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Agilent technologies g2565ca dna microarray scanner
Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum"

Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkn1069

Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
Figure Legend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

Techniques Used: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

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Microarray:

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Expressing:

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Transformation Assay:

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Hybridization:

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Transfection:

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Indirect Immunoperoxidase Assay:

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Reverse Transcription Polymerase Chain Reaction:

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DNA Extraction:

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Isolation:

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Labeling:

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Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
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Article Title: IL-4 Up-Regulates MiR-21 and the MiRNAs Hosted in the CLCN5 Gene in Chronic Lymphocytic Leukemia
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Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Ionizing radiation response of primary normal human lens epithelial cells
Article Snippet: The Agilent Low Input Quick Amp Labeling Kit, One-Color was used for synthesis of Cy3-labeled cRNA from 0.1 μg of total RNA. .. Microarray slides were scanned with the Agilent G2565CA DNA Microarray Scanner, and scanned data were extracted with the Agilent Feature Extraction software.

Mouse Assay:

Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
Article Snippet: Mice were deeply anesthetized with isofluorane, killed by decapitation and the brain was quickly isolated and placed into ice-cold PBS. .. The resulting cRNA (0.6 μg) was hybridized to an Agilent SurePrint G3 Mouse GE 8x60K Microarray (Agilent) as per manufacturer's instructions and scanned by an Agilent G2565CA DNA Microarray Scanner (Agilent).

Article Title: Reduced oxidative capacity in macrophages results in systemic insulin resistance
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Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: RNA samples from SBC isolated from Dock10-KO mice and WT mice were used as tests, and a pooled sample composed of equimolar amounts of RNA from 5 KO mice was used as reference . .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Polymerase Chain Reaction:

Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
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Plasmid Preparation:

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
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Software:

Article Title: The transcriptional response of mouse spleen B cells to IL-4: Comparison to the response of human peripheral blood B cells
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Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum
Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution. .. The signal intensity data were extracted from the primary scanned images using the GenePix Pro 6.0 software (Axon Technologies).

Article Title: Reduced oxidative capacity in macrophages results in systemic insulin resistance
Article Snippet: .. Data were extracted on a G2565CA DNA Microarray Scanner using the Agilent Feature Extraction Software (Agilent Technologies) with default settings. ..

Article Title: IL-4 Up-Regulates MiR-21 and the MiRNAs Hosted in the CLCN5 Gene in Chronic Lymphocytic Leukemia
Article Snippet: After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner. .. Images were analyzed with the Agilent Feature Extraction software.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner. .. Datasets, expressed as log10 ratios for test versus (vs ) reference, were computed from images by the Agilent Feature Extraction software, using normalization by linear and Lowess methods.

Article Title: Ionizing radiation response of primary normal human lens epithelial cells
Article Snippet: .. Microarray slides were scanned with the Agilent G2565CA DNA Microarray Scanner, and scanned data were extracted with the Agilent Feature Extraction software. .. Gene ontology (GO) was analyzed with AmiGO ( http://amigo.geneontology.org/amigo ), and pathway analysis was conducted using KEGG (Kyoto Encyclopedia of Genes and Genomes, http://www.kegg.jp/kegg/ ) and IPA (Ingenuity Pathway Analysis, https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/ ).

Irradiation:

Article Title: Ionizing radiation response of primary normal human lens epithelial cells
Article Snippet: At 3 h after irradiation with sham, 0.5 or 4 Gy, and at 8 h after irradiation with sham or 0.5 Gy, culture supernatants were harvested, and cells were washed once with PBS– , trypsinized, suspended in culture supernatants, precipitated, washed twice with PBS– , precipitated and stored at –80°C. .. Microarray slides were scanned with the Agilent G2565CA DNA Microarray Scanner, and scanned data were extracted with the Agilent Feature Extraction software.

Functional Assay:

Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
Article Snippet: The resulting cRNA (0.6 μg) was hybridized to an Agilent SurePrint G3 Mouse GE 8x60K Microarray (Agilent) as per manufacturer's instructions and scanned by an Agilent G2565CA DNA Microarray Scanner (Agilent). .. The functional relevance of microarray data generated from LPA and serum-treated animals was analyzed using Ingenuity Pathways Analysis (Ingenuity Systems, www.ingenuity.com ).

Agarose Gel Electrophoresis:

Article Title: Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research
Article Snippet: We thawed the samples on ice and isolated DNA using the QIAamp Blood Mini Kit (QIAGEN), evaluating it spectrophotometrically and by agarose gel electrophoresis. .. Subsequently, we washed the slides and scanned them using an Agilent Technologies G2565CA DNA Microarray Scanner.

In Vitro:

Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
Article Snippet: RNA was first reverse transcribed into complementary DNA, and the template cDNA was then converted to Cy3-labeled complementary RNA by in vitro transcription using Agilent's Low Input Quick Amp Labeling Kit, one-color (Agilent, Santa Clara, CA, USA). .. The resulting cRNA (0.6 μg) was hybridized to an Agilent SurePrint G3 Mouse GE 8x60K Microarray (Agilent) as per manufacturer's instructions and scanned by an Agilent G2565CA DNA Microarray Scanner (Agilent).

Laser Capture Microdissection:

Article Title: LPA signaling initiates schizophrenia-like brain and behavioral changes in a mouse model of prenatal brain hemorrhage
Article Snippet: The PFC (Bregma's area 2.5–1.3 mm) and ventral tegmental area (Bregma's area −3.0 to −4.2 mm) were isolated quickly from the brain via microdissection in sterile PBS using a standard dissection stereomicroscope. .. The resulting cRNA (0.6 μg) was hybridized to an Agilent SurePrint G3 Mouse GE 8x60K Microarray (Agilent) as per manufacturer's instructions and scanned by an Agilent G2565CA DNA Microarray Scanner (Agilent).

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    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
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    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
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    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
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    93
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    g2565ca dna microarray scanner - by Bioz Stars, 2020-04
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    94
    Agilent technologies dna microarray scanner g2565ba
    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
    Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner g2565ba - by Bioz Stars, 2020-04
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    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: RNA Expression, Microarray, Expressing

    Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: Expressing, Microarray