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fluorescent megamix plus ssc calibration beads  (BIOCYTEX Inc)

 
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    BIOCYTEX Inc fluorescent megamix plus ssc calibration beads
    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. <t>Megamix-Plus</t> FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.
    Fluorescent Megamix Plus Ssc Calibration Beads, supplied by BIOCYTEX Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent megamix plus ssc calibration beads/product/BIOCYTEX Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent megamix plus ssc calibration beads - by Bioz Stars, 2024-12
    86/100 stars

    Images

    1) Product Images from "Extracellular Vesicles from NSC-34 MN-like Cells Transfected with Mutant SOD1 Modulate Inflammatory Status of Raw 264.7 Macrophages"

    Article Title: Extracellular Vesicles from NSC-34 MN-like Cells Transfected with Mutant SOD1 Modulate Inflammatory Status of Raw 264.7 Macrophages

    Journal: Genes

    doi: 10.3390/genes15060735

    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. Megamix-Plus FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.
    Figure Legend Snippet: Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. Megamix-Plus FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.

    Techniques Used: Isolation, Flow Cytometry, Staining, Control



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    fluorescent megamix plus ssc calibration beads  (BIOCYTEX Inc)
    86
    BIOCYTEX Inc fluorescent megamix plus ssc calibration beads
    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. <t>Megamix-Plus</t> FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.
    Fluorescent Megamix Plus Ssc Calibration Beads, supplied by BIOCYTEX Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent megamix plus ssc calibration beads/product/BIOCYTEX Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent megamix plus ssc calibration beads - by Bioz Stars, 2024-12
    86/100 stars
    		  Buy from Supplier
    fluorescent calibrated beads megamix plus ssc  (BIOCYTEX Inc)
    86
    BIOCYTEX Inc fluorescent calibrated beads megamix plus ssc
    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. <t>Megamix-Plus</t> FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.
    Fluorescent Calibrated Beads Megamix Plus Ssc, supplied by BIOCYTEX Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent calibrated beads megamix plus ssc/product/BIOCYTEX Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent calibrated beads megamix plus ssc - by Bioz Stars, 2024-12
    86/100 stars
    		  Buy from Supplier
    megamix plus ssc fluorescent calibration beads  (BIOCYTEX Inc)
    86
    BIOCYTEX Inc megamix plus ssc fluorescent calibration beads
    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. <t>Megamix-Plus</t> FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.
    Megamix Plus Ssc Fluorescent Calibration Beads, supplied by BIOCYTEX Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix plus ssc fluorescent calibration beads/product/BIOCYTEX Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix plus ssc fluorescent calibration beads - by Bioz Stars, 2024-12
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. Megamix-Plus FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.

    Journal: Genes

    Article Title: Extracellular Vesicles from NSC-34 MN-like Cells Transfected with Mutant SOD1 Modulate Inflammatory Status of Raw 264.7 Macrophages

    doi: 10.3390/genes15060735

    Figure Lengend Snippet: Quantitative analysis of large and small EVs isolated from mSOD1 NSC-34 MN-like cells using high-resolution flow cytometry. ( A ): Flow cytometry gating strategy for phenotyping large and small EVs. Megamix-Plus FSC is used to set FSC-vSSC for EV events. APC-Annexin V and tetraspanin APC-CD81 are used to stain isolated GFP-positive lEVs and sEVs, respectively. Before analysis on a CytoFLEX S flow cytometer, EVs are diluted 1:200 in 1X PBS. For all assays, 30 μL of the control (unstained EV) and 30 μL of each sample were measured at slow flow. ( B ), Histogram of GFP/Annexin V+ (lEVs) and GFP/CD81+ (sEVs) events in EV subtypes isolated from mSOD1 NSC-34. Graphs show mean ± SEM.

    Article Snippet: Fluorescent Megamix-Plus SSC calibration beads (BioCytex, Marseille, France) were used to optimize cytometer settings and to define the EV gate.

    Techniques: Isolation, Flow Cytometry, Staining, Control