionation in 0.5% agarose gels, stained with Midori Green (Cat. MG02, Nippon Genetics); and recorded using BIO-RAD Molecular Imager Gel DOC XR + Imaging System. .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    DNA Purification:

    Article Title: 16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “ Clostridium neonatale”
    Article Snippet: DNA purification from 24-h TGYH broth bacterial culture was performed with InstaGene matrix (Bio-Rad, Marnes la Coquette, France) according to the manufacturer's instructions. .. PCR (1 cycle at 95°C for 5 min followed by 45 cycles at 95°C for 1 min, 36°C for 1 min, and 72°C for 2 min) was performed on a GeneAmp PCR System 2700 thermocycler (Life Technologies, Saint Aubin, France).

    Marker:

    Article Title: Comparative Genomics of Field Isolates of Mycobacterium bovis and M. caprae Provides Evidence for Possible Correlates with Bacterial Viability and Virulence
    Article Snippet: The PCR was conducted using 2 μl of DNA with 20 pmol of each primer in a 50 μl reaction PCR Master Mix (Promega, Madison, WI, USA) using a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Carlsbad, CA, USA). .. PCR products were electrophoresed on 1.5% agarose gels to check the size of the amplified fragments by comparison to molecular weight marker GeneRuler 1kb DNA ladder (Thermo Scientific, Waltham, MA, USA).

    Staining:

    Article Title: Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease
    Article Snippet: Amplifications were performed in a GeneAmp PCR System 2700 thermocycler (Applied Biosystems, Foster City, CA, USA). .. PCR products were subjected to electrophoresis in 1.5% Tris acetate–EDTA agarose gels and stained with ethidium bromide.

    Article Title: Electricity generation from Nopal biogas effluent using a surface modified clay cup (cantarito) microbial fuel cell
    Article Snippet: The quantity and quality of purified DNA were assessed by 260/280 absorbance using a NanoDrop Lite Spectrophotometer (Thermo Scientific); electrophoretic fractionation in 0.5% agarose gels, stained with Midori Green (Cat. MG02, Nippon Genetics); and recorded using BIO-RAD Molecular Imager Gel DOC XR + Imaging System. .. An amplification was carried out using GeneAmp PCR System 2700 Thermocycler (Applied Biosystems).

    Article Title: Localization of Felis catus papillomavirus type 2 E6 and E7 RNA in feline cutaneous squamous cell carcinoma
    Article Snippet: All PCR reactions were performed on an Applied Biosystems GeneAmp PCR system 2700 thermocycler (Foster City, CA, USA). .. All PCR products were separated by electrophoresis through 1% agarose at 80 volts for one hour and then stained with the DNA stain GelRed (Fisher Scientific, Pittsburg, PA).

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    Thermo Fisher thermocycler geneamp pcr system 2700
    Thermocycler Geneamp Pcr System 2700, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler geneamp pcr system 2700/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
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    thermocycler geneamp pcr system 2700 - by Bioz Stars, 2020-02
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