flow cytometry anti he selectin fluorescein conjugated mouse igg1  (R&D Systems)

 
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    Name:
    Mouse IgG1 Antibody
    Description:
    The Mouse IgG1 Antibody from R D Systems is a rabbit monoclonal antibody to IgG1 This antibody reacts with mouse The Mouse IgG1 Antibody has been validated for the following applications Western Blot Flow Cytometry
    Catalog Number:
    mab9627-100
    Price:
    279
    Applications:
    Western Blot, Flow Cytometry
    Host:
    Rabbit
    Purity:
    Protein A or G purified from cell culture supernatant
    Conjugate:
    Unconjugated
    Immunogen:
    Mouse IgG
    Size:
    100 ug
    Category:
    Secondary Antibodies
    Isotype:
    IgG
    Buy from Supplier


    Structured Review

    R&D Systems flow cytometry anti he selectin fluorescein conjugated mouse igg1
    Mouse IgG1 Antibody
    The Mouse IgG1 Antibody from R D Systems is a rabbit monoclonal antibody to IgG1 This antibody reacts with mouse The Mouse IgG1 Antibody has been validated for the following applications Western Blot Flow Cytometry
    https://www.bioz.com/result/flow cytometry anti he selectin fluorescein conjugated mouse igg1/product/R&D Systems
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometry anti he selectin fluorescein conjugated mouse igg1 - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium"

    Article Title: Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1601219

    BMP6 increases surface expression of E-selectin, VCAM-1 and ICAM-1 on LPS-stimulated blood outgrowth endothelial cells (BOECs). BOECs were treated with BMP6 (16 h; 50 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to
    Figure Legend Snippet: BMP6 increases surface expression of E-selectin, VCAM-1 and ICAM-1 on LPS-stimulated blood outgrowth endothelial cells (BOECs). BOECs were treated with BMP6 (16 h; 50 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to

    Techniques Used: Expressing, Flow Cytometry, Cytometry

    BMP9 increases surface expression of E-selectin and VCAM-1, but not ICAM-1 on LPS-stimulated ECs. ECs were treated with BMP9 (16 h; 5 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to assess surface expression of E-selectin
    Figure Legend Snippet: BMP9 increases surface expression of E-selectin and VCAM-1, but not ICAM-1 on LPS-stimulated ECs. ECs were treated with BMP9 (16 h; 5 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to assess surface expression of E-selectin

    Techniques Used: Expressing, Flow Cytometry, Cytometry

    Related Articles

    Clone Assay:

    Article Title: Membrane-bound p35 Subunit of IL-12 on Tumor Cells is Functionally Equivalent to Membrane-bound Heterodimeric Single Chain IL-12 for Induction of Anti-tumor Immunity
    Article Snippet: .. Antibodies and reagents Anti-IL-12 p35 monoclonal mouse IgG1 antibody, (R & D systems, Minneapolis, MN, USA) and FITC-conjugated anti-mouse IgG antibody (Sigma-Aldrich, St. Louis, MO, USA) were used to detect expression of IL-12 fusion proteins on the cell surface of MethA tumor clones. .. Red blood cell lysis buffer, Concanavalin A (ConA) was purchased from Sigma-Aldrich, Mitomycin-C from Enzo life sciences (Farmingdale, NY, USA), and G418 (gentamycin) from Calbiochem (San Diego, CA, USA).

    Flow Cytometry:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 106 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R & D Systems Inc., Minneapolis, MN, ) after being washed in staining buffer for 5 minutes. .. Then, Fc receptors were blocked and samples were transferred into a 5-ml flow cytometry tube and incubated with each antibody or each corresponding isotype control antibody at a concentration of 1:100.

    Article Title: Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium
    Article Snippet: .. Mouse monoclonal antibodies for flow cytometry: Anti-hE-selectin Fluorescein Conjugated mouse IgG1 (anti-human E-selectin-FITC; R & D Systems), Allophycocyanin (APC) mouse anti-human CD54 (anti-human ICAM-1-APC; BD Pharmingen), and PE/Cy5 anti-human CD106 (anti-human VCAM-1-PECy5; BioLegend). .. Isotype control antibodies used for flow cytometry: APC-mouse IgG1 (BD Pharmingen), Mouse IgG1 Isotype control Fluorescein (R & D Systems) and PE/Cy5 mouse IgG1 Isotype (BioLegend).

    Cytometry:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 106 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R & D Systems Inc., Minneapolis, MN, ) after being washed in staining buffer for 5 minutes. .. Then, Fc receptors were blocked and samples were transferred into a 5-ml flow cytometry tube and incubated with each antibody or each corresponding isotype control antibody at a concentration of 1:100.

    Article Title: Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium
    Article Snippet: .. Mouse monoclonal antibodies for flow cytometry: Anti-hE-selectin Fluorescein Conjugated mouse IgG1 (anti-human E-selectin-FITC; R & D Systems), Allophycocyanin (APC) mouse anti-human CD54 (anti-human ICAM-1-APC; BD Pharmingen), and PE/Cy5 anti-human CD106 (anti-human VCAM-1-PECy5; BioLegend). .. Isotype control antibodies used for flow cytometry: APC-mouse IgG1 (BD Pharmingen), Mouse IgG1 Isotype control Fluorescein (R & D Systems) and PE/Cy5 mouse IgG1 Isotype (BioLegend).

    Incubation:

    Article Title: Induction of tumour necrosis factor receptor-expressing macrophages by interleukin-10 and macrophage colony-stimulating factor in rheumatoid arthritis
    Article Snippet: .. PBMC suspensions were incubated with mouse IgG1 anti-IL-10R1 monoclonal antibody (mAb) (R & D Systems, Minneapolis, MN, USA), mouse IgG1 anti-IL-10R2 mAb (R & D Systems), rabbit IgG anti-M-CSFR (c-fms) polyclonal Ab (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), mouse IgG2a anti-TNFR1 mAb (BD Biosciences, San Jose, CA, USA), fluorescein isothiocyanate (FITC)-conjugated mouse IgG2a anti-TNFR2 mAb (Genzyme/Techne, Minneapolis, MN, USA), or isotype-matched control Abs. .. Cells were washed and then incubated with rhodamine-conjugated goat anti-mouse IgG1 mAb (Santa Cruz Biotechnology, Inc.) for anti-IL-10R1 mAb and anti-IL-10R2 mAb, phycoerythrin-conjugated goat anti-rabbit IgG mAb (Santa Cruz Biotechnology, Inc.) for anti-M-CSF Ab, or FITC-conjugated anti-mouse IgG2a mAb (Santa Cruz Biotechnology, Inc.) for anti-TNFR1 mAb.

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 106 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R & D Systems Inc., Minneapolis, MN, ) after being washed in staining buffer for 5 minutes. .. Then, Fc receptors were blocked and samples were transferred into a 5-ml flow cytometry tube and incubated with each antibody or each corresponding isotype control antibody at a concentration of 1:100.

    Expressing:

    Article Title: Membrane-bound p35 Subunit of IL-12 on Tumor Cells is Functionally Equivalent to Membrane-bound Heterodimeric Single Chain IL-12 for Induction of Anti-tumor Immunity
    Article Snippet: .. Antibodies and reagents Anti-IL-12 p35 monoclonal mouse IgG1 antibody, (R & D systems, Minneapolis, MN, USA) and FITC-conjugated anti-mouse IgG antibody (Sigma-Aldrich, St. Louis, MO, USA) were used to detect expression of IL-12 fusion proteins on the cell surface of MethA tumor clones. .. Red blood cell lysis buffer, Concanavalin A (ConA) was purchased from Sigma-Aldrich, Mitomycin-C from Enzo life sciences (Farmingdale, NY, USA), and G418 (gentamycin) from Calbiochem (San Diego, CA, USA).

    Article Title: Immunophenotypes in the circulation of patients with mild cognitive impairment
    Article Snippet: .. Surface expression of APP and intracellular expression of Aβ in lymphocytes and monocytes were determined using a mouse IgG1 monoclonal antibody directed against the epitope within amino acids L18-R288 or P365-R411 of APP (R & D Systems, Minneapolis, MN), and a mouse IgG1 monoclonal antibody recognizing an epitope within amino acid residues 1-12 of the Aβ peptide (clone BAM-10, Sigma, Saint Louis, MO). .. FITC labeled goat anti-mouse IgG antibody (GAM-FITC; Pierce, Rockford, IL) was used as the secondary fluorescent label for both surface APP and intracellular Aβ.

    Staining:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 106 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R & D Systems Inc., Minneapolis, MN, ) after being washed in staining buffer for 5 minutes. .. Then, Fc receptors were blocked and samples were transferred into a 5-ml flow cytometry tube and incubated with each antibody or each corresponding isotype control antibody at a concentration of 1:100.

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  • About
  • News
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  • Team
  • Advisors
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  • Contact
  • Bioz Stars
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  • 96
    R&D Systems flow cytometry anti he selectin fluorescein conjugated mouse igg1
    BMP6 increases surface expression of <t>E-selectin,</t> VCAM-1 and ICAM-1 on LPS-stimulated blood outgrowth endothelial cells (BOECs). BOECs were treated with BMP6 (16 h; 50 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow <t>cytometry</t> was performed to
    Flow Cytometry Anti He Selectin Fluorescein Conjugated Mouse Igg1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry anti he selectin fluorescein conjugated mouse igg1/product/R&D Systems
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    flow cytometry anti he selectin fluorescein conjugated mouse igg1 - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    BMP6 increases surface expression of E-selectin, VCAM-1 and ICAM-1 on LPS-stimulated blood outgrowth endothelial cells (BOECs). BOECs were treated with BMP6 (16 h; 50 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium

    doi: 10.4049/jimmunol.1601219

    Figure Lengend Snippet: BMP6 increases surface expression of E-selectin, VCAM-1 and ICAM-1 on LPS-stimulated blood outgrowth endothelial cells (BOECs). BOECs were treated with BMP6 (16 h; 50 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to

    Article Snippet: Mouse monoclonal antibodies for flow cytometry: Anti-hE-selectin Fluorescein Conjugated mouse IgG1 (anti-human E-selectin-FITC; R & D Systems), Allophycocyanin (APC) mouse anti-human CD54 (anti-human ICAM-1-APC; BD Pharmingen), and PE/Cy5 anti-human CD106 (anti-human VCAM-1-PECy5; BioLegend).

    Techniques: Expressing, Flow Cytometry, Cytometry

    BMP9 increases surface expression of E-selectin and VCAM-1, but not ICAM-1 on LPS-stimulated ECs. ECs were treated with BMP9 (16 h; 5 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to assess surface expression of E-selectin

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Bone morphogenetic protein 9 enhances lipopolysaccharide-induced leukocyte recruitment to the vascular endothelium

    doi: 10.4049/jimmunol.1601219

    Figure Lengend Snippet: BMP9 increases surface expression of E-selectin and VCAM-1, but not ICAM-1 on LPS-stimulated ECs. ECs were treated with BMP9 (16 h; 5 ng/ml) prior to the addition of LPS (4 h; 100 ng/ml). Flow cytometry was performed to assess surface expression of E-selectin

    Article Snippet: Mouse monoclonal antibodies for flow cytometry: Anti-hE-selectin Fluorescein Conjugated mouse IgG1 (anti-human E-selectin-FITC; R & D Systems), Allophycocyanin (APC) mouse anti-human CD54 (anti-human ICAM-1-APC; BD Pharmingen), and PE/Cy5 anti-human CD106 (anti-human VCAM-1-PECy5; BioLegend).

    Techniques: Expressing, Flow Cytometry, Cytometry