fluorescein 12 dutp  (Thermo Fisher)


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    Thermo Fisher fluorescein 12 dutp
    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with <t>fluorescein-12-dUTP</t> (green).
    Fluorescein 12 Dutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp/product/Thermo Fisher
    Average 94 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines"

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34545-x

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).
    Figure Legend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Techniques Used: In Situ Hybridization

    2) Product Images from "XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima"

    Article Title: XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0150292

    The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.
    Figure Legend Snippet: The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.

    Techniques Used: Staining, Construct, Hybridization, Derivative Assay

    3) Product Images from "Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines"

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34545-x

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).
    Figure Legend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Techniques Used: In Situ Hybridization

    4) Product Images from "Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies"

    Article Title: Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies

    Journal: BMC Evolutionary Biology

    doi: 10.1186/s12862-015-0375-4

    Genomic in situ hybridization (GISH) in pachytene oocytes of Leptidea juvernica ( a–d ), L. sinapis ( e–h ) and L. reali ( i–l ). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green) and chromosomes were counterstained with DAPI (blue). Figures ( a – d ), ( e – h ) and ( i – l ) show detailed analyses of sex chromosome multivalents W 1-n Z 1-n : ( a , e , i ) merged images of female genomic probes and DAPI staining; ( b , f , j ) DAPI images; arrows indicate DAPI-positive W-chromosome segments and heterochromatic blocks at the end of the W chromosomes; ( c , g , k ) hybridization pattern of the female genomic probes; the asterisk indicates an undifferentiated segment of one of the W chromosomes; ( d , h , l ) schematic drawings of the sex chromosome multivalents. Scale bar = 10 μm
    Figure Legend Snippet: Genomic in situ hybridization (GISH) in pachytene oocytes of Leptidea juvernica ( a–d ), L. sinapis ( e–h ) and L. reali ( i–l ). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green) and chromosomes were counterstained with DAPI (blue). Figures ( a – d ), ( e – h ) and ( i – l ) show detailed analyses of sex chromosome multivalents W 1-n Z 1-n : ( a , e , i ) merged images of female genomic probes and DAPI staining; ( b , f , j ) DAPI images; arrows indicate DAPI-positive W-chromosome segments and heterochromatic blocks at the end of the W chromosomes; ( c , g , k ) hybridization pattern of the female genomic probes; the asterisk indicates an undifferentiated segment of one of the W chromosomes; ( d , h , l ) schematic drawings of the sex chromosome multivalents. Scale bar = 10 μm

    Techniques Used: In Situ Hybridization, Derivative Assay, Staining, Hybridization

    Comparative genomic hybridization (CGH) in pachytene oocytes of Leptidea juvernica ( a–e ), L. sinapis ( f–j ) and L. reali ( k–o ). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green), male-derived genomic probes were labelled with Cy3-dUTP (red) and chromosomes were counterstained with DAPI (blue). Figures ( a – e ), ( f – j ) and ( k – o ) show detailed analyses of sex chromosome multivalents W 1-n Z 1-n : ( a , f , k ) merged images of both genomic probes and DAPI staining; ( b , g , l ) DAPI images; ( c , h , m ) female genomic probes; arrows indicate W-chromosome segments with female-specific hybridization pattern; ( d , i , n ) male genomic probes; ( e , j , o ) schematic drawings of the sex chromosome multivalents. Scale bars = 10 μm
    Figure Legend Snippet: Comparative genomic hybridization (CGH) in pachytene oocytes of Leptidea juvernica ( a–e ), L. sinapis ( f–j ) and L. reali ( k–o ). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green), male-derived genomic probes were labelled with Cy3-dUTP (red) and chromosomes were counterstained with DAPI (blue). Figures ( a – e ), ( f – j ) and ( k – o ) show detailed analyses of sex chromosome multivalents W 1-n Z 1-n : ( a , f , k ) merged images of both genomic probes and DAPI staining; ( b , g , l ) DAPI images; ( c , h , m ) female genomic probes; arrows indicate W-chromosome segments with female-specific hybridization pattern; ( d , i , n ) male genomic probes; ( e , j , o ) schematic drawings of the sex chromosome multivalents. Scale bars = 10 μm

    Techniques Used: Hybridization, Derivative Assay, Staining

    5) Product Images from "Chromosomal Evolution in Tortricid Moths: Conserved Karyotypes with Diverged Features"

    Article Title: Chromosomal Evolution in Tortricid Moths: Conserved Karyotypes with Diverged Features

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064520

    Comparative genomic hybridization (CGH) in pachytene oocytes of Cydia pomonella (a-e), Grapholita molesta (f-j), Lobesia botrana (k-o), and Eupoecilia ambiguella (p-t). Chromosomes were counterstained with DAPI (blue); female-derived genomic probes were labeled with fluorescein-12-dUTP (green), male-derived genomic probes with Cy3-dUTP (red). Figures a–e , f–j , k–o , and p–t show a detailed analysis of individual WZ bivalents: a , f , k , p – merged image of both probes including counterstaining; b , g , l , q – schematic interpretation of WZ bivalents; c , h , m , r – DAPI image; d , i , n , s – male genomic probe; e , j, o , t – female genomic probe. Arrows indicate WZ bivalents. Bar = 10 µm; a–o and p–t have the same scale.
    Figure Legend Snippet: Comparative genomic hybridization (CGH) in pachytene oocytes of Cydia pomonella (a-e), Grapholita molesta (f-j), Lobesia botrana (k-o), and Eupoecilia ambiguella (p-t). Chromosomes were counterstained with DAPI (blue); female-derived genomic probes were labeled with fluorescein-12-dUTP (green), male-derived genomic probes with Cy3-dUTP (red). Figures a–e , f–j , k–o , and p–t show a detailed analysis of individual WZ bivalents: a , f , k , p – merged image of both probes including counterstaining; b , g , l , q – schematic interpretation of WZ bivalents; c , h , m , r – DAPI image; d , i , n , s – male genomic probe; e , j, o , t – female genomic probe. Arrows indicate WZ bivalents. Bar = 10 µm; a–o and p–t have the same scale.

    Techniques Used: Hybridization, Derivative Assay, Labeling

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    Thermo Fisher fluorescein 12 dutp solution
    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of <t>fluorescein-12-dUTP</t> during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.
    Fluorescein 12 Dutp Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp solution/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp solution - by Bioz Stars, 2022-10
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher fluorescein 12 dutp
    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with <t>fluorescein-12-dUTP</t> (green).
    Fluorescein 12 Dutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.

    Journal: Bio-protocol

    Article Title: TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions

    doi: 10.21769/BioProtoc.2502

    Figure Lengend Snippet: Micrographs showing salt stress-treated rice root tissue subjected to TUNEL assay to assess in situ DNA fragmentation. Root tissue from 15-day old rice seedlings was subjected to salinity stress (200 mM NaCl) for 60 h followed by TUNEL assay as per the protocol described above. F: Fluorescein (green), PI: Propidium Iodide (red), DAPI: 4’,6-diamidino-2-phenylindole (blue). Samples were mounted on slides in a mountant (ProLong ® Gold Antifade with DAPI, Thermo Fisher Scientific) and the slides were examined under Nikon A1R (Nikon, Japan) confocal microscope using a 20x objective. NIS Elements AR software (Nikon, Japan) was used to acquire and process the images. DAPI and PI were used to stain DNA (both damaged and undamaged). Fluorescein fluorescence (green) is due to incorporation of fluorescein-12-dUTP during the TUNEL reaction and would correspond to the number of free DNA ends. Scale bars = 100 µm.

    Article Snippet: Fluorescein-12-dUTP* (Thermo Fisher Scientific, Thermo ScientificTM, catalog number: R0101). dATP* (Promega, catalog number: U1202).

    Techniques: TUNEL Assay, In Situ, Microscopy, Software, Staining, Fluorescence

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Journal: Scientific Reports

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    doi: 10.1038/s41598-018-34545-x

    Figure Lengend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Article Snippet: L. mollis genomic DNA was labeled with fluorescein-12-dUTP (Thermo Scientific) using Random Primers DNA Labeling System (Invitrogen).

    Techniques: In Situ Hybridization

    The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.

    Journal: PLoS ONE

    Article Title: XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima

    doi: 10.1371/journal.pone.0150292

    Figure Lengend Snippet: The karyotype of Strigamia maritima . (A) Inverted image of a DAPI-stained mitotic metaphase prepared from embryonic material, of which the sex was unknown (left panel). The constructed karyotype (right panel) is a representative example of karyotypes prepared from at least 10 other embryos. It shows a large pair of metacentric chromosomes and seven middle to small acrocentric pairs, gradually decreasing in size. All karyotypes give qualitatively the same result and none of them show evidence for a heteromorphic sex chromosome pair. Scale bar = 10 μm. (B) Comparative genomic hybridization (CGH) on a spermatogonial mitotic metaphase prepared from the testes of a sub-adult male (left panel). Chromosomes were counterstained with DAPI (blue). Female-derived genomic probe was labelled with fluorescein-12-dUTP (green) and male-derived genomic probe with Cy3-dUTP (red). Both probes highlighted one arm of the two large metacentric chromosomes (arrows) and the centromeric heterochromatin of all chromosomes (asterisks), but did not differentiate a sex chromosome pair as demonstrated in the constructed karyotype (right panel). Scale bar = 10 μm.

    Article Snippet: The probes were labelled using a Nick Translation Kit (Abbott Molecular Inc.); male DNA with Cy3-dUTP (GE Healthcare) and female DNA with fluorescein-12-dUTP (Invitrogen).

    Techniques: Staining, Construct, Hybridization, Derivative Assay

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Journal: Scientific Reports

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    doi: 10.1038/s41598-018-34545-x

    Figure Lengend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Article Snippet: Identification of L. mollis chromosomes by GISH L. mollis genomic DNA was labeled with fluorescein-12-dUTP (Thermo Scientific) using Random Primers DNA Labeling System (Invitrogen).

    Techniques: In Situ Hybridization