gat-3  (Alomone Labs)


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    Alomone Labs gat-3
    Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gat-3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gat-3 - by Bioz Stars, 2023-02
    93/100 stars

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    gat-3  (Alomone Labs)


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    Alomone Labs gat-3
    Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gat-3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gat-3 - by Bioz Stars, 2023-02
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    anti gat 3  (Alomone Labs)


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    Alomone Labs anti gat 3
    The expressions of neurotransmitters and different subunits of GABA A Rs. A‐B, The expression of GABA was decreased in the laparotomy mice, and no difference was found about glutamate (n = 9). C, The different average spectra of selected metabolites (GABA and glutamate). D‐F, The mRNA level of α5 subunit was up‐regulated at 1 day and continued to 10 days after laparotomy. No difference was found about the α1 and β3 subunits (n = 3). G‐I, The expressions of <t>GAT‐3</t> and GAD65 were decreased, and the levels of surface α5GABA A Rs were increased in the laparotomy mice (n = 4). Data are presented as mean ± SEM. * P < .05, ** P < .01, ***P<0.001, ****P<0.0001
    Anti Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gat 3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gat 3 - by Bioz Stars, 2023-02
    93/100 stars

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    1) Product Images from "Disruption of the GABAergic system contributes to the development of perioperative neurocognitive disorders after anesthesia and surgery in aged mice"

    Article Title: Disruption of the GABAergic system contributes to the development of perioperative neurocognitive disorders after anesthesia and surgery in aged mice

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.13388

    The expressions of neurotransmitters and different subunits of GABA A Rs. A‐B, The expression of GABA was decreased in the laparotomy mice, and no difference was found about glutamate (n = 9). C, The different average spectra of selected metabolites (GABA and glutamate). D‐F, The mRNA level of α5 subunit was up‐regulated at 1 day and continued to 10 days after laparotomy. No difference was found about the α1 and β3 subunits (n = 3). G‐I, The expressions of GAT‐3 and GAD65 were decreased, and the levels of surface α5GABA A Rs were increased in the laparotomy mice (n = 4). Data are presented as mean ± SEM. * P < .05, ** P < .01, ***P<0.001, ****P<0.0001
    Figure Legend Snippet: The expressions of neurotransmitters and different subunits of GABA A Rs. A‐B, The expression of GABA was decreased in the laparotomy mice, and no difference was found about glutamate (n = 9). C, The different average spectra of selected metabolites (GABA and glutamate). D‐F, The mRNA level of α5 subunit was up‐regulated at 1 day and continued to 10 days after laparotomy. No difference was found about the α1 and β3 subunits (n = 3). G‐I, The expressions of GAT‐3 and GAD65 were decreased, and the levels of surface α5GABA A Rs were increased in the laparotomy mice (n = 4). Data are presented as mean ± SEM. * P < .05, ** P < .01, ***P<0.001, ****P<0.0001

    Techniques Used: Expressing

    rabbit anti gat 3  (Alomone Labs)


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    Alomone Labs rabbit anti gat 3
    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by <t>GAT-3</t> inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Rabbit Anti Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gat 3/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gat 3 - by Bioz Stars, 2023-02
    96/100 stars

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    1) Product Images from "Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior"

    Article Title: Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior

    Journal: Neuron

    doi: 10.1016/j.neuron.2018.08.015

    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Figure Legend Snippet: (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.

    Techniques Used: Expressing, Fluorescence, MANN-WHITNEY, Immunostaining, Western Blot, Inhibition

    (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.
    Figure Legend Snippet: (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.

    Techniques Used: RNA Sequencing Assay, Expressing, In Vivo

    rabbit anti gat 3  (Alomone Labs)


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    Alomone Labs rabbit anti gat 3
    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by <t>GAT-3</t> inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Rabbit Anti Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gat 3/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gat 3 - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior"

    Article Title: Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior

    Journal: Neuron

    doi: 10.1016/j.neuron.2018.08.015

    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Figure Legend Snippet: (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.

    Techniques Used: Expressing, Fluorescence, MANN-WHITNEY, Immunostaining, Western Blot, Inhibition

    (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.
    Figure Legend Snippet: (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.

    Techniques Used: RNA Sequencing Assay, Expressing, In Vivo

    rabbit anti gat 3  (Alomone Labs)


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    Alomone Labs rabbit anti gat 3
    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by <t>GAT-3</t> inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Rabbit Anti Gat 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gat 3/product/Alomone Labs
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    1) Product Images from "Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior"

    Article Title: Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior

    Journal: Neuron

    doi: 10.1016/j.neuron.2018.08.015

    (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.
    Figure Legend Snippet: (A) Current recordings in voltage-clamp (−60 mV) from MSNs in control and hPMCA2w/b-expressing mice. Dashed lines and arrows indicate the changes of baseline induced by bicuculline application (BIC; 25 μM). Histograms show the shift in baseline currents recorded during BIC application. (B) Tonic GABA currents were reduced in MSNs from hPMCA2w/b-expressing mice, and this effect was rescued by GAT-3 inhibitor SNAP5114 (40 μM). (C) Left, images of GAT-3 IHC in astrocytes expressing control protein or hPMCA2w/b. Right, quantification of GAT-3 fluorescence intensity in hPMCA2w/b-expressing astrocytes relative to those expressing tdTomato (Mann-Whitney test). (D-E) As in C, but for Kir4.1 and S100β immunostaining. (F) Western blot analysis of striatum membrane protein fractions showing for GAT-3 and GAT-1 levels in hPMCA2w/b groups relative to controls. The gel shown has been cropped to fit in the figure and the crop lines are shown as a solid border. The data were normalized to tubulin as a loading control. (G, H) Tonic GABA inhibition was decreased in D1-MSNs (G), but not D2-MSNs (H) in hPMCA2w/b-expressing mice (assessed with Student’s t or Mann-Whitney tests). The MSNs are colored, but were from Drd1-cre and Adora2a-cre mice with FLEX AAV tdTomato injections. (I) Behavioral traces of control and hPMCA2w/b-expressing mice under baseline and SNAP5114 treatment showing self-grooming and non-grooming episodes over 10 min. (J) Increased self-grooming behaviors in hPMCA2w/b-expressing mice were rescued by SNAP5114 (50 μmol/kg; data were assessed with One-way ANOVA followed by post hoc Bonferroni tests). (K) AAV2/5 overexpressing GAT-3 in astrocytes and GAT-3HA detection in S100β positive astrocytes. (L) Traces for self-grooming. (M) Average data for self-grooming duration and bouts for controls and GAT-3HA overexpressing mice. Data were assessed with Student’s t test. The average data are shown as mean ± SEM.

    Techniques Used: Expressing, Fluorescence, MANN-WHITNEY, Immunostaining, Western Blot, Inhibition

    (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.
    Figure Legend Snippet: (A) Hierarchical cluster dendrogram with colors underneath denoting three distinct modules of co-expressed transcripts in RiboTag mice RNA-seq data. (B) Heat map showing module-trait relationships, each row representing a module. Correlation coefficients between a Module Eigengene (ME) and trait (coded from −1 to 1) and corresponding P values are shown in each cell. The color indicates the level of correlation (positive correlation in red and negative correlation in green). Individual plots showing the expression of the module Eigengene across sample categories with significantly enriched terms for biological processes revealed by gene ontology enrichment analysis (FDR < 0.05). (C) Cluster dendrogram identified 16 co-expressed WGCNA modules for RiboTag AAV RNA-seq data. (D) Module-trait relationships revealed modules correlated with hPMCA2w/b expression in striatal astrocytes. Green module was composed of genes regulating protein transport, which were down-regulated in striatal astrocytes expressing hPMCA2w/b. Pink module was enriched in genes regulating chromatin silencing and transcription, which were upregulated in striatal astrocytes expressing hPMCA2w/b. (E) Expression levels of GABA transporters: Slc6a1 (GAT-1), Slc6a13 (GAT-2), Slc6a11 (GAT-3) and Slc6a12 (GAT-4) in RiboTag mice and RiboTag AAV RNA-seq data sets. (F) Expression levels of potential GABA transporter regulators suggested by previous work in RiboTag mice and RiboTag AAV RNA-seq data. (G) Changes in gene expression of striatal astrocytes expressing hPMCA2w/b, expressed as log2 (fold-change), in RiboTag mice and RiboTag AAV RNA-seq data. Asterisk (*) indicates differential expression between hPMCA2w/b IP and control IP using edgeR analysis on combined RNA-seq datasets (FDR < 0.1). (H-I) Cartoon summary of the main findings at synaptic (H) and in vivo levels (I). (J) Descriptive model based on our work (see discussion). Average data shown as mean ± SEM from 4 mice in each group.

    Techniques Used: RNA Sequencing Assay, Expressing, In Vivo

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