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Effect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult <t>preadipocyte</t> (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a ), representative ORO stain images of fetal ( B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs ( B3 ; optimized medium) after 8 days of differentiation ( b ), and differentiation medium details ( c ). Scale bar: 50 μm. BM: basal medium, Dex : dexamethasone, DM : differentiation medium, Ins : insulin, oPADs: ovine preadipocytes, Rosi : rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences ( P < 0.05)
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Effect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult <t>preadipocyte</t> (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a ), representative ORO stain images of fetal ( B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs ( B3 ; optimized medium) after 8 days of differentiation ( b ), and differentiation medium details ( c ). Scale bar: 50 μm. BM: basal medium, Dex : dexamethasone, DM : differentiation medium, Ins : insulin, oPADs: ovine preadipocytes, Rosi : rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences ( P < 0.05)
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Effect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult preadipocyte (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a ), representative ORO stain images of fetal ( B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs ( B3 ; optimized medium) after 8 days of differentiation ( b ), and differentiation medium details ( c ). Scale bar: 50 μm. BM: basal medium, Dex : dexamethasone, DM : differentiation medium, Ins : insulin, oPADs: ovine preadipocytes, Rosi : rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences ( P < 0.05)

Journal: Cellular & Molecular Biology Letters

Article Title: PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes

doi: 10.1186/s11658-017-0037-1

Figure Lengend Snippet: Effect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult preadipocyte (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a ), representative ORO stain images of fetal ( B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs ( B3 ; optimized medium) after 8 days of differentiation ( b ), and differentiation medium details ( c ). Scale bar: 50 μm. BM: basal medium, Dex : dexamethasone, DM : differentiation medium, Ins : insulin, oPADs: ovine preadipocytes, Rosi : rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences ( P < 0.05)

Article Snippet: After six days of culture, cells were frozen (Preadipocyte Cryopreservation Medium, FM-1-100, Zen-Bio) and stored in liquid nitrogen until further use.

Techniques: Staining

Preadipocyte and adipogenic markers mRNA expression in murine and ovine primary cultured cells and cell lines. a ) DLK1 and ZFP423 mRNA expression in ovine fetal preadipocytes (oPADs). b ZFP423 mRNA expression in undifferentiated 3T3-L1 and NIH-3T3 cell lines and fetal ovine primary cultured cells, oPADs and fetal skin fibroblasts (oSFs). c Oil red O stain of 3T3-L1 cell line (preadipocyte), NIH-3T3 cell line (fibroblast), ovine female fetal preadipocytes (oPADs), and skin fibroblasts (oSFs) after differentiation induction for 8 days. Three fetal oPADs and three fetal oSFs primary cells (passage 3) from three different fetuses were used

Journal: Cellular & Molecular Biology Letters

Article Title: PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes

doi: 10.1186/s11658-017-0037-1

Figure Lengend Snippet: Preadipocyte and adipogenic markers mRNA expression in murine and ovine primary cultured cells and cell lines. a ) DLK1 and ZFP423 mRNA expression in ovine fetal preadipocytes (oPADs). b ZFP423 mRNA expression in undifferentiated 3T3-L1 and NIH-3T3 cell lines and fetal ovine primary cultured cells, oPADs and fetal skin fibroblasts (oSFs). c Oil red O stain of 3T3-L1 cell line (preadipocyte), NIH-3T3 cell line (fibroblast), ovine female fetal preadipocytes (oPADs), and skin fibroblasts (oSFs) after differentiation induction for 8 days. Three fetal oPADs and three fetal oSFs primary cells (passage 3) from three different fetuses were used

Article Snippet: After six days of culture, cells were frozen (Preadipocyte Cryopreservation Medium, FM-1-100, Zen-Bio) and stored in liquid nitrogen until further use.

Techniques: Expressing, Cell Culture, Staining