xevo g2 s qtof  (Thermo Fisher)


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    Structured Review

    Thermo Fisher xevo g2 s qtof
    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters <t>Acquity–Xevo</t> <t>G2-S</t> <t>QTOF,</t> Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.
    Xevo G2 S Qtof, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xevo g2 s qtof/product/Thermo Fisher
    Average 93 stars, based on 5571 article reviews
    Price from $9.99 to $1999.99
    xevo g2 s qtof - by Bioz Stars, 2020-07
    93/100 stars

    Images

    1) Product Images from "Best practices and benchmarks for intact protein analysis for top-down mass spectrometry"

    Article Title: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

    Journal: Nature Methods

    doi: 10.1038/s41592-019-0457-0

    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.
    Figure Legend Snippet: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    2) Product Images from "Best practices and benchmarks for intact protein analysis for top-down mass spectrometry"

    Article Title: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

    Journal: Nature Methods

    doi: 10.1038/s41592-019-0457-0

    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.
    Figure Legend Snippet: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    3) Product Images from "Best practices and benchmarks for intact protein analysis for top-down mass spectrometry"

    Article Title: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

    Journal: Nature Methods

    doi: 10.1038/s41592-019-0457-0

    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.
    Figure Legend Snippet: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    4) Product Images from "Best practices and benchmarks for intact protein analysis for top-down mass spectrometry"

    Article Title: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

    Journal: Nature Methods

    doi: 10.1038/s41592-019-0457-0

    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.
    Figure Legend Snippet: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy

    Related Articles

    High Performance Liquid Chromatography:

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    Article Snippet: .. The extract was filtered and analysed by means of HPLC-HRMS (Exactive Plus Orbitrap Mass Spectrometer, Thermo Scientific) operating with a 150 × 2.1 mm 3 μ Betasil C18 column (Thermo Scientific) and a gradient from 5 to 98% solvent B in 15 min (solvent A, 0.3% (v/v ) HCOOH in water; solvent B, 0.1% (v/v ) HCOOH in acetonitrile) at a flow rate of 200 μl min−1 and UV/HRMS detection. ..

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    Flow Cytometry:

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    Mass Spectrometry:

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    Liquid Chromatography with Mass Spectroscopy:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Tandem Mass Spectroscopy:

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

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    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

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    Thermo Fisher ft icr orbitrap lc ms ms system
    Ft Icr Orbitrap Lc Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ft icr orbitrap lc ms ms system/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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