fitc conjugated goat anti mouse igg  (Thermo Fisher)


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  • 93

    Structured Review

    Thermo Fisher fitc conjugated goat anti mouse igg
    A. CTL007 migrates toward CCL2 in Transwell . T cells were placed in the top chamber of the Transwell. CCL2 (10 ng/ml) in T cell medium, or T cell medium alone were added to the bottom of the Transwell (duplicate wells). After 90 min of culture, the number of migrated cells in the bottom chamber was counted under the microscope. Some T cell cultures were pre-incubated and treated with saturating concentration (10 μg/ml) of mouse anti-CCR2 Ab or mouse <t>IgG</t> as control. B. Expression of CCR2. T cells grown in log phase were incubated with either anti-CCR2 or anti-CXCR3 Ab (black or grey solid line) or mouse IgG control (dotted line) in RPMI 1640 with 5% human AB serum for 1 h at 4°C. After washing, <t>FITC-labeled</t> anti-mouse IgG was added. Expression of chemokine receptors was detected by flow cytometry.
    Fitc Conjugated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated goat anti mouse igg/product/Thermo Fisher
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated goat anti mouse igg - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2"

    Article Title: In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2

    Journal: Journal of Translational Medicine

    doi: 10.1186/1479-5876-9-33

    A. CTL007 migrates toward CCL2 in Transwell . T cells were placed in the top chamber of the Transwell. CCL2 (10 ng/ml) in T cell medium, or T cell medium alone were added to the bottom of the Transwell (duplicate wells). After 90 min of culture, the number of migrated cells in the bottom chamber was counted under the microscope. Some T cell cultures were pre-incubated and treated with saturating concentration (10 μg/ml) of mouse anti-CCR2 Ab or mouse IgG as control. B. Expression of CCR2. T cells grown in log phase were incubated with either anti-CCR2 or anti-CXCR3 Ab (black or grey solid line) or mouse IgG control (dotted line) in RPMI 1640 with 5% human AB serum for 1 h at 4°C. After washing, FITC-labeled anti-mouse IgG was added. Expression of chemokine receptors was detected by flow cytometry.
    Figure Legend Snippet: A. CTL007 migrates toward CCL2 in Transwell . T cells were placed in the top chamber of the Transwell. CCL2 (10 ng/ml) in T cell medium, or T cell medium alone were added to the bottom of the Transwell (duplicate wells). After 90 min of culture, the number of migrated cells in the bottom chamber was counted under the microscope. Some T cell cultures were pre-incubated and treated with saturating concentration (10 μg/ml) of mouse anti-CCR2 Ab or mouse IgG as control. B. Expression of CCR2. T cells grown in log phase were incubated with either anti-CCR2 or anti-CXCR3 Ab (black or grey solid line) or mouse IgG control (dotted line) in RPMI 1640 with 5% human AB serum for 1 h at 4°C. After washing, FITC-labeled anti-mouse IgG was added. Expression of chemokine receptors was detected by flow cytometry.

    Techniques Used: Microscopy, Incubation, Concentration Assay, Expressing, Labeling, Flow Cytometry, Cytometry

    Related Articles

    Positive Control:

    Article Title: An efficient strategy for cell-based antibody library selection using an integrated vector system
    Article Snippet: .. For comparison, a monoclonal antibody (mAb) against CD9, clone MM2/57 (Merck millipore) was used as positive control, and its binding to the cells was detected using FITC-conjugated goat anti-mouse IgG (Pierce). .. Immunoprecipitation and immunoblotting The binding specificity of scFv-Fc to CD9 was tested by immunoprecipitation and immunoblotting.

    Fluorescence:

    Article Title: Identification and Expression of a Mycoplasma gallisepticum Surface Antigen Recognized by a Monoclonal Antibody Capable of Inhibiting Both Growth and Metabolism
    Article Snippet: .. Bound MAb was detected with FITC-conjugated goat anti-mouse IgG (Life Technologies, Inc.) by fluorescence microscopy. .. The nucleotide sequence of the mgc3 gene has been deposited with GenBank under accession number .

    Microscopy:

    Article Title: Identification and Expression of a Mycoplasma gallisepticum Surface Antigen Recognized by a Monoclonal Antibody Capable of Inhibiting Both Growth and Metabolism
    Article Snippet: .. Bound MAb was detected with FITC-conjugated goat anti-mouse IgG (Life Technologies, Inc.) by fluorescence microscopy. .. The nucleotide sequence of the mgc3 gene has been deposited with GenBank under accession number .

    Incubation:

    Article Title: Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow
    Article Snippet: .. Microspheres or cells (2 × 106 ) were incubated with 10 μg/ml PL1, HECA-452, or isotype-matched control murine IgG1 or rat IgM for 30 min, washed, and incubated with FITC-conjugated goat anti–mouse IgG (H + L; Caltag) or FITC-conjugated goat anti–rat IgM (Pharmingen) for 30 min. To measure the density of mAb-targeted 2-GSP-6, cells were incubated with 10 μg/ml biotinylated PL1 for 30 min, washed, and then incubated with FITC-conjugated streptavidin for 30 min. Control incubations omitted biotinylated 2-GSP-6 or biotinylated PL1. sP-selectin dimerized by preincubation with nonblocking anti-P-selectin mAb S12 (22 μg/ml sP-selectin and 5 μg/ml S12 for 1 h) in the presence or absence of 50 μg/ml blocking anti–P-selectin mAb G1 was incubated with microspheres or cells for 1 h, followed by washing and incubation with FITC-conjugated goat anti–mouse IgG. .. Binding was analyzed on a Becton-Dickinson FACscan using CellQuest software.

    Article Title: The RodA hydrophobin on Aspergillus fumigatus spores masks Dectin-1 and Dectin-2 dependent responses and enhances fungal survival in vivo
    Article Snippet: .. Conidia were centrifuged (10,000rpm, 5min) and blocked with 1.5% normal rabbit serum in PBS for 1h, then incubated with a Dectin-1 Fc fusion protein ( )), which was a gift from Dr Chad Steele, University of Alabama at Birmingham Conidia were then washed 3x with PBS and incubated with FITC-conjugated goat-anti-mouse IgG (Invitrogen) diluted to 1mg/ ml in PBS for 1h at 37°C. .. A. fumigatus strains were cultured in VMM agar in 25cm2 tissue culture flasks.

    Article Title: Targeting Swine Leukocyte Antigen Class I Molecules for Proteasomal Degradation by the nsp1α Replicase Protein of the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Strain JXwn06
    Article Snippet: .. After being washed 3 times with PBS for 5 min each, the cells were then incubated with Alexa Fluor 568-conjugated goat anti-mouse IgG F(ab′)2 fragment or FITC-conjugated goat anti-mouse IgG (Molecular Probes) secondary antibody for an additional 1 h. Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). .. The images were collected with an Olympus confocal microscope.

    Blocking Assay:

    Article Title: Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow
    Article Snippet: .. Microspheres or cells (2 × 106 ) were incubated with 10 μg/ml PL1, HECA-452, or isotype-matched control murine IgG1 or rat IgM for 30 min, washed, and incubated with FITC-conjugated goat anti–mouse IgG (H + L; Caltag) or FITC-conjugated goat anti–rat IgM (Pharmingen) for 30 min. To measure the density of mAb-targeted 2-GSP-6, cells were incubated with 10 μg/ml biotinylated PL1 for 30 min, washed, and then incubated with FITC-conjugated streptavidin for 30 min. Control incubations omitted biotinylated 2-GSP-6 or biotinylated PL1. sP-selectin dimerized by preincubation with nonblocking anti-P-selectin mAb S12 (22 μg/ml sP-selectin and 5 μg/ml S12 for 1 h) in the presence or absence of 50 μg/ml blocking anti–P-selectin mAb G1 was incubated with microspheres or cells for 1 h, followed by washing and incubation with FITC-conjugated goat anti–mouse IgG. .. Binding was analyzed on a Becton-Dickinson FACscan using CellQuest software.

    Staining:

    Article Title: Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China
    Article Snippet: .. The FITC-conjugated goat anti-mice IgG (Thermo Fisher Scientific, USA) was used as the secondary antibody followed by DAPI staining. .. 2.6 Genomic cloning and bioinformatics analyses Total RNA was extracted from the isolated virus with TRIzol reagent, and cDNAs were subsequently amplified by SuperScript II with specific primers according to the manufacturer’s instructions (Thermo Fisher Scientific).

    Article Title: Induction of Lymphocyte Apoptosis by Tumor Cell Secretion of FasL-bearing Microvesicles
    Article Snippet: .. After mechanical detachment, cells were stained with anti-FasL mAbs (clone G247 and clone NOK-1; both from BD PharMingen), recognizing two different epitopes of the FasL molecule, and with FITC-conjugated goat anti–mouse IgG (Biosource International). .. As negative control, an isotype-matched mAb followed by the FITC-conjugated anti–mouse IgG was used.

    Article Title: Targeting Swine Leukocyte Antigen Class I Molecules for Proteasomal Degradation by the nsp1α Replicase Protein of the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Strain JXwn06
    Article Snippet: .. After being washed 3 times with PBS for 5 min each, the cells were then incubated with Alexa Fluor 568-conjugated goat anti-mouse IgG F(ab′)2 fragment or FITC-conjugated goat anti-mouse IgG (Molecular Probes) secondary antibody for an additional 1 h. Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). .. The images were collected with an Olympus confocal microscope.

    Binding Assay:

    Article Title: An efficient strategy for cell-based antibody library selection using an integrated vector system
    Article Snippet: .. For comparison, a monoclonal antibody (mAb) against CD9, clone MM2/57 (Merck millipore) was used as positive control, and its binding to the cells was detected using FITC-conjugated goat anti-mouse IgG (Pierce). .. Immunoprecipitation and immunoblotting The binding specificity of scFv-Fc to CD9 was tested by immunoprecipitation and immunoblotting.