gateway vector conversion kit  (Thermo Fisher)


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    Name:
    Gateway Vector Conversion System
    Description:
    The Gateway Vector Conversion System is designed to convert any vector into a Gateway destination vector using restriction endonucleases and ligase A Gateway cassette containing attR recombination sites flanking a ccdB gene 1 and a chloramphenicol resistance gene are blunt end cloned into the multiple cloning site of any vector ccdB Survival 2 competent cells allow propagation of the Gateway vectors containing the ccdB gene The Gateway Vector Conversion System offers • Conversion of any expression vector into a Gateway destination vector• Three cassettes to construct a destination vector in the correct reading frame Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation
    Catalog Number:
    11828029
    Price:
    None
    Applications:
    Cloning|Gateway Vector Conversion Systems|Gateway Cloning
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher gateway vector conversion kit
    The Gateway Vector Conversion System is designed to convert any vector into a Gateway destination vector using restriction endonucleases and ligase A Gateway cassette containing attR recombination sites flanking a ccdB gene 1 and a chloramphenicol resistance gene are blunt end cloned into the multiple cloning site of any vector ccdB Survival 2 competent cells allow propagation of the Gateway vectors containing the ccdB gene The Gateway Vector Conversion System offers • Conversion of any expression vector into a Gateway destination vector• Three cassettes to construct a destination vector in the correct reading frame Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation
    https://www.bioz.com/result/gateway vector conversion kit/product/Thermo Fisher
    Average 98 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    gateway vector conversion kit - by Bioz Stars, 2020-08
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: NOTCH1 signaling in oral squamous cell carcinoma via a TEL2/SERPINE1 axis
    Article Snippet: .. Plasmid constructs and transfection To achieve constitutive expression of NICD, 3xFlagNICD1 (Addgene #20183, [ ]) was cloned into the multiple cloning site, under the SFFV promoter, of the LeGO-iC2 plasmid (Addgene#27345, [ ]) using the Gateway Vector Conversion System (ThermoFisher Scientific). ..

    Article Title: A novel germline variant in CSF3R reduces N-glycosylation and exerts potent oncogenic effects in leukemia
    Article Snippet: .. MSCV-IRES-GFP (MigR1) was made compatible for Gateway cloning using the Gateway Vector Conversion Kit (Invitrogen). .. A gateway pDONR vector for CSF3R transcript variant 1 (NM_00760.2) was purchased (GeneCopoeia).

    Article Title: Hsp72 (HSPA1A) Prevents Human Islet Amyloid Polypeptide Aggregation and Toxicity: A New Approach for Type 2 Diabetes Treatment
    Article Snippet: .. The resulting constructs were digested with Sal I, blunt-ended and then ligated with the Gateway Vector Conversion Reading Frame Cassette C.1 (Life Technologies, Grand Island, NY) to generate Gateway destination vectors pNG1 and pNG2 that contained the human and mouse preproIAPP clones, respectively. .. Entry clones pLR22, pLR25 and pLR35 contained the C . elegans promoter regions of lev-11 (body-wall muscles), tnt-4 (pharynx) and aex-3 (pan-neuronal), respectively.

    Article Title: A Gateway MultiSite Recombination Cloning Toolkit
    Article Snippet: .. Third, the 1.7 kb Gateway cassette RF C.1 (Invitrogen Cat # 11828-029) was cloned into the Hpa I site of the resulting vector from step two. .. The resulting vector from step three we designate pDESThawP.

    Article Title: Molecular cloning, characterization, genomic organization and promoter analysis of the ?1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0
    Article Snippet: .. Human coding sequence of PRDM1, IRF3 and MZF1 were cloned into the pcDNA3.1 expression vector using the Gateway® conversion system (Invitrogen, Carlsbad, CA, USA). .. The pcDNA3.1 vector containing the human KLF15 sequence was kindly provided by Dr. Otteson (College of Optometry, University of Houston, TX, USA) and the pcDNA3.1 vector containing the human sequence of PAX5 was provided by Dr. Broccardo (Centre de Physiopathologie INSERM U563, Toulouse, France).

    Transfection:

    Article Title: NOTCH1 signaling in oral squamous cell carcinoma via a TEL2/SERPINE1 axis
    Article Snippet: .. Plasmid constructs and transfection To achieve constitutive expression of NICD, 3xFlagNICD1 (Addgene #20183, [ ]) was cloned into the multiple cloning site, under the SFFV promoter, of the LeGO-iC2 plasmid (Addgene#27345, [ ]) using the Gateway Vector Conversion System (ThermoFisher Scientific). ..

    Construct:

    Article Title: NOTCH1 signaling in oral squamous cell carcinoma via a TEL2/SERPINE1 axis
    Article Snippet: .. Plasmid constructs and transfection To achieve constitutive expression of NICD, 3xFlagNICD1 (Addgene #20183, [ ]) was cloned into the multiple cloning site, under the SFFV promoter, of the LeGO-iC2 plasmid (Addgene#27345, [ ]) using the Gateway Vector Conversion System (ThermoFisher Scientific). ..

    Article Title: Hsp72 (HSPA1A) Prevents Human Islet Amyloid Polypeptide Aggregation and Toxicity: A New Approach for Type 2 Diabetes Treatment
    Article Snippet: .. The resulting constructs were digested with Sal I, blunt-ended and then ligated with the Gateway Vector Conversion Reading Frame Cassette C.1 (Life Technologies, Grand Island, NY) to generate Gateway destination vectors pNG1 and pNG2 that contained the human and mouse preproIAPP clones, respectively. .. Entry clones pLR22, pLR25 and pLR35 contained the C . elegans promoter regions of lev-11 (body-wall muscles), tnt-4 (pharynx) and aex-3 (pan-neuronal), respectively.

    Modification:

    Article Title: Development of Antigen-Specific Memory CD8+ T Cells Following Live-Attenuated Chimeric West Nile Virus Vaccination
    Article Snippet: .. The vaccinia transfer plasmid pMJ601 was modified to facilitate insertion of the WNV E gene (Gateway Vector conversion system; Invitrogen). .. Recombinant vaccinia viruses were generated by homologous recombination and propagated in CV-1 cells [ ].

    Expressing:

    Article Title: NOTCH1 signaling in oral squamous cell carcinoma via a TEL2/SERPINE1 axis
    Article Snippet: .. Plasmid constructs and transfection To achieve constitutive expression of NICD, 3xFlagNICD1 (Addgene #20183, [ ]) was cloned into the multiple cloning site, under the SFFV promoter, of the LeGO-iC2 plasmid (Addgene#27345, [ ]) using the Gateway Vector Conversion System (ThermoFisher Scientific). ..

    Article Title: Molecular cloning, characterization, genomic organization and promoter analysis of the ?1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0
    Article Snippet: .. Human coding sequence of PRDM1, IRF3 and MZF1 were cloned into the pcDNA3.1 expression vector using the Gateway® conversion system (Invitrogen, Carlsbad, CA, USA). .. The pcDNA3.1 vector containing the human KLF15 sequence was kindly provided by Dr. Otteson (College of Optometry, University of Houston, TX, USA) and the pcDNA3.1 vector containing the human sequence of PAX5 was provided by Dr. Broccardo (Centre de Physiopathologie INSERM U563, Toulouse, France).

    Sequencing:

    Article Title: Molecular cloning, characterization, genomic organization and promoter analysis of the ?1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0
    Article Snippet: .. Human coding sequence of PRDM1, IRF3 and MZF1 were cloned into the pcDNA3.1 expression vector using the Gateway® conversion system (Invitrogen, Carlsbad, CA, USA). .. The pcDNA3.1 vector containing the human KLF15 sequence was kindly provided by Dr. Otteson (College of Optometry, University of Houston, TX, USA) and the pcDNA3.1 vector containing the human sequence of PAX5 was provided by Dr. Broccardo (Centre de Physiopathologie INSERM U563, Toulouse, France).

    Plasmid Preparation:

    Article Title: Development of Antigen-Specific Memory CD8+ T Cells Following Live-Attenuated Chimeric West Nile Virus Vaccination
    Article Snippet: .. The vaccinia transfer plasmid pMJ601 was modified to facilitate insertion of the WNV E gene (Gateway Vector conversion system; Invitrogen). .. Recombinant vaccinia viruses were generated by homologous recombination and propagated in CV-1 cells [ ].

    Article Title: NOTCH1 signaling in oral squamous cell carcinoma via a TEL2/SERPINE1 axis
    Article Snippet: .. Plasmid constructs and transfection To achieve constitutive expression of NICD, 3xFlagNICD1 (Addgene #20183, [ ]) was cloned into the multiple cloning site, under the SFFV promoter, of the LeGO-iC2 plasmid (Addgene#27345, [ ]) using the Gateway Vector Conversion System (ThermoFisher Scientific). ..

    Article Title: A novel germline variant in CSF3R reduces N-glycosylation and exerts potent oncogenic effects in leukemia
    Article Snippet: .. MSCV-IRES-GFP (MigR1) was made compatible for Gateway cloning using the Gateway Vector Conversion Kit (Invitrogen). .. A gateway pDONR vector for CSF3R transcript variant 1 (NM_00760.2) was purchased (GeneCopoeia).

    Article Title: Hsp72 (HSPA1A) Prevents Human Islet Amyloid Polypeptide Aggregation and Toxicity: A New Approach for Type 2 Diabetes Treatment
    Article Snippet: .. The resulting constructs were digested with Sal I, blunt-ended and then ligated with the Gateway Vector Conversion Reading Frame Cassette C.1 (Life Technologies, Grand Island, NY) to generate Gateway destination vectors pNG1 and pNG2 that contained the human and mouse preproIAPP clones, respectively. .. Entry clones pLR22, pLR25 and pLR35 contained the C . elegans promoter regions of lev-11 (body-wall muscles), tnt-4 (pharynx) and aex-3 (pan-neuronal), respectively.

    Article Title: A Gateway MultiSite Recombination Cloning Toolkit
    Article Snippet: .. Third, the 1.7 kb Gateway cassette RF C.1 (Invitrogen Cat # 11828-029) was cloned into the Hpa I site of the resulting vector from step two. .. The resulting vector from step three we designate pDESThawP.

    Article Title: Molecular cloning, characterization, genomic organization and promoter analysis of the ?1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0
    Article Snippet: .. Human coding sequence of PRDM1, IRF3 and MZF1 were cloned into the pcDNA3.1 expression vector using the Gateway® conversion system (Invitrogen, Carlsbad, CA, USA). .. The pcDNA3.1 vector containing the human KLF15 sequence was kindly provided by Dr. Otteson (College of Optometry, University of Houston, TX, USA) and the pcDNA3.1 vector containing the human sequence of PAX5 was provided by Dr. Broccardo (Centre de Physiopathologie INSERM U563, Toulouse, France).

    Article Title: Construction of three new Gateway® expression plasmids forTrypanosoma cruzi
    Article Snippet: .. The 5’-phosphates were removed with calf intestinal alkaline phosphatase and the plasmid was ligated with the Gateway® cassette Rf A (Gateway® Vector Conversion System, Invitrogen) to generate the destination vector , which was maintained in the ccdB Survival™ 2 T1R E. coli strain. .. To determine the functionality of the obtained pTc INDEX-GW vector, the GFP open reading frame was transferred by recombination (LR) with LR clonase II enzyme (Invitrogen) from the entry vector pENTR3C-GFP as previously described.

    Article Title: Dynamic Modification of Sphingomyelin in Lipid Microdomains Controls Development of Obesity, Fatty Liver, and Type 2 Diabetes *
    Article Snippet: .. The pQCXIP (Clontech), pQCXIH (Clontech), pHcRed (Clontech), and p3×FLAG-myc-CMV26 (Sigma) vectors were converted to destination vectors using the Gateway® vector conversion system (Invitrogen), producing pQCXIP-DEST, pQCXIH-DEST, pHcRed-DEST, and p3×FLAG-DEST, respectively. .. Gateway®LR reactions were performed as described in the Gateway® instruction manual (Invitrogen), to finally produce the pcDNA6.2-SMS1-V5, pcDNA6.2-SMS2-V5, pcDNA6.2-CAV1, pcDNA6.2-SMS2-EmGFP, pHcRed-CD36/FAT, pHcRed-caveolin 1, p3×FLAG-CD36/FAT, p3×FLAG-caveolin 1, pQCXIP-SMS1-V5, and pQCXIH-SMS2-V5 plasmids.

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  • 98
    Thermo Fisher gateway vector conversion kit
    Gateway Vector Conversion Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway vector conversion kit/product/Thermo Fisher
    Average 98 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    gateway vector conversion kit - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

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