geneamp pcr system 2700 thermocycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher geneamp pcr system 2700 thermocycler
    Geneamp Pcr System 2700 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneamp pcr system 2700 thermocycler/product/Thermo Fisher
    Average 96 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    geneamp pcr system 2700 thermocycler - by Bioz Stars, 2020-09
    96/100 stars

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    Related Articles

    Bla VIM Assay:

    Article Title: Gram-Negative Bacteria That Produce Carbapenemases Causing Death Attributed to Recent Foreign Hospitalization
    Article Snippet: .. PCR amplification for bla CTX-M , bla KPC , bla VIM , bla IMP , bla NDM , bla OXA-48-like , bla OXA-23-like , bla OXA-24-like , bla OXA-51-like , and bla OXA-58-like was carried out on the isolates with a GeneAmp thermal cycler, model 9700 (Applied Biosystems, Norwalk, CT), using PCR conditions and primers described previously ( , – ). bla CTX-M , bla NDM , bla OXA-48-like , and bla OXA-23-like were sequenced using PCR conditions and primers described previously ( , , ). .. The qnrA , qnrS , and qnrB genes were amplified by multiplex PCR ( ). aac(6′)-Ib and qepA were amplified in a separate PCR using primers and conditions described previously ( , ).

    Isolation:

    Article Title: WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris
    Article Snippet: .. Gene isolation and transgene construction MtWOX9-1 and MtWOX9-2 genes were isolated from the M. truncatula genome by BLAST search using the AtWOX9 sequence. .. Full-length MtWOX9-1&2 coding sequences were amplified by RT–PCR using total RNA extracted from leaf samples.

    Article Title: Comparison of measurement methods for the characterization of the black carbon emissions from a T63 turboshaft engine burning conventional and Fischer-Tropsch fuels
    Article Snippet: .. A list of the commercially available light absorption and thermal isolation instruments as obtained from the various manufacturers is shown in , which includes their measurement principle, parameters measured, operating range, and lower detection limit. ..

    Article Title: Broad-Spectrum Effects of 4-Aminopyridine to Modulate Amyloid β1–42-Induced Cell Signaling and Functional Responses in Human Microglia
    Article Snippet: .. For effects of Aβ1–42 on Kv expression, microglia were treated with Aβ1–42 for differing time periods: 10 min, 30 min, 1 h, and 2 h subsequent to incubation in serum-free medium for 48 h. Total RNA was isolated using TRIzol (Invitrogen-BRL, Gaithersburg, MD), subjected to DNase treatment, and processed for the first-strand cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (RT) (Invitrogen-BRL). cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA) with Taq polymerase. ..

    Polymerase Chain Reaction:

    Article Title: Pharmacological antagonism of interleukin-8 receptor CXCR2 inhibits inflammatory reactivity and is neuroprotective in an animal model of Alzheimer’s disease
    Article Snippet: .. Total RNA was extracted using Trizol reagent (Invitrogen) and processed using reverse transcriptase; cDNA products were amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA, USA) with Taq polymerase. .. PCR primers (β-actin was used as a reaction control) were as follows: CXCR2: forward, 5′-GTC AGG ATC CAA GTT TAC CTC AAA AAT GG-3′; reverse, 5′-CTT AGG TCG ACG GTC TTA GAG AGT AGT GG-3′.

    Article Title: Gram-Negative Bacteria That Produce Carbapenemases Causing Death Attributed to Recent Foreign Hospitalization
    Article Snippet: .. PCR amplification for bla CTX-M , bla KPC , bla VIM , bla IMP , bla NDM , bla OXA-48-like , bla OXA-23-like , bla OXA-24-like , bla OXA-51-like , and bla OXA-58-like was carried out on the isolates with a GeneAmp thermal cycler, model 9700 (Applied Biosystems, Norwalk, CT), using PCR conditions and primers described previously ( , – ). bla CTX-M , bla NDM , bla OXA-48-like , and bla OXA-23-like were sequenced using PCR conditions and primers described previously ( , , ). .. The qnrA , qnrS , and qnrB genes were amplified by multiplex PCR ( ). aac(6′)-Ib and qepA were amplified in a separate PCR using primers and conditions described previously ( , ).

    Article Title: Broad-Spectrum Effects of 4-Aminopyridine to Modulate Amyloid β1–42-Induced Cell Signaling and Functional Responses in Human Microglia
    Article Snippet: .. For effects of Aβ1–42 on Kv expression, microglia were treated with Aβ1–42 for differing time periods: 10 min, 30 min, 1 h, and 2 h subsequent to incubation in serum-free medium for 48 h. Total RNA was isolated using TRIzol (Invitrogen-BRL, Gaithersburg, MD), subjected to DNase treatment, and processed for the first-strand cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (RT) (Invitrogen-BRL). cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA) with Taq polymerase. ..

    Article Title: ATP stimulates chemokine production via a store-operated calcium entry pathway in C6 glioma cells
    Article Snippet: .. The cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA). ..

    Incubation:

    Article Title: Broad-Spectrum Effects of 4-Aminopyridine to Modulate Amyloid β1–42-Induced Cell Signaling and Functional Responses in Human Microglia
    Article Snippet: .. For effects of Aβ1–42 on Kv expression, microglia were treated with Aβ1–42 for differing time periods: 10 min, 30 min, 1 h, and 2 h subsequent to incubation in serum-free medium for 48 h. Total RNA was isolated using TRIzol (Invitrogen-BRL, Gaithersburg, MD), subjected to DNase treatment, and processed for the first-strand cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (RT) (Invitrogen-BRL). cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA) with Taq polymerase. ..

    Amplification:

    Article Title: Pharmacological antagonism of interleukin-8 receptor CXCR2 inhibits inflammatory reactivity and is neuroprotective in an animal model of Alzheimer’s disease
    Article Snippet: .. Total RNA was extracted using Trizol reagent (Invitrogen) and processed using reverse transcriptase; cDNA products were amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA, USA) with Taq polymerase. .. PCR primers (β-actin was used as a reaction control) were as follows: CXCR2: forward, 5′-GTC AGG ATC CAA GTT TAC CTC AAA AAT GG-3′; reverse, 5′-CTT AGG TCG ACG GTC TTA GAG AGT AGT GG-3′.

    Article Title: Isolation of Campylobacter fetus subsp. fetus from a Patient with Cellulitis
    Article Snippet: .. Amplification was performed in a GeneAmp thermal cycler (Applied Biosystems), with an initial denaturation step (95°C, 4 min) followed by 30 cycles of denaturation at 94°C for 30 s, primer annealing at 50°C for 20 s, and primer extension at 72°C for 1 min. ..

    Article Title: Gram-Negative Bacteria That Produce Carbapenemases Causing Death Attributed to Recent Foreign Hospitalization
    Article Snippet: .. PCR amplification for bla CTX-M , bla KPC , bla VIM , bla IMP , bla NDM , bla OXA-48-like , bla OXA-23-like , bla OXA-24-like , bla OXA-51-like , and bla OXA-58-like was carried out on the isolates with a GeneAmp thermal cycler, model 9700 (Applied Biosystems, Norwalk, CT), using PCR conditions and primers described previously ( , – ). bla CTX-M , bla NDM , bla OXA-48-like , and bla OXA-23-like were sequenced using PCR conditions and primers described previously ( , , ). .. The qnrA , qnrS , and qnrB genes were amplified by multiplex PCR ( ). aac(6′)-Ib and qepA were amplified in a separate PCR using primers and conditions described previously ( , ).

    Article Title: Broad-Spectrum Effects of 4-Aminopyridine to Modulate Amyloid β1–42-Induced Cell Signaling and Functional Responses in Human Microglia
    Article Snippet: .. For effects of Aβ1–42 on Kv expression, microglia were treated with Aβ1–42 for differing time periods: 10 min, 30 min, 1 h, and 2 h subsequent to incubation in serum-free medium for 48 h. Total RNA was isolated using TRIzol (Invitrogen-BRL, Gaithersburg, MD), subjected to DNase treatment, and processed for the first-strand cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (RT) (Invitrogen-BRL). cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA) with Taq polymerase. ..

    Article Title: ATP stimulates chemokine production via a store-operated calcium entry pathway in C6 glioma cells
    Article Snippet: .. The cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA). ..

    Expressing:

    Article Title: Broad-Spectrum Effects of 4-Aminopyridine to Modulate Amyloid β1–42-Induced Cell Signaling and Functional Responses in Human Microglia
    Article Snippet: .. For effects of Aβ1–42 on Kv expression, microglia were treated with Aβ1–42 for differing time periods: 10 min, 30 min, 1 h, and 2 h subsequent to incubation in serum-free medium for 48 h. Total RNA was isolated using TRIzol (Invitrogen-BRL, Gaithersburg, MD), subjected to DNase treatment, and processed for the first-strand cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (RT) (Invitrogen-BRL). cDNA products were then amplified by PCR using a GeneAmp thermal cycler (Applied Biosystems, Foster City, CA) with Taq polymerase. ..

    Sequencing:

    Article Title: WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris
    Article Snippet: .. Gene isolation and transgene construction MtWOX9-1 and MtWOX9-2 genes were isolated from the M. truncatula genome by BLAST search using the AtWOX9 sequence. .. Full-length MtWOX9-1&2 coding sequences were amplified by RT–PCR using total RNA extracted from leaf samples.

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  • 88
    Thermo Fisher thermocycler geneamp pcr system 2700
    Thermocycler Geneamp Pcr System 2700, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler geneamp pcr system 2700/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermocycler geneamp pcr system 2700 - by Bioz Stars, 2020-09
    88/100 stars
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