flow spectrum analyzer  (Cytek Biosciences)

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Assessing spectral resolvability of metabolic probes for multiparametric cytometry (A) Experimental workflow for evaluating probe co-detection. (B and D) Representative density plots demonstrating spectral overlap for pairwise probe combinations within the (B) FITC and (D) PE channel. (C and E) Complementary validation matrices showing computational spectral similarity scores from FluoroFinder (lower left quadrant) versus empirical resolvability determined by unmixing (upper right quadrant). Gray indicates non-resolvable pairs; colored tiles indicate resolvable pairs (C, green; E, red).

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Cytometry, Biomarker Discovery

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Validation framework for spectral co-resolvability of metabolic probes and fluorophore conjugates (A) Experimental workflow for co-detection assessment. (B, D, and F) Representative flow cytometry density plots demonstrating pairwise mixing between metabolic probes and fluorophores in the (B) FITC/AF488, (D) PE, and (F) APC/AF647 channel. (C, E, and G) Validation matrices per channel: upper, computational spectral similarity by FluoroFinder; lower, empirically determined resolvability. Gray tiles indicate non-resolvable pairs; colored tiles confirm resolvable pairs. (C) FITC/AF488, green; (E) PE, red; and (G) APC/AF647, blue.

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Biomarker Discovery, Flow Cytometry

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Validation of three metabolic probes for simultaneous assessment of mitochondrial activity and oxidative stress (A) Representative flow cytometry density plots demonstrating spectral resolution of co-stained probes. (B) Validation matrix: lower left quadrant, computational spectral similarity by FluoroFinder; upper right quadrant, empirical resolvability determination (red: resolvable pairs). (C) Mean fluorescence intensity (MFI; mean ± SD) of individual probes in control versus rotenone/antimycin A (Rot/AA)-treated groups ( n = 6). (D) Correlation analysis of MFI between single-stain and multiplexed conditions across probes (Spearman’s r). (E) MFI of probes (mean ± SD) in control versus EZH2-knockdown (EZH2-sh) groups under single and multiplexed staining. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Biomarker Discovery, Activity Assay, Flow Cytometry, Staining, Fluorescence, Control, Knockdown

Journal: ACS applied materials & interfaces

Article Title: Manganese-Loaded pH-Responsive DNA Hydrogels Enable Tg-Guided Thyroid Tumor Targeted Magnetic Resonance Imaging.

doi: 10.1021/acsami.4c19676

Figure Lengend Snippet: Figure 3. In vitro stability and cytotoxicity of M-TDH. (a) Tyndall effect showed good stability of M-TDH in saline with 10% FBS. (b) Cell viability after 24 h of incubation with PBS, TDH, M-TDH, and MnCl2, detected by CCK-8. (c, d) Cell apoptosis rates of DTC cells detected by flow cytometry. (e, f) Live/dead cell staining images and corresponding quantitative comparison of TPC1 and K1 cells after various treatments (scale bar 100 μm). (g, h) Hematological parameters of M-TDH detected by hemolysis experiments and corresponding quantitative measurements.

Article Snippet: The quantification of cellular uptake was obtained by full spectrum analysis flow cytometry (ID7000 Sony, Japan).

Techniques: In Vitro, Saline, Incubation, CCK-8 Assay, Flow Cytometry, Staining, Comparison

Journal: ACS applied materials & interfaces

Article Title: Manganese-Loaded pH-Responsive DNA Hydrogels Enable Tg-Guided Thyroid Tumor Targeted Magnetic Resonance Imaging.

doi: 10.1021/acsami.4c19676

Figure Lengend Snippet: Figure 5. Uptake efficiency and aptamer-mediated combination. (a, b) The uptake efficiency of M-TDH or TDH by K1 and TPC-1 cells detected by flow cytometry. (c) The in situ targeting of the nanohydrogel in frozen thyroid tissue sections. The white box shows the enlarged area, Cy5- TDH shows a red fluorescence signal, and a green fluorescence signal indicates the Tg distribution. (d) Nucleic acid−protein binding assay used to compare the combination between DH or TDH and endogenous Tg protein in the cellular environment.

Article Snippet: The quantification of cellular uptake was obtained by full spectrum analysis flow cytometry (ID7000 Sony, Japan).

Techniques: Flow Cytometry, In Situ, Fluorescence, Protein Binding