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GE Healthcare gel band purification kit
Gel Band Purification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel band purification kit/product/GE Healthcare
Average 99 stars, based on 136 article reviews
Price from $9.99 to $1999.99
gel band purification kit - by Bioz Stars, 2020-09
99/100 stars

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Clone Assay:

Article Title: Characterization of Three Novel Adhesins of Leptospira interrogans
Article Snippet: .. Gel-purified PCR fragments (Illustra GFX PCR DNA and Gel band purification kit; GE Healthcare Bio-Sciences Corp, Piscataway, NJ) were subcloned into the pGEM-T easy vector (Promega, Prodimol, Minas Gerais, Brazil) and cloned into the pAE expression vector at Xho I and Hind III (LIC11009 and LIC11975) or Bam HI and Hind III restriction sites (LIC11360). .. The pAE vector enables expression of recombinant proteins with a minimal 6XHis-tag at the N-terminus.

Amplification:

Article Title: Expression of multiple forms of 3'-end variant CCK2 receptor mRNAs in human pancreatic adenocarcinomas
Article Snippet: .. Amplicons were purified using the GFX PCR DNA gel band purification kit (GE-Health Care, Uppsala, Sweden), followed by a second PCR using 1 μl purified amplicon, primer 3 and 4 (CCKBRseq.se1 and CCKBR.as2), and PCR amplification condition No. 3. .. To confirm the expression of CCK2i4svR mRNA, a semi-nested PCR assays were carried out.

DNA Purification:

Article Title: Associations between infant fungal and bacterial dysbiosis and childhood atopic wheeze in a nonindustrialized setting
Article Snippet: .. Amplicons displaying bands at approximately 250 bp were purified with the Illustra GFX PCR DNA Purification Kit (GE Healthcare, Fairfield, Conn). .. Purified samples were diluted 1:50 and quantified with PicoGreen (Invitrogen, Carlsbad, Calif) in the TECAN M200 (excitation at 480 nm and emission at 520 nm).

Purification:

Article Title: The proposed new species, cacao red vein virus, and three previously recognized badnavirus species are associated with cacao swollen shoot disease
Article Snippet: .. Bands ~7 kbp in size were excised and gel-purified using the illustra GFX PCR and DNA Gel Band Purification kit (GE Healthcare Bio-Sciences), according to manufacturer’s instructions. .. The concentration of DNA was determined using the Nanodrop 2000 UV–vis spectrophotometer (Thermo Scientific).

Article Title: Characterization of Three Novel Adhesins of Leptospira interrogans
Article Snippet: .. Gel-purified PCR fragments (Illustra GFX PCR DNA and Gel band purification kit; GE Healthcare Bio-Sciences Corp, Piscataway, NJ) were subcloned into the pGEM-T easy vector (Promega, Prodimol, Minas Gerais, Brazil) and cloned into the pAE expression vector at Xho I and Hind III (LIC11009 and LIC11975) or Bam HI and Hind III restriction sites (LIC11360). .. The pAE vector enables expression of recombinant proteins with a minimal 6XHis-tag at the N-terminus.

Article Title: Expression of multiple forms of 3'-end variant CCK2 receptor mRNAs in human pancreatic adenocarcinomas
Article Snippet: .. Amplicons were purified using the GFX PCR DNA gel band purification kit (GE-Health Care, Uppsala, Sweden), followed by a second PCR using 1 μl purified amplicon, primer 3 and 4 (CCKBRseq.se1 and CCKBR.as2), and PCR amplification condition No. 3. .. To confirm the expression of CCK2i4svR mRNA, a semi-nested PCR assays were carried out.

Article Title: Identification of deep intronic individual variants in patients with hemophilia A by next‐generation sequencing of the whole factor VIII gene. Identification of deep intronic individual variants in patients with hemophilia A by next‐generation sequencing of the whole factor VIII gene
Article Snippet: .. The PCR fragments were purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare UK Ltd. Little Chalfont, Buckinghamshire, UK), and mixed in equimolar amounts. .. The DNA library was prepared by fragmentation using a Nextera XT DNA sample preparation kit (Illumina Inc., San Diego, CA, USA).

Article Title: Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection
Article Snippet: .. The PCR products obtained were purified using the GFX PCR DNA gel band purification kit (GE Healthcare), to be sequenced as described above. .. The sequence generated was aligned with the sequences of M. tuberculosis H37Rv ( ) and CDC1551 ( ).

Article Title: Associations between infant fungal and bacterial dysbiosis and childhood atopic wheeze in a nonindustrialized setting
Article Snippet: .. Amplicons displaying bands at approximately 250 bp were purified with the Illustra GFX PCR DNA Purification Kit (GE Healthcare, Fairfield, Conn). .. Purified samples were diluted 1:50 and quantified with PicoGreen (Invitrogen, Carlsbad, Calif) in the TECAN M200 (excitation at 480 nm and emission at 520 nm).

Article Title: Prophagic DNA Fragments in Streptococcus agalactiae Strains and Association with Neonatal Meningitis
Article Snippet: .. Ten fragments meeting these criteria were selected, extracted, and purified from the agarose plugs, using the GFXTM PCR DNA gel band purification kit (Amersham Biosciences, Orsay France). .. Each DNA fragment was inserted into pCR 2.1 (TA cloning kit; Invitrogen, Cergy Pontoise, France).

Expressing:

Article Title: Characterization of Three Novel Adhesins of Leptospira interrogans
Article Snippet: .. Gel-purified PCR fragments (Illustra GFX PCR DNA and Gel band purification kit; GE Healthcare Bio-Sciences Corp, Piscataway, NJ) were subcloned into the pGEM-T easy vector (Promega, Prodimol, Minas Gerais, Brazil) and cloned into the pAE expression vector at Xho I and Hind III (LIC11009 and LIC11975) or Bam HI and Hind III restriction sites (LIC11360). .. The pAE vector enables expression of recombinant proteins with a minimal 6XHis-tag at the N-terminus.

Polymerase Chain Reaction:

Article Title: The proposed new species, cacao red vein virus, and three previously recognized badnavirus species are associated with cacao swollen shoot disease
Article Snippet: .. Bands ~7 kbp in size were excised and gel-purified using the illustra GFX PCR and DNA Gel Band Purification kit (GE Healthcare Bio-Sciences), according to manufacturer’s instructions. .. The concentration of DNA was determined using the Nanodrop 2000 UV–vis spectrophotometer (Thermo Scientific).

Article Title: Characterization of Three Novel Adhesins of Leptospira interrogans
Article Snippet: .. Gel-purified PCR fragments (Illustra GFX PCR DNA and Gel band purification kit; GE Healthcare Bio-Sciences Corp, Piscataway, NJ) were subcloned into the pGEM-T easy vector (Promega, Prodimol, Minas Gerais, Brazil) and cloned into the pAE expression vector at Xho I and Hind III (LIC11009 and LIC11975) or Bam HI and Hind III restriction sites (LIC11360). .. The pAE vector enables expression of recombinant proteins with a minimal 6XHis-tag at the N-terminus.

Article Title: Expression of multiple forms of 3'-end variant CCK2 receptor mRNAs in human pancreatic adenocarcinomas
Article Snippet: .. Amplicons were purified using the GFX PCR DNA gel band purification kit (GE-Health Care, Uppsala, Sweden), followed by a second PCR using 1 μl purified amplicon, primer 3 and 4 (CCKBRseq.se1 and CCKBR.as2), and PCR amplification condition No. 3. .. To confirm the expression of CCK2i4svR mRNA, a semi-nested PCR assays were carried out.

Article Title: Identification of deep intronic individual variants in patients with hemophilia A by next‐generation sequencing of the whole factor VIII gene. Identification of deep intronic individual variants in patients with hemophilia A by next‐generation sequencing of the whole factor VIII gene
Article Snippet: .. The PCR fragments were purified using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare UK Ltd. Little Chalfont, Buckinghamshire, UK), and mixed in equimolar amounts. .. The DNA library was prepared by fragmentation using a Nextera XT DNA sample preparation kit (Illumina Inc., San Diego, CA, USA).

Article Title: Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection
Article Snippet: .. The PCR products obtained were purified using the GFX PCR DNA gel band purification kit (GE Healthcare), to be sequenced as described above. .. The sequence generated was aligned with the sequences of M. tuberculosis H37Rv ( ) and CDC1551 ( ).

Article Title: Associations between infant fungal and bacterial dysbiosis and childhood atopic wheeze in a nonindustrialized setting
Article Snippet: .. Amplicons displaying bands at approximately 250 bp were purified with the Illustra GFX PCR DNA Purification Kit (GE Healthcare, Fairfield, Conn). .. Purified samples were diluted 1:50 and quantified with PicoGreen (Invitrogen, Carlsbad, Calif) in the TECAN M200 (excitation at 480 nm and emission at 520 nm).

Article Title: Prophagic DNA Fragments in Streptococcus agalactiae Strains and Association with Neonatal Meningitis
Article Snippet: .. Ten fragments meeting these criteria were selected, extracted, and purified from the agarose plugs, using the GFXTM PCR DNA gel band purification kit (Amersham Biosciences, Orsay France). .. Each DNA fragment was inserted into pCR 2.1 (TA cloning kit; Invitrogen, Cergy Pontoise, France).

Plasmid Preparation:

Article Title: Characterization of Three Novel Adhesins of Leptospira interrogans
Article Snippet: .. Gel-purified PCR fragments (Illustra GFX PCR DNA and Gel band purification kit; GE Healthcare Bio-Sciences Corp, Piscataway, NJ) were subcloned into the pGEM-T easy vector (Promega, Prodimol, Minas Gerais, Brazil) and cloned into the pAE expression vector at Xho I and Hind III (LIC11009 and LIC11975) or Bam HI and Hind III restriction sites (LIC11360). .. The pAE vector enables expression of recombinant proteins with a minimal 6XHis-tag at the N-terminus.

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    GE Healthcare gel band purificatoin kit
    Gel Band Purificatoin Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel band purificatoin kit/product/GE Healthcare
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gel band purificatoin kit - by Bioz Stars, 2020-09
    99/100 stars
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