salmonella enterica serovar gallinarum  (ATCC)

Journal: Vaccines

Article Title: The Evolution of Vaccines Development across Salmonella Serovars among Animal Hosts: A Systematic Review

doi: 10.3390/vaccines12091067

Figure Lengend Snippet: A summary of commercial vaccines against salmonellosis in different animal models.

Article Snippet: NOBILIS 9R Vac ® , CEVA Animal Health, Lenexa, KS, USA , Chicken , Live-attenuated S . Gallinarum.

Techniques: Vaccines, Mutagenesis

Journal: Animals : an Open Access Journal from MDPI

Article Title: Genetic Characterization of the Co-Invasive Rodent Parasite Heterakis spumosa (Nematoda, Heterakidae)

doi: 10.3390/ani14182674

Figure Lengend Snippet: Maximum likelihood (Kimura 2 parameter model) phylogeny using the nuclear ITS1 region as the genetic marker. The numbers at the nodes indicate bootstrap support obtained through 1000 replications (ML/NJ). Only bootstrap values >50 are shown. Reference NCBI sequences are shown with their accession numbers. The representative sequences for H. spumosa and H. gallinarum are highlighted in ‘red’ and ‘blue’, respectively, with the host, location, and host ID given in parentheses.

Article Snippet: As an outgroup of the genus, we collected Heterakis from Gallus gallus (Linnaeus, 1758) collected in Udon Thani Province (Thailand), as well as H. gallinarum (GenBank accession number KP308348, KT310157, LC592777); H. beramporia Lane, 1914 (GenBank accession number: KU529971); H. dispar (Schrank, 1790) (GenBank accession number MF319969, NC042411, OM530143); H. isolonche Linstow, 1906 (GenBank accession number KM212953).

Techniques: Marker

Journal: Animals : an Open Access Journal from MDPI

Article Title: Genetic Characterization of the Co-Invasive Rodent Parasite Heterakis spumosa (Nematoda, Heterakidae)

doi: 10.3390/ani14182674

Figure Lengend Snippet: Maximum likelihood (Tamura Nei + Gamma distribution parameter model) phylogeny using the mtCOI gene as the genetic marker. The numbers at the nodes indicate bootstrap support obtained through 1000 replications (ML/NJ). Only bootstrap values >50 are shown. Reference NCBI sequences are shown with their accession numbers. The representative sequences for H. spumosa and H. gallinarum are highlighted in ‘red’ and ‘blue’, respectively, with the host, location, and host ID given in parentheses. The clade divisions of H. spumosa are labeled accordingly.

Article Snippet: As an outgroup of the genus, we collected Heterakis from Gallus gallus (Linnaeus, 1758) collected in Udon Thani Province (Thailand), as well as H. gallinarum (GenBank accession number KP308348, KT310157, LC592777); H. beramporia Lane, 1914 (GenBank accession number: KU529971); H. dispar (Schrank, 1790) (GenBank accession number MF319969, NC042411, OM530143); H. isolonche Linstow, 1906 (GenBank accession number KM212953).

Techniques: Marker, Labeling

Journal: Animals : an Open Access Journal from MDPI

Article Title: Genetic Characterization of the Co-Invasive Rodent Parasite Heterakis spumosa (Nematoda, Heterakidae)

doi: 10.3390/ani14182674

Figure Lengend Snippet: Intraspecies genetic distances in H. spumosa and interspecies genetic distance between representatives of the genus Heterakis in birds.

Article Snippet: As an outgroup of the genus, we collected Heterakis from Gallus gallus (Linnaeus, 1758) collected in Udon Thani Province (Thailand), as well as H. gallinarum (GenBank accession number KP308348, KT310157, LC592777); H. beramporia Lane, 1914 (GenBank accession number: KU529971); H. dispar (Schrank, 1790) (GenBank accession number MF319969, NC042411, OM530143); H. isolonche Linstow, 1906 (GenBank accession number KM212953).

Techniques:

Journal: Microorganisms

Article Title: Dual-Emission Fluorescence Resonance Energy Transfer (FRET) PCR Discriminates Salmonella Pullorum and Gallinarum

doi: 10.3390/microorganisms12091815

Figure Lengend Snippet: Discrimination of S. Pullorum and S. Gallinarum through high-resolution melting curve analysis genotyping. The T m of probe hybridization to the targets was determined by high-resolution melting (HRM) curve analysis as the peak of the second derivative of the fluorescence released during a temperature increase from 38 °C to 85 °C. Fluorescence demonstrates unique and distinct T m differences between S. Pullorum (59.6 °C with wide shoulder ± standard deviation 0.17 °C), S. Gallinarum (56.2 °C ± standard deviation 0.16 °C), and negative control (no melting curve). When five concentrations of the targets (10 1 to 10 5 copies of gene/reaction system, illustrated in different lines) were used for each Salmonella strain, the peaks and shapes of the melting curves remained the same.

Article Snippet: Plasmids used for analytical sensitivity (LOD): Three plasmids (Integrated DNA Technologies, Coralville, IA, USA) containing portions of the pegB gene of S . Pullorum and S . Gallinarum were used as the positive controls and for quantitative standards (10 5 , 10 4 , 10 3 , 10 2 , 10 1 copies of pegB molecules/10 μL).

Techniques: Hybridization, Fluorescence, Standard Deviation, Negative Control