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GE Healthcare gel band purification kit
Gel Band Purification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 138 article reviews
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gel band purification kit - by Bioz Stars, 2020-02
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Clone Assay:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: The latter was confirmed in preliminary experiments with cDNA prepared from both NP-specific hybridoma clones of each IgG subclass encoded by V186.2 gene rearrangements (provided by T. Azuma), and spleen cells of mice immunized intraperitoneally with alum-precipitated NP-CG. .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech).

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: DNA polymerases, restriction enzymes, and T4 ligase used for cloning were purchased from New England BioLabs. .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare.

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. Cloned PCR products were sequenced with the BigDye® Terminator Cycle Sequencing Kit v 1.1 (Applied Biosystems).

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: .. The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA). .. To confirm the identity of the PCR products from the T. vaginalis JT isolate, both strands of two clones for each transposon, chosen at random, were sequenced using the BigDye Terminator mix (Applied Biosystems, Foster City, CA) and run on an ABI 377 sequencer (Applied Biosystems, Foster City, CA).

Amplification:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The three independent RT-PCR and ligation reactions were performed and were pooled to create each cDNA library to minimize the bias from PCR amplification.

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: PCR amplification was carried out using the GeneAmp PCR System 9700 thermal cycler (Applied Biosystems, USA). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: The reaction mixtures were overlaid with 50 μl of mineral oil and subjected to amplification in a DNA thermal cycler (Perkin-Elmer model 480; PE Biosystems, Foster City, Calif.). .. Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: Paragraph title: DNA extraction, and amplification and sequencing of the ND4 gene ... These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations.

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: Paragraph title: Soil DNA extraction, 16S rRNA amplification and Ion Torrent sequencing ... Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing.

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: Paragraph title: DNA amplification and sequencing ... The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA).

Article Title: Habitat- and soil-related drivers of the root-associated fungal community of Quercus suber in the Northern Moroccan forest
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl with the primers ITS1FI2 and ITS2 (0.6 μM each), 2 μl of DNA extract, 200 μM of each dNTP, 200 ng/ml BSA, GoTaq® DNA Polymerase (2 units) and 1X Green GoTaq® Reaction Buffer (Promega, Charbonnieres, France), with the following cycling conditions: 95°C for 15 min; 30 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; a final elongation step at 72°C for 5 min. To increase richness recovery and to limit PCR biases, three PCR replicates per sample were pooled and purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Velizy-Villacoublay, France) following manufacturer’s guidelines. .. All amplicon products were subjected to paired-end Illumina MiSeq sequencing (2×300 bp) by Molecular Research LP (MR DNA, TX, USA).

Synthesized:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: First-strand cDNA was synthesized with specific primers, Cγ 5-GTGT/CGCACACC/TG/ACTGGACAGGGA/ CTCCAG/TAG, Cα 5-CAGCGGCCGCGGCAGCTGGGA, Cμ 5-GCCAGGCAGCCCATGGCCACC, or with an oligo-dT primer by use of a Superscript Kit (GIBCO BRL). .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech).

Article Title: Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum ▿ ▿ †
Article Snippet: Oligonucleotides used to generate bipartite PCR fragments and to investigate the targeting pattern were synthesized by Sigma-Aldrich (United Kingdom). .. PCR products were purified from agarose gels using Illustra DNA and a gel band purification kit (GE Healthcare).

Picogreen Assay:

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

TA Cloning:

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: .. The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA). .. To confirm the identity of the PCR products from the T. vaginalis JT isolate, both strands of two clones for each transposon, chosen at random, were sequenced using the BigDye Terminator mix (Applied Biosystems, Foster City, CA) and run on an ABI 377 sequencer (Applied Biosystems, Foster City, CA).

Electrophoresis:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: PCR products were separated by electrophoresis on 1.5% (wt/vol) agarose gel (Gibco BRL, France) stained with ethidium bromide (0.5 mg/ml). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: In the Northern blot analysis, approximately 20 μg of total RNA were resolved by electrophoresis on a formaldehyde agarose gel, transferred to a nylon membrane (Sambrook and Russell, ), and hybridized with digoxigenin (DIG)-labeled DNA probes (DIG High Prime DNA Labeling and Detection Starter Kit I, Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer's protocol. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Ten microliters of each reaction mixture was analyzed by electrophoresis in a 1.0% agarose gel and visualized after staining with ethidium bromide. .. Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: .. Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

cDNA Library Assay:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The three independent RT-PCR and ligation reactions were performed and were pooled to create each cDNA library to minimize the bias from PCR amplification.

Expressing:

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: The expression patterns of selected differentially expressed candidate proteins were indirectly confirmed by Northern blot or RT-PCR analysis. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Transformation Assay:

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: Standard methods were used for extraction of the plasmid DNA, DNA digestion with restriction endonucleases, ligation, gel electrophoresis, and transformation of E. coli cells ( ). .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol.

Hybridization:

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: Ligase chain reaction (LCR) and primer hybridization reactions were performed as published before. .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions.

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: The hybridization results were detected with an enzyme immunoassay and imaged with the Gel Doc™ EQ gel documentation system installed with the Quantity One analysis software (version 4.5.0) (Bio-Rad, Hercules, CA). .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Sequencing:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: Paragraph title: Sequence Analysis of VDJ DNA Segments. ... PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech).

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: Paragraph title: DNA extraction and molecular identification by 16S rRNA, pheS and recA genes sequencing ... The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum ▿ ▿ †
Article Snippet: Paragraph title: Functional analysis of MpaC. (i) Oligonucleotides, PCR, and sequencing analyses. ... PCR products were purified from agarose gels using Illustra DNA and a gel band purification kit (GE Healthcare).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Paragraph title: PCR and DNA sequence determination. ... Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. Cloned PCR products were sequenced with the BigDye® Terminator Cycle Sequencing Kit v 1.1 (Applied Biosystems).

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: Paragraph title: DNA extraction, and amplification and sequencing of the ND4 gene ... These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations.

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol. .. Nucleotide sequencing was performed with an ABI PRISM model 310 sequencer (Applied Biosystems).

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: Paragraph title: DNA amplification and sequencing ... The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA).

Article Title: Habitat- and soil-related drivers of the root-associated fungal community of Quercus suber in the Northern Moroccan forest
Article Snippet: Paragraph title: DNA extraction, ITS amplification and Illumina Miseq sequencing ... The amplification reaction was performed in a final volume of 25 μl with the primers ITS1FI2 and ITS2 (0.6 μM each), 2 μl of DNA extract, 200 μM of each dNTP, 200 ng/ml BSA, GoTaq® DNA Polymerase (2 units) and 1X Green GoTaq® Reaction Buffer (Promega, Charbonnieres, France), with the following cycling conditions: 95°C for 15 min; 30 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; a final elongation step at 72°C for 5 min. To increase richness recovery and to limit PCR biases, three PCR replicates per sample were pooled and purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Velizy-Villacoublay, France) following manufacturer’s guidelines.

Article Title: Genetic Basis of Evolutionary Adaptation by Escherichia coli to Stressful Cycles of Freezing, Thawing and Growth
Article Snippet: Paragraph title: DNA extractions, PCR, and sequencing: ... PCR products were purified using a GFX PCR DNA and gel band purification kit (Amersham Biosciences, Arlington Heights, IL).

Ligation:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The three independent RT-PCR and ligation reactions were performed and were pooled to create each cDNA library to minimize the bias from PCR amplification.

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: Standard methods were used for extraction of the plasmid DNA, DNA digestion with restriction endonucleases, ligation, gel electrophoresis, and transformation of E. coli cells ( ). .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol.

Northern Blot:

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: In the Northern blot analysis, approximately 20 μg of total RNA were resolved by electrophoresis on a formaldehyde agarose gel, transferred to a nylon membrane (Sambrook and Russell, ), and hybridized with digoxigenin (DIG)-labeled DNA probes (DIG High Prime DNA Labeling and Detection Starter Kit I, Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer's protocol. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Generated:

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: Point mutations were generated by QuickChange Site-Directed mutagenesis (Stratagene, La Jolla, USA). .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions.

DNA Sequencing:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy). .. DNA sequencing reactions were carried out by MWG Biotech AG (Ebersberg, Germany) using both, forward and reverse, primers.

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare. .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare.

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer. .. DNA sequencing on an ABI model 377 PRISM automated DNA sequencer was performed by the core laboratory at the Massachusetts General Hospital (Boston).

DNA Labeling:

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: In the Northern blot analysis, approximately 20 μg of total RNA were resolved by electrophoresis on a formaldehyde agarose gel, transferred to a nylon membrane (Sambrook and Russell, ), and hybridized with digoxigenin (DIG)-labeled DNA probes (DIG High Prime DNA Labeling and Detection Starter Kit I, Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer's protocol. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The three independent RT-PCR and ligation reactions were performed and were pooled to create each cDNA library to minimize the bias from PCR amplification.

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: The expression patterns of selected differentially expressed candidate proteins were indirectly confirmed by Northern blot or RT-PCR analysis. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: .. Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

Recombinant:

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: Recombinant plasmid DNA was purified with a QIAprep Kit from Qiagen or with a E.Z.N.A. .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare.

DNA Extraction:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: Paragraph title: DNA extraction and molecular identification by 16S rRNA, pheS and recA genes sequencing ... The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: Paragraph title: DNA extraction, and amplification and sequencing of the ND4 gene ... These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations.

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: Paragraph title: Soil DNA extraction, 16S rRNA amplification and Ion Torrent sequencing ... Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing.

Article Title: Habitat- and soil-related drivers of the root-associated fungal community of Quercus suber in the Northern Moroccan forest
Article Snippet: Paragraph title: DNA extraction, ITS amplification and Illumina Miseq sequencing ... The amplification reaction was performed in a final volume of 25 μl with the primers ITS1FI2 and ITS2 (0.6 μM each), 2 μl of DNA extract, 200 μM of each dNTP, 200 ng/ml BSA, GoTaq® DNA Polymerase (2 units) and 1X Green GoTaq® Reaction Buffer (Promega, Charbonnieres, France), with the following cycling conditions: 95°C for 15 min; 30 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; a final elongation step at 72°C for 5 min. To increase richness recovery and to limit PCR biases, three PCR replicates per sample were pooled and purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Velizy-Villacoublay, France) following manufacturer’s guidelines.

Article Title: Molecular Properties of Salmonella enterica Serotype Paratyphi B Distinguish between Its Systemic and Its Enteric Pathovars
Article Snippet: Paragraph title: DNA isolation and PCR. ... PCR-generated DNA probes were purified by using GFX PCR DNA and a gel band purification kit (Amersham Biosciences, Little Chalfont, United Kingdom).

Nucleic Acid Electrophoresis:

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: Standard methods were used for extraction of the plasmid DNA, DNA digestion with restriction endonucleases, ligation, gel electrophoresis, and transformation of E. coli cells ( ). .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol.

Mutagenesis:

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: Point mutations were generated by QuickChange Site-Directed mutagenesis (Stratagene, La Jolla, USA). .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions.

Isolation:

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: Plasmid isolation and PCR product purification was performed with NucleoSpin Plasmid and NucleoSpin Gel and PCR Clean-up kits (Macherey Nagel), E.Z.N.A. .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions.

Negative Control:

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: A negative control was used in all PCR reactions. .. These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations.

Purification:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The purified fragments were cloned into the PCR-Script™ Amp cloning vector (Stratagene) according to the manufacturer's instructions.

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy). .. DNA sequencing reactions were carried out by MWG Biotech AG (Ebersberg, Germany) using both, forward and reverse, primers.

Article Title: Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum ▿ ▿ †
Article Snippet: .. PCR products were purified from agarose gels using Illustra DNA and a gel band purification kit (GE Healthcare). .. Sequencing of PCR fragments obtained from transformants was performed by MWG Biotech.

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions. ..

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ). .. RT-PCR was performed with the ReverTra Ace Kit (Toyobo, Osaka, Japan) and HotStarTaq DNA polymerase (Qiagen, Chatsworth, CA) as described previously (Fu et al., ).

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare. .. DNA sequencing was performed by Beckman Coulter Genomics (Danvers, MA) and Eton Bioscience (Charlestown, MA).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: .. Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer. .. Purified fragments were ligated into the Promega pGEM T-easy vector (Promega, Madison, Wis.).

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: .. Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: .. These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations. .. Subsequently, they were sequenced in an automated MegaBACE 1000 Analysis System sequencer (GE Healthcare, UK).

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol. .. Nucleotide sequencing was performed with an ABI PRISM model 310 sequencer (Applied Biosystems).

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: .. The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA). .. To confirm the identity of the PCR products from the T. vaginalis JT isolate, both strands of two clones for each transposon, chosen at random, were sequenced using the BigDye Terminator mix (Applied Biosystems, Foster City, CA) and run on an ABI 377 sequencer (Applied Biosystems, Foster City, CA).

Article Title: Habitat- and soil-related drivers of the root-associated fungal community of Quercus suber in the Northern Moroccan forest
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl with the primers ITS1FI2 and ITS2 (0.6 μM each), 2 μl of DNA extract, 200 μM of each dNTP, 200 ng/ml BSA, GoTaq® DNA Polymerase (2 units) and 1X Green GoTaq® Reaction Buffer (Promega, Charbonnieres, France), with the following cycling conditions: 95°C for 15 min; 30 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; a final elongation step at 72°C for 5 min. To increase richness recovery and to limit PCR biases, three PCR replicates per sample were pooled and purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Velizy-Villacoublay, France) following manufacturer’s guidelines. .. All amplicon products were subjected to paired-end Illumina MiSeq sequencing (2×300 bp) by Molecular Research LP (MR DNA, TX, USA).

Article Title: Genetic Basis of Evolutionary Adaptation by Escherichia coli to Stressful Cycles of Freezing, Thawing and Growth
Article Snippet: .. PCR products were purified using a GFX PCR DNA and gel band purification kit (Amersham Biosciences, Arlington Heights, IL). .. Purified PCR products were sequenced by the dideoxy chain termination method ( ).

Polymerase Chain Reaction:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The purified fragments were cloned into the PCR-Script™ Amp cloning vector (Stratagene) according to the manufacturer's instructions.

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy). .. DNA sequencing reactions were carried out by MWG Biotech AG (Ebersberg, Germany) using both, forward and reverse, primers.

Article Title: Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum ▿ ▿ †
Article Snippet: .. PCR products were purified from agarose gels using Illustra DNA and a gel band purification kit (GE Healthcare). .. Sequencing of PCR fragments obtained from transformants was performed by MWG Biotech.

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions. ..

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ). .. RT-PCR was performed with the ReverTra Ace Kit (Toyobo, Osaka, Japan) and HotStarTaq DNA polymerase (Qiagen, Chatsworth, CA) as described previously (Fu et al., ).

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare. .. DNA sequencing was performed by Beckman Coulter Genomics (Danvers, MA) and Eton Bioscience (Charlestown, MA).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Paragraph title: PCR and DNA sequence determination. ... Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: .. Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: .. These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations. .. Subsequently, they were sequenced in an automated MegaBACE 1000 Analysis System sequencer (GE Healthcare, UK).

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol. .. Nucleotide sequencing was performed with an ABI PRISM model 310 sequencer (Applied Biosystems).

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: .. The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA). .. To confirm the identity of the PCR products from the T. vaginalis JT isolate, both strands of two clones for each transposon, chosen at random, were sequenced using the BigDye Terminator mix (Applied Biosystems, Foster City, CA) and run on an ABI 377 sequencer (Applied Biosystems, Foster City, CA).

Article Title: Habitat- and soil-related drivers of the root-associated fungal community of Quercus suber in the Northern Moroccan forest
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl with the primers ITS1FI2 and ITS2 (0.6 μM each), 2 μl of DNA extract, 200 μM of each dNTP, 200 ng/ml BSA, GoTaq® DNA Polymerase (2 units) and 1X Green GoTaq® Reaction Buffer (Promega, Charbonnieres, France), with the following cycling conditions: 95°C for 15 min; 30 cycles of 95°C for 30 s, 58°C for 30 s, 72°C for 30 s; a final elongation step at 72°C for 5 min. To increase richness recovery and to limit PCR biases, three PCR replicates per sample were pooled and purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Velizy-Villacoublay, France) following manufacturer’s guidelines. .. All amplicon products were subjected to paired-end Illumina MiSeq sequencing (2×300 bp) by Molecular Research LP (MR DNA, TX, USA).

Article Title: Genetic Basis of Evolutionary Adaptation by Escherichia coli to Stressful Cycles of Freezing, Thawing and Growth
Article Snippet: .. PCR products were purified using a GFX PCR DNA and gel band purification kit (Amersham Biosciences, Arlington Heights, IL). .. Purified PCR products were sequenced by the dideoxy chain termination method ( ).

Article Title: Molecular Properties of Salmonella enterica Serotype Paratyphi B Distinguish between Its Systemic and Its Enteric Pathovars
Article Snippet: .. PCR-generated DNA probes were purified by using GFX PCR DNA and a gel band purification kit (Amersham Biosciences, Little Chalfont, United Kingdom). .. Restriction fragment length polymorphisms (RFLP) of PCR products were analyzed as described earlier ( ).

Staining:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: PCR products were separated by electrophoresis on 1.5% (wt/vol) agarose gel (Gibco BRL, France) stained with ethidium bromide (0.5 mg/ml). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Ten microliters of each reaction mixture was analyzed by electrophoresis in a 1.0% agarose gel and visualized after staining with ethidium bromide. .. Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Article Snippet: PCR products were deposited on 1% agarose gels, stained with ethidium bromide and analyzed under UV light. .. These were then purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare, UK) according to manufacturer's recommendations.

Nested PCR:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: The primers used for the nested PCR were as follows: as a sense-primer, V186.2 -specific primers were used ( ); as an antisense primer, Cγ 5-CAGGGGCCAGTGGATAGACAGATG, Cα 5-GTGAATTCAGGCGGCCGCTTA, or Cμ 5-AGGGGGCTCTCGCAGGAGACG was used. .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech).

Mouse Assay:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: The latter was confirmed in preliminary experiments with cDNA prepared from both NP-specific hybridoma clones of each IgG subclass encoded by V186.2 gene rearrangements (provided by T. Azuma), and spleen cells of mice immunized intraperitoneally with alum-precipitated NP-CG. .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech).

Chromatin Immunoprecipitation:

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

Plasmid Preparation:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The purified fragments were cloned into the PCR-Script™ Amp cloning vector (Stratagene) according to the manufacturer's instructions.

Article Title: Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1
Article Snippet: .. Plasmid Mini Kit (Omega Bio-Tek), or illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions. ..

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: Plasmid DNA Mini Kit I from Omega Bio-Tek. .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare.

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer. .. Purified fragments were ligated into the Promega pGEM T-easy vector (Promega, Madison, Wis.).

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

Article Title: Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase †
Article Snippet: Standard methods were used for extraction of the plasmid DNA, DNA digestion with restriction endonucleases, ligation, gel electrophoresis, and transformation of E. coli cells ( ). .. Purification of the PCR-amplified DNA fragments was done with GFX PCR DNA and Gel Band purification kit (Amersham Biosciences) according to the manufacturer's protocol.

Software:

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: The hybridization results were detected with an enzyme immunoassay and imaged with the Gel Doc™ EQ gel documentation system installed with the Quantity One analysis software (version 4.5.0) (Bio-Rad, Hercules, CA). .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer. .. DNA sequence analysis was performed on a Macintosh G3 computer using the MacVector 6.5 software package (Oxford Molecular, Campbell, Calif.).

Enzyme-linked Immunosorbent Assay:

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: The hybridization results were detected with an enzyme immunoassay and imaged with the Gel Doc™ EQ gel documentation system installed with the Quantity One analysis software (version 4.5.0) (Bio-Rad, Hercules, CA). .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Multiplex Assay:

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: Primers for each sample contained unique multiplex identifier codes and adapter sequences for Ion Torrent sequencing. .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing.

Agarose Gel Electrophoresis:

Article Title: Isotype-specific Selection of High Affinity Memory B Cells in Nasal-associated Lymphoid Tissue
Article Snippet: .. PCR products were size-fractionated by agarose gel electrophoresis and purified with GFX™ PCR DNA and a Gel Band Purification kit (Amersham Pharmacia Biotech). .. The purified fragments were cloned into the PCR-Script™ Amp cloning vector (Stratagene) according to the manufacturer's instructions.

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: PCR products were separated by electrophoresis on 1.5% (wt/vol) agarose gel (Gibco BRL, France) stained with ethidium bromide (0.5 mg/ml). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: OMICS in Ecology: Systems Level Analyses of Halobacterium salinarum Reveal Large-scale Temperature-Mediated Changes and a Requirement of CctA for Thermotolerance
Article Snippet: In the Northern blot analysis, approximately 20 μg of total RNA were resolved by electrophoresis on a formaldehyde agarose gel, transferred to a nylon membrane (Sambrook and Russell, ), and hybridized with digoxigenin (DIG)-labeled DNA probes (DIG High Prime DNA Labeling and Detection Starter Kit I, Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer's protocol. .. The DNA probes were prepared by PCR using HotStarTaq DNA polymerase (Qiagen, Valencia, CA) and purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ).

Article Title: Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
Article Snippet: Ten microliters of each reaction mixture was analyzed by electrophoresis in a 1.0% agarose gel and visualized after staining with ethidium bromide. .. Bands of interest were excised from the gel and purified by using a gel band purification kit (Amersham Pharmacia Biotech) in accordance with the recommendations of the manufacturer.

Article Title: Intron RNA editing is essential for splicing in plant mitochondria
Article Snippet: .. Quantization of RNA editing To determine the profile and the rate of C-to-U conversion in RT–PCR products, the precursor and mature PCR bands were excised from agarose gel after separation by electrophoresis and purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). .. The purified fragments were ligated into pGEM-T easy vector as described by the manufacturer (Promega).

Article Title: Molecular Properties of Salmonella enterica Serotype Paratyphi B Distinguish between Its Systemic and Its Enteric Pathovars
Article Snippet: Chromosomal DNA isolation, DNA cleavage with restriction enzymes, and agarose gel electrophoresis were performed as described by Prager et al. ( ). .. PCR-generated DNA probes were purified by using GFX PCR DNA and a gel band purification kit (Amersham Biosciences, Little Chalfont, United Kingdom).

Functional Assay:

Article Title: Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum ▿ ▿ †
Article Snippet: Paragraph title: Functional analysis of MpaC. (i) Oligonucleotides, PCR, and sequencing analyses. ... PCR products were purified from agarose gels using Illustra DNA and a gel band purification kit (GE Healthcare).

Spectrophotometry:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: The concentration and purity of DNA was assessed by a NanoDrop® ND-1000 Spectrophotometer (Thermo Fisher Scientific Inc.). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Genetic Basis of Evolutionary Adaptation by Escherichia coli to Stressful Cycles of Freezing, Thawing and Growth
Article Snippet: DNA was extracted from cultures grown overnight in LB medium using the QIAGEN (Valencia, CA) genomic-tip 100/G kit and quantified using a spectrophotometer. .. PCR products were purified using a GFX PCR DNA and gel band purification kit (Amersham Biosciences, Arlington Heights, IL).

Produced:

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Ion Torrent sequencing has been shown to produce microbial community profiles that are highly comparable to those produced by 454 sequencing ( ).

Concentration Assay:

Article Title: Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization
Article Snippet: The concentration and purity of DNA was assessed by a NanoDrop® ND-1000 Spectrophotometer (Thermo Fisher Scientific Inc.). .. The amplicons were eluted from gel and purified by the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences, Milan, Italy).

Article Title: Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
Article Snippet: .. Amplicons were gel purified using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Piscataway, NJ, USA), and then quantified using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Burlington, Ontario, Canada), pooled in equimolar ratios, and diluted to a concentration of 5 × 107 molecules for sequencing. .. Sequencing was conducted on an Ion Torrent Personal Genome Machine using the Ion Xpress Template Kit (Life Technologies, Carlsbad, CA, USA) and the Ion 314 chip (Life Technologies) following the manufacturer's protocols.

Article Title: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Article Snippet: PCR was done in a volume of 25 μl with 0.5U of Taq DNA polymerase in 1× polymerase buffer, 10 μM of each primer, a 200 μM concentration of each dNTP and 1.5 mM MgCl2. .. The solutions were heated to 94°C for 2 min, and followed by 35 cycles of denaturation (94°C for 1 min), annealing (60°C for 2 min), and extension (72°C for 1 min), followed by a final extension at 72°C for 10 min. PCR products with the expected size were excised from 1% agarose gels, purified using GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Little Chalfont, UK), and cloned using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA).

Gel Extraction:

Article Title: Discovery of a diazo-forming enzyme in cremeomycin biosynthesis
Article Snippet: .. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification kit from GE Healthcare. .. DNA sequencing was performed by Beckman Coulter Genomics (Danvers, MA) and Eton Bioscience (Charlestown, MA).

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    GE Healthcare gel band purificatoin kit
    Gel Band Purificatoin Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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