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BioTek Instruments fluorescence signal intensity
Fluorescence Signal Intensity, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence signal intensity/product/BioTek Instruments
Average 97 stars, based on 3 article reviews
Price from $9.99 to $1999.99
fluorescence signal intensity - by Bioz Stars, 2020-03
97/100 stars

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Related Articles

Microscopy:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: The cells were then analyzed by fluorescence microscopy to detect GFP gene expression. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions. .. Images of the central scratch and surrounding area were captured every 8 minutes for 24 hours using the Nikon-Eclipse TI-U inverted microscope (Nikon, Melville, NY) and MetaMorph ® Microscopy Automation & Image Analysis Software (Molecular Devices, Sunnyvale, CA, USA) and compiled into a movie.

Imaging:

Article Title: Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport
Article Snippet: The cells were washed (pelleted and resuspended) twice with water and finally suspended in imaging buffer (10 mM Tris-HCl, pH 7.4, and 2% glucose [wt/vol]). .. The cells were pelleted, washed once with water, and transferred to a 96-well plate at a final concentration of 0.01 OD600 /ml, and the fluorescence signal intensity was measured using excitation at 360/40 and emission at 460/40 with a multimode plate reader (Synergy HT; BioTek).

Transfection:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: A cytopathic effect was observed after 10 days, and the transfected cells became round and detached from the plate. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Amplification:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: An individual plaque of the adenovirus vector that encoded and expressed the GFP gene (Ad-GFP) was amplified. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Expressing:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: The cells were then analyzed by fluorescence microscopy to detect GFP gene expression. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Fluorescence:

Article Title: Tear Fluid Extracellular DNA: Diagnostic and Therapeutic Implications in Dry Eye Disease
Article Snippet: .. Fluorescence signal intensity was measured (excitation at 480 nm and emission at 520 nm) using a microplate reader (Synergy H1; BioTek, Winooski, VT). .. After 5-minute incubation, fluorescence signal intensity was measured every 15 seconds over 20 minutes.

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter. ..

Article Title: Beta-Hydroxybutyrate Promotes Proliferation, Migration and Stemness in a Subpopulation of 5FU Treated SW480 Cells: Evidence for Metabolic Plasticity in Colon Cancer
Article Snippet: .. For OCR measurement as recommendations by manufacturer, after addition of the probe and 2 drop of mineral oil for sealing, Fluorescence signal intensity was measured kinetically on a plate reader (Cytation 3, Bio-Tek) at 37°C every 1:30 minutes for at least 120 minutes in time-resolved fluorescence (TR-F) mode using a 365 nm excitation and 650 nm emission filters, delay times 30 and 70μs. ..

Article Title: A High-Performance Fluorescence Immunoassay Based on the Relaxation of Quenching, Exemplified by Detection of Cardiac Troponin I
Article Snippet: .. Red BSA conjugated with SA diluted in BSA-PBS to 0–4.35 g/mL was added to the treated microwells, followed by incubation at 37 °C for 1 h. The fluorescence signal intensity was measured at an excitation wavelength of 590 nm and emission wavelength of 620 nm using a microplate reader (Synergy™ H4, BioTek Inc.; Winooski, VT, USA). ..

Article Title: Hyperosmolar Stress Induces Neutrophil Extracellular Trap Formation: Implications for Dry Eye Disease
Article Snippet: .. Fluorescence signal intensity was measured at excitation, 480 nm, and emission, 520 nm, using a microplate reader (Synergy H1; BioTek, Winooski, VT, USA). ..

Article Title: Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport
Article Snippet: .. The cells were pelleted, washed once with water, and transferred to a 96-well plate at a final concentration of 0.01 OD600 /ml, and the fluorescence signal intensity was measured using excitation at 360/40 and emission at 460/40 with a multimode plate reader (Synergy HT; BioTek). ..

Article Title: Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling
Article Snippet: .. The fluorescence signal intensity of the bottom chamber was then measured after 1 hour of incubation using a plate reader (Synergy H1, BioTek) with excitation and emission wavelengths of 485 nm and 530 nm, respectively. ..

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: .. Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions. .. For scratch wound healing assays, EPC2-hTERT cells were seeded in 24-well plates.

FITC-Dextran Permeability Assay:

Article Title: Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling
Article Snippet: Paragraph title: 2.6 Fluorescein isothiocyanate (FITC) –Dextran Permeability Assay ... The fluorescence signal intensity of the bottom chamber was then measured after 1 hour of incubation using a plate reader (Synergy H1, BioTek) with excitation and emission wavelengths of 485 nm and 530 nm, respectively.

Live Cell Imaging:

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: Cell migration was assessed by ORIS™ cell migration assays (Platypus Technologies, Madison, WI, USA) and scratch wound healing assays coupled with live cell imaging. .. Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions.

Picogreen Assay:

Article Title: Tear Fluid Extracellular DNA: Diagnostic and Therapeutic Implications in Dry Eye Disease
Article Snippet: The PicoGreen dye fluorescence assay was performed using the Quant-iT PicoGreen dsDNA Assay Kit (catalog number P7589; Invitrogen, Carlsbad, CA) per the manufacturer's instructions. .. Fluorescence signal intensity was measured (excitation at 480 nm and emission at 520 nm) using a microplate reader (Synergy H1; BioTek, Winooski, VT).

Article Title: Hyperosmolar Stress Induces Neutrophil Extracellular Trap Formation: Implications for Dry Eye Disease
Article Snippet: Paragraph title: PicoGreen Assay for Measurement of eDNA ... Fluorescence signal intensity was measured at excitation, 480 nm, and emission, 520 nm, using a microplate reader (Synergy H1; BioTek, Winooski, VT, USA).

Labeling:

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: .. Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions. .. For scratch wound healing assays, EPC2-hTERT cells were seeded in 24-well plates.

Purification:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: The 750-bp fragment was purified from the gel using a Qiagen kit and was subcloned into the NotI site of the adenoviral shuttle plasmid between the cytomegalovirus (CMV) immediate-early promoter and the polyadenylation signal from bovine growth hormone. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Produced:

Article Title: Hyperosmolar Stress Induces Neutrophil Extracellular Trap Formation: Implications for Dry Eye Disease
Article Snippet: The Quant-iT PicoGreen dsDNA assay kit (cat. no. P7589; Life Technologies) was used to quantify eDNA as a measure of the amount of NETs produced, as previously described. .. Fluorescence signal intensity was measured at excitation, 480 nm, and emission, 520 nm, using a microplate reader (Synergy H1; BioTek, Winooski, VT, USA).

Concentration Assay:

Article Title: Beta-Hydroxybutyrate Promotes Proliferation, Migration and Stemness in a Subpopulation of 5FU Treated SW480 Cells: Evidence for Metabolic Plasticity in Colon Cancer
Article Snippet: Briefly, about 70,000 cells/well in 96-well plates were cultured overnight in a standard CO2 incubator and then cells were treated with BHB (at the concentration equal to calculated EC50 dose at 250µM) for 24 h that maintained for the duration of the experiment. .. For OCR measurement as recommendations by manufacturer, after addition of the probe and 2 drop of mineral oil for sealing, Fluorescence signal intensity was measured kinetically on a plate reader (Cytation 3, Bio-Tek) at 37°C every 1:30 minutes for at least 120 minutes in time-resolved fluorescence (TR-F) mode using a 365 nm excitation and 650 nm emission filters, delay times 30 and 70μs.

Article Title: Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport
Article Snippet: .. The cells were pelleted, washed once with water, and transferred to a 96-well plate at a final concentration of 0.01 OD600 /ml, and the fluorescence signal intensity was measured using excitation at 360/40 and emission at 460/40 with a multimode plate reader (Synergy HT; BioTek). ..

Incubation:

Article Title: Tear Fluid Extracellular DNA: Diagnostic and Therapeutic Implications in Dry Eye Disease
Article Snippet: Fluorescence signal intensity was measured (excitation at 480 nm and emission at 520 nm) using a microplate reader (Synergy H1; BioTek, Winooski, VT). .. After 5-minute incubation, fluorescence signal intensity was measured every 15 seconds over 20 minutes.

Article Title: A High-Performance Fluorescence Immunoassay Based on the Relaxation of Quenching, Exemplified by Detection of Cardiac Troponin I
Article Snippet: .. Red BSA conjugated with SA diluted in BSA-PBS to 0–4.35 g/mL was added to the treated microwells, followed by incubation at 37 °C for 1 h. The fluorescence signal intensity was measured at an excitation wavelength of 590 nm and emission wavelength of 620 nm using a microplate reader (Synergy™ H4, BioTek Inc.; Winooski, VT, USA). ..

Article Title: Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling
Article Snippet: .. The fluorescence signal intensity of the bottom chamber was then measured after 1 hour of incubation using a plate reader (Synergy H1, BioTek) with excitation and emission wavelengths of 485 nm and 530 nm, respectively. ..

Cell Culture:

Article Title: Beta-Hydroxybutyrate Promotes Proliferation, Migration and Stemness in a Subpopulation of 5FU Treated SW480 Cells: Evidence for Metabolic Plasticity in Colon Cancer
Article Snippet: Briefly, about 70,000 cells/well in 96-well plates were cultured overnight in a standard CO2 incubator and then cells were treated with BHB (at the concentration equal to calculated EC50 dose at 250µM) for 24 h that maintained for the duration of the experiment. .. For OCR measurement as recommendations by manufacturer, after addition of the probe and 2 drop of mineral oil for sealing, Fluorescence signal intensity was measured kinetically on a plate reader (Cytation 3, Bio-Tek) at 37°C every 1:30 minutes for at least 120 minutes in time-resolved fluorescence (TR-F) mode using a 365 nm excitation and 650 nm emission filters, delay times 30 and 70μs.

Article Title: Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling
Article Snippet: HUVEC were seeded onto collagen-coated Transwell® (3413, Corning) inserts at 100,000 cells/cm2 and cultured until confluent, then exposed to TiO2 , SiO2 , and PS NPs at the concentrations that yield the same total surface area in EGM-2 for 2 hours, the apical chamber solution was replaced with 1 mg/mL 70-kD FITC-Dextran (46945, Sigma) in PBS supplemented with Ca2+ and Mg2+ , and the bottom chamber solution was replaced with PBS. .. The fluorescence signal intensity of the bottom chamber was then measured after 1 hour of incubation using a plate reader (Synergy H1, BioTek) with excitation and emission wavelengths of 485 nm and 530 nm, respectively.

Migration:

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: Paragraph title: Migration assays ... Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions.

Inverted Microscopy:

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions. .. Images of the central scratch and surrounding area were captured every 8 minutes for 24 hours using the Nikon-Eclipse TI-U inverted microscope (Nikon, Melville, NY) and MetaMorph ® Microscopy Automation & Image Analysis Software (Molecular Devices, Sunnyvale, CA, USA) and compiled into a movie.

Sequencing:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: The plasmid pJM17 containing the full-length of the adenovirus genome including a 4.4-kb sequence of antibiotic-resistant gene, was co-transfected in HEK293 cells along with the shuttle plasmid containing the GFP gene flanked by the adenovirus E1 sequences. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Staining:

Article Title: Hyperosmolar Stress Induces Neutrophil Extracellular Trap Formation: Implications for Dry Eye Disease
Article Snippet: PicoGreen is a cell-impermeant dye that binds to extracellular double stranded Deoxyribonucleic acid (dsDNA) without staining live cells. .. Fluorescence signal intensity was measured at excitation, 480 nm, and emission, 520 nm, using a microplate reader (Synergy H1; BioTek, Winooski, VT, USA).

Article Title: Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport
Article Snippet: Paragraph title: CW staining ... The cells were pelleted, washed once with water, and transferred to a 96-well plate at a final concentration of 0.01 OD600 /ml, and the fluorescence signal intensity was measured using excitation at 360/40 and emission at 460/40 with a multimode plate reader (Synergy HT; BioTek).

Plasmid Preparation:

Article Title: Milk Inhibits the Biological Activity of Ricin
Article Snippet: An individual plaque of the adenovirus vector that encoded and expressed the GFP gene (Ad-GFP) was amplified. .. The presence of GFP was confirmed by measuring the fluorescence signal intensity in transduced cells in a Synergy HT Multi-Detection Microplate Reader (BioTek, Winooki, VT) with a 485-nm excitation wavelength using a 485/20 excitation filter and a 528-nm emission wavelength using a 528/20 emission filter.

Software:

Article Title: Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition
Article Snippet: Migrated cells were then labeled with a Calcein fluorescent dye (Sigma, St. Louis, MO) and subjected to quantification of a fluorescence signal intensity using a multi-mode microplate reader (Biotek HT Synergy, Winooski, VT) according to manufacturer’s instructions. .. Images of the central scratch and surrounding area were captured every 8 minutes for 24 hours using the Nikon-Eclipse TI-U inverted microscope (Nikon, Melville, NY) and MetaMorph ® Microscopy Automation & Image Analysis Software (Molecular Devices, Sunnyvale, CA, USA) and compiled into a movie.

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    BioTek Instruments fluorescence signal intensity
    Fluorescence Signal Intensity, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence signal intensity/product/BioTek Instruments
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fluorescence signal intensity - by Bioz Stars, 2020-03
    97/100 stars
      Buy from Supplier

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