incubator  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Forma Series II 3110 Water Jacketed CO2 Incubator
    Description:
    Precisely control the CO2 and temperature with Thermo Scientific Forma Series II 3110 Water Jacketed CO2 Incubators which combine precise control with a choice of TC or IR sensors Featuring superior parameter recovery characteristics with innovative continuous contamination control technology
    Catalog Number:
    3110
    Price:
    None
    Applications:
    CO2 Incubators|Lab Equipment
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher incubator
    Precisely control the CO2 and temperature with Thermo Scientific Forma Series II 3110 Water Jacketed CO2 Incubators which combine precise control with a choice of TC or IR sensors Featuring superior parameter recovery characteristics with innovative continuous contamination control technology
    https://www.bioz.com/result/incubator/product/Thermo Fisher
    Average 90 stars, based on 1179 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-02
    90/100 stars

    Images

    Related Articles

    Diagnostic Assay:

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: This strain was clinically isolated from a urethral swab of a patient with non-gonococcal urethritis, submitted to the Microbiology Laboratory of Sant’Orsola-Malpighi Hospital of Bologna, for routine diagnostic procedures and belongs to the laboratory collection. .. HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States).

    Acetylene Reduction Assay:

    Article Title: Na-K-Cl Cotransporter Contributes to Glutamate-Mediated Excitotoxicity
    Article Snippet: Live cells (6.25 × 10 5 ) were added to each well of a poly-D -lysine-coated 24-well plate and incubated at 37°C in an incubator (model 3110, Thermo Forma, Marietta, OH) with 5% CO2 and atmospheric air. .. After 96 hr in culture, cultures were refed with EMEM containing 4 μ m cytosine arabinofuranoside (Ara-c) for 72 hr.

    Cell Cycle Assay:

    Article Title: Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells
    Article Snippet: Briefly, cells were seeded (1x 105 cells/ T25 flask) and cultured in DMEM (with 10 % FBS and 1 % antibiotic-antimycotic solution) at 37 °C in CO2 incubator (Thermo scientific, forma series II 3110, USA). .. MTT (cell density of 5.0 x 103 cells/well in 96 well plate) and LDH assays (1 x 105 cells/ well in 6 well plate), mitochondrial membrane potential assay, DCFDA and AO-EB staining, cell cycle analysis and Annexin V-PI staining assays were performed by standardized protocols.

    Quantitative RT-PCR:

    Article Title: MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling
    Article Snippet: The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (31800-022, HyClone, UT, U.S.A.) containing 10% inactivated fetal bovine serum (FBS; 16000-044, Gibco Company, Grand Island, NY, U.S.A.) and 100 units/ml penicillin-streptomycin (15140122, Company, Grand Island, NY, U.S.A.) in a 5% CO2 incubator (3110 water jacket series, Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.) at 37°C. .. The expression of miR-140-5p was subsequently determined through the application of reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and the cell line with the lowest miR-140-5p expression versus that of GES-1 cells was selected for subsequent experiments, and another cell line (MGC-803) was selected for verification of the results.

    HAC Assay:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: The basic experimental procedure was that 0.5 mL of native α-MC or modified form was reacted with 0.5 mL, 40 mM NAD+ in pH 3.0, 0.2 M HAc-NaAc buffer at 55 °C for 30 min. Then the reaction was terminated by treating at 100 °C for 3 min. .. Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA).

    Incubation:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: The reactive mixture was centrifugated at 12000 g for 2 min. 0.5 mL of supernatant was incubated with 2 mL coupled-enzymes color developing solution at 37 °C for 30 min. Absorbance at 555 nm was determined by UV-VIS spectrophotometry. .. Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA).

    Article Title: Inhibiting the Na+/H+ exchanger reduces reperfusion injury: a small animal MRI study
    Article Snippet: .. The cells were incubated in 1 ml of OGD solution for 2 hours in a hypoxic incubator (model 3130, Thermo Forma) containing 94% N2 , 1% O2 , and 5% CO2 . .. Normoxic control cells were incubated for 2 hours in 5% CO2 and atmospheric air in a buffer identical to the OGD solution except for the addition of 5.5 mM glucose.

    Article Title: Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells
    Article Snippet: .. All cell cultures were incubated in a Forma™ Series II 3110 Water-Jacketed CO 2 Incubator (Thermo Fisher Scientific, USA) with 96% humidity and 5% CO2 enriched atmosphere. ..

    Article Title: Sustained Na+/H+ Exchanger Activation Promotes Gliotransmitter Release from Reactive Hippocampal Astrocytes following Oxygen-Glucose Deprivation
    Article Snippet: .. The cells were incubated in the OGD solution for 2 h in a hypoxic incubator (model 3130, Thermo Forma; Asheville NC) containing 94% N2 , 1% O2 , and 5% CO2 . .. Normoxic control cells were incubated for 2 h in 5% CO2 and atmospheric air in a buffer identical to the OGD solution except for the addition of 5.5 mM glucose.

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Transfected cells were seeded into 384 well plates, and incubated overnight in DMEM media supplemented with Fetal Bovine Serum (Invitrogen) at 37 ° C and 5% CO2 .

    Article Title: Na-K-Cl Cotransporter Contributes to Glutamate-Mediated Excitotoxicity
    Article Snippet: .. Cells were incubated in 0.5 ml of the OGD solution in a hypoxic incubator (model 3130, Thermo Forma) containing 94% N2 , 1%O2 , and 5% CO2 . .. The oxygen level in the medium of cultured cells in 24-well plates was monitored with an oxygen probe (model M1-730, Microelectrodes, Bedford, NH) and decreased to ∼2–3% after 60 min in the hypoxic incubator.

    Article Title: Na-K-Cl Cotransporter Contributes to Glutamate-Mediated Excitotoxicity
    Article Snippet: .. Live cells (6.25 × 10 5 ) were added to each well of a poly-D -lysine-coated 24-well plate and incubated at 37°C in an incubator (model 3110, Thermo Forma, Marietta, OH) with 5% CO2 and atmospheric air. .. After 96 hr in culture, cultures were refed with EMEM containing 4 μ m cytosine arabinofuranoside (Ara-c) for 72 hr.

    Article Title: Role of sodium/hydrogen exchanger isoform 1 in microglial activation and proinflammatory responses in ischemic brains
    Article Snippet: .. The cells were incubated in 1 ml of OGD solution for 2 h in a hypoxic incubator (model 3130; Thermo Forma) containing 94% N2 , 1% O2 , and 5% CO2 . .. Normoxic control cells were incubated for 2 h in 5% CO2 and atmospheric air in a buffer identical to the OGD solution except for the addition of 5.5 mM glucose.

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States). .. For the preparation of EBs, confluent HeLa cells were infected with CT in DMEM medium supplemented with cycloheximide 1 μg/mL, centrifuged at 640 × g for 2 h to facilitate cell penetration, then incubated at 37°C with 5% CO2 for 48 h ( ).

    Article Title: Overlapping Functions between SWR1 Deletion and H3K56 Acetylation in Candida albicans
    Article Snippet: .. For CO2 assays, plates were incubated in a Thermo Forma series II water-jacketed CO2 incubator at 25°C with 5% CO2 . ..

    Activity Assay:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: Paragraph title: Activity assay for mono-PEGylated α-MC ... Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA).

    Infection:

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States). .. For the preparation of EBs, confluent HeLa cells were infected with CT in DMEM medium supplemented with cycloheximide 1 μg/mL, centrifuged at 640 × g for 2 h to facilitate cell penetration, then incubated at 37°C with 5% CO2 for 48 h ( ).

    Expressing:

    Article Title: MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling
    Article Snippet: The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (31800-022, HyClone, UT, U.S.A.) containing 10% inactivated fetal bovine serum (FBS; 16000-044, Gibco Company, Grand Island, NY, U.S.A.) and 100 units/ml penicillin-streptomycin (15140122, Company, Grand Island, NY, U.S.A.) in a 5% CO2 incubator (3110 water jacket series, Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.) at 37°C. .. The expression of miR-140-5p was subsequently determined through the application of reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and the cell line with the lowest miR-140-5p expression versus that of GES-1 cells was selected for subsequent experiments, and another cell line (MGC-803) was selected for verification of the results.

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Cells were transiently transfected with 1 µg each of plasmid expressing GCaMP6s, Gqα15, and a test receptor using Lipofectamine 2000 (Invitrogen).

    Modification:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: Blank tubes was native α-MC or modified which were heated at 100 °C for 3 min. Other operation process was the same as sample. .. Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA).

    Article Title: Overlapping Functions between SWR1 Deletion and H3K56 Acetylation in Candida albicans
    Article Snippet: Stable white or opaque cells from log-phase cultures were spread onto agarose plates of Lee's medium (modified from reference ) supplemented with 25 μg/ml uridine and 50 μg/ml arginine, with 1.25% dextrose or N -acetyl- d -glucosamine (Spectrum Chemical) as the carbon source. .. For CO2 assays, plates were incubated in a Thermo Forma series II water-jacketed CO2 incubator at 25°C with 5% CO2 .

    Transfection:

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Cells were transiently transfected with 1 µg each of plasmid expressing GCaMP6s, Gqα15, and a test receptor using Lipofectamine 2000 (Invitrogen).

    Cell Culture:

    Article Title: Anti-angiogenic effects of resveratrol mediated by decreased VEGF and increased TSP1 expression in melanoma-endothelial cell co-culture
    Article Snippet: Paragraph title: Cell culture ... Hypoxic conditions were generated in Forma Series II water jacketed incubator (Thermo Fisher Scientific, Marietta, OH) set at 2% O2 , 5% CO2 , and balance N2 .

    Article Title: Mesenchymal Stem Cells from Human Umbilical Cord Express Preferentially Secreted Factors Related to Neuroprotection, Neurogenesis, and Angiogenesis
    Article Snippet: Cortices were dissected mechanically from embryonic rat brains at E17; these were then triturated, filtered, centrifuged in HBSS, and cultured at 37°C in Neurobasal medium (Invitrogen) supplemented with 10% B27 (Invitrogen), 100 U/ml penicillin and 100 mg/ml streptomycin with 5% CO2 . .. For the OGD, the cultures were placed into a hypoxic incubator (Thermo Scientific model 3130) on day 4 at 1% O2 with a gas mixture of 95% N2/5% CO2 , and the medium was replaced with glucose-free DMEM (Gibco 11966-025) for 24 hours.

    Article Title: MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling
    Article Snippet: .. The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (31800-022, HyClone, UT, U.S.A.) containing 10% inactivated fetal bovine serum (FBS; 16000-044, Gibco Company, Grand Island, NY, U.S.A.) and 100 units/ml penicillin-streptomycin (15140122, Company, Grand Island, NY, U.S.A.) in a 5% CO2 incubator (3110 water jacket series, Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.) at 37°C. .. The expression of miR-140-5p was subsequently determined through the application of reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and the cell line with the lowest miR-140-5p expression versus that of GES-1 cells was selected for subsequent experiments, and another cell line (MGC-803) was selected for verification of the results.

    Article Title: Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells
    Article Snippet: Paragraph title: Cell culture and media ... All cell cultures were incubated in a Forma™ Series II 3110 Water-Jacketed CO 2 Incubator (Thermo Fisher Scientific, USA) with 96% humidity and 5% CO2 enriched atmosphere.

    Article Title: Na-K-Cl Cotransporter Contributes to Glutamate-Mediated Excitotoxicity
    Article Snippet: Cells were incubated in 0.5 ml of the OGD solution in a hypoxic incubator (model 3130, Thermo Forma) containing 94% N2 , 1%O2 , and 5% CO2 . .. The oxygen level in the medium of cultured cells in 24-well plates was monitored with an oxygen probe (model M1-730, Microelectrodes, Bedford, NH) and decreased to ∼2–3% after 60 min in the hypoxic incubator.

    Article Title: Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells
    Article Snippet: .. Briefly, cells were seeded (1x 105 cells/ T25 flask) and cultured in DMEM (with 10 % FBS and 1 % antibiotic-antimycotic solution) at 37 °C in CO2 incubator (Thermo scientific, forma series II 3110, USA). ..

    Article Title: Curcumin-mediated bone marrow mesenchymal stem cell sheets create a favorable immune microenvironment for adult full-thickness cutaneous wound healing
    Article Snippet: .. BMSC sheets were cultured in OriCell™ mouse MSC Growth Medium (MUCMX-90011, Cyagen Biosciences, Inc.) with 100 mg/mL vitamin C in a humidified incubator (Thermo Scientific Forma 3110, Thermo Fisher Scientific, Inc.) at 37 °C with 5% CO2 . ..

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States). .. CT was propagated in HeLa cells, cultured in Dulbecco’s minimal essential medium (DMEM) (EuroClone, Pero, Italy), supplemented with 10% fetal bovine serum, 1% L -glutamine 200 mM, and antibiotics (vancomycin 10 mg/L, gentamicin 10 mg/L, and amphotericin B 0.3 mg/L).

    Generated:

    Article Title: Anti-angiogenic effects of resveratrol mediated by decreased VEGF and increased TSP1 expression in melanoma-endothelial cell co-culture
    Article Snippet: .. Hypoxic conditions were generated in Forma Series II water jacketed incubator (Thermo Fisher Scientific, Marietta, OH) set at 2% O2 , 5% CO2 , and balance N2 . .. Fluorescent labeling of endothelial cells The retroviral vector pBMN-mcherry (Provided as a gift by Dr. Steven George, University of California Irvine, Irvine, CA) was used to transduce HMVECad endothelial cells with RFP for two consecutive days as described previously [ ].

    Sonication:

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States). .. HeLa cells were then detached and fragmented by sonication, by using a Bandelin sonicator at minimum power.

    MTT Assay:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA). .. MTT assay was used to detect the inhibitory effect of α-MC and mPEGylated α-MC.

    Article Title: Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells
    Article Snippet: Briefly, cells were seeded (1x 105 cells/ T25 flask) and cultured in DMEM (with 10 % FBS and 1 % antibiotic-antimycotic solution) at 37 °C in CO2 incubator (Thermo scientific, forma series II 3110, USA). .. MTT (cell density of 5.0 x 103 cells/well in 96 well plate) and LDH assays (1 x 105 cells/ well in 6 well plate), mitochondrial membrane potential assay, DCFDA and AO-EB staining, cell cycle analysis and Annexin V-PI staining assays were performed by standardized protocols.

    Fluorescence:

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Cells were imaged in reading buffer [Hanks’s Balanced Salt Solution (GIBCO) + 20 mM HEPES (Sigma-Aldrich)] using GFP-channel fluorescence of a Hamamatsu FDSS-6000 kinetic plate reader at The Rockefeller University High-Throughput Screening Resource Center.

    Multiple Displacement Amplification:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: .. Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA). .. MTT assay was used to detect the inhibitory effect of α-MC and mPEGylated α-MC.

    Isolation:

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: This strain was clinically isolated from a urethral swab of a patient with non-gonococcal urethritis, submitted to the Microbiology Laboratory of Sant’Orsola-Malpighi Hospital of Bologna, for routine diagnostic procedures and belongs to the laboratory collection. .. HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States).

    Purification:

    Article Title: Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
    Article Snippet: Paragraph title: C. trachomatis Propagation and Purification of EBs ... HeLa cells (ATCC CCL-2), a cell line originated from a human cervix carcinoma, were grown to confluent monolayers in 5% CO2 at 37°C, inside a Forma Series II 3110 Water-Jacketed CO2 Incubator (Thermo Fisher Scientific, Waltham, MA, United States).

    Plasmid Preparation:

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Cells were transiently transfected with 1 µg each of plasmid expressing GCaMP6s, Gqα15, and a test receptor using Lipofectamine 2000 (Invitrogen).

    Real-time Polymerase Chain Reaction:

    Article Title: MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling
    Article Snippet: The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (31800-022, HyClone, UT, U.S.A.) containing 10% inactivated fetal bovine serum (FBS; 16000-044, Gibco Company, Grand Island, NY, U.S.A.) and 100 units/ml penicillin-streptomycin (15140122, Company, Grand Island, NY, U.S.A.) in a 5% CO2 incubator (3110 water jacket series, Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.) at 37°C. .. The expression of miR-140-5p was subsequently determined through the application of reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and the cell line with the lowest miR-140-5p expression versus that of GES-1 cells was selected for subsequent experiments, and another cell line (MGC-803) was selected for verification of the results.

    Selection:

    Article Title: Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells
    Article Snippet: CD OptiCHO® medium (Gibco, USA), lacking HT, was used as the selection medium and was supplemented with L-Glutamine (1 mM, Sigma-Aldrich Co., USA) and selection antibiotic G418 (250 μg/mL, InvivoGen, USA). .. All cell cultures were incubated in a Forma™ Series II 3110 Water-Jacketed CO 2 Incubator (Thermo Fisher Scientific, USA) with 96% humidity and 5% CO2 enriched atmosphere.

    Spectrophotometry:

    Article Title: Chemosynthesis and characterization of site-specific N-terminally PEGylated Alpha-momorcharin as apotential agent
    Article Snippet: The reactive mixture was centrifugated at 12000 g for 2 min. 0.5 mL of supernatant was incubated with 2 mL coupled-enzymes color developing solution at 37 °C for 30 min. Absorbance at 555 nm was determined by UV-VIS spectrophotometry. .. Three different tumor cell lines which are sensitive to α-MC, including human cervix adenocarcinoma Hela cell line, human lung adenocarcinoma A549 cell line and human breast adenocarcinoma MDA-MB-231 cell line were maintained in DMEM culture medium and supplemented with 10.0% fetal bovine serum containing 100 U/mL penicillin and 50 U/mL streptomycin in a 5.0% CO2 incubator at 37.0 °C (Thermo Forma 3110, USA).

    Concentration Assay:

    Article Title: Inhibiting the Na+/H+ exchanger reduces reperfusion injury: a small animal MRI study
    Article Snippet: This solution has a K+ concentration (~ 5.8 mM) which is similar to the neurobasal medium (5.6 mM) used for cell cultures. .. The cells were incubated in 1 ml of OGD solution for 2 hours in a hypoxic incubator (model 3130, Thermo Forma) containing 94% N2 , 1% O2 , and 5% CO2 .

    High Throughput Screening Assay:

    Article Title: A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito
    Article Snippet: HEK-293T cells were maintained using standard protocols in a Thermo Scientific FORMA Series II – Water Jacketed CO2 incubator. .. Cells were imaged in reading buffer [Hanks’s Balanced Salt Solution (GIBCO) + 20 mM HEPES (Sigma-Aldrich)] using GFP-channel fluorescence of a Hamamatsu FDSS-6000 kinetic plate reader at The Rockefeller University High-Throughput Screening Resource Center.

    Staining:

    Article Title: Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells
    Article Snippet: Briefly, cells were seeded (1x 105 cells/ T25 flask) and cultured in DMEM (with 10 % FBS and 1 % antibiotic-antimycotic solution) at 37 °C in CO2 incubator (Thermo scientific, forma series II 3110, USA). .. MTT (cell density of 5.0 x 103 cells/well in 96 well plate) and LDH assays (1 x 105 cells/ well in 6 well plate), mitochondrial membrane potential assay, DCFDA and AO-EB staining, cell cycle analysis and Annexin V-PI staining assays were performed by standardized protocols.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher incubator
    Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Thermo Fisher
    Average 90 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    89
    Thermo Fisher n2
    N2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n2/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n2 - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    90
    Thermo Fisher co2 incubator
    Co2 Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Thermo Fisher
    Average 90 stars, based on 918 article reviews
    Price from $9.99 to $1999.99
    co2 incubator - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results