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Thermo Fisher gc agar
Gc Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gc agar/product/Thermo Fisher
Average 99 stars, based on 16 article reviews
Price from $9.99 to $1999.99
gc agar - by Bioz Stars, 2020-03
99/100 stars

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Related Articles

Diagnostic Assay:

Article Title: Monitoring Antimicrobial Susceptibility of Neisseria gonorrhoeae Isolated from Bangladesh during 1997-2006: Emergence and Pattern of Drug-resistant Isolates
Article Snippet: .. Ten microlitre of the bacterial suspension (1×104 cfu) was spotted on GC agar (Oxoid Ltd, Basingstoke, Hampshaire, UK) plates as recommended by the WHO, containing two-fold serial dilution of antimicrobial agents using a multi-point inoculator (Mast Diagnostic Ltd., SCAN 114). .. The quality control of the MIC test was ensured by including seven N. gonorrhoeae reference strains WHO A, B, C, D, E, G, and H with known MICs in each test, by repeating each test three times and by participating in the WPRO/SEARO GASP (Western Pacific Region Office/South-East Asia Regional Office/Gonococcal Antimicrobial Surveillance Programme) external quality-assurance programme (our data were in agreement with expected results for all antimicrobial agents during the study period).

Amplification:

Article Title: Microbiological Analysis from a Phase 2 Randomized Study in Adults Evaluating Single Oral Doses of Gepotidacin in the Treatment of Uncomplicated Urogenital Gonorrhea Caused by Neisseria gonorrhoeae
Article Snippet: The PCR primers for the amplification of N. gonorrhoeae gyrA and parC ). .. To assess FoR, 2 separate studies were performed by GlaxoSmithKline (Collegeville, PA) using Oxoid GC agar base containing BB BBL IsovitaleX enrichment in study 1 and Remel agar base plus hemoglobin and BB BBL IsovitaleX Enrichment in study 2.

Incubation:

Article Title: Mutations in Neisseria gonorrhoeae grown in sub-lethal concentrations of monocaprin do not confer resistance
Article Snippet: N . gonorrhoeae strain NCCP11945 was grown in three conditions: on standard GC agar (GC base (Oxoid) with Kellogg’s supplements [ ]); on GC agar with sub-lethal monocaprin (Sigma-Aldrich; C10:0MG; 125 μM); and on GC agar with sub-lethal myristic acid (Sigma-Aldrich; C14:0; 62.5 μM). .. Minimally passaged N . gonorrhoeae strain NCCP11945 received from the sequencing project [ ] was incubated at 37°C, 5% CO2 for 48 hours.

Article Title: Microbiological Analysis from a Phase 2 Randomized Study in Adults Evaluating Single Oral Doses of Gepotidacin in the Treatment of Uncomplicated Urogenital Gonorrhea Caused by Neisseria gonorrhoeae
Article Snippet: N. gonorrhoeae was subcultured from a frozen stock onto chocolate II agar plates and incubated at 35°C for 18 h with 5% carbon dioxide. .. To assess FoR, 2 separate studies were performed by GlaxoSmithKline (Collegeville, PA) using Oxoid GC agar base containing BB BBL IsovitaleX enrichment in study 1 and Remel agar base plus hemoglobin and BB BBL IsovitaleX Enrichment in study 2.

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: The agar plates were incubated at 37°C in a 5% CO2 atmosphere for 48 h (mobile transport system). .. In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid).

Article Title: DNA Methylation Assessed by SMRT Sequencing Is Linked to Mutations in Neisseria meningitidis Isolates
Article Snippet: For genomic DNA preparation, strains were grown on supplemented GC agar base (Oxoid) plates for 20–24 hours in 5% CO2 at 37°C. .. Single colonies were transferred into liquid Brain Heart Infusion (BactoTM) medium and again incubated overnight in 5% CO2 at 37°C.

Article Title: Mutations in Neisseria gonorrhoeae grown in sub-lethal concentrations of monocaprin do not confer resistance
Article Snippet: Bacterial growth and experimental evolution N . gonorrhoeae strain NCCP11945 was grown in three conditions: on standard GC agar (GC base (Oxoid) with Kellogg’s supplements [ ]); on GC agar with sub-lethal monocaprin (Sigma-Aldrich; C10:0MG; 125 μM); and on GC agar with sub-lethal myristic acid (Sigma-Aldrich; C14:0; 62.5 μM). .. Minimally passaged N . gonorrhoeae strain NCCP11945 received from the sequencing project [ ] was incubated at 37°C, 5% CO2 for 48 hours.

Article Title: Typing of Neisseria gonorrhoeae Reveals Rapid Reinfection in Rural South Africa
Article Snippet: In brief, twofold serial dilutions of antibiotics were added to molten GC agar base (Oxoid Ltd.) plus 1% Iso VitaleX supplement at 45°C. .. The plates were incubated at 37°C under CO2 for 24 h. N. gonorrhoeae ATCC 49226, Staphylococcus aureus ATCC 25923, and Escherichia coli ATCC 10418 were used as controls.

Diffusion-based Assay:

Article Title: Emergence of Fluoroquinolone Resistance in Neisseria gonorrhoeae Isolates from Four Clinics in Three Regions of Kenya
Article Snippet: .. Susceptibility testing to cefixime, ceftriaxone, azithromycin, ciprofloxacin and/or norfloxacin was performed using antibiotic disk diffusion or epilometry (E-test, BioMerieux, Marseille, France) on GC base agar (Oxoid, Basingstoke, UK) with 1% IsoVitalex (Oxoid) supplement. ..

Article Title: Antibiotic Susceptibility Monitoring of Neisseria gonorrhoeae in Bacolod City, Philippines
Article Snippet: Antibiotic Susceptibility Testing Testing for the isolates’ susceptibility was carried out by Kirby-Bauer disc diffusion assay [ , ] in accordance with the guidelines and criteria of the Clinical Laboratory Standards Institute [ ] and the Centers for Disease Control and Prevention [ ]. .. The entire surface of the GC agar (Oxoid) supplemented with 1% vitox (Oxoid) was then inoculated in three different directions to ensure uniform and confluent growth.

Activity Assay:

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: Gram-negative diplococci showing oxidase positivity, gamma-glutamyltransferase activity, metabolism of glucose and maltose in a cystine-Trypticase agar medium, and a negative ONPG ( o -nitrophenyl-β- d -galactopyranoside) reaction were identified as N. meningitidis . .. In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid).

Expressing:

Article Title: Pilus Phase Variation Switches Gonococcal Adherence to Invasion by Caveolin-1-Dependent Host Cell Signaling
Article Snippet: N2013 was generated by transforming the PorBIA expression cassette from strain N927 into strain N503 (inducible rec A, erm C). .. Gonococci were routinely grown on GC agar base plates (Oxoid) supplemented with 1% vitamin mix for 14–18 h at 37°C in 5% CO2 in a humidified atmosphere.

Modification:

Article Title: Emergence of Fluoroquinolone Resistance in Neisseria gonorrhoeae Isolates from Four Clinics in Three Regions of Kenya
Article Snippet: When clinically indicated, patients had urethral or cervical swabs collected that were cultured directly to modified Thayer-Martin medium. .. Susceptibility testing to cefixime, ceftriaxone, azithromycin, ciprofloxacin and/or norfloxacin was performed using antibiotic disk diffusion or epilometry (E-test, BioMerieux, Marseille, France) on GC base agar (Oxoid, Basingstoke, UK) with 1% IsoVitalex (Oxoid) supplement.

Article Title: Molecular Investigation of Tularemia Outbreaks, Spain, 1997-2008
Article Snippet: .. All isolates were grown on modified Thayer-Martin agar plates containing 36 g/L GC agar base, 10 g/L soluble hemoglobin powder, and 2 vials/L Vitox supplement (Oxoid, Basingstoke, UK) at 37°C for 2–3 days in aerobic conditions. ..

Article Title: Methods for Identifying Neisseria meningitidis Carriers: A Multi-Center Study in the African Meningitis Belt
Article Snippet: .. Modified Thayer-Martin (TM) selective agar plates were prepare locally using Gonococci agar base (Oxoid CM0367B), hemoglobin powder (Oxoid LP053B) containing 3mg/liter vancomycin, 7.5mg/liter colistin, 12.5 U/liter nystatin, 5mg/liter trimetropin lactate (Oxoid SR00991E) and Vitox enrichment supplement (Oxoid SR0090A) (Thermo Scientific, UK) [ ]. ..

Western Blot:

Article Title: Monitoring Antimicrobial Susceptibility of Neisseria gonorrhoeae Isolated from Bangladesh during 1997-2006: Emergence and Pattern of Drug-resistant Isolates
Article Snippet: Ten microlitre of the bacterial suspension (1×104 cfu) was spotted on GC agar (Oxoid Ltd, Basingstoke, Hampshaire, UK) plates as recommended by the WHO, containing two-fold serial dilution of antimicrobial agents using a multi-point inoculator (Mast Diagnostic Ltd., SCAN 114). .. The quality control of the MIC test was ensured by including seven N. gonorrhoeae reference strains WHO A, B, C, D, E, G, and H with known MICs in each test, by repeating each test three times and by participating in the WPRO/SEARO GASP (Western Pacific Region Office/South-East Asia Regional Office/Gonococcal Antimicrobial Surveillance Programme) external quality-assurance programme (our data were in agreement with expected results for all antimicrobial agents during the study period).

Agglutination:

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid). .. The Gram-negative diplococci and oxidase-positive meningococci colonies were serogrouped by slide agglutination tests with serogroup-specific antisera (A, B, C, X, Y, Z, W-135, and 29E; Difco/BD, Franklin Lakes, NJ).

Article Title: Differences in the population structure of Neisseria meningitidis in two Australian states: Victoria and Western Australia
Article Snippet: Isolates were passaged fewer than 5 times and were cultured under aerobic conditions with 5% CO2 at 37°C on GC agar (Oxoid) supplemented with 0.4% glucose, 0.01% glutamine, 0.2 mg/L of cocarboxylase and 5 mg/L of iron(III) nitrate. .. Serogroup-specific antisera were used to determine the serogroup of each isolate using slide agglutination.

Inhibition:

Article Title: Antibiotic Susceptibility Monitoring of Neisseria gonorrhoeae in Bacolod City, Philippines
Article Snippet: The entire surface of the GC agar (Oxoid) supplemented with 1% vitox (Oxoid) was then inoculated in three different directions to ensure uniform and confluent growth. .. Azithromycin was not included for antibiotic testing because the breakpoints for the interpretation of its zone of inhibition using the Clinical and Laboratory Standards Institute (CLSI) [ ] guidelines are not available.

Serial Dilution:

Article Title: Monitoring Antimicrobial Susceptibility of Neisseria gonorrhoeae Isolated from Bangladesh during 1997-2006: Emergence and Pattern of Drug-resistant Isolates
Article Snippet: .. Ten microlitre of the bacterial suspension (1×104 cfu) was spotted on GC agar (Oxoid Ltd, Basingstoke, Hampshaire, UK) plates as recommended by the WHO, containing two-fold serial dilution of antimicrobial agents using a multi-point inoculator (Mast Diagnostic Ltd., SCAN 114). .. The quality control of the MIC test was ensured by including seven N. gonorrhoeae reference strains WHO A, B, C, D, E, G, and H with known MICs in each test, by repeating each test three times and by participating in the WPRO/SEARO GASP (Western Pacific Region Office/South-East Asia Regional Office/Gonococcal Antimicrobial Surveillance Programme) external quality-assurance programme (our data were in agreement with expected results for all antimicrobial agents during the study period).

Cell Culture:

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: .. In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid). .. All suspected meningococcal colonies were characterized by Gram stain and oxidase tests.

Article Title: Emergence of Fluoroquinolone Resistance in Neisseria gonorrhoeae Isolates from Four Clinics in Three Regions of Kenya
Article Snippet: When clinically indicated, patients had urethral or cervical swabs collected that were cultured directly to modified Thayer-Martin medium. .. Susceptibility testing to cefixime, ceftriaxone, azithromycin, ciprofloxacin and/or norfloxacin was performed using antibiotic disk diffusion or epilometry (E-test, BioMerieux, Marseille, France) on GC base agar (Oxoid, Basingstoke, UK) with 1% IsoVitalex (Oxoid) supplement.

Article Title: Differences in the population structure of Neisseria meningitidis in two Australian states: Victoria and Western Australia
Article Snippet: .. Isolates were passaged fewer than 5 times and were cultured under aerobic conditions with 5% CO2 at 37°C on GC agar (Oxoid) supplemented with 0.4% glucose, 0.01% glutamine, 0.2 mg/L of cocarboxylase and 5 mg/L of iron(III) nitrate. .. Serogroup-specific antisera were used to determine the serogroup of each isolate using slide agglutination.

Generated:

Article Title: Pilus Phase Variation Switches Gonococcal Adherence to Invasion by Caveolin-1-Dependent Host Cell Signaling
Article Snippet: N2013 was generated by transforming the PorBIA expression cassette from strain N927 into strain N503 (inducible rec A, erm C). .. Gonococci were routinely grown on GC agar base plates (Oxoid) supplemented with 1% vitamin mix for 14–18 h at 37°C in 5% CO2 in a humidified atmosphere.

other:

Article Title: The structure of the L9 immunotype lipooligosaccharide from Neisseria meningitidis NMA Z2491
Article Snippet: Meningococcal strain NMA Z2491 was grown on GC agar base (Oxoid) supplemented with 20 mM glucose, 0.43 µM thiamine pyrophosphate chloride, 6.8 mM glutamine and 12.4 µM Fe(NO3 )3 in a 5% carbon dioxide atmosphere.

Polymerase Chain Reaction:

Article Title: Microbiological Analysis from a Phase 2 Randomized Study in Adults Evaluating Single Oral Doses of Gepotidacin in the Treatment of Uncomplicated Urogenital Gonorrhea Caused by Neisseria gonorrhoeae
Article Snippet: The PCR primers for the amplification of N. gonorrhoeae gyrA and parC ). .. To assess FoR, 2 separate studies were performed by GlaxoSmithKline (Collegeville, PA) using Oxoid GC agar base containing BB BBL IsovitaleX enrichment in study 1 and Remel agar base plus hemoglobin and BB BBL IsovitaleX Enrichment in study 2.

Isolation:

Article Title: DNA Methylation Assessed by SMRT Sequencing Is Linked to Mutations in Neisseria meningitidis Isolates
Article Snippet: Paragraph title: Cultivation of strains of N. meningitidis, isolation of genomic DNA ... For genomic DNA preparation, strains were grown on supplemented GC agar base (Oxoid) plates for 20–24 hours in 5% CO2 at 37°C.

Article Title: Interstrain Cooperation in Meningococcal Biofilms: Role of Autotransporters NalP and AutA
Article Snippet: Neisserial strains were grown overnight on GC agar base medium (OXOID) supplemented with Oxoid™ Vitox at 37°C in a CO2 -enriched atmosphere provided by a candle jar. .. BB-1 and HB-1 are capsule-deficient derivatives of strains B16B6 (Frasch and Chapman, ) and H44/76 (Holten, ), respectively, which were isolated from patients with meningococcal disease in the United States and Norway, respectively.

Article Title: Molecular Investigation of Tularemia Outbreaks, Spain, 1997-2008
Article Snippet: Source of isolates, subspecies, host, geographic origin, and year of isolation are shown in the . .. All isolates were grown on modified Thayer-Martin agar plates containing 36 g/L GC agar base, 10 g/L soluble hemoglobin powder, and 2 vials/L Vitox supplement (Oxoid, Basingstoke, UK) at 37°C for 2–3 days in aerobic conditions.

Article Title: Differences in the population structure of Neisseria meningitidis in two Australian states: Victoria and Western Australia
Article Snippet: Bacterial strains and growth conditions A total of 201 meningococcal strains isolated from disease cases were used in this study– 131 from VIC and 70 from WA ( ). .. Isolates were passaged fewer than 5 times and were cultured under aerobic conditions with 5% CO2 at 37°C on GC agar (Oxoid) supplemented with 0.4% glucose, 0.01% glutamine, 0.2 mg/L of cocarboxylase and 5 mg/L of iron(III) nitrate.

Microscopy:

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: Identification of Neisseria meningitidis was performed by Gram stain microscopy and biochemical analysis. .. In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid).

Article Title: Pilus Phase Variation Switches Gonococcal Adherence to Invasion by Caveolin-1-Dependent Host Cell Signaling
Article Snippet: Gonococci were routinely grown on GC agar base plates (Oxoid) supplemented with 1% vitamin mix for 14–18 h at 37°C in 5% CO2 in a humidified atmosphere. .. Opa and pili negative phenotypes were monitored by colony morphology under a stereo microscope or by immunoblotting.

Sequencing:

Article Title: Mutations in Neisseria gonorrhoeae grown in sub-lethal concentrations of monocaprin do not confer resistance
Article Snippet: N . gonorrhoeae strain NCCP11945 was grown in three conditions: on standard GC agar (GC base (Oxoid) with Kellogg’s supplements [ ]); on GC agar with sub-lethal monocaprin (Sigma-Aldrich; C10:0MG; 125 μM); and on GC agar with sub-lethal myristic acid (Sigma-Aldrich; C14:0; 62.5 μM). .. Minimally passaged N . gonorrhoeae strain NCCP11945 received from the sequencing project [ ] was incubated at 37°C, 5% CO2 for 48 hours.

Article Title: DNA Methylation Assessed by SMRT Sequencing Is Linked to Mutations in Neisseria meningitidis Isolates
Article Snippet: N . meningitidis isolates were previously collected over a time period of ~10 years during meningococcal meningitis epidemics in Sub-Saharan Africa (two sequence types ST2859 and ST7). .. For genomic DNA preparation, strains were grown on supplemented GC agar base (Oxoid) plates for 20–24 hours in 5% CO2 at 37°C.

Article Title: Mutations in Neisseria gonorrhoeae grown in sub-lethal concentrations of monocaprin do not confer resistance
Article Snippet: Bacterial growth and experimental evolution N . gonorrhoeae strain NCCP11945 was grown in three conditions: on standard GC agar (GC base (Oxoid) with Kellogg’s supplements [ ]); on GC agar with sub-lethal monocaprin (Sigma-Aldrich; C10:0MG; 125 μM); and on GC agar with sub-lethal myristic acid (Sigma-Aldrich; C14:0; 62.5 μM). .. Minimally passaged N . gonorrhoeae strain NCCP11945 received from the sequencing project [ ] was incubated at 37°C, 5% CO2 for 48 hours.

Software:

Article Title: Microbiological Analysis from a Phase 2 Randomized Study in Adults Evaluating Single Oral Doses of Gepotidacin in the Treatment of Uncomplicated Urogenital Gonorrhea Caused by Neisseria gonorrhoeae
Article Snippet: Lasergene SeqMan software (DYNASTAR, Inc., Madison, WI) was used to identify nucleotide changes resulting in amino acid residue substitutions.). .. To assess FoR, 2 separate studies were performed by GlaxoSmithKline (Collegeville, PA) using Oxoid GC agar base containing BB BBL IsovitaleX enrichment in study 1 and Remel agar base plus hemoglobin and BB BBL IsovitaleX Enrichment in study 2.

In Vitro:

Article Title: DNA Methylation Assessed by SMRT Sequencing Is Linked to Mutations in Neisseria meningitidis Isolates
Article Snippet: Isolates underwent typically 2 rounds of single colony sub-culturing and over-night expansion in vitro . .. For genomic DNA preparation, strains were grown on supplemented GC agar base (Oxoid) plates for 20–24 hours in 5% CO2 at 37°C.

Staining:

Article Title: Acquisition of Meningococcal Serogroup W-135 Carriage in Turkish Hajj Pilgrims Who Had Received the Quadrivalent Meningococcal Polysaccharide Vaccine
Article Snippet: Identification of Neisseria meningitidis was performed by Gram stain microscopy and biochemical analysis. .. In the evaluation of household contacts conducted 2 to 3 months after identification of carriage in a pilgrim, samples were cultured on GC agar base (Oxoid, Cambridge, United Kingdom) containing 5% hemoglobin (Oxoid), yeast autolysate, Vitox growth supplement (Oxoid), and VCNT antibiotic supplements (vancomycin, colistin methane sulfonate, nystatin, and trimethoprim; Oxoid).

Variant Assay:

Article Title: Pilus Phase Variation Switches Gonococcal Adherence to Invasion by Caveolin-1-Dependent Host Cell Signaling
Article Snippet: N2009 (PorBIA , Opa− , P+ ) is a piliated variant of N138 expressing PorBIA from N927. .. Gonococci were routinely grown on GC agar base plates (Oxoid) supplemented with 1% vitamin mix for 14–18 h at 37°C in 5% CO2 in a humidified atmosphere.

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    Thermo Fisher gc agar base
    Gc Agar Base, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc agar base/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gc agar base - by Bioz Stars, 2020-03
    95/100 stars
      Buy from Supplier

    99
    Thermo Fisher gc agar plates
    Gc Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc agar plates/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    gc agar plates - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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