fluorogenic adam10 substrate (Biozyme Laboratories)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Biozyme Laboratories fluorogenic adam10 substrate
Fluorogenic Adam10 Substrate, supplied by Biozyme Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+adam10+substrate/adam10+fluorogenic+substrate/pm42006582-140-55-59
Average 86 stars, based on 1 article reviews

fluorogenic adam10 substrate - by Bioz Stars, 2026-09

86/100 stars

Images

Isolation:

Article Title: ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular Vesicles.
Article Snippet: .. Isolated cortical culture-derived sEVs were incubated at 37◦C with a fluorogenic ADAM10 substrate (‘PEPDAB063’, BioZyme, Inc.) in the presence or absence of ADAM10 inhibitor GI254023X (GI). (A) Schematic representation of the fluorogenic substrate assay, where ADAM10-mediated cleavage of the peptide separates the quencher (Dabcyl) from the fluorophore (5-FAM), resulting in increased fluorescence emission (~530 nm). (B) sEV-ADAM10 activity measurement was performed at 0.5, 3, and 24 h, with or without GI. ..

Article Title: ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular Vesicles.
Article Snippet: .. As protease activity is the result of a finely tuned interplay of many different factors and the mere presence of mature ADAM10 on EVs does not per se guarantee its activity there (Aljohmani et al., 2022 ; Lambrecht et al., 2018 ), we performed cell-free activity assays with the purified sEVs using a synthetic and fluorogenic ADAM10 substrate (PEPDAB063, BioZyme, Inc.) in the presence or absence of the selective ADAM10 inhibitor GI254023X (GI) (Figure 2A ). sEVs were isolated from cultured cortical cells as before but in the absence of a PI cocktail in the cell supernatants. ..

Incubation:

Fluorescence:

Activity Assay:

Article Title: ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular Vesicles.
Article Snippet: .. As protease activity is the result of a finely tuned interplay of many different factors and the mere presence of mature ADAM10 on EVs does not per se guarantee its activity there (Aljohmani et al., 2022 ; Lambrecht et al., 2018 ), we performed cell-free activity assays with the purified sEVs using a synthetic and fluorogenic ADAM10 substrate (PEPDAB063, BioZyme, Inc.) in the presence or absence of the selective ADAM10 inhibitor GI254023X (GI) (Figure 2A ). sEVs were isolated from cultured cortical cells as before but in the absence of a PI cocktail in the cell supernatants. ..

Purification:

Article Title: ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular Vesicles.
Article Snippet: .. As protease activity is the result of a finely tuned interplay of many different factors and the mere presence of mature ADAM10 on EVs does not per se guarantee its activity there (Aljohmani et al., 2022 ; Lambrecht et al., 2018 ), we performed cell-free activity assays with the purified sEVs using a synthetic and fluorogenic ADAM10 substrate (PEPDAB063, BioZyme, Inc.) in the presence or absence of the selective ADAM10 inhibitor GI254023X (GI) (Figure 2A ). sEVs were isolated from cultured cortical cells as before but in the absence of a PI cocktail in the cell supernatants. ..

Cell Culture:

Article Title: ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular Vesicles.
Article Snippet: .. As protease activity is the result of a finely tuned interplay of many different factors and the mere presence of mature ADAM10 on EVs does not per se guarantee its activity there (Aljohmani et al., 2022 ; Lambrecht et al., 2018 ), we performed cell-free activity assays with the purified sEVs using a synthetic and fluorogenic ADAM10 substrate (PEPDAB063, BioZyme, Inc.) in the presence or absence of the selective ADAM10 inhibitor GI254023X (GI) (Figure 2A ). sEVs were isolated from cultured cortical cells as before but in the absence of a PI cocktail in the cell supernatants. ..

Review