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Illumina Inc gene specific oligonucleotides
Gene Specific Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene specific oligonucleotides/product/Illumina Inc
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
gene specific oligonucleotides - by Bioz Stars, 2020-03
92/100 stars

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Multiplexing:

Article Title: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
Article Snippet: .. Gene-specific oligonucleotides were designed with Tm (melting temperature) differences of less than 1 °C for multiplexing using Primer3 (v. 0.4.0) and tagged with 5′-end tails to allow subsequent for sample indexing (Nextera XT) modifying the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. ..

Amplification:

Article Title: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
Article Snippet: 400 bp genomic segments from 10 meiotic genes of interest (except for a HvFANCM fragment of 479 bp) were identified for PCR amplification from the 96 pooled DNAs (Fig. ). .. Gene-specific oligonucleotides were designed with Tm (melting temperature) differences of less than 1 °C for multiplexing using Primer3 (v. 0.4.0) and tagged with 5′-end tails to allow subsequent for sample indexing (Nextera XT) modifying the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev.

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: .. The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) . .. Hybridization was detected using 1 µg/ml of Cy3-steptavidine (Amersham Biosciences), after which the chips were scanned with an Illumina BeadArray Reader.

In Vitro:

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: Briefly, 500 ng of total RNA were used to conduct in vitro transcription amplification with the Illumina RNA amplification kit (Ambion). .. The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) .

Microarray:

Article Title: Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes
Article Snippet: Paragraph title: DNA microarray and data analysis ... One hundred ng of biotin-labeled cRNA samples were hybridized to Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (Illumina Inc., San Diego, CA, USA) containing over 22,000 transcripts.

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: Paragraph title: DNA microarray ... The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) .

Isolation:

Article Title: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
Article Snippet: Identifying mutations in 400 bp amplicons from multiple meiotic genes 96 DNA samples isolated from three 16 × 16 blocks from the 30 mM EMS treatment (i.e. a total of 768 plants) were used for the following experiment. .. Gene-specific oligonucleotides were designed with Tm (melting temperature) differences of less than 1 °C for multiplexing using Primer3 (v. 0.4.0) and tagged with 5′-end tails to allow subsequent for sample indexing (Nextera XT) modifying the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev.

Sequencing:

Article Title: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
Article Snippet: .. Gene-specific oligonucleotides were designed with Tm (melting temperature) differences of less than 1 °C for multiplexing using Primer3 (v. 0.4.0) and tagged with 5′-end tails to allow subsequent for sample indexing (Nextera XT) modifying the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. ..

Indirect Immunoperoxidase Assay:

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: Transcriptomic Analysis Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Gene ontology and biological pathways and functions were determined using the web-based bioinformatics software Ingenuity Pathway Analysis (IPA; Ingenuity Systems, USA).

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Gene ontology and biological pathways and functions were determined using the web-based bioinformatics software Ingenuity Pathway Analysis (IPA; Ingenuity Systems, USA).

Expressing:

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: .. Transcriptomic Analysis Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Differences in data distribution were analysed using BeadStudio software (Illumina, USA).

Article Title: Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes
Article Snippet: .. One hundred ng of biotin-labeled cRNA samples were hybridized to Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (Illumina Inc., San Diego, CA, USA) containing over 22,000 transcripts. .. Hybridization was detected with 1 μg/mL of Cy3-streptavidine (Amersham Biosciences, Piscataway, NJ, USA) and the chips were scanned with an Illumina BeadArray Reader.

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: .. The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) . .. Hybridization was detected using 1 µg/ml of Cy3-steptavidine (Amersham Biosciences), after which the chips were scanned with an Illumina BeadArray Reader.

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: .. Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Differences in data distribution were analysed using BeadStudio software (Illumina, USA).

Polymerase Chain Reaction:

Article Title: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
Article Snippet: 400 bp genomic segments from 10 meiotic genes of interest (except for a HvFANCM fragment of 479 bp) were identified for PCR amplification from the 96 pooled DNAs (Fig. ). .. Gene-specific oligonucleotides were designed with Tm (melting temperature) differences of less than 1 °C for multiplexing using Primer3 (v. 0.4.0) and tagged with 5′-end tails to allow subsequent for sample indexing (Nextera XT) modifying the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev.

Software:

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: Transcriptomic Analysis Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Differences in data distribution were analysed using BeadStudio software (Illumina, USA).

Article Title: Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes
Article Snippet: One hundred ng of biotin-labeled cRNA samples were hybridized to Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (Illumina Inc., San Diego, CA, USA) containing over 22,000 transcripts. .. Subsequent to image scans, quantile normalization of the data was carried out using Genomestudio software (Illumina Inc., San Diego, CA, USA).

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) . .. Image analysis was conducted using Illumina's Bead Studio software, and the raw data were exported into Microsoft Access .

Article Title: Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System
Article Snippet: Differences in gene expression in subject groups of rBM-MSCs or microglia were examined using the Illumina system (Illumina, USA) in conjunction with Sentrix Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (~22,000 genes, Illumina, USA). .. Differences in data distribution were analysed using BeadStudio software (Illumina, USA).

Chromatin Immunoprecipitation:

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: Microarray analysis was conducted using the Illumina Bead Chip. .. The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) .

Hybridization:

Article Title: Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes
Article Snippet: RNA samples were processed for hybridization according to the manufacturer’s protocol. .. One hundred ng of biotin-labeled cRNA samples were hybridized to Rat-Ref-12-v1 Expression Bead Chips containing gene-specific oligonucleotides (Illumina Inc., San Diego, CA, USA) containing over 22,000 transcripts.

Article Title: ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-?B Activation
Article Snippet: The amplified RNA was then hybridized to Mouse-Ref-v2 Expression Bead Chips containing gene-specific oligonucleotides (∼22,000 genes, Illumina, Inc., San Diego, CA) . .. Hybridization was detected using 1 µg/ml of Cy3-steptavidine (Amersham Biosciences), after which the chips were scanned with an Illumina BeadArray Reader.

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    Illumina Inc gene specific oligonucleotides
    Gene Specific Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene specific oligonucleotides/product/Illumina Inc
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    gene specific oligonucleotides - by Bioz Stars, 2020-03
    92/100 stars
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