fisher s t4 ligase enzyme  (Thermo Fisher)


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    Name:
    T4 DNA Ligase 1 U µL
    Description:
    T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double stranded DNAs with 3 hydroxyl and 5 phosphate termini The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes Single stranded nucleic acids are not substrates for this enzyme ApplicationsCloning blunt end or cohesive end ligation and adding linkers or adapters to blunt ended DNASourcePurified from E coli lambda lysogen NM989Performance and quality testingEndodeoxyribonuclease 3 and 5 exodeoxyribonuclease assays ligation efficiency testedUnit definitionOne unit catalyzes the exchange of 1 nmol 32P labeled pyrophosphate into ATP in 20 min at 37°C One unit is equal to approximately 300 cohesive end ligation units Unit reaction conditions66 mM Tris HCl pH 7 6 6 6 mM MgCl2 10 mM DTT 66 µM ATP 3 3 µM 32P labeled pyrophosphate and enzyme in 0 1 mL for 20 min at 37°C
    Catalog Number:
    15224017
    Price:
    None
    Applications:
    ChIP-on-Chip|Cloning|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher fisher s t4 ligase enzyme
    T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double stranded DNAs with 3 hydroxyl and 5 phosphate termini The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes Single stranded nucleic acids are not substrates for this enzyme ApplicationsCloning blunt end or cohesive end ligation and adding linkers or adapters to blunt ended DNASourcePurified from E coli lambda lysogen NM989Performance and quality testingEndodeoxyribonuclease 3 and 5 exodeoxyribonuclease assays ligation efficiency testedUnit definitionOne unit catalyzes the exchange of 1 nmol 32P labeled pyrophosphate into ATP in 20 min at 37°C One unit is equal to approximately 300 cohesive end ligation units Unit reaction conditions66 mM Tris HCl pH 7 6 6 6 mM MgCl2 10 mM DTT 66 µM ATP 3 3 µM 32P labeled pyrophosphate and enzyme in 0 1 mL for 20 min at 37°C
    https://www.bioz.com/result/fisher s t4 ligase enzyme/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fisher s t4 ligase enzyme - by Bioz Stars, 2020-05
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer
    Article Snippet: .. The complete afa-1 operon (6.8 kb) was isolated by Xba I/Spe I restriction enzyme digest, purified by gel electrophoresis, ligated into pUC18 using T4 DNA-ligase and propagated in E coli One-ShotTOP10 (Invitrogen) selected on 100 µg/mL ampicillin agar. pUCAfa was then used to transform chemically competent E coli EPI300-T1 containing empty fosmid, to allow direct comparison with E coli 8H8. .. The presence and orientation of afa-1 within pUCAfa were confirmed by PCR.

    Chromatography:

    Article Title: New insight into the mechanism of mitochondrial cytochrome c function
    Article Snippet: .. Materials and methods Material used in this studies were: components for the culture media and buffer solutions for chromatography and electrophoresis (AppliChem, Germany), ampicillin, cytochrome c from equine heart (Sigma, United States), Xho I restriction endonuclease (Promega, USA), Bam H I restriction endonuclease (New England Biolabs Inc., USA), Pfu -DNA polymerase, and T4-DNA ligase (Fermentas, Lithuania). .. Distilled water was additionally purified on a Milli-Q system (Millipore, USA).

    Ligation:

    Article Title: Design of a New [PSI+]-No-More Mutation in SUP35 With Strong Inhibitory Effect on the [PSI+] Prion Propagation
    Article Snippet: .. Sticky-end ligation was performed with T4 DNA-ligase according to Thermo Scientific protocol. pRS315CG was obtained analogously from pRS316CG (Serio et al., ) and pRS315. pR16CUP-NM-yTagRFP-T plasmid was obtained by insertion of the XhoI-XhoI fragment from pCUP-NM-His6 (Kiktev et al., ) in place of the XhoI-SalI fragment of pR16CUP-SFP1C-yTagRFP-T which in turn resulted from the substitution of the PstI-PstI fragment in pR16CUP-SFP1-Cerulean (Matveenko et al., ) for the PstI-PstI fragment from pIM35 (Malcova et al., ). ..

    Isolation:

    Article Title: Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer
    Article Snippet: .. The complete afa-1 operon (6.8 kb) was isolated by Xba I/Spe I restriction enzyme digest, purified by gel electrophoresis, ligated into pUC18 using T4 DNA-ligase and propagated in E coli One-ShotTOP10 (Invitrogen) selected on 100 µg/mL ampicillin agar. pUCAfa was then used to transform chemically competent E coli EPI300-T1 containing empty fosmid, to allow direct comparison with E coli 8H8. .. The presence and orientation of afa-1 within pUCAfa were confirmed by PCR.

    Purification:

    Article Title: Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer
    Article Snippet: .. The complete afa-1 operon (6.8 kb) was isolated by Xba I/Spe I restriction enzyme digest, purified by gel electrophoresis, ligated into pUC18 using T4 DNA-ligase and propagated in E coli One-ShotTOP10 (Invitrogen) selected on 100 µg/mL ampicillin agar. pUCAfa was then used to transform chemically competent E coli EPI300-T1 containing empty fosmid, to allow direct comparison with E coli 8H8. .. The presence and orientation of afa-1 within pUCAfa were confirmed by PCR.

    Electrophoresis:

    Article Title: New insight into the mechanism of mitochondrial cytochrome c function
    Article Snippet: .. Materials and methods Material used in this studies were: components for the culture media and buffer solutions for chromatography and electrophoresis (AppliChem, Germany), ampicillin, cytochrome c from equine heart (Sigma, United States), Xho I restriction endonuclease (Promega, USA), Bam H I restriction endonuclease (New England Biolabs Inc., USA), Pfu -DNA polymerase, and T4-DNA ligase (Fermentas, Lithuania). .. Distilled water was additionally purified on a Milli-Q system (Millipore, USA).

    Incubation:

    Article Title: Inhibition of PTTG1 expression by microRNA suppresses proliferation and induces apoptosis of malignant glioma cells
    Article Snippet: .. The oligonucleotides (10 µM) were incubated in Invitrogen annealing buffer (Thermo Fisher Scientific, Inc.) at 95°C for 4 min and 37°C for 10 min prior to being ligated to the empty vector using Invitrogen T4 ligase (Thermo Fisher Scientific, Inc.). .. Recombinant vectors were used to transform competent cells, which were then seeded onto lysogeny broth (LB)-spectinomycin culture plates (BD Biosciences, Franklin Lakes, NJ, USA).

    other:

    Article Title: Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells
    Article Snippet: T4 DNA ligase (Cat. #15224–017) and T4 DNA polymerase (Cat. #18005–017) were from Invitrogen.

    Transformation Assay:

    Article Title: Nickel-induced HIF-1α promotes growth arrest and senescence in normal human cells but lacks toxic effects in transformed cells
    Article Snippet: .. Oligonucleotides containing targeting sequences and vector-compatible ends were ligated into the linearized pSUPER.retro.puro using T4 ligase (15224017, Invitrogen) overnight followed by the transformation of the plasmid products into One Shot TOPO10 Chemically Competent E. coli cells (C404003, Invitrogen). .. The viral particles were produced in 293T cells by cotransfection of pSUPER DNA with plasmids expressing MoMuLV gag-pol and VSVG.

    Plasmid Preparation:

    Article Title: Nickel-induced HIF-1α promotes growth arrest and senescence in normal human cells but lacks toxic effects in transformed cells
    Article Snippet: .. Oligonucleotides containing targeting sequences and vector-compatible ends were ligated into the linearized pSUPER.retro.puro using T4 ligase (15224017, Invitrogen) overnight followed by the transformation of the plasmid products into One Shot TOPO10 Chemically Competent E. coli cells (C404003, Invitrogen). .. The viral particles were produced in 293T cells by cotransfection of pSUPER DNA with plasmids expressing MoMuLV gag-pol and VSVG.

    Article Title: Inhibition of PTTG1 expression by microRNA suppresses proliferation and induces apoptosis of malignant glioma cells
    Article Snippet: .. The oligonucleotides (10 µM) were incubated in Invitrogen annealing buffer (Thermo Fisher Scientific, Inc.) at 95°C for 4 min and 37°C for 10 min prior to being ligated to the empty vector using Invitrogen T4 ligase (Thermo Fisher Scientific, Inc.). .. Recombinant vectors were used to transform competent cells, which were then seeded onto lysogeny broth (LB)-spectinomycin culture plates (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Design of a New [PSI+]-No-More Mutation in SUP35 With Strong Inhibitory Effect on the [PSI+] Prion Propagation
    Article Snippet: .. Sticky-end ligation was performed with T4 DNA-ligase according to Thermo Scientific protocol. pRS315CG was obtained analogously from pRS316CG (Serio et al., ) and pRS315. pR16CUP-NM-yTagRFP-T plasmid was obtained by insertion of the XhoI-XhoI fragment from pCUP-NM-His6 (Kiktev et al., ) in place of the XhoI-SalI fragment of pR16CUP-SFP1C-yTagRFP-T which in turn resulted from the substitution of the PstI-PstI fragment in pR16CUP-SFP1-Cerulean (Matveenko et al., ) for the PstI-PstI fragment from pIM35 (Malcova et al., ). ..

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  • News
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  • 95
    Thermo Fisher fisher s t4 ligase enzyme
    Fisher S T4 Ligase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fisher s t4 ligase enzyme/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fisher s t4 ligase enzyme - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    99
    Thermo Fisher t4 ligase
    T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Thermo Fisher
    Average 99 stars, based on 564 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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