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Olympus fluorescence signals
Fluorescence Signals, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Microscopy:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy. .. Ectopic expression of CsGAIP in Arabidopsis To make the CsGAIP overexpression construct, full length CsGAIP cDNA were cloned using primers 5′-GG ACTAGT ATGAAGAGGGAGCATCACCATCTTC-3′ (Spe I site in bold) and 5′-GACTGC CACG TG TCACTTAGCGACCACCGGGTT-3′ (Pml I site in bold), and inserted into the pCAMBIA1305.1 vector with 35S promoter.

Article Title: Single-molecule peptide fingerprinting
Article Snippet: Single-molecule fluorescence measurements were performed with a prism-type total internal reflection fluorescence microscope. .. Fluorescence signals from single molecules were collected through a 60× water immersion objective (UplanSApo; Olympus) with an inverted microscope (IX71; Olympus).

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: .. The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan). .. All digital images of CDX2 and Hoechst signals were acquired with the FSX-BSW software (Olympus) using the same contrast, brightness and exposure settings.

Article Title: Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function
Article Snippet: Then, the heart was mounted on the custom-made microscope stage, and AcGFP-expressing Z-disks were imaged. .. In this case, the heart was illuminated with a 532-nm laser light (PID-1500; Snake Creek Lasers), and the resultant fluorescence signals (emission filter, BA575IF; Olympus) were detected by the EMCCD camera.

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: .. Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan). .. All histological images captured using the same exposure conditions were analyzed with the Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA).

Article Title: Rescue from galactose-induced death of Leigh Syndrome patient cells by pyruvate and NAD+
Article Snippet: .. Next, fluorescence signals were visualized using the high-content microscopy system described above (2×2 image montage; 4 images in total) using a x20 objective (UApo/340, NA 0.75, Olympus). ..

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: .. The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser. .. RNA isolation and reverse transcription real-time polymerase chain reaction To detect KHK mRNA expressions, total RNA from the treated cells were isolated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.

Article Title: G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Article Snippet: .. Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan). .. Cytogenetic analysis To determine the presence of anaphase bridges, cells were seeded on glass coverslips in complete culture medium and treated with two enantiomers for 5 days, then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) and mounted.

Article Title: Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis
Article Snippet: .. The Drosophila JHAMT rabbit polyclonal antibody (1:100) [ ] and the FITC-conjugated Affinipure Goat Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch Inc.) were used, and the fluorescence signals were captured with an Olympus IX71 invert fluorescence microscope (Japan) [ , , ]. .. JH treatments and cell culture Methoprene (Service Chemical Inc., Germany), JH III (Sigma-Aldrich), and MF (Echelon) were purchased.

Article Title: Identification of novel Nrf2 activators from Cinnamomum chartophyllum H.W. Li and their potential application of preventing oxidative insults in human lung epithelial cells
Article Snippet: .. The fluorescence signals were imaged using Olympus BX53 fluorescence microscope coupled to Olympus DP73 digital camera (Tokyo, Japan). ..

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: .. In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70. .. Confocal imaging of whole-cell [Ca2+ ]i and Ca2+ sparks was performed using a Bio-Rad Radiance 2000 confocal system (Cambridge, MA, USA) coupled to a Nikon TE300 inverted microscope equipped with a Nikon 60 × oil immersion lens (NA = 1.4).

Clone Assay:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: Subcellular localization in cucumber protoplasts and onion epidermal cells For transient expression in cucumber protoplasts and onion epidermal cells , the full length coding region of CsGAIP were cloned using primers 5′-ACGC GTCGAC ATGAAGAGGGAGCATCACCATCTTC-3′ (Sal I site in bold) and 5′-CG GGATCC CTTAGCGACCACCGGGTTGTT-3′ (BamH I site in bold), and then inserted into the pEZS-NL vector (with GFP protein driven by 35S promoter) to generate 35S:GFP-CsGAIP , and the empty pEZS-NL vector was used a control. .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy.

Flow Cytometry:

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Data acquisition A syringe pump (KD Scientific, Holliston, MA) running in withdrawal mode at 0.15 ml min−1 was applied to the flow cell outlet to introduce TtAgo:guide complex (pre-heated to 23, 37, 45, or 55°C) supplemented with an oxygen scavenging system and triplet quenchers. .. Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M).

Immunohistochemistry:

Article Title: Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis
Article Snippet: Paragraph title: Immunohistochemistry ... The Drosophila JHAMT rabbit polyclonal antibody (1:100) [ ] and the FITC-conjugated Affinipure Goat Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch Inc.) were used, and the fluorescence signals were captured with an Olympus IX71 invert fluorescence microscope (Japan) [ , , ].

Inverted Microscopy:

Article Title: Single-molecule peptide fingerprinting
Article Snippet: .. Fluorescence signals from single molecules were collected through a 60× water immersion objective (UplanSApo; Olympus) with an inverted microscope (IX71; Olympus). .. Scattered light from the 532-nm and 633-nm laser beams was blocked by a triple-notch filter (NF01-488/532/635; Semrock).

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Imaging was performed on an IX81‐ZDC2 zero‐drift inverted microscope equipped with a cell^TIRF motorized multicolor TIRF illuminator with 405, 488, 561, and 640 nm 100 mW lasers and a 100× , oil immersion, 1.49 numerical aperture UAPON TIRF objective with FN = 22 (Olympus, Tokyo, Japan). .. Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M).

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: Photometry experiments were performed on the stage of an Olympus IX-70 inverted microscope. .. In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70.

Fluorescence:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy. .. Ectopic expression of CsGAIP in Arabidopsis To make the CsGAIP overexpression construct, full length CsGAIP cDNA were cloned using primers 5′-GG ACTAGT ATGAAGAGGGAGCATCACCATCTTC-3′ (Spe I site in bold) and 5′-GACTGC CACG TG TCACTTAGCGACCACCGGGTT-3′ (Pml I site in bold), and inserted into the pCAMBIA1305.1 vector with 35S promoter.

Article Title: Single-molecule peptide fingerprinting
Article Snippet: .. Fluorescence signals from single molecules were collected through a 60× water immersion objective (UplanSApo; Olympus) with an inverted microscope (IX71; Olympus). .. Scattered light from the 532-nm and 633-nm laser beams was blocked by a triple-notch filter (NF01-488/532/635; Semrock).

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: .. The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan). .. All digital images of CDX2 and Hoechst signals were acquired with the FSX-BSW software (Olympus) using the same contrast, brightness and exposure settings.

Article Title: Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function
Article Snippet: .. In this case, the heart was illuminated with a 532-nm laser light (PID-1500; Snake Creek Lasers), and the resultant fluorescence signals (emission filter, BA575IF; Olympus) were detected by the EMCCD camera. .. A 60× lens (60×W; numerical aperture [N/A] 1.00; LUMPlanFL N; Olympus) was used.

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: .. Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M). .. The primary image was relayed to two ImagEM X2 EM-CCD cameras (C9100–23B, Hamamatsu Photonics, Hamamatsu, Japan) using a Cairn three-way splitter equipped with a longpass dichroic mirror (T635lpxr-UF2, Chroma) and bandpass filters (Chroma 595/50) in front of the ‘‘green’’ camera.

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: .. Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan). .. All histological images captured using the same exposure conditions were analyzed with the Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA).

Article Title: Rescue from galactose-induced death of Leigh Syndrome patient cells by pyruvate and NAD+
Article Snippet: .. Next, fluorescence signals were visualized using the high-content microscopy system described above (2×2 image montage; 4 images in total) using a x20 objective (UApo/340, NA 0.75, Olympus). ..

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: .. The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser. .. RNA isolation and reverse transcription real-time polymerase chain reaction To detect KHK mRNA expressions, total RNA from the treated cells were isolated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.

Article Title: G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Article Snippet: .. Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan). .. Cytogenetic analysis To determine the presence of anaphase bridges, cells were seeded on glass coverslips in complete culture medium and treated with two enantiomers for 5 days, then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) and mounted.

Article Title: Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis
Article Snippet: .. The Drosophila JHAMT rabbit polyclonal antibody (1:100) [ ] and the FITC-conjugated Affinipure Goat Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch Inc.) were used, and the fluorescence signals were captured with an Olympus IX71 invert fluorescence microscope (Japan) [ , , ]. .. JH treatments and cell culture Methoprene (Service Chemical Inc., Germany), JH III (Sigma-Aldrich), and MF (Echelon) were purchased.

Article Title: Identification of novel Nrf2 activators from Cinnamomum chartophyllum H.W. Li and their potential application of preventing oxidative insults in human lung epithelial cells
Article Snippet: .. The fluorescence signals were imaged using Olympus BX53 fluorescence microscope coupled to Olympus DP73 digital camera (Tokyo, Japan). ..

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: .. In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70. .. Confocal imaging of whole-cell [Ca2+ ]i and Ca2+ sparks was performed using a Bio-Rad Radiance 2000 confocal system (Cambridge, MA, USA) coupled to a Nikon TE300 inverted microscope equipped with a Nikon 60 × oil immersion lens (NA = 1.4).

Mass Spectrometry:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy. .. Ectopic expression of CsGAIP in Arabidopsis To make the CsGAIP overexpression construct, full length CsGAIP cDNA were cloned using primers 5′-GG ACTAGT ATGAAGAGGGAGCATCACCATCTTC-3′ (Spe I site in bold) and 5′-GACTGC CACG TG TCACTTAGCGACCACCGGGTT-3′ (Pml I site in bold), and inserted into the pCAMBIA1305.1 vector with 35S promoter.

Isolation:

Article Title: Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function
Article Snippet: Paragraph title: Isolated heart experiment ... In this case, the heart was illuminated with a 532-nm laser light (PID-1500; Snake Creek Lasers), and the resultant fluorescence signals (emission filter, BA575IF; Olympus) were detected by the EMCCD camera.

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M). .. The TIRF imaging system was isolated from floor vibrations with a Micro‐g laboratory table (Technical Manufacturing Corporation, Peabody, MA).

Transferring:

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: For experiments that involved the simultaneous measurement of electrophysiological signals and [Ca2+ ]i , cells were loaded with the penta-potassium salt of Fluo-4 (50 μ m ) through the patch pipette. .. In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70.

Laser-Scanning Microscopy:

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan). .. Confocal digital images of the fluorescence signals of Ac-H3K9 and Ac-H4K12 were captured using a confocal laser-scanning microscope (Carl Zeiss, Germany).

Immunolabeling:

Article Title: G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Article Snippet: For immunolabeling, cells were incubated with primary antibody and then washed in phosphate buffered saline and incubated with the fluorophore-conjugated secondary antibodies. .. Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan).

Blocking Assay:

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: After washing with the blocking solution, the samples were stained with 10 mg/ml Hoechst 33258 (Sigma-Aldrich) for 10 min and mounted on slides in 50% glycerol in PBS. .. The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan).

Incubation:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy. .. Ectopic expression of CsGAIP in Arabidopsis To make the CsGAIP overexpression construct, full length CsGAIP cDNA were cloned using primers 5′-GG ACTAGT ATGAAGAGGGAGCATCACCATCTTC-3′ (Spe I site in bold) and 5′-GACTGC CACG TG TCACTTAGCGACCACCGGGTT-3′ (Pml I site in bold), and inserted into the pCAMBIA1305.1 vector with 35S promoter.

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: After washing extensively in the blocking solution, the samples were incubated with the secondary antibodies, Alexa Fluor 488-conjugated anti-rabbit IgG (1:500; Molecular Probes, Eugene, OR, USA) and Alexa Fluor 594-conjugated anti-mouse IgG (1:500; Molecular Probes), for 1 h at room temperature. .. The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan).

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: After washing three times with phosphate-buffered saline (PBS), the sections were incubated with the appropriate peroxidase-conjugated secondary antibodies anti-mouse IgG (1:1000, Cell Signaling Technology, Danvers, MA, USA) and anti-rabbit IgG (1:1000, Cell Signaling Technology) for 1 h at room temperature. .. Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan).

Article Title: Rescue from galactose-induced death of Leigh Syndrome patient cells by pyruvate and NAD+
Article Snippet: After this incubation, cells were washed twice with and placed in Dulbecco’s phosphate-buffered saline (DPBS containing calcium and magnesium, #14040133, Thermo Fisher). .. Next, fluorescence signals were visualized using the high-content microscopy system described above (2×2 image montage; 4 images in total) using a x20 objective (UApo/340, NA 0.75, Olympus).

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: After incubation in fluorescence conjugated IgG (Vector Laboratories, Burlingame, CA, USA) for 1 hour, the cells were rinsed with PBS. .. The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser.

Article Title: G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Article Snippet: For immunolabeling, cells were incubated with primary antibody and then washed in phosphate buffered saline and incubated with the fluorophore-conjugated secondary antibodies. .. Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan).

Article Title: Identification of novel Nrf2 activators from Cinnamomum chartophyllum H.W. Li and their potential application of preventing oxidative insults in human lung epithelial cells
Article Snippet: After 3 times washing in PBS, the cell climbing pieces were incubated with Alexa Flour 594 (Proteintech Group, IL, USA) for 1 h at room temperature in the darkness, and nuclei were covered with DAPI for 10 min. .. The fluorescence signals were imaged using Olympus BX53 fluorescence microscope coupled to Olympus DP73 digital camera (Tokyo, Japan).

Introduce:

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Data acquisition A syringe pump (KD Scientific, Holliston, MA) running in withdrawal mode at 0.15 ml min−1 was applied to the flow cell outlet to introduce TtAgo:guide complex (pre-heated to 23, 37, 45, or 55°C) supplemented with an oxygen scavenging system and triplet quenchers. .. Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M).

Expressing:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: Subcellular localization in cucumber protoplasts and onion epidermal cells For transient expression in cucumber protoplasts and onion epidermal cells , the full length coding region of CsGAIP were cloned using primers 5′-ACGC GTCGAC ATGAAGAGGGAGCATCACCATCTTC-3′ (Sal I site in bold) and 5′-CG GGATCC CTTAGCGACCACCGGGTTGTT-3′ (BamH I site in bold), and then inserted into the pEZS-NL vector (with GFP protein driven by 35S promoter) to generate 35S:GFP-CsGAIP , and the empty pEZS-NL vector was used a control. .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy.

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: .. The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser. .. RNA isolation and reverse transcription real-time polymerase chain reaction To detect KHK mRNA expressions, total RNA from the treated cells were isolated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.

Staining:

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: Paragraph title: Immunofluorescence staining ... The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan).

Article Title: Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function
Article Snippet: In some experiments, CellMask Orange (5 µg/ml in the above solution, 2 ml at a speed of ∼0.5 drop/s; Life Technologies) was used to stain the T-tubules in ventricular myocytes in the isolated heart. .. In this case, the heart was illuminated with a 532-nm laser light (PID-1500; Snake Creek Lasers), and the resultant fluorescence signals (emission filter, BA575IF; Olympus) were detected by the EMCCD camera.

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: Paragraph title: Immunofluorescence Staining ... Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan).

Article Title: Rescue from galactose-induced death of Leigh Syndrome patient cells by pyruvate and NAD+
Article Snippet: To this end, the cells were stained in DMEM medium (#A1443001; Thermo Fisher), which was supplemented with 10 mM HEPES (#15630080; Thermo Fisher) and contained either CM-H2 DCFDA (1 µM) or HEt (10 µM) for 30 min (37 °C, 5% CO2 ) in the dark. .. Next, fluorescence signals were visualized using the high-content microscopy system described above (2×2 image montage; 4 images in total) using a x20 objective (UApo/340, NA 0.75, Olympus).

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: Paragraph title: Immunofluorescence staining ... The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser.

Construct:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: The constructs were introduced into cucumber protoplasts using Huang's method . .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy.

Software:

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan). .. All digital images of CDX2 and Hoechst signals were acquired with the FSX-BSW software (Olympus) using the same contrast, brightness and exposure settings.

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M). .. Illumination and acquisition parameters were controlled with cell^TIRF and MetaMorph software (Molecular Devices, Sunnyvale, CA), respectively.

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan). .. All histological images captured using the same exposure conditions were analyzed with the Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA).

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70. .. This system was operated with a computer running Lasersharp 2000 (v. 4.0) software.

Immunofluorescence:

Article Title: An intracytoplasmic injection of deionized bovine serum albumin immediately after somatic cell nuclear transfer enhances full-term development of cloned mouse embryos
Article Snippet: Paragraph title: Immunofluorescence staining ... The fluorescence signals of CDX2 and Hoechst were observed using a fluorescence microscope (FSX100, Olympus, Tokyo, Japan).

Article Title: Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex
Article Snippet: Paragraph title: Immunofluorescence Staining ... Fluorescence signals were observed with an optical microscope (BX51; Olympus, Tokyo, Japan).

Article Title: An increase in adenosine-5'-triphosphate (ATP) content in rostral ventrolateral medulla is engaged in the high fructose diet-induced hypertension
Article Snippet: Paragraph title: Immunofluorescence staining ... The expression and distribution of fluorescence signals were detected under an Olympus Fluoview 1000 laser confocal microscope (Tokyo, Japan) equipped with a krypton/argon laser.

Article Title: G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Article Snippet: Paragraph title: Immunofluorescence ... Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan).

Article Title: Identification of novel Nrf2 activators from Cinnamomum chartophyllum H.W. Li and their potential application of preventing oxidative insults in human lung epithelial cells
Article Snippet: Paragraph title: Immunofluorescence ... The fluorescence signals were imaged using Olympus BX53 fluorescence microscope coupled to Olympus DP73 digital camera (Tokyo, Japan).

Plasmid Preparation:

Article Title: A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes
Article Snippet: The onion epidermal layers were prepared and bombarded, as previously described , with gold particles containing the plasmid using a Bio-Rad PDS-1000/He particle delivery system. .. After bombardment, the onion epidermises were placed on MS medium and incubated in darkness at 22°C for 24 h. Fluorescence signals were detected using Olympus BX 51 fluorescence microscopy.

Imaging:

Article Title: An automated Bayesian pipeline for rapid analysis of single-molecule binding data
Article Snippet: Imaging was performed on an IX81‐ZDC2 zero‐drift inverted microscope equipped with a cell^TIRF motorized multicolor TIRF illuminator with 405, 488, 561, and 640 nm 100 mW lasers and a 100× , oil immersion, 1.49 numerical aperture UAPON TIRF objective with FN = 22 (Olympus, Tokyo, Japan). .. Fluorescence signals were split with a main dichroic mirror (Olympus OSF-LFQUAD) and triple emission filter (Olympus U-CZ491561639M).

Article Title: Mechanisms underlying variations in excitation-contraction coupling across the mouse left ventricular free wall
Article Snippet: In this microscope, fluorescence signals were collected through a 40 × (NA = 1.35; Olympus) lens and detected by an IonOptix photometry system coupled to the IX-70. .. Confocal imaging of whole-cell [Ca2+ ]i and Ca2+ sparks was performed using a Bio-Rad Radiance 2000 confocal system (Cambridge, MA, USA) coupled to a Nikon TE300 inverted microscope equipped with a Nikon 60 × oil immersion lens (NA = 1.4).

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    Olympus fluorescence in situ hybridisation fish signals
    Biofunctional effects of EGFR inactivation on different cervical cancer cell lines. ( A ) Dual-colour fluorescence in situ <t>hybridisation</t> <t>(FISH)-validated</t> amplification of the EGFR gene in cervical cancer cell lines. The FISH analysis showing a homogeneously stained region in CaSki cells with gene amplification. ( B ) Western blot indicates the protein level of EGFR in each cell line. ( C ) Each cell line was treated with 10 μ mol l –1 AG1478 to inhibit EGFR function, and cell viability was measured with an MTT assay 98 h later. An equal amount of DMSO was used as a control. * P
    Fluorescence In Situ Hybridisation Fish Signals, supplied by Olympus, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Olympus gfp mcherry fluorescence signal
    Identification of the M.tb proteins interfering autophagy in host cells. ( A ) The PPI network between M.tb and autophagy pathway proteins of host cells. ( B ) Raw264.7 cells expressing <t>GFPph-mCherry-LC3</t> for autophagy assay was generated using CRISPR/Cas9 system. The stable cell line transfected either with an empty vector or with 38 M.tb genes in the M.tb -autophagy PPI network individually, were treated with rapamycin to induce autophagy. ( C ) Rv2427c and control plasmid were transfected to GFPph-mCherry-LC3 Raw264.7 stable cell line and induce autophagy by rapamycin. Transfection of Rv2427c significantly rescued the rapamycin-induced decrease of <t>GFP/mCherry</t> fluorescence ratio of the aggregation dots. Arrows indicate transfection positive cells. Arrows indicate transfection positive cells. ( D ) Quantification of GFP/mCherry fluorescence ratio of the aggregation dots ( n = 48). ( E ) Verification of the protein-protein interaction by BiFC assay. Rv2427c were co-transfected with WASP and the negative controls into HEK 293T cells, respectively. Cells were harvested 36h after transfection for confocal fluorescence microscopy-based image analysis. The interactions between Rv2427c and prey proteins allow re-formation of a bimolecular fluorescent complex. ( F ) Quantification of the fluorescence.
    Gfp Mcherry Fluorescence Signal, supplied by Olympus, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Olympus tdtomato fluorescence signal
    <t>tdTomato</t> is expressed in various regions throughout the brain that contain PVALB-expressing neurons “(A) tdTomato expression visualized with an antibody against RFP to enhance signals in relatively dimmer regions (GP, hippocampus, neocortex) for this low magnification montage. Note that tdTomato is expressed throughout the brain including the cortex, globus pallidus, thalamic reticular nucleus, hippocampus and cerebellum and also shows ectopic expression in ependymal cells around the fimbria. The signal appears dim in the striatum due to low number of PVALB-expressing neurons (1–2% of neurons). Scale bar 1 mm. (B) Magnified view of tdTomato expression (red) in PVALB-expressing neurons (green) in the thalamic reticular nucleus. Note the high degree of co-expression (yellow). Scale bars in B–D 100 µm. (C) Magnified view of the globus pallidus. (D) Magnified view of the cerebellum.
    Tdtomato Fluorescence Signal, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Olympus gfp fluorescence signal
    BAK1 and BKK1 robustly suppress the expression of SA biosynthesis-related genes SID2 and EDS5 . Seedlings are grown in dark (D) for 4 days then relocated to long-day condition (L) for the indicated days (4D+0L, 4D+1L, 4D+2L). Seedlings grown in dark for 5 or 6 days (5D+0L or 6D+0L) are analyzed as control. Quantitative RT-PCR assays indicate both SID2 and EDS5 are significantly up-regulated in bak1-4 bkk1-1 compared to those in Col-0 (A) . <t>SID2-GFP</t> and <t>EDS5-YFP</t> are detected to locate to the entire chloroplast and chloroplast surface, respectively, in N. benthamiana protoplasts (B) . The scale bars represent 10 μm.
    Gfp Fluorescence Signal, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Biofunctional effects of EGFR inactivation on different cervical cancer cell lines. ( A ) Dual-colour fluorescence in situ hybridisation (FISH)-validated amplification of the EGFR gene in cervical cancer cell lines. The FISH analysis showing a homogeneously stained region in CaSki cells with gene amplification. ( B ) Western blot indicates the protein level of EGFR in each cell line. ( C ) Each cell line was treated with 10 μ mol l –1 AG1478 to inhibit EGFR function, and cell viability was measured with an MTT assay 98 h later. An equal amount of DMSO was used as a control. * P

    Journal: British Journal of Cancer

    Article Title: EGFR gene amplification is related to adverse clinical outcomes in cervical squamous cell carcinoma, making the EGFR pathway a novel therapeutic target

    doi: 10.1038/bjc.2011.222

    Figure Lengend Snippet: Biofunctional effects of EGFR inactivation on different cervical cancer cell lines. ( A ) Dual-colour fluorescence in situ hybridisation (FISH)-validated amplification of the EGFR gene in cervical cancer cell lines. The FISH analysis showing a homogeneously stained region in CaSki cells with gene amplification. ( B ) Western blot indicates the protein level of EGFR in each cell line. ( C ) Each cell line was treated with 10 μ mol l –1 AG1478 to inhibit EGFR function, and cell viability was measured with an MTT assay 98 h later. An equal amount of DMSO was used as a control. * P

    Article Snippet: Fluorescence in situ hybridisation (FISH) signals were evaluated with an Olympus fluorescence microscope BX41 (Tokyo, Japan) by two individuals who were blind to the treatment history of each patient.

    Techniques: Fluorescence, In Situ, Hybridization, Fluorescence In Situ Hybridization, Amplification, Staining, Western Blot, MTT Assay

    ( A ) Immunoreactivity of EGFR in cervical cancer tissues. Intense immunoreactivity is present in the cytoplasm of cervical squamous carcinoma cells (upper left panel). ( B ) A cervical adenocarcinoma case with negative staining for EGFR (upper right panel). ( C ) Dual-colour fluorescence in situ hybridisation (FISH) demonstrates amplification of the EGFR gene in cervical cancer. FISH analysis showing a homogeneously stained region in a tumour with gene amplification.

    Journal: British Journal of Cancer

    Article Title: EGFR gene amplification is related to adverse clinical outcomes in cervical squamous cell carcinoma, making the EGFR pathway a novel therapeutic target

    doi: 10.1038/bjc.2011.222

    Figure Lengend Snippet: ( A ) Immunoreactivity of EGFR in cervical cancer tissues. Intense immunoreactivity is present in the cytoplasm of cervical squamous carcinoma cells (upper left panel). ( B ) A cervical adenocarcinoma case with negative staining for EGFR (upper right panel). ( C ) Dual-colour fluorescence in situ hybridisation (FISH) demonstrates amplification of the EGFR gene in cervical cancer. FISH analysis showing a homogeneously stained region in a tumour with gene amplification.

    Article Snippet: Fluorescence in situ hybridisation (FISH) signals were evaluated with an Olympus fluorescence microscope BX41 (Tokyo, Japan) by two individuals who were blind to the treatment history of each patient.

    Techniques: Negative Staining, Fluorescence, In Situ, Hybridization, Fluorescence In Situ Hybridization, Amplification, Staining

    Identification of the M.tb proteins interfering autophagy in host cells. ( A ) The PPI network between M.tb and autophagy pathway proteins of host cells. ( B ) Raw264.7 cells expressing GFPph-mCherry-LC3 for autophagy assay was generated using CRISPR/Cas9 system. The stable cell line transfected either with an empty vector or with 38 M.tb genes in the M.tb -autophagy PPI network individually, were treated with rapamycin to induce autophagy. ( C ) Rv2427c and control plasmid were transfected to GFPph-mCherry-LC3 Raw264.7 stable cell line and induce autophagy by rapamycin. Transfection of Rv2427c significantly rescued the rapamycin-induced decrease of GFP/mCherry fluorescence ratio of the aggregation dots. Arrows indicate transfection positive cells. Arrows indicate transfection positive cells. ( D ) Quantification of GFP/mCherry fluorescence ratio of the aggregation dots ( n = 48). ( E ) Verification of the protein-protein interaction by BiFC assay. Rv2427c were co-transfected with WASP and the negative controls into HEK 293T cells, respectively. Cells were harvested 36h after transfection for confocal fluorescence microscopy-based image analysis. The interactions between Rv2427c and prey proteins allow re-formation of a bimolecular fluorescent complex. ( F ) Quantification of the fluorescence.

    Journal: Nucleic Acids Research

    Article Title: Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system

    doi: 10.1093/nar/gkx1173

    Figure Lengend Snippet: Identification of the M.tb proteins interfering autophagy in host cells. ( A ) The PPI network between M.tb and autophagy pathway proteins of host cells. ( B ) Raw264.7 cells expressing GFPph-mCherry-LC3 for autophagy assay was generated using CRISPR/Cas9 system. The stable cell line transfected either with an empty vector or with 38 M.tb genes in the M.tb -autophagy PPI network individually, were treated with rapamycin to induce autophagy. ( C ) Rv2427c and control plasmid were transfected to GFPph-mCherry-LC3 Raw264.7 stable cell line and induce autophagy by rapamycin. Transfection of Rv2427c significantly rescued the rapamycin-induced decrease of GFP/mCherry fluorescence ratio of the aggregation dots. Arrows indicate transfection positive cells. Arrows indicate transfection positive cells. ( D ) Quantification of GFP/mCherry fluorescence ratio of the aggregation dots ( n = 48). ( E ) Verification of the protein-protein interaction by BiFC assay. Rv2427c were co-transfected with WASP and the negative controls into HEK 293T cells, respectively. Cells were harvested 36h after transfection for confocal fluorescence microscopy-based image analysis. The interactions between Rv2427c and prey proteins allow re-formation of a bimolecular fluorescent complex. ( F ) Quantification of the fluorescence.

    Article Snippet: For autophagy assay, the aggregation and intensity of GFP/mCherry fluorescence signal were measured by fluorescence microscopy (Olympus, Japan) and analyzed using ImageJ, as previously described ( ).

    Techniques: Expressing, Generated, CRISPR, Stable Transfection, Transfection, Plasmid Preparation, Fluorescence, Bimolecular Fluorescence Complementation Assay, Microscopy

    tdTomato is expressed in various regions throughout the brain that contain PVALB-expressing neurons “(A) tdTomato expression visualized with an antibody against RFP to enhance signals in relatively dimmer regions (GP, hippocampus, neocortex) for this low magnification montage. Note that tdTomato is expressed throughout the brain including the cortex, globus pallidus, thalamic reticular nucleus, hippocampus and cerebellum and also shows ectopic expression in ependymal cells around the fimbria. The signal appears dim in the striatum due to low number of PVALB-expressing neurons (1–2% of neurons). Scale bar 1 mm. (B) Magnified view of tdTomato expression (red) in PVALB-expressing neurons (green) in the thalamic reticular nucleus. Note the high degree of co-expression (yellow). Scale bars in B–D 100 µm. (C) Magnified view of the globus pallidus. (D) Magnified view of the cerebellum.

    Journal: Neuroscience

    Article Title: Transgenic labeling of parvalbumin-expressing neurons with tdTomato

    doi: 10.1016/j.neuroscience.2015.08.036

    Figure Lengend Snippet: tdTomato is expressed in various regions throughout the brain that contain PVALB-expressing neurons “(A) tdTomato expression visualized with an antibody against RFP to enhance signals in relatively dimmer regions (GP, hippocampus, neocortex) for this low magnification montage. Note that tdTomato is expressed throughout the brain including the cortex, globus pallidus, thalamic reticular nucleus, hippocampus and cerebellum and also shows ectopic expression in ependymal cells around the fimbria. The signal appears dim in the striatum due to low number of PVALB-expressing neurons (1–2% of neurons). Scale bar 1 mm. (B) Magnified view of tdTomato expression (red) in PVALB-expressing neurons (green) in the thalamic reticular nucleus. Note the high degree of co-expression (yellow). Scale bars in B–D 100 µm. (C) Magnified view of the globus pallidus. (D) Magnified view of the cerebellum.

    Article Snippet: Cells were visually identified based on the tdTomato fluorescence signal under a BX-51WI microscope (Olympus) equipped with a mercury arc lamp.

    Techniques: Expressing

    Co-labeling and electrophysiology show that the Pvalb-tdTomato reporter line faithfully labels cortical PVALB-expressing fast-spiking neurons (A) Laser scanning confocal microscopy images show the co-localization of tdTomato (red) and PVALB (green) in cortical neurons. Scale bars 50 µm. Double-labeled cells are shown in yellow. (B) Quantification of the fidelity (fraction of tdTomato- and PVALB-expressing cells of tdTomato-expressing cells) and completeness (fraction of tdTomato- and PVALB-expressing cells of PVALB-expressing cells). (C) Representative trace from a whole-cell patch clamp recording experiment. Several current injection steps are shown. A series of 50 current injection steps were applied with increments of 15 pA starting from −150 pA. Note the suprathreshold fast spiking rate, the subthreshold membrane potential oscillations, and the large amplitude fast after-hyperpolarization, which are typical for PVALB-expressing neurons. (D) linear current-voltage (I–V) plot generated from current injections as shown in C (n=8 cells from 4 mice). The current-firing rate (I-f) plot shows the instantaneous firing frequency upon current injections as shown in C (n=8 cells from 4 mice). Mean ± SEM.

    Journal: Neuroscience

    Article Title: Transgenic labeling of parvalbumin-expressing neurons with tdTomato

    doi: 10.1016/j.neuroscience.2015.08.036

    Figure Lengend Snippet: Co-labeling and electrophysiology show that the Pvalb-tdTomato reporter line faithfully labels cortical PVALB-expressing fast-spiking neurons (A) Laser scanning confocal microscopy images show the co-localization of tdTomato (red) and PVALB (green) in cortical neurons. Scale bars 50 µm. Double-labeled cells are shown in yellow. (B) Quantification of the fidelity (fraction of tdTomato- and PVALB-expressing cells of tdTomato-expressing cells) and completeness (fraction of tdTomato- and PVALB-expressing cells of PVALB-expressing cells). (C) Representative trace from a whole-cell patch clamp recording experiment. Several current injection steps are shown. A series of 50 current injection steps were applied with increments of 15 pA starting from −150 pA. Note the suprathreshold fast spiking rate, the subthreshold membrane potential oscillations, and the large amplitude fast after-hyperpolarization, which are typical for PVALB-expressing neurons. (D) linear current-voltage (I–V) plot generated from current injections as shown in C (n=8 cells from 4 mice). The current-firing rate (I-f) plot shows the instantaneous firing frequency upon current injections as shown in C (n=8 cells from 4 mice). Mean ± SEM.

    Article Snippet: Cells were visually identified based on the tdTomato fluorescence signal under a BX-51WI microscope (Olympus) equipped with a mercury arc lamp.

    Techniques: Labeling, Expressing, Confocal Microscopy, Patch Clamp, Injection, Generated, Mouse Assay

    The Pvalb-tdTomato reporter line reliably labels hippocampal PVALB-expressing neurons (A) Native tdTomato (red) expression and anti-PVALB immunofluorescence labeling (green) is shown. Double-labeled cells are shown in yellow. Scale bars 50 µm. (B) Quantification of the fidelity (fraction of tdTomato- and PVALB-expressing cells of tdTomato-expressing cells) and completeness (fraction of tdTomato- and PVALB-expressing cells of PVALB-expressing cells). (C) Representative trace from a whole-cell patch clamp recording experiment. Several current injection steps are shown. A series of 50 current injection steps were applied with increments of 15 pA starting from −150 pA. Note the suprathreshold fast spiking rate, the subthreshold membrane potential oscillations, and the large amplitude fast after-hyperpolarization, which are typical for PVALB-expressing neurons. (D) I–V plot generated from current injections as shown in C (n=8 cells from 4 mice). I-f plot showing the instantaneous firing frequency upon current injections as shown in C (n=8 cells from 4 mice). Mean ± SEM.

    Journal: Neuroscience

    Article Title: Transgenic labeling of parvalbumin-expressing neurons with tdTomato

    doi: 10.1016/j.neuroscience.2015.08.036

    Figure Lengend Snippet: The Pvalb-tdTomato reporter line reliably labels hippocampal PVALB-expressing neurons (A) Native tdTomato (red) expression and anti-PVALB immunofluorescence labeling (green) is shown. Double-labeled cells are shown in yellow. Scale bars 50 µm. (B) Quantification of the fidelity (fraction of tdTomato- and PVALB-expressing cells of tdTomato-expressing cells) and completeness (fraction of tdTomato- and PVALB-expressing cells of PVALB-expressing cells). (C) Representative trace from a whole-cell patch clamp recording experiment. Several current injection steps are shown. A series of 50 current injection steps were applied with increments of 15 pA starting from −150 pA. Note the suprathreshold fast spiking rate, the subthreshold membrane potential oscillations, and the large amplitude fast after-hyperpolarization, which are typical for PVALB-expressing neurons. (D) I–V plot generated from current injections as shown in C (n=8 cells from 4 mice). I-f plot showing the instantaneous firing frequency upon current injections as shown in C (n=8 cells from 4 mice). Mean ± SEM.

    Article Snippet: Cells were visually identified based on the tdTomato fluorescence signal under a BX-51WI microscope (Olympus) equipped with a mercury arc lamp.

    Techniques: Expressing, Immunofluorescence, Labeling, Patch Clamp, Injection, Generated, Mouse Assay

    Pvalb-tdTomato BAC trimming and recombineering strategy (A) Diagram of the unmodified Pvalb -spanning BAC clone RP24-306A6. The BAC clone also spans the extra gene Ift27 . The positions of two homology arms selected for BAC trimming are shown (green arrows). (B) Diagram of the BAC clone following BAC trimming, which removes the Ift27 gene. Note that BAC trimming steps eliminated the loxP site present in the BAC vector. (C) Diagram of BAC recombineering steps employed to insert the tdTomato cDNA sequence immediately downstream of the putative promoter region of the Pvalb gene. Note the addition of a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) to improve transgene expression.

    Journal: Neuroscience

    Article Title: Transgenic labeling of parvalbumin-expressing neurons with tdTomato

    doi: 10.1016/j.neuroscience.2015.08.036

    Figure Lengend Snippet: Pvalb-tdTomato BAC trimming and recombineering strategy (A) Diagram of the unmodified Pvalb -spanning BAC clone RP24-306A6. The BAC clone also spans the extra gene Ift27 . The positions of two homology arms selected for BAC trimming are shown (green arrows). (B) Diagram of the BAC clone following BAC trimming, which removes the Ift27 gene. Note that BAC trimming steps eliminated the loxP site present in the BAC vector. (C) Diagram of BAC recombineering steps employed to insert the tdTomato cDNA sequence immediately downstream of the putative promoter region of the Pvalb gene. Note the addition of a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) to improve transgene expression.

    Article Snippet: Cells were visually identified based on the tdTomato fluorescence signal under a BX-51WI microscope (Olympus) equipped with a mercury arc lamp.

    Techniques: BAC Assay, Plasmid Preparation, Sequencing, Expressing

    BAK1 and BKK1 robustly suppress the expression of SA biosynthesis-related genes SID2 and EDS5 . Seedlings are grown in dark (D) for 4 days then relocated to long-day condition (L) for the indicated days (4D+0L, 4D+1L, 4D+2L). Seedlings grown in dark for 5 or 6 days (5D+0L or 6D+0L) are analyzed as control. Quantitative RT-PCR assays indicate both SID2 and EDS5 are significantly up-regulated in bak1-4 bkk1-1 compared to those in Col-0 (A) . SID2-GFP and EDS5-YFP are detected to locate to the entire chloroplast and chloroplast surface, respectively, in N. benthamiana protoplasts (B) . The scale bars represent 10 μm.

    Journal: Frontiers in Plant Science

    Article Title: Both Light-Induced SA Accumulation and ETI Mediators Contribute to the Cell Death Regulated by BAK1 and BKK1

    doi: 10.3389/fpls.2017.00622

    Figure Lengend Snippet: BAK1 and BKK1 robustly suppress the expression of SA biosynthesis-related genes SID2 and EDS5 . Seedlings are grown in dark (D) for 4 days then relocated to long-day condition (L) for the indicated days (4D+0L, 4D+1L, 4D+2L). Seedlings grown in dark for 5 or 6 days (5D+0L or 6D+0L) are analyzed as control. Quantitative RT-PCR assays indicate both SID2 and EDS5 are significantly up-regulated in bak1-4 bkk1-1 compared to those in Col-0 (A) . SID2-GFP and EDS5-YFP are detected to locate to the entire chloroplast and chloroplast surface, respectively, in N. benthamiana protoplasts (B) . The scale bars represent 10 μm.

    Article Snippet: YFP or GFP fluorescence signal was observed by Olympus FluoView FV1000 confocal microscope.

    Techniques: Expressing, Quantitative RT-PCR