fluoresceine conjugated rca 1  (Vector Laboratories)

Journal: Frontiers in Pharmacology

Article Title: Imipramine and Venlafaxine Differentially Affect Primary Glial Cultures of Prenatally Stressed Rats

doi: 10.3389/fphar.2019.01687

Figure Lengend Snippet: Effects of prenatal stress and administration of imipramine (IMI) or venlafaxine (VEN) during pregnancy on morphology of mixed glial and a microglia culture. IMI and VEN were administered via oral gavage. Representative images of mixed glial culture on day 8 in the bright field (using cell analyzer JuLI, magnification 20×) are shown for (A) control group, (D) prenatal stress group, (G) stress+IMI group, and (J) stress+VEN group. Representative phase contrast images were taken on microglia separated from the mixed glial culture on day 13. Cellular nuclei were stained using Hoechst 33342 (blue), using a fluorescent microscope (Nikon TS-100F, magnification 40×): (B) control group, (E) prenatal stress group, (H) stress+IMI group, and (K) stress+VEN group. Microglial cells were stained with fluorescein conjugated agglutinin-1 ( R. communis ), which is a marker of surface glycoproteins on microglia (green), using a fluorescent microscope (Nikon TS-100F, magnification 40×): (C) control group, (F) prenatal stress group, (I) stress+IMI group, and (L) stress+VEN group. For interpretation of the references to color in this figure legend, please see the web version of this article.

Article Snippet: Next, cells were incubated overnight at 4°C with fluorescein conjugated Ricinus communis agglutinin-1 (1:200) (FL-1081, Vector, USA), which is a lectin that binds to the surface glycoproteins on microglia.

Techniques: Staining, Microscopy, Marker