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Waters Corporation gen pak fax column
Gen Pak Fax Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gen pak fax column/product/Waters Corporation
Average 88 stars, based on 10 article reviews
Price from $9.99 to $1999.99
gen pak fax column - by Bioz Stars, 2020-08
88/100 stars

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High Performance Liquid Chromatography:

Article Title: Preparation of Fully Synthetic Histone H3 reveals That Acetyl-Lysine 56 Facilitates Protein Binding within Nucleosomes
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. Recombinant histones were expressed and purified as previously described .

Article Title: The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament
Article Snippet: .. For surface plasmon resonance (SPR) analysis, a 5’-biotinylated oligo dT50 was used as ssDNA and for dsDNA 5’-biotinylated 50-mer 5’-TCG AGA GGG TAA ACC ACA-ATT ATT GAT ATA AAA TAG TTT TGG GTA GGC GA was annealed with its complement and purified by HPLC on a Gen-Pak FAX column (Waters). .. D-loop assay substrates were prepared as described [ ]. ϕX174 virion ssDNA and RFI dsDNA were purchased from NEB.

Article Title: ATP-dependent nucleosome unwrapping catalyzed by human RAD51
Article Snippet: .. Following PCR amplification , each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. The 601D-linker and 601E-linker were synthesized by digesting with TspRI in NEB buffer #4 (New England Biolabs) PCR product containing the 601 NPS and TspRI site and PCR product containing the TspRI site and a 90 bp linker DNA.

Article Title: MutL traps MutS at a DNA mismatch
Article Snippet: .. Ligated Cy5–550 bp GT-containing DNA substrates were purified and isolated from PCR and reannealed products by high performance liquid chromatography using a Gen-Pak Fax column (4.6 × 100 mm; Waters). .. For the T-bulge substrate, using E. coli ligase rather than T7 ligase (both from New England Biolabs), incubation at 16 °C over 20 h, and repeated ligase treatments and cleanup using a PCR purification kit (Qiagen) between ligase applications, yielded the best results ( ).

Article Title: Nucleosomes accelerate transcription factor dissociation
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC on a Gen-Pak Fax column (Waters). .. The dinucleosome DNA was synthesized by ligation of two shorter PCR products.

Amplification:

Article Title: Preparation of Fully Synthetic Histone H3 reveals That Acetyl-Lysine 56 Facilitates Protein Binding within Nucleosomes
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. Recombinant histones were expressed and purified as previously described .

Article Title: ATP-dependent nucleosome unwrapping catalyzed by human RAD51
Article Snippet: .. Following PCR amplification , each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. The 601D-linker and 601E-linker were synthesized by digesting with TspRI in NEB buffer #4 (New England Biolabs) PCR product containing the 601 NPS and TspRI site and PCR product containing the TspRI site and a 90 bp linker DNA.

Article Title: Nucleosomes accelerate transcription factor dissociation
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC on a Gen-Pak Fax column (Waters). .. The dinucleosome DNA was synthesized by ligation of two shorter PCR products.

Chromatography:

Article Title: High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity
Article Snippet: .. For DNA footprinting experiments and mobility shift assays, the single strands were 5′ end-labeled by T4 polynucleotide kinase and complementary strands were annealed by a temperature gradient from 90 to 20°C over 2 h. The double-stranded DNA fragments were purified by ionic exchange chromatography using a Gen-Pak FAX column (Waters, 4.6 × 100 mm) with a linear gradient of 40–55% Eluent B (1 M NaCl, 1 mM EDTA, 25 mM Tris–HCl, pH 7.9) for 30 min (Eluent A: 1 mM EDTA, 25 mM Tris–HCl, pH 7.9). .. Electrophoretic mobility shift assays were carried out as described previously ( ).

DNA Footprinting:

Article Title: High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity
Article Snippet: .. For DNA footprinting experiments and mobility shift assays, the single strands were 5′ end-labeled by T4 polynucleotide kinase and complementary strands were annealed by a temperature gradient from 90 to 20°C over 2 h. The double-stranded DNA fragments were purified by ionic exchange chromatography using a Gen-Pak FAX column (Waters, 4.6 × 100 mm) with a linear gradient of 40–55% Eluent B (1 M NaCl, 1 mM EDTA, 25 mM Tris–HCl, pH 7.9) for 30 min (Eluent A: 1 mM EDTA, 25 mM Tris–HCl, pH 7.9). .. Electrophoretic mobility shift assays were carried out as described previously ( ).

Isolation:

Article Title: MutL traps MutS at a DNA mismatch
Article Snippet: .. Ligated Cy5–550 bp GT-containing DNA substrates were purified and isolated from PCR and reannealed products by high performance liquid chromatography using a Gen-Pak Fax column (4.6 × 100 mm; Waters). .. For the T-bulge substrate, using E. coli ligase rather than T7 ligase (both from New England Biolabs), incubation at 16 °C over 20 h, and repeated ligase treatments and cleanup using a PCR purification kit (Qiagen) between ligase applications, yielded the best results ( ).

Purification:

Article Title: Preparation of Fully Synthetic Histone H3 reveals That Acetyl-Lysine 56 Facilitates Protein Binding within Nucleosomes
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. Recombinant histones were expressed and purified as previously described .

Article Title: The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament
Article Snippet: .. For surface plasmon resonance (SPR) analysis, a 5’-biotinylated oligo dT50 was used as ssDNA and for dsDNA 5’-biotinylated 50-mer 5’-TCG AGA GGG TAA ACC ACA-ATT ATT GAT ATA AAA TAG TTT TGG GTA GGC GA was annealed with its complement and purified by HPLC on a Gen-Pak FAX column (Waters). .. D-loop assay substrates were prepared as described [ ]. ϕX174 virion ssDNA and RFI dsDNA were purchased from NEB.

Article Title: Single Molecule Fluorescence Methodologies for Investigating Transcription Factor Binding Kinetics to Nucleosomes and DNA
Article Snippet: .. The ligated DNA molecule with two 601 sequences was purified by HPCL using a Gen-Pak Fax column (Waters). .. Recombinant X. laevis histones were used for reconstituting nucleosomes.

Article Title: ATP-dependent nucleosome unwrapping catalyzed by human RAD51
Article Snippet: .. Following PCR amplification , each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. The 601D-linker and 601E-linker were synthesized by digesting with TspRI in NEB buffer #4 (New England Biolabs) PCR product containing the 601 NPS and TspRI site and PCR product containing the TspRI site and a 90 bp linker DNA.

Article Title: MutL traps MutS at a DNA mismatch
Article Snippet: .. Ligated Cy5–550 bp GT-containing DNA substrates were purified and isolated from PCR and reannealed products by high performance liquid chromatography using a Gen-Pak Fax column (4.6 × 100 mm; Waters). .. For the T-bulge substrate, using E. coli ligase rather than T7 ligase (both from New England Biolabs), incubation at 16 °C over 20 h, and repeated ligase treatments and cleanup using a PCR purification kit (Qiagen) between ligase applications, yielded the best results ( ).

Article Title: Nucleosomes accelerate transcription factor dissociation
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC on a Gen-Pak Fax column (Waters). .. The dinucleosome DNA was synthesized by ligation of two shorter PCR products.

Article Title: High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity
Article Snippet: .. For DNA footprinting experiments and mobility shift assays, the single strands were 5′ end-labeled by T4 polynucleotide kinase and complementary strands were annealed by a temperature gradient from 90 to 20°C over 2 h. The double-stranded DNA fragments were purified by ionic exchange chromatography using a Gen-Pak FAX column (Waters, 4.6 × 100 mm) with a linear gradient of 40–55% Eluent B (1 M NaCl, 1 mM EDTA, 25 mM Tris–HCl, pH 7.9) for 30 min (Eluent A: 1 mM EDTA, 25 mM Tris–HCl, pH 7.9). .. Electrophoretic mobility shift assays were carried out as described previously ( ).

Mobility Shift:

Article Title: High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity
Article Snippet: .. For DNA footprinting experiments and mobility shift assays, the single strands were 5′ end-labeled by T4 polynucleotide kinase and complementary strands were annealed by a temperature gradient from 90 to 20°C over 2 h. The double-stranded DNA fragments were purified by ionic exchange chromatography using a Gen-Pak FAX column (Waters, 4.6 × 100 mm) with a linear gradient of 40–55% Eluent B (1 M NaCl, 1 mM EDTA, 25 mM Tris–HCl, pH 7.9) for 30 min (Eluent A: 1 mM EDTA, 25 mM Tris–HCl, pH 7.9). .. Electrophoretic mobility shift assays were carried out as described previously ( ).

Polymerase Chain Reaction:

Article Title: Preparation of Fully Synthetic Histone H3 reveals That Acetyl-Lysine 56 Facilitates Protein Binding within Nucleosomes
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. Recombinant histones were expressed and purified as previously described .

Article Title: ATP-dependent nucleosome unwrapping catalyzed by human RAD51
Article Snippet: .. Following PCR amplification , each DNA molecule was purified by HPLC with a Gen-Pak Fax column (Waters). .. The 601D-linker and 601E-linker were synthesized by digesting with TspRI in NEB buffer #4 (New England Biolabs) PCR product containing the 601 NPS and TspRI site and PCR product containing the TspRI site and a 90 bp linker DNA.

Article Title: MutL traps MutS at a DNA mismatch
Article Snippet: .. Ligated Cy5–550 bp GT-containing DNA substrates were purified and isolated from PCR and reannealed products by high performance liquid chromatography using a Gen-Pak Fax column (4.6 × 100 mm; Waters). .. For the T-bulge substrate, using E. coli ligase rather than T7 ligase (both from New England Biolabs), incubation at 16 °C over 20 h, and repeated ligase treatments and cleanup using a PCR purification kit (Qiagen) between ligase applications, yielded the best results ( ).

Article Title: Nucleosomes accelerate transcription factor dissociation
Article Snippet: .. Following PCR amplification, each DNA molecule was purified by HPLC on a Gen-Pak Fax column (Waters). .. The dinucleosome DNA was synthesized by ligation of two shorter PCR products.

SPR Assay:

Article Title: The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament
Article Snippet: .. For surface plasmon resonance (SPR) analysis, a 5’-biotinylated oligo dT50 was used as ssDNA and for dsDNA 5’-biotinylated 50-mer 5’-TCG AGA GGG TAA ACC ACA-ATT ATT GAT ATA AAA TAG TTT TGG GTA GGC GA was annealed with its complement and purified by HPLC on a Gen-Pak FAX column (Waters). .. D-loop assay substrates were prepared as described [ ]. ϕX174 virion ssDNA and RFI dsDNA were purchased from NEB.

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    Waters Corporation anion exchange waters gen pak fax column
    Anion Exchange Waters Gen Pak Fax Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anion exchange waters gen pak fax column/product/Waters Corporation
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anion exchange waters gen pak fax column - by Bioz Stars, 2020-08
    85/100 stars
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