fluorescein rabbit antirat  (Vector Laboratories)


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Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
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    Name:
    Fluorescein Rabbit Anti Rat IgG Antibody
    Description:
    Rabbit Anti Rat IgG Antibody is prepared by hyperimmunizing animals in a manner that produces high affinity antibodies These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present Cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The final product is then subjected to rigorous quality control assays including immunodiffusion solid phase enzyme immunoassays gel electrophoresis and solid phase binding assays In preparing the labeled antibodies great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody The labeled antibody then undergoes a further series of quality control assays including immunohistochemical analysis Unless otherwise specified our antibodies will recognize both heavy and light chains H L Fluorescein Rabbit Anti Rat IgG H L Antibody can be used for flow cytometry or for tissue staining Optimal F P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining Maximum excitation is at 495 nm and maximum emission is at 515 nm
    Catalog Number:
    fi-4000
    Price:
    None
    Host:
    Rabbit
    Size:
    1 5 mg
    Category:
    Antibodies
    Reactivity:
    Rat
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    Structured Review

    Vector Laboratories fluorescein rabbit antirat
    Fluorescein Rabbit Anti Rat IgG Antibody
    Rabbit Anti Rat IgG Antibody is prepared by hyperimmunizing animals in a manner that produces high affinity antibodies These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present Cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The final product is then subjected to rigorous quality control assays including immunodiffusion solid phase enzyme immunoassays gel electrophoresis and solid phase binding assays In preparing the labeled antibodies great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody The labeled antibody then undergoes a further series of quality control assays including immunohistochemical analysis Unless otherwise specified our antibodies will recognize both heavy and light chains H L Fluorescein Rabbit Anti Rat IgG H L Antibody can be used for flow cytometry or for tissue staining Optimal F P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining Maximum excitation is at 495 nm and maximum emission is at 515 nm
    https://www.bioz.com/result/fluorescein rabbit antirat/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein rabbit antirat - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Marker:

    Article Title: Roles of Gr-1+ Leukocytes in Postincisional Nociceptive Sensitization and Inflammation
    Article Snippet: .. Briefly, the primary and secondary antibodies used were IL-1β(H-153 )Rabbit Polyclonal IgG 1:50 (Santa Cruz Biotechnology, Santa Cruz, CA) and Fluoresecein Anti-rabbit IgG(H+L) 1:150 (Jackson ImmunoResearch Laboratories, West Grove, PA), Rat Anti-mouse Neutrophil (Allotypic Marker) Monoclonal Antibody 1:300 (AbD Serotec, Raleigh, NC ) and Texas Red Anti-rat IgG(H+L) 1:150 (Vector Lab Inc., Burlingame, CA), Rat Anti-mouse F4/80 Antigen 1:100 (AbD Serotec) and Fluorescein Anti-rat IgG(H+L) 1:500 (Vector Lab Inc.), Rabbit anti-β-endorphin 1:100 (Peninsula Laboratories LLC, San Carlos, CA) and Texas Red Anti-rabbit IgG(H+L) 1:500 (Jackson ImmunoResearch Laboratories). .. Confocal laser-scanning microscopy was performed using Zeiss LSM 510 and LSM 510 META Laser Scanning Microscopes (Thornwood, NY).

    Incubation:

    Article Title: Effects of the Histone Deacetylase Inhibitor Valproic Acid on Human Pericytes In Vitro
    Article Snippet: .. Cells were labeled in PBS containing 0.2% BSA for 20 minutes on ice, washed twice with PBS containing 0.2% BSA before a 20 minute incubation on ice with the secondary antibody diluted in PBS containing 0.2% BSA (Allophycocyanin-goat anti mouse IgG (115-136-072, Jackson Immuno Research, UK), FITC-goat anti mouse IgM (115-096-075, Jackson Immuno Research) or FITC-rat anti mouse IgG (FI-4000, Vector Laboratories Inc, CA, USA). .. Cells were then washed two times before being analyzed by flow cytometry on a FACSAria flow cytometer (BD Biosciences).

    Article Title: Pathology of Interstitial Cells of Cajal in Relation to Inflammation Revealed by Ultrastructure But Not Immunohistochemistry
    Article Snippet: .. After the incubation with primary antibody for 2 nights at 4°C, fluorescein isothiocyanate-coupled rabbit anti-rat IgG (Vector Laboratories, Burlingame, CA) was added to the tissue with a dilution of 1:100 and left for 1 hour. .. Tissues were examined with a confocal microscope (LSM 510; Zeiss, Germany) with an excitation wavelength appropriate for fluorescein isothiocyanate (494 nm).

    Article Title: Cholinergic Excitation of Septohippocampal GABA But Not Cholinergic Neurons: Implications for Learning and Memory
    Article Snippet: .. Subsequently, sections were incubated in rabbit anti-rat IgG-fluorescein-labeled (1:100 in PB; Vector Laboratories, Burlingame, CA; FI4000) for 2 hr at room temperature in the dark. .. For Parv, the sections were incubated in a rabbit anti-Parv (1:500 dilution in PB; gift of K. G. Baimbridge, Vancouver, Canada; overnight at room temperature) followed by goat anti-rabbit IgG-fluorescein (1:100 in PB; Vector Laboratories; FI1000; 2 hr at room temperature in the dark).

    Article Title: Cellular Origins of Regenerating Nodules and Malignancy in the FAH Model of Liver Injury after Bone Marrow Cell Transplantation
    Article Snippet: .. Sections were incubated with appropriate secondary antibodies including Alexa 594 goat anti-rabbit or chicken IgG (H+L), a FITC-coupled goat anti-chicken antibody (ICL Lab), Alexa Fluor 488 donkey anti-rabbit IgG (H+L) (Molecular Probes, Eugene, OR), or fluorescein rabbit anti-rat IgG (H+L) (Vector Laboratories). .. After rinsing with PBS, the sections were mounted with 4,6-diaminidino-2-phenylindole (DAPI) and microscopy was performed using a fluorescence microscope (DM IRE2 or DM IRB, Leica, Germany) equipped with a triple bandpass filter to detect emissions at 520 nm for FITC, Alexa Fluor 488, or fluorescein, 610 nm for Alexa 594, and 420 nm for DAPI.

    Labeling:

    Article Title: Effects of the Histone Deacetylase Inhibitor Valproic Acid on Human Pericytes In Vitro
    Article Snippet: .. Cells were labeled in PBS containing 0.2% BSA for 20 minutes on ice, washed twice with PBS containing 0.2% BSA before a 20 minute incubation on ice with the secondary antibody diluted in PBS containing 0.2% BSA (Allophycocyanin-goat anti mouse IgG (115-136-072, Jackson Immuno Research, UK), FITC-goat anti mouse IgM (115-096-075, Jackson Immuno Research) or FITC-rat anti mouse IgG (FI-4000, Vector Laboratories Inc, CA, USA). .. Cells were then washed two times before being analyzed by flow cytometry on a FACSAria flow cytometer (BD Biosciences).

    Article Title: Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation
    Article Snippet: .. The secondary antibody used for indirect labeling was R-Phycoerythrin F(ab’)2 Fragment donkey anti-goat IgG (Jackson ImmunoResearch Inc.) and Fluorescein Rabbit Anti-Rat IgG (Vector Laboratories). .. Cells were analyzed with a Beckman-Coulter Cytomics FC500 flow cytometer.

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  • 93
    Vector Laboratories fluorescein rabbit antirat
    Fluorescein Rabbit Antirat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein rabbit antirat/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein rabbit antirat - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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