fluorescence signal intensity  (Thermo Fisher)


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    Thermo Fisher fluorescence signal intensity
    Fluorescence Signal Intensity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence signal intensity/product/Thermo Fisher
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    fluorescence signal intensity - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    ROS Assay:

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: .. ROS Assay To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. Treated MyoD-transduced fibroblasts and non-treated fibroblasts were incubated with CellROX® Deep Red Reagent for 30 min at 37°C and washed with PBS.

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: Paragraph title: ROS Assay ... To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured.

    Functional Assay:

    Article Title: Identification of differentially expressed genes between developing seeds of different soybean cultivars
    Article Snippet: Paragraph title: Functional categorization of differentially expressed probe sets The following are the supplementary data related to this article. Supplementary File 1 Supplementary File 2 Supplementary File 3 Supplementary File 4 Supplementary File 5 Supplementary File 6 ... The distribution of the fluorescence signal intensity across all the probes is shown for each of the 54 Affymetrix arrays used in this study.

    Fluorescence:

    Article Title: Identification of differentially expressed genes between developing seeds of different soybean cultivars
    Article Snippet: .. The distribution of the fluorescence signal intensity across all the probes is shown for each of the 54 Affymetrix arrays used in this study. .. Supplementary File 2 Relative log expression values for each Affymetrix array.

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively. .. For data analysis, Fiji and OriginPro® (OriginLab) were used.

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: .. ROS Assay To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. Treated MyoD-transduced fibroblasts and non-treated fibroblasts were incubated with CellROX® Deep Red Reagent for 30 min at 37°C and washed with PBS.

    Article Title: M11l
    Article Snippet: .. To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane . .. CCCP was added to the cell culture medium at a final concentration of 50 μM during and after dye addition.

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: .. To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. Treated MyoD-transduced fibroblasts and non-treated fibroblasts were incubated with CellROX® Deep Red Reagent for 30 min at 37°C and washed with PBS.

    Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
    Article Snippet: .. Fluorescence signal intensity was measured with a microplate reader (Thermo Fischer) at 485 nm excitation and 535 nm emission. .. To measure the function of function and permeability of BRB, animals were deeply anesthetized (10% chloral hydrate, 300 mg/kg) and injected with Evans blue (EB) dye solution through tail vein (45 mg/kg).

    Software:

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: ROS Assay To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. After that, the signal was measured using the software of BIOREVO BZ-9000.

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. After that, the signal was measured using the software of BIOREVO BZ-9000.

    Chromatin Immunoprecipitation:

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: According to the manual instruction, 40 μL of the reaction mix including 20 μL reaction substrate and 20 μL MMP-9 sample was prepared and loaded into each concentrator chip of the ISAAC-12 platform. .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Picogreen Assay:

    Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
    Article Snippet: We used the Quant-iT PicoGreen dsDNA assay kit (Life Technologies, P7589) to measure extracellular DNA to evaluate NETs formation. .. Fluorescence signal intensity was measured with a microplate reader (Thermo Fischer) at 485 nm excitation and 535 nm emission.

    Negative Control:

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: Three negative samples were prepared to eliminate possible assay error: negative control (assay buffer); negative control+1X medium (fresh culture medium); negative control+ 0.1X medium (10-fold dilution of fresh medium with assay buffer). .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Construct:

    Article Title: M11l
    Article Snippet: Paragraph title: Measurement of Mitochondrial Membrane Potential in Cells Expressing M11L Constructs. ... To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane .

    Cytometry:

    Article Title: M11l
    Article Snippet: To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane . .. Flow cytometric analysis was conducted using a Becton Dickinson FACSCalibur™ flow cytometer equipped with an argon ion laser with 15 mW of excitation at 488 nm.

    Concentration Assay:

    Article Title: M11l
    Article Snippet: To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane . .. CCCP was added to the cell culture medium at a final concentration of 50 μM during and after dye addition.

    Incubation:

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: An external DC voltage of 10 V was applied across all the microchannels to start ICP and the fluorescence signal intensity of reaction product 5-FAM was monitored in each well over 8 min. For comparison, standard assays under static incubation of the reaction mix were also performed both in an array of microchannels without ICP as well as in a 384-well plate at room temperature. .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: ROS Assay To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. Treated MyoD-transduced fibroblasts and non-treated fibroblasts were incubated with CellROX® Deep Red Reagent for 30 min at 37°C and washed with PBS.

    Article Title: M11l
    Article Snippet: Cells were incubated with 0.5 nM DiOC6 at 37°C for 10 min, harvested with trypsin, and then washed and resuspended in RPMI lacking phenol red (GIBCO BRL). .. To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane .

    Article Title: Efficacy of Prednisolone in Generated Myotubes Derived From Fibroblasts of Duchenne Muscular Dystrophy Patients
    Article Snippet: To measure cellular ROS production, the fluorescence signal intensity of CellROX® Deep Red Reagent (Thermo Fisher Scientific, Rockford, IL, United States) was measured. .. Treated MyoD-transduced fibroblasts and non-treated fibroblasts were incubated with CellROX® Deep Red Reagent for 30 min at 37°C and washed with PBS.

    Cell Culture:

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: Two positive samples were prepared to measure MMP-9 expression level: 1X MMP-9 cell culture supernatant sample (without dilution) and 0.1X MMP-9 cell culture supernatant sample (10-fold dilution with assay buffer). .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Article Title: M11l
    Article Snippet: Rat2puro or Rat2M11L cells were cultured in 12-well plates (5 × 105 cells/well) and treated with 2 μM staurosporine for 4 h. As the staurosporine stock was prepared in DMSO, control cells were treated with an equivalent amount of DMSO alone. .. To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane .

    Expressing:

    Article Title: Identification of differentially expressed genes between developing seeds of different soybean cultivars
    Article Snippet: The distribution of the fluorescence signal intensity across all the probes is shown for each of the 54 Affymetrix arrays used in this study. .. Supplementary File 2 Relative log expression values for each Affymetrix array.

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: Two positive samples were prepared to measure MMP-9 expression level: 1X MMP-9 cell culture supernatant sample (without dilution) and 0.1X MMP-9 cell culture supernatant sample (10-fold dilution with assay buffer). .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Article Title: M11l
    Article Snippet: Paragraph title: Measurement of Mitochondrial Membrane Potential in Cells Expressing M11L Constructs. ... To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane .

    Staining:

    Article Title: M11l
    Article Snippet: Thereafter, the cells were stained with the green fluorescent dye 3,3′dihexyloxacarbocyanine iodide (DiOC6 ; Molecular Probes). .. To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane .

    Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
    Article Snippet: Pico-Green is a fluorescent dye binding to extracellular dsDNA without staining live cells. .. Fluorescence signal intensity was measured with a microplate reader (Thermo Fischer) at 485 nm excitation and 535 nm emission.

    Binding Assay:

    Article Title: Hyperglycemia Induces Neutrophil Extracellular Traps Formation Through an NADPH Oxidase-Dependent Pathway in Diabetic Retinopathy
    Article Snippet: Pico-Green is a fluorescent dye binding to extracellular dsDNA without staining live cells. .. Fluorescence signal intensity was measured with a microplate reader (Thermo Fischer) at 485 nm excitation and 535 nm emission.

    Dilution Assay:

    Article Title: A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays
    Article Snippet: Two positive samples were prepared to measure MMP-9 expression level: 1X MMP-9 cell culture supernatant sample (without dilution) and 0.1X MMP-9 cell culture supernatant sample (10-fold dilution with assay buffer). .. The fluorescence signal intensity of reaction product in the microchannels and in the 384-well plate was measured with an EMCCD camera (ANDOR, iXon EMCCD 897) and Varioskan® Flash plate reader (Thermo Scientific), respectively.

    Flow Cytometry:

    Article Title: M11l
    Article Snippet: To verify that a decrease in the fluorescence signal intensity did accompany a loss of mitochondrial membrane potential, control cells were treated with the protonophore carbonyl cyanide m -chlorophenyl hydrazone (CCCP; Molecular Probes), which causes dissipation of the proton gradient across the mitochondria inner membrane . .. Flow cytometric analysis was conducted using a Becton Dickinson FACSCalibur™ flow cytometer equipped with an argon ion laser with 15 mW of excitation at 488 nm.

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  • News
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  • Contact
  • Bioz Stars
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  • 94
    Thermo Fisher fluorescence signal intensity
    Fluorescence Signal Intensity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence signal intensity/product/Thermo Fisher
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    fluorescence signal intensity - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    96
    Thermo Fisher fluorescent signal intensity
    Fluorescent Signal Intensity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent signal intensity/product/Thermo Fisher
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fluorescent signal intensity - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

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